ID 1A02_HUMAN STANDARD; PRT; 365 AA. AC P01892; P06338; P30514; P30444; P30445; P30446; Q29680; Q29899; AC Q95352; Q29837; Q95380; DT 21-JUL-1986 (Rel. 01, Created) DT 13-AUG-1987 (Rel. 05, Last sequence update) DT 15-FEB-2000 (Rel. 39, Last annotation update) DE HLA CLASS I HISTOCOMPATIBILITY ANTIGEN, A-2 ALPHA CHAIN PRECURSOR. GN HLA-A OR HLAA. OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia; OC Eutheria; Primates; Catarrhini; Hominidae; Homo. RN [1] RP SEQUENCE FROM N.A. (A*0201). RX MEDLINE; 85132727. RA Koller B.H., Orr H.T.; RT "Cloning and complete sequence of an HLA-A2 gene: analysis of two RT HLA-A alleles at the nucleotide level."; RL J. Immunol. 134:2727-2733(1985). RN [2] RP SEQUENCE FROM N.A. (A*0201). RX MEDLINE; 89122144. RA Cianetti L., Testa U., Scotto L., la Valle R., Simeone A., RA Boccoli G., Giannella G., Peschle C., Boncinelli E.; RT "Three new class I HLA alleles: structure of mRNAs and alternative RT mechanisms of processing."; RL Immunogenetics 29:80-91(1989). RN [3] RP SEQUENCE FROM N.A. (A*0201). RX MEDLINE; 90207291. RA Ennis P.D., Zemmour J., Salter R.D., Parham P.; RT "Rapid cloning of HLA-A,B cDNA by using the polymerase chain RT reaction: frequency and nature of errors produced in amplification."; RL Proc. Natl. Acad. Sci. U.S.A. 87:2833-2837(1990). RN [4] RP SEQUENCE FROM N.A. (A*0201/A*0211/A*0212). RX MEDLINE; 92269955. RA Belich M.P., Madrigal J.A., Hildebrand W.H., Zemmour J., RA Williams R.C., Luz R., Petzl-Erler M.L., Parham P.; RT "Unusual HLA-B alleles in two tribes of Brazilian Indians."; RL Nature 357:326-329(1992). RN [5] RP SEQUENCE OF 39-365 FROM N.A. (A*0201). RX MEDLINE; 85230571. RA Krangel M.S.; RT "Unusual RNA splicing generates a secreted form of HLA-A2 in a RT mutagenized B lymphoblastoid cell line."; RL EMBO J. 4:1205-1210(1985). RN [6] RP SEQUENCE OF 25-295 (A*0201). RX MEDLINE; 80056745. RA Orr H.T., Lopez de Castro J.A., Parham P., Ploegh H.L., RA Strominger J.L.; RT "Comparison of amino acid sequences of two human histocompatibility RT antigens, HLA-A2 and HLA-B7: location of putative alloantigenic RT sites."; RL Proc. Natl. Acad. Sci. U.S.A. 76:4395-4399(1979). RN [7] RP REVISIONS (A*0201). RX MEDLINE; 82247941. RA Lopez de Castro J.A., Strominger J.L., Strong D.M., Orr H.T.; RT "Structure of crossreactive human histocompatibility antigens HLA-A28 RT and HLA-A2: possible implications for the generation of HLA RT polymorphism."; RL Proc. Natl. Acad. Sci. U.S.A. 79:3813-3817(1982). RN [8] RP SEQUENCE OF 26-298 FROM N.A. (A*0202/A*0203). RX MEDLINE; 87306734. RA Mattson D.H., Handy D.E., Bradley D.A., Coligan J.E., Cowan E.P., RA Biddison W.E.; RT "DNA sequences of the genes that encode the CTL-defined HLA-A2 RT variants M7 and DK1."; RL Immunogenetics 26:190-192(1987). RN [9] RP SEQUENCE FROM N.A. (A*0203/A*0205). RX MEDLINE; 87252273. RA Holmes N., Ennis P., Wan A.M., Denney D.W., Parham P.; RT "Multiple genetic mechanisms have contributed to the generation of RT the HLA-A2/A28 family of class I MHC molecules."; RL J. Immunol. 