ENTRY EC 1.1.1.1 NAME Alcohol dehydrogenase Aldehyde reductase CLASS Oxidoreductases Acting on the CH-OH group of donors With NAD+ or NADP+ as acceptor SYSNAME Alcohol:NAD+ oxidoreductase REACTION Alcohol + NAD+ = Aldehyde or Ketone + NADH SUBSTRATE NAD+ Primary alcohol Secondary alcohol Cyclic secondary alcohol Hemiacetal PRODUCT Aldehyde Ketone NADH COFACTOR Zinc COMMENT A zinc protein. Acts on primary or secondary alcohols or hemiacetals; the animal, but not the yeast, enzyme acts also on cyclic secondary alcohols The insect enzyme is a member of the nonmetallo-short-chain alcohol dehydrogenase (ADH) family (Proc.Natl.Acad.Sci.USA(1991) 88, 10064-10068). PATHWAY PATH: MAP00010 Glycolysis / Gluconeogenesis PATH: MAP00071 Fatty acid metabolism PATH: MAP00120 Bile acid biosynthesis PATH: MAP00350 Tyrosine metabolism PATH: MAP00561 Glycerolipid metabolism GENES ECO: b0356(adhC) b1241(adhE) b1478(adhP) b3589(yiaY) ECE: Z0456(adhC) Z2016(adhE) Z2232(adhP) Z5010(yiaY) ECS: ECs0411 ECs1741 ECs2082 ECs4466 HIN: HI0185(adhC) XFA: XF1746 XF2389 VCH: VC1147 VC2033 PAE: PA2119 PA2158 PA2188 PA3629(adhC) PA5427(adhA) PMU: PM1453(adh2) NME: NMB0546 NMB1304 NMA: NMA0725(adhA) NMA1518(adhC) MLO: mlr0872 mlr1136 mlr1178 CCR: CC2516 CC3029 BSU: BS2776(adhB) BS3211(gbsB) BHA: BH1829(adhB) LLA: L13145(adhE) L55758(adhA) SPY: SPy0044(adhA) MTU: Rv0761c(adhB) Rv1862(adhA) Rv2259(adhE2) MLE: ML1784(adhE2) ML2053 SYN: sll0990 AAE: aq_1240(adh2) aq_1362(adh1) TMA: TM0111 TM0920 AFU: AF0024 AF0339 AF2019 AF2101 HAL: VNG1821G(adh4) TAC: Ta0832 Ta0833 Ta0841 TVO: TVG0387048 TVG0995648 TVG1348694 PHO: PH0743 PAB: PAB1511 APE: APE1245 APE1557 APE1963 APE2239 SSO: SSO0472(adh-1) SSO0764(adh-2) SSO1220(adh-3) SSO1300(adh-4) SSO1646(adh-5) SSO2334(adh-6) SSO2441(adh-7) SSO2494(adh-8) SSO2501(adh-9) SSO2536(adh-10) SSO2717(adh-11) SSO2800(adh-12) SSO2878(adh-13) SCE: YBR145W(ADH5) YDL168W(SFA1) YGL256W(ADH4) YMR083W(ADH3) YMR303C(ADH2) YOL086C(ADH1) SPO: ADH1(adh1) SPAC5H10.06C(spac5h10.06c) SPCC13B11.04C ATH: At1g77120(F22K20_19) CEL: K12G11.3 K12G11.4 DME: CG3425(T3dh) CG3481(Adh) CG6598(Fdh) MMU: 1098256(Daq1) 87921(Adh1) 87926(Adh3) 87929(Adh5) HSA: 124(ADH1) 125(ADH2) 126(ADH3) 127(ADH4) 128(ADH5) 130(ADH6) 131(ADH7) DISEASE MIM: 103700 Alcohol dehydrogenase (class I), alpha polypeptide MIM: 103720 Alcohol dehydrogenase (class I), beta polypeptide MIM: 103730 Alcohol dehydrogenase (class I), gamma polypeptide MIM: 103740 Alcohol dehydrogenase (class II), pi