LOCUS pBAD30 4923 bp DNA circular SOURCE ORGANISM COMMENT This file is best viewed with a free Vector NTI Viewer: COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTREPLTYPE|Phagemid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers rep_origin 1..843 /vntifkey="33" /label=P15A_ORI CDS complement(1082..1960) /vntifkey="4" /label=araC /note="araC regulator of the arabinose BAD promoter" misc_feature 1989..2006 /vntifkey="21" /label=operator\O2 /note="operator O2" promoter complement(2111..2139) /vntifkey="30" /label=araC\promoter /note="araC promoter" misc_feature 2147..2168 /vntifkey="21" /label=operator\O1 /note="operator O1" misc_feature 2190..2203 /vntifkey="21" /label=CAP\site /note="CAP site" misc_feature 2199..2237 /vntifkey="21" /label=operator\I2\and\I1 /note="operator I2 + I1" promoter 2236..2263 /vntifkey="30" /label=arabinose\BAD\promoter /note="arabinose pBAD promoter" misc_feature 2286..2348 /vntifkey="21" /label=MCS /note="multiple cloning site" terminator 2349..2774 /vntifkey="43" /label=rrnB\T1\T2 promoter 2829..2835 /vntifkey="30" /label=P(BLA) /note="bla P3 promoter" CDS 2867..3730 /vntifkey="4" /label=AP(R) rep_origin 3766..4224 /vntifkey="33" /label=M13\ORI /note="M13 origin" BASE COUNT 1240 a 1274 c 1181 g 1228 t ORIGIN 1 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct 61 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata 121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa 181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa 241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg 301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt 361 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca 421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca 481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta 541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca 601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac 661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt 721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca 781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata 841 tttgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata 901 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag 961 gatctgctca tgtttgacag cttatcatcg atgcataatg tgcctgtcaa atggacgaag 1021 cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca 1081 attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc 1141 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac 1201 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct 1261 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa 1321 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat 1381 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg 1441 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat 1501 ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa 1561 acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa 1621 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact 1681 ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga 1741 acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct 1801 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa 1861 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg 1921 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat 1981 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact 2041 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg 2101 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc 2161 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 2221 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt 2281 tttgggctag cgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat 2341 gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag 2401 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 2461 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 2521 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 2581 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 2641 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 2701 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 2761 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 2821 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 2881 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 2941 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 3001 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 3061 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg 3121 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 3181 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 3241 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 3301 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 3361 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc 3421 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 3481 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 3541 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 3601 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 3661 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 3721 ttggtaactg tcagaccaag tttactcata tatactttag attgatttac gcgccctgta 3781 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 3841 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 3901 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 3961 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat 4021 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 4081 aaacttgaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc 4141 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 4201 acaaaatatt aacgtttaca atttaaaagg atctaggtga agatcctttt tgataatctc 4261 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 4321 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 4381 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 4441 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 4501 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 4561 ttaccagtca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg 4621 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt 4681 cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca 4741 ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca 4801 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca 4861 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 4921 atg //