ID U87107 standard; DNA; SYN; 8840 BP. XX AC U87107; XX SV U87107.1 XX DT 15-OCT-1997 (Rel. 52, Created) DT 15-OCT-1997 (Rel. 52, Last updated, Version 4) XX DE Cloning vector pAL-F insertion sequence IS1 galactokinase (galK), DE aminoglycoside 3'-phosphotransferase (kn), beta-galactosidase (lacZ), small DE ribosomal protein and beta-lactamase (Ap) genes, complete cds. XX KW . XX OS Cloning vector pAL-F OC artificial sequence; vectors. XX RN [1] RP 1-8840 RA Ahmed A., Podemski L.; RT "Use of ordered deletions in genome sequencing"; RL Gene 197:367-373(1997). XX RN [2] RP 1-8840 RA Ahmed A.; RT ; RL Submitted (27-JAN-1997) to the EMBL/GenBank/DDBJ databases. RL Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, RL Canada XX DR REMTREMBL; AAC53713; AAC53713. DR REMTREMBL; AAC53714; AAC53714. DR REMTREMBL; AAC53715; AAC53715. DR REMTREMBL; AAC53716; AAC53716. DR REMTREMBL; AAC53717; AAC53717. XX FH Key Location/Qualifiers FH FT source 1..8840 FT /db_xref="taxon:56954" FT /organism="Cloning vector pAL-F" FT /insertion_seq="IS1" FT /specific_host="Escherichia coli" FT CDS complement(933..2081) FT /codon_start=1 FT /db_xref="REMTREMBL:AAC53713" FT /transl_table=11 FT /gene="galK" FT /product="galactokinase" FT /protein_id="AAC53713.1" FT /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLP FT CAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQ FT LRNNSFGGVDMVDHGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAEN FT QFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSE FT YNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASA FT LEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCI FT VALIPEELVPAVQQRVAEQYEAKTGIKETFYVCKPSQGAGQC" FT CDS 2875..3669 FT /codon_start=1 FT /db_xref="REMTREMBL:AAC53714" FT /transl_table=11 FT /gene="kn" FT /function="kanamycin resistance" FT /product="aminoglycoside 3'-phosphotransferase" FT /protein_id="AAC53714.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT CDS 3978..4442 FT /codon_start=1 FT /db_xref="REMTREMBL:AAC53715" FT /note="alpha fragment" FT /transl_table=11 FT /gene="lacZ" FT /product="beta-galactosidase" FT /protein_id="AAC53715.1" FT /translation="MTMITNSCRRPRARGTVDGSLIKFKSPARRARVDGTTSLALAVVL FT QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDKLALYALMQF FT LCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWIDPAEFYADQ" FT CDS complement(4555..4929) FT /codon_start=1 FT /db_xref="REMTREMBL:AAC53716" FT /transl_table=11 FT /function="streptomycin sensitivity" FT /product="small ribosomal protein" FT /protein_id="AAC53716.1" FT /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKP FT NSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGALDC FT SGVKDRKQARSKYGVKRPKA" FT CDS complement(7087..7947) FT /codon_start=1 FT /db_xref="REMTREMBL:AAC53717" FT /transl_table=11 FT /gene="Ap" FT /function="ampicillin resistance" FT /product="beta-lactamase" FT /protein_id="AAC53717.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" XX SQ Sequence 8840 BP; 2068 A; 2288 C; 2319 G; 2165 T; 0 other; caattactgc aatgccctcg taattaagtg aatttacaat atcgtcctgt tcggagggaa 60 gaacgcggga tgttcattct tcatcacttt taattgatgt atatgctctc ttttctgacg 120 ttagtctccg acggcaggct tcaatgaccc aggctgagaa attcccggac cctttttgct 180 caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg cgggttgttg 240 ttctgcgggt tctgttcttc gttgacatga ggttgccccg tattcagtgt cgctgatttg 300 tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt 360 cataattgat tatttgacgt ggtttgatgg cctccacgca cgttgtgata tgtagatgat 420 aatcattatc actttacggg tcctttccgg tgatccgaca ggttacgggg cggcgacctc 480 gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca 540 taacttaatg tttttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa 600 gcgaggcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg 660 gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga gtatccgtac cattcagaac 720 tggcaggaac agggaatgcc cgttctgcga ggcggtggca agggtaatga ggtgctttat 780 gactctgccg ccgtcataaa atggtatgcc gaaagggatg ctgaaattga gaacgaaaag 840 ctgcgccggg aggttgaaga actgcggcag gccagcgagg cagatcaaca gtcggtacgg 900 ctgaccatcg ggtgccagtg cgggagtttc gttcagcact gtcctgctcc ttgtgatggt 960 ttacaaacgt aaaaagtctc tttaatacct gtttttgctt catattgttc agcgacacgt 1020 tgctgtacgg caggcaccag ctcttccggg atcagcgcga cgatacagcc gccaaatccg 1080 ccgccggtca tgcgtacgcc acctttgtcg ccaatcacag ctttgacgat ttctaccaga 1140 gtgtcaattt gcggcacggt gatttcgaaa tcatcgcgca tagaggcatg agactccgcc 1200 atcaactcgc ccatacgttt caggtcgcct tgctccagcg cgctggcagc ttcaacggtg 1260 cgggcgtttt cagtcagtat atgacgcacg cgttttgcca cgatcgggtc cagttcatgc 1320 gcaacagcgt tgaactcttc aatggtgaca tcacgcaggg ctggctgctg gaagaaacgc 1380 gcaccggttt cgcactgttc acgacgggtg ttgtattcgc tgccaaccag ggtacgtttg 1440 aagttactgt tgatgatgac gacagccaca cctttgggca tggaaactgc tttggtcccc 1500 agtgagcggc aatcgatcag caaggcatga tctttcttgc cgagcgcgga aattagctga 1560 tccatgatcc cgcagttaca gcctacaaac tggttttctg cttcctgacc gttaagcgcg 1620 atttgtgcgc cgtccagcgg cagatgataa agctgctgca atacggttcc gaccgcgact 1680 tccagtgaag cggaagaact taacccggca ccctgcggca cattgccgtg atcaaccatg 1740 tccacgccgc cgaagctgtt gttacgcagt tgcagatgtt tcaccacgcc acgaacgtag 1800 ttagcccatt gatagttttc atgtgcgaca atgggcgcat cgagggaaaa ctcgtcgagc 1860 tgattttcat aatcggctgc catcacgcga actttacggt catcgcgtgg tgcacaactg 1920 atcacggttt gataatcaat cgcgcagggc agaacgaaac cgtcgttgta gtcggtgtgt 1980 tcaccaatca aattcacgcg gccaggcgcc tgaatggtgt gagtggcagg gtagccaaat 2040 gcgttggcaa acagagattg tgttttttct ttcagactca tttcttacac tccggattcg 2100 cgacggccta cagcaacctg ggcgatgtat ggcatcaggt tattcggaat gccttgcgga 2160 tcttcgccca tatcgcccga cggatgcgcg ccaaccgggt tgaagtagcg cacgagggca 2220 atgctccagt ccggctgggc tttttgcaga tcggtgagga tctgttccac catcagcttg 2280 cttttgccgt aagggctttg cggtgtgccg gtcgggaagc tataatgcgg tagtttatca 2340 cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc 2400 atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg 2460 ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg cgtgctgcta 2520 gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg gaacacgtag 2580 aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg 2640 acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga 2700 tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc 2760 tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc 2820 tgatggcgca ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgcatgatt 2880 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 2940 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 3000 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaagac 3060 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 3120 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 3180 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 3240 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 3300 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 3360 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcggatgcc cgacggcgag 3420 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 3480 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 3540 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 3600 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 3660 ttcttctgag cgggactctg gggttcgcga tgataagctg tcaaacatga gaattacaac 3720 ttatatcgta tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc 3780 tagaaatatt ttatctgatt cgattcatta atgcagctgg cacgacaggt ttcccgactg 3840 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 3900 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 3960 tcacacagga aacagctatg accatgatta cgaattcctg caggcggccg cgagctcgag 4020 gtaccgtcga cggatcctta attaaattta aatcaccggc gcgccgagct cgagtcgacg 4080 gtaccacaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 4140 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 4200 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgata 4260 agctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg 4320 accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg 4380 cgatcatggc gaccacaccc gtcctgtgga tcgatccggc agaattttac gctgaccaat 4440 gacgcgacga cgtggcatgg aaatactccg ttgttaattc aggattgtcc aaaactctac 4500 gagtttagtt tgacatttaa gttaaaacgt ttagccttac ttaacggaga accattaagc 4560 cttaggacgc ttcacgccat acttggaacg agcctgctta cggtctttaa cgccggagca 4620 gtcaagcgca ccacgtacgg tgtggtaacg aacacccggg aggtctttaa cacgaccgcc 4680 acggatcagg atcacggagt gctcctgcag gttgtgacct tcaccaccga tgtaggaagt 4740 cacttcgaaa ccgttagtca gacgaacacg gcatacttta cgcagcgcgg agttcggttt 4800 tttaggagtg gtagtatata cacgagtaca tacgccacgt ttttgcgggc atgcttccag 4860 cgcaggcacg ttgcttttcg caactttgcg agcacgtggt ttgcgtacca gctggttaac 4920 tgttgccatt aaatagctcc tggttttagc ttttgcttcg taaacacgta ataaaacgtc 4980 ctcacacaat atgaggacgc cgaattttag ggcgatgccg aaaaggtgtc aagaaatata 5040 caacgatccc gccatcatca ccaggccatc tggctggggt gcttaaccgt aagtctgacg 5100 aaatcagtat agtcaatgag aatgatgtcg ttcgaaattt gaccagtcaa acccaaacca 5160 acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct 5220 cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc 5280 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa 5340 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg 5400 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc 5460 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga 5520 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt 5580 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga 5640 gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg 5700 aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc 5760 agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct 5820 gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg 5880 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 5940 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 6000 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 6060 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 6120 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 6180 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 6240 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 6300 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 6360 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 6420 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 6480 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 6540 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 6600 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6660 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6720 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 6780 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6840 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6900 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6960 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 7020 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 7080 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 7140 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 7200 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 7260 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7320 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7380 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7440 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7500 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7560 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7620 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7680 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 7740 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7800 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7860 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7920 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7980 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 8040 aggggttccg cgcacatttc cccgaaaagt gcggtaatga ctccaactta ttgatagtgt 8100 tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt tgagaacgac 8160 agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt 8220 cgctgcgtat atcgcttgct gattacgtgc agctttccct tcaggcggga ttcatacagc 8280 ggccagccat ccgtcatcca tatcaccacg tcaaagggtg acagcaggct cataagacgc 8340 cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt ccggagactg 8400 tcatacgcgt aaaacagcca gcgctggcgc gatttagccc cgacatagcc ccactgttcg 8460 tccatttccg cgcagacgat gacgtcactg cccggctgta tgcgcgaggt taccgactgc 8520 ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata atgcgggctg 8580 ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc gtaccgggtt 8640 gagaagcggt gtaagtgaac tgcagttgcc atgttttacg gcagtgagag cagagatagc 8700 gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaacaggagg 8760 gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc 8820 agtaagttgg cagcatcacc 8840 //