polypeptide MIM: 600086 Alcohol dehydrogenase-7 STRUCTURES PDB: 1A4U 1A71 1A72 1ADB 1ADC 1ADF 1ADG 1AGN 1AXE 1AXG 1B14 1B15 1B16 1B2L 1BTO 1CDO 1D1S 1D1T 1DDA 1DEH 1E3E 1E3I 1E3L 1EE2 1HDX 1HDY 1HDZ 1HET 1HEU 1HF3 1HLD 1HSO 1HSZ 1HT0 1HTB 1LDE 1LDY 1QLH 1QLJ 1TEH 2OHX 2OXI 3BTO 3HUD 5ADH 6ADH 7ADH DBLINKS IUBMB Enzyme Nomenclature: 1.1.1.1 ExPASy - ENZYME nomenclature database: 1.1.1.1 WIT (What Is There) Metabolic Reconstruction: 1.1.1.1 BRENDA, the Enzyme Database: 1.1.1.1 SCOP (Structural Classification of Proteins): 1.1.1.1 /// ENTRY EC 1.1.1.62 NAME Estradiol 17beta-dehydrogenase CLASS Oxidoreductases Acting on the CH-OH group of donors With NAD+ or NADP+ as acceptor SYSNAME Estradiol-17beta:NAD(P)+ 17-oxidoreductase REACTION Estradiol-17beta + NAD+ or NADP+ = Estrone + NADH or NADPH SUBSTRATE Estradiol-17beta (S)-20-Hydroxypregn-4-en-3-one NAD+ NADP+ PRODUCT Estrone (S)-Pregn-4-en-3,20-dione NADH NADPH COMMENT Also acts on (S)-20-hydroxypregn-4-en-3-one and related compounds, oxidizing the (S)-20-group. B-specific with respect to NAD(P)+ (cf. EC 1.1.1.149). PATHWAY PATH: MAP00150 Androgen and estrogen metabolism GENES CEL: F11A5.12 DME: CG1444 CG3415 HSA: 3292(HSD17B1) 3293(HSD17B3) 3294(HSD17B2) 3295(HSD17B4) 3296(HSD17BP1) 51478(HSD17B7) DISEASE MIM: 109685 Hydroxysteroid (17-beta) dehydrogenase 2 MIM: 264300 Hydroxysteroid (17-beta) dehydrogenase 3 STRUCTURES PDB: 1FDT 1FDS 1FDV 1FDW 1IOL 3DHE 1EQU 1DHT 1BHS 1A27 1FDU DBLINKS IUBMB Enzyme Nomenclature: 1.1.1.62 ExPASy - ENZYME nomenclature database: 1.1.1.62 WIT (What Is There) Metabolic Reconstruction: 1.1.1.62 BRENDA, the Enzyme Database: 1.1.1.62 SCOP (Structural Classification of Proteins): 1.1.1.62 /// ENTRY EC 1.1.1.68 NAME Transferred to EC 1.7.99.5 CLASS Oxidoreductases Acting on the CH-OH group of donors With NAD+ or NADP+ as acceptor COMMENT Transferred entry. Now EC 1.7.99.5 - 5,10-Methylenetetrahydrofolate reductase (FADH2). DBLINKS IUBMB Enzyme Nomenclature: 1.1.1.68 ExPASy - ENZYME nomenclature database: 1.1.1.68 WIT (What Is There) Metabolic Reconstruction: 1.1.1.68 /// ENTRY EC 1.6.5.3 NAME NADH dehydrogenase (ubiquinone) Ubiquinone reductase Type I dehydrogenase Complex I dehydrogenase CLASS Oxidoreductases Acting on NADH or NADPH With quinone or related compound as acceptor SYSNAME NADH:ubiquinone oxidoreductase REACTION NADH + Ubiquinone = NAD+ + Ubiquinol SUBSTRATE NADH Ubiquinone PRODUCT NAD+ Ubiquinol COFACTOR FAD Iron Sulfur COMMENT A flavoprotein (FAD) containing iron-sulfur contres. The complex, present in mitochondria, can be degraded to form EC 1.6.99.3. PATHWAY PATH: MAP00130 Ubiquinone biosynthesis PATH: MAP00190 Oxidative phosphorylation GENES ECO: b2276(nuoN) b2277(nuoM) b2278(nuoL) b2279(nuoK) b2280(nuoJ) b2281(nuoI) b2282(nuoH) b2283(nuoG) b2284(nuoF) b2285(nuoE) b2286 b2287(nuoB) b2288(nuoA) ECE: Z3534(nuoN) Z3536(nuoM) Z3537(nuoL) Z3538(nuoK) Z3539(nuoJ) Z3540(nuoI) Z3541(nuoH) Z3542(nuoG) Z3543(nuoF) Z3544(nuoE) Z3545(nuoC) Z3546(nuoB) Z3547(nuoA) ECS: ECs3160 ECs3161 ECs3162 ECs3163 ECs3164 ECs3165 ECs3166 ECs3167 ECs3168 ECs3169 ECs3170 ECs3171 ECs3172 XFA: XF0305 XF0306 XF0307 XF0308 XF0309 XF0310 XF0311 XF0312 XF0313 XF0314 XF0315 XF0316 XF0317 XF0318 VCH: VC1581 VCA0155 VCA0157 PAE: PA1054 PA1056 PA1883 PA2637(nuoA) PA2638(nuoB) PA2639(nuoD) PA2640(nuoE) PA2641(nuoF) PA2642(nuoG) PA2643(nuoH) PA2644(nuoI) PA2645(nuoJ) PA2646(nuoK) PA2647(nuoL) PA2648(nuoM) PA2649(nuoN) BUC: BU154(nuoA) BU155(nuoB) BU156(nuoCD) BU157(nuoE) BU158(nuoF) BU159(nuoG) BU160(nuoH) BU161(nuoI) BU162(nuoJ) BU163(nuoK) BU164(nuoL) BU165(nuoM) BU166(nuoN) NME: NMB0241 NMB0242 NMB0243 NMB0244 NMB0245 NMB0246 NMB0249 NMB0250 NMB0251 NMB0253 NMB0254 NMB0257 NMB0258 NMB0259 NMA: NMA0002(nuoL) NMA0005(nuoK) NMA0006(nuoJ) NMA0008(nuoI) NMA0009(nuoH) NMA0010(nuoG) NMA0014(nuoF) NMA0015(nuoE) NMA0016(nuoD) NMA0017(nuoC) NMA0018(nuoB) NMA0019(nuoA) NMA0747(nqrF) NMA2228(nuoN) NMA2229(nuoM) HPY: HP1260(NQO7) HP1261(NQO6) HP1262(NQO5) HP1263 HP1264 HP1265 HP1266(NQO3) HP1267(NQO8) HP1268(NQO9) HP1269(NQO10) HP1270 HP1271(NQO12) HP1272(NQO13) HP1273(NQO14) HPJ: jhp1181 jhp1182 jhp1183 jhp1184 jhp1185 jhp1186 jhp1187 jhp1188 jhp1189 jhp1190 jhp1191 jhp1192 jhp1193 jhp1194 CJE: Cj1566c(nuoN) Cj1567c(nuoM) Cj1568c(nuoL) Cj1569c(nuoK) Cj1570c(nuoJ) Cj1571c(nuoI) Cj1572c(nuoH) Cj1573c(nuoG) Cj1574c Cj1575c Cj1576c(nuoD) Cj1577c(nuoC) Cj1578c(nuoB) Cj1579c(nuoA) RPR: RP115(nuoF) RP282(nuoL3) RP283(nuoL2) RP284(nuoN2) RP353(nuoE) RP354(nuoD) RP355(nuoC) RP356(nuoB) RP357(nuoA) RP537(nuoN1) RP790(nuoJ) RP791(nuoK) RP792(nuoL1) RP793(nuoM) RP795(nuoI) RP796(nuoH) RP797(nuoG) MLO: mll1352 mll1354 mll1355 mll1357 mll1358 mll1359 mll1361 mll1362 mll1364 mll1365 mll1366 mll1367 mll1369 mll1371 mll1372 mll5193 mll5397 mll5398 CCR: CC1937 CC1938 CC1939 CC1940 CC1941 CC1942 CC1945 CC1946 CC1947 CC1950 CC1952 CC1954 CC1955 CC1956 BSU: BS0231(ndhF) MTU: