RJR REBASE Enz 2540 - I-CreII-CreI
                  Homing endonuclease 


            NOTE: Homing endonucleases do not really have recognition sequences 
            in the way that restriction enzymes do. The recognition sequence 
            listed is one site that is known to be recognized and cleaved. In 
            general, single base changes merely change the efficiency of 
            cleavage and the precise boundary of required bases is not known. 


      Recognition Sequence: 
 C T G G G T T C A A A A C G T C G T G A^G A C A G T T T G G 0  G A C C C A A G T T T T G C A G^C A C T C T G T C A A A C C 0 
                        REBASE enzyme #: 2540
                        Prototype: I-CreI
                        Source: J.-D. Rochaix
                        Microorganism: Chlamydomonas reinhardtii 
                        Growth Temperature: 26 °
                        Enzyme gene cloned.
                        Enzyme gene sequenced.
                        Crystal data present.
                        Comment: Insertion site CGT^CGT
                        Entered: Jan 1 1979 ... Modified: Sep 9 1999 


                  # sites on
                  Adeno2: 0
                  Lambda: 0
                  pBR322: 0
                  PhiX174: 0
                  SV40: 0 



            Related References...
            (sorted by date) (sorted by authors)NOT commercially 
            availableSequence Data... Crystal Data...

                  07/13/2000
                  Dr. Richard J. Roberts
                  and Dana Macelis