# Copyright 2006-2009 by Peter Cock. All rights reserved. # This code is part of the Biopython distribution and governed by its # license. Please see the LICENSE file that should have been included # as part of this package. """ Bio.SeqIO support module (not for general use). Unless you are writing a new parser or writer for Bio.SeqIO, you should not use this module. It provides base classes to try and simplify things. """ from Bio.Alphabet import generic_alphabet class SequenceIterator: """Base class for building SeqRecord iterators. You should write a next() method to return SeqRecord objects. You may wish to redefine the __init__ method as well. """ def __init__(self, handle, alphabet=generic_alphabet): """Create a SequenceIterator object. handle - input file alphabet - optional, e.g. Bio.Alphabet.generic_protein Note when subclassing: - there should be a single non-optional argument, the handle. - you do not have to require an alphabet. - you can add additional optional arguments.""" self.handle = handle self.alphabet = alphabet ##################################################### # You may want to subclass this, for example # # to read through the file to find the first record,# # or if additional arguments are required. # ##################################################### def next(self): """Return the next record in the file. This method should be replaced by any derived class to do something useful.""" raise NotImplementedError("This object should be subclassed") ##################################################### # You SHOULD subclass this, to split the file up # # into your individual records, and convert these # # into useful objects, e.g. return SeqRecord object # ##################################################### def __iter__(self): """Iterate over the entries as a SeqRecord objects. Example usage for Fasta files: myFile = open("example.fasta","r") myFastaReader = FastaIterator(myFile) for record in myFastaReader: print record.id print record.seq myFile.close()""" return iter(self.next, None) class InterlacedSequenceIterator(SequenceIterator): """Base class for any iterator of a non-sequential file type. This object is not intended for use directly. When writing a parser for any interlaced sequence file where the whole file must be read in order to extract any single record, then you should subclass this object. All you need to do is to define your own: (1) __init__ method to parse the file and call self.move_start() (2) __len__ method to return the number of records (3) __getitem__ to return any requested record. This class will then provide the iterator methods including next(), but relies on knowing the total number of records and tracking the pending record index in as self._n It is up to the subclassed object to decide if it wants to generate a cache of SeqRecords when initialised, or simply use its own lists and dicts and create SeqRecords on request. """ def __init__(self): """Create the object. This method should be replaced by any derived class to do something useful.""" #We assume that your implementation of __init__ will ensure self._n=0 self.move_start() raise NotImplementedError("This object method should be subclassed") ##################################################### # You SHOULD subclass this # ##################################################### def __len__(self): """Return the number of records. This method should be replaced by any derived class to do something useful.""" raise NotImplementedError("This object method should be subclassed") ##################################################### # You SHOULD subclass this # ##################################################### def __getitem__(self, i): """Return the requested record. This method should be replaced by any derived class to do something useful. It should NOT touch the value of self._n""" raise NotImplementedError("This object method should be subclassed") ##################################################### # You SHOULD subclass this # ##################################################### def move_start(self): self._n = 0 def next(self): next_record = self._n if next_record < len(self): self._n = next_record+1 return self[next_record] else: #StopIteration return None def __iter__(self): return iter(self.next, None) class SequenceWriter: """This class should be subclassed. Interlaced file formats (e.g. Clustal) should subclass directly. Sequential file formats (e.g. Fasta, GenBank) should subclass the SequentialSequenceWriter class instead. """ def __init__(self, handle): """Creates the writer object. Use the method write_file() to actually record your sequence records.""" self.handle = handle def _get_seq_string(self, record): """Use this to catch errors like the sequence being None.""" try: #The tostring() method is part of the Seq API, we could instead #use str(record.seq) but that would give a string "None" if the #sequence was None, and unpredicatable output if an unexpected #object was present. return record.seq.tostring() except AttributeError: if record.seq is None: #We could silently treat this as an empty sequence, Seq(""), #but that would be an implict assumption we should avoid. raise TypeError("SeqRecord (id=%s) has None for its sequence." \ % record.id) else: raise TypeError("SeqRecord (id=%s) has an invalid sequence." \ % record.id) def clean(self, text): """Use this to avoid getting newlines in the output.""" return text.replace("\n", " ").replace("\r", " ").replace(" ", " ") def write_file(self, records): """Use this to write an entire file containing the given records. records - A list or iterator returning SeqRecord objects Should return the number of records (as an integer). This method can only be called once.""" #Note when implementing this, your writer class should NOT close the #file at the end, but the calling code should. raise NotImplementedError("This object should be subclassed") ##################################################### # You SHOULD subclass this # ##################################################### class SequentialSequenceWriter(SequenceWriter): """This class should be subclassed. It is intended for sequential file formats with an (optional) header, repeated records, and an (optional) footer. In this case (as with interlaced file formats), the user may simply call the write_file() method and be done. However, they may also call the write_header(), followed by multiple calls to write_record() and/or write_records() followed finally by write_footer(). Users must call write_header() and write_footer() even when the file format concerned doesn't have a header or footer. This is to try and make life as easy as possible when switching the output format. Note that write_header() cannot require any assumptions about the number of records. """ def __init__(self, handle): self.handle = handle self._header_written = False self._record_written = False self._footer_written = False def write_header(self): assert not self._header_written, "You have aleady called write_header()" assert not self._record_written, "You have aleady called write_record() or write_records()" assert not self._footer_written, "You have aleady called write_footer()" self._header_written = True def write_footer(self): assert self._header_written, "You must call write_header() first" assert self._record_written, "You have not called write_record() or write_records() yet" assert not self._footer_written, "You have aleady called write_footer()" self._footer_written = True def write_record(self, record): """Write a single record to the output file. record - a SeqRecord object Once you have called write_header() you can call write_record() and/or write_records() as many times as needed. Then call write_footer() and close().""" assert self._header_written, "You must call write_header() first" assert not self._footer_written, "You have already called write_footer()" self._record_written = True raise NotImplementedError("This object should be subclassed") ##################################################### # You SHOULD subclass this # ##################################################### def write_records(self, records): """Write multiple record to the output file. records - A list or iterator returning SeqRecord objects Once you have called write_header() you can call write_record() and/or write_records() as many times as needed. Then call write_footer() and close(). Returns the number of records written. """ #Default implementation: assert self._header_written, "You must call write_header() first" assert not self._footer_written, "You have already called write_footer()" count = 0 for record in records: self.write_record(record) count += 1 #Mark as true, even if there where no records self._record_written = True return count def write_file(self, records): """Use this to write an entire file containing the given records. records - A list or iterator returning SeqRecord objects This method can only be called once. Returns the number of records written. """ self.write_header() count = self.write_records(records) self.write_footer() return count