Rv3145(nuoA) Rv3146(nuoB) Rv3147(nuoC) Rv3148(nuoD) Rv3149(nuoE) Rv3150(nuoF) Rv3151(nuoG) Rv3152(nuoH) Rv3153(nuoI) Rv3154(nuoJ) Rv3155(nuoK) Rv3156(nuoL) Rv3157(nuoM) Rv3158(nuoN) SYN: sll0026(ndhF) sll0027(ndhD4) sll0223(ndhB) sll0519(ndhA) sll0520(ndhI) sll0521(ndhG) sll0522(ndhE) sll1220 sll1221(hoxF) sll1223(hoxU) sll1732(ndhF) sll1733(ndhD3) slr0261(ndhH) slr0331(ndhD1) slr0844(ndhF) slr1279(ndhC) slr1280(psbg1) slr1281(ndhJ) slr1291(ndhD2) slr2007(ndhD5) slr2009(ndhD6) DRA: DR0880 DR1492 DR1493 DR1494 DR1495 DR1496 DR1497 DR1498 DR1499 DR1500 DR1501 DR1503 DR1504 DR1505 DR1506 AAE: aq_1310(nuoA2) aq_1312(nuoB) aq_1314(nuoD2) aq_1315(nuoH1) aq_1317(nuoI1) aq_1318(nuoJ1) aq_1319(nuoK1) aq_1320(nuoL1) aq_1321(nuoM1) aq_1322(nuoN1) aq_135(nueM) aq_1373(nuoH2) aq_1374(nuoH3) aq_1375(nuoI2) aq_1377(nuoJ2) aq_1378(nuoK2) aq_1379(nuoL3) aq_1382(nuoM2) aq_1383(nuoN2) aq_1385(nuoA1) aq_437(nuoG) aq_551(nuoD1) aq_573(nuoF) aq_574(nuoE) aq_866(nuoL2) TMA: TM0010 TM0011 TM0012 TM0201 TM0228 TM1105 TM1211 TM1212 TM1213 TM1214 TM1215 TM1216 TM1424 TM1425 TM1426 MJA: MJ0520 MJ1309(cooM) MJ1362 MTH: MTH1237 MTH1246 MTH1548 MTH1549 MTH393 AFU: AF1823 AF1824 AF1825(nuoM) AF1826(nuoL) AF1827 AF1828 AF1829 AF1830(nuoD) AF1831 HAL: VNG0635G(nolB) VNG0636G(ndhG1) VNG0637G(ndhG5) VNG0639G(ndhG4) VNG0640G(nolD) VNG0641C VNG0643G(nolC) VNG0646G(nuoL) VNG0647G(nuoM) VNG0648G(ndhG3) TAC: Ta0959 Ta0960 Ta0961 Ta0962 Ta0964 Ta0965 Ta0966 Ta0967 Ta0968 Ta0969 Ta0970 TVO: TVG1157047 TVG1158465 TVG1160017 TVG1161017 TVG1162018 TVG1162541 TVG1162786 TVG1163214 TVG1164251 TVG1165354 TVG1165809 TVG1166270 PHO: PH0941 PH0942 PH1431 PH1446 PH1447 PH1448 PH1449 PH1450 PH1451 PH1452 PH1453 PAB: PAB0488 PAB0490(nuoM) PAB0492(nuoH) PAB0493(nuoB) PAB0494(nuoC) PAB0495(nuoD) PAB0496(nuoI) PAB0805 PAB0806 PAB1888 PAB2416(nuoN) APE: APE1410 APE1411 APE1415 APE1417 APE1418 APE1419 APE1421 APE1422 APE1426 APE1428 APE1430 SSO: SSO0322(NuoA) SSO0323(NuoC) SSO0324(NuoD) SSO0325(NuoH) SSO0326(NuoI) SSO0327(NuoJ) SSO0328(NuoL) SSO0329(NuoN) SSO0665(NuoB) SCE: YKL192C(ACP1) SPO: SPAC11E3.12(spac11e3.12) SPAC4H3.09(spac4h3.09) SPBC18E5.10(spbc18e5.10) ATH: AT4g02580(T10P11_14) AT5g08530(MAH20_9) AT5g11770(T22P22_160) AT5g37510(mpa22_p_40) At1g16700(F19K19_1) At1g65290(T8F5_6) At1g79010(YUP8H12R_21) At1g79200(YUP8H12R_37) At2g02050(F14H20.12) At2g20360(F11A3.9) At2g44620(F16B22.11) CEL: C09H10.3 C16A3.4 C18E9.4 C25A1.13 C33A12.1 D2030.4 F16B4.6 F22D6.4 F37C12.3 F44G4.2 F53F4.10 F59C6.5 K04G7.4 K09A9.5(gas-1) T10E9.7 T20H4.5 T26A5.3 W10D5.2 Y51H1A.3a Y51H1A.3b Y57G11C.12 Y63D3A.7 DME: CG10320 CG11423 CG11913 CG12079 CG15328 CG15434 CG1970 CG2014 CG2286(ND75) CG3192 CG3621(EG:152A3.7) CG3683 CG3944 CG5548 CG5703 CG6020 CG6343(ND42) CG6463 CG6485 CG6914 CG7712 CG8102 CG8844 CG9140 CG9160(mtacp1) CG9160_1(mtacp1) CG9172 CG9306 CG9762 HSA: 4535(MTND1) 4536(MTND2) 4537(MTND3) 4538(MTND4) 4539(MTND4L) 4540(MTND5) 4541(MTND6) 4694(NDUFA1) 4695(NDUFA2) 4696(NDUFA3) 4697(NDUFA4) 4698(NDUFA5) 4700(NDUFA6) 4701(NDUFA7) 4702(NDUFA8) 4704(NDUFA9) 4705(NDUFA10) 4706(NDUFAB1) 4707(NDUFB1) 4708(NDUFB2) 4709(NDUFB3) 4710(NDUFB4) 4711(NDUFB5) 4712(NDUFB6) 4713(NDUFB7) 4714(NDUFB8) 4715(NDUFB9) 4716(NDUFB10) 4717(NDUFC1) 4718(NDUFC2) 4719(NDUFS1) 4720(NDUFS2) 4722(NDUFS3) 4723(NDUFV1) 4724(NDUFS4) 4725(NDUFS5) 4726(NDUFS6) 4727(NDUFS7) 4728(NDUFS8) 4729(NDUFV2) 4731(NDUFV3) DISEASE MIM: 300078 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kD MIM: 601677 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 (13kD, B13) DBLINKS IUBMB Enzyme Nomenclature: 1.6.5.3 ExPASy - ENZYME nomenclature database: 1.6.5.3 WIT (What Is There) Metabolic Reconstruction: 1.6.5.3 BRENDA, the Enzyme Database: 1.6.5.3 /// ENTRY EC 1.14.13.28 NAME 3,9-Dihydroxypterocarpan 6a-monooxygenase 3,9-Dihydroxypterocarpan 6a-hydroxylase CLASS Oxidoreductases Acting on paired donors with incorporation of molecular oxygen With NADH or NADPH as one donor, and incorporation of one atom of oxygen SYSNAME (6aR,11aR)-3,9-Dihydroxypterocarpan,NADPH:oxygen oxidoreductase $ (6a-hydroxylating) REACTION (6aR,11aR)-3,9-Dihydroxypterocarpan + NADPH + O2 = (6aS,11aS)-3,6a,9-Trihydroxypterocarpan + NADP+ + H2O SUBSTRATE (6aR,11aR)-3,9-Dihydroxypterocarpan NADPH O2 PRODUCT (6aS,11aS)-3,6a,9-Trihydroxypterocarpan NADP+ H2O COFACTOR Heme COMMENT Possibly a heme-thiolate protein (P-450). The product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean. DBLINKS IUBMB Enzyme Nomenclature: 1.14.13.28 ExPASy - ENZYME nomenclature database: 1.14.13.28 WIT (What Is There) Metabolic Reconstruction: 1.14.13.28 BRENDA, the Enzyme Database: 1.14.13.28 /// ENTRY EC 2.4.1.68 NAME Glycoprotein 6-alpha-L-fucosyltransferase GDPfucose--glycoprotein fucosyltransferase CLASS Transferases Glycosyltransferases Hexosyltransferases SYSNAME GDP-L-fucose:glycoprotein (L-fucose to asparagine-linked $ N-acetylglucosamine of N-acetyl-beta-D-glucosaminyl-1,2-alpha- $D-mannosyl-1,3-(R1-alpha-1,6)-beta-D-mannosyl-1,4-beta-N-acetyl- $D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl)asparagine 6-alpha-L- $fucosyltransferase REACTION GDP-L-fucose + N4-(N-Acetyl-beta-D-glucosaminyl-1,2-alpha-D- $mannosyl-1,3-(R1-alpha-1,6)-beta-D-mannosyl-beta-N-acetyl-1,4- $D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl)asparagine = GDP + N4-(N-Acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3- $(R1-alpha-1,6)-beta-D-mannosyl-1,4-beta-N-acetyl-D-glucosaminyl- $1,4-(alpha-L-fucosyl-1,6)-N-acetyl-D-glucosaminyl)asparagine SUBSTRATE GDP-L-fucose N4-(N-Acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3- $(R1-alpha-1,6)-beta-D-mannosyl-beta-N-acetyl-1,4-D-glucosaminyl- $1,4-N-acetyl-D-glucosaminyl)asparagine PRODUCT GDP N4-(N-Acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3- $(R1-alpha-1,6)-beta-D-mannosyl-1,4-beta-N-acetyl-D-glucosaminyl- $1,4-(alpha-L-fucosyl-1,6)-N-acetyl-D-glucosaminyl)asparagine PATHWAY PATH: MAP00510 Glycoprotein biosynthesis DBLINKS IUBMB Enzyme Nomenclature: 2.4.1.68 ExPASy - ENZYME nomenclature database: 2.4.1.68 WIT (What Is There) Metabolic Reconstruction: 2.4.1.68 BRENDA, the Enzyme Database: 2.4.1.68 /// ENTRY EC 3.1.1.6 NAME Acetylesterase C-esterase (in animal tissues) CLASS Hydrolases Acting on ester bonds Carboxylic ester hydrolases SYSNAME Acetic-ester acetylhydrolase REACTION an Acetic ester + H2O = an Alcohol + Acetate SUBSTRATE Acetic ester H2O PRODUCT Alcohol Acetate GENES MMU: 95428(Es17) STRUCTURES PDB: 1BS9 1G66 2AXE DBLINKS IUBMB Enzyme Nomenclature: 3.1.1.6 ExPASy - ENZYME nomenclature database: 3.1.1.6 WIT (What Is There) Metabolic Reconstruction: 3.1.1.6 UM-BBD (Biocatalysis/Biodegradation Database): 3.1.1.6 BRENDA, the Enzyme Database: 3.1.1.6 SCOP (Structural Classification of Proteins): 3.1.1.6 /// ENTRY EC 2.7.2.1 NAME Acetate kinase Acetokinase CLASS Transferases Transferring phosphorus-containing groups Phosphotransferases with a carboxyl group as acceptor SYSNAME ATP:acetate phosphotransferase REACTION ATP + Acetate = ADP + Acetyl phosphate SUBSTRATE ATP Acetate Propanoate PRODUCT ADP Acetyl phosphate Propanoyl phosphate COMMENT Propanoate also acts as acceptor, but more slowly. PATHWAY PATH: MAP00620 Pyruvate metabolism PATH: MAP00640 Propanoate metabolism GENES ECO: ECOLI_2245(ackA) HIN: HI1204(ackA) BSU: ackA MGE: MG357(ackA) MPN: G12_orf390(ackA) SYN: sll1299(ackA) DBLINKS University of Geneva ENZYME DATA BANK: 2.7.2.1 WIT (What Is There) Metabolic Reconstruction: 2.7.2.1 PIR: B49338 B49935 E64239 KIECAA ///