139:936-941(1987). RN [10] RP SEQUENCE FROM N.A. (A*0203/A*0205). RA Domena J.D.; RL Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases. RN [11] RP SEQUENCE OF 9-365 FROM N.A. (A*0204). RX MEDLINE; 92039809. RA Castano A.R., Lopez de Castro J.A.; RT "Structure of the HLA-A*0204 antigen, found in South American RT Indians. Spatial clustering of HLA-A2 subtype polymorphism."; RL Immunogenetics 34:281-285(1991). RN [12] RP SEQUENCE OF 9-365 FROM N.A. (A*0204). RX MEDLINE; 92269956. RA Watkins D.I., McAdam S.N., Liu X., Stang C.R., Milford E.L., RA Levine C.G., Garber T.L., Dogon A.L., Lord C.I., Ghim S.H., RA Troup G.M., Hughes A.L., Letvin N.L.; RT "New recombinant HLA-B alleles in a tribe of South American RT Amerindians indicate rapid evolution of MHC class I loci."; RL Nature 357:329-333(1992). RN [13] RP SEQUENCE FROM N.A. (A*0206). RX MEDLINE; 89235215. RA Parham P., Lawlor D.A., Lomen C.E., Ennis P.D.; RT "Diversity and diversification of HLA-A,B,C alleles."; RL J. Immunol. 142:3937-3950(1989). RN [14] RP PARTIAL SEQUENCE (A*0206). RX MEDLINE; 86305811. RA Ezquerra A., Domenech N., van der Poel J., Strominger J.L., Vega M.A., RA Lopez de Castro J.A.; RT "Molecular analysis of an HLA-A2 functional variant CLA defined by RT cytolytic T lymphocytes."; RL J. Immunol. 137:1642-1649(1986). RN [15] RP PARTIAL SEQUENCE (A*0207). RX MEDLINE; 88113844. RA Domenech N., Ezquerra A., Castano R., Lopez de Castro J.A.; RT "Structural analysis of HLA-A2.4 functional variant KNE. Implications RT for the mapping of HLA-A2-specific T-cell epitopes."; RL Immunogenetics 27:196-202(1988). RN [16] RP PARTIAL SEQUENCE (A*0208). RX MEDLINE; 88314183. RA Domenech N., Castano R., Goulmy E., Lopez de Castro J.A.; RT "Molecular analysis of HLA-A2.4 functional variant KLO: close RT structural and evolutionary relatedness to the HLA-A2.2 subtype."; RL Immunogenetics 28:143-152(1988). RN [17] RP PARTIAL SEQUENCE (A*0209). RX MEDLINE; 88186100. RA Castano R., Ezquerra A., Domenech N., Lopez de Castro J.A.; RT "An HLA-A2 population variant with structural polymorphism in the RT alpha 3 region."; RL Immunogenetics 27:345-355(1988). RN [18] RP SEQUENCE FROM N.A. (A*0210). RX MEDLINE; 89122133. RA Epstein H., Kennedy L., Holmes N.; RT "An Oriental HLA-A2 subtype is closely related to a subset of RT Caucasoid HLA-A2 alleles."; RL Immunogenetics 29:112-116(1989). RN [19] RP SEQUENCE OF 9-365 FROM N.A. (A*0211). RX MEDLINE; 92218010. RA Castano A.R., Lopez de Castro J.A.; RT "Structure of the HLA-A*0211 (A2.5) subtype: further evidence for RT selection-driven diversification of HLA-A2 antigens."; RL Immunogenetics 35:344-346(1992). RN [20] RP SEQUENCE FROM N.A. (A*0213). RX MEDLINE; 94222455. RA Barber D.F., Fernandez J.M., Lopez de Castro J.A.; RT "Primary structure of a new HLA-A2 subtype: HLA-A*0213."; RL Immunogenetics 39:378-378(1994). RN [21] RP SEQUENCE FROM N.A. (A*0216). RX MEDLINE; 95278976. RA Barouch D., Krausa P., Bodmer J., Browning M.J., McMichael A.J.; RT "Identification of a novel HLA-A2 subtype, HLA-A*0216."; RL Immunogenetics 41:388-388(1995). RN [22] RP SEQUENCE FROM N.A. (A*0217). RC TISSUE=BLOOD; RX MEDLINE; 95381236. RA Selvakumar A., Granja C.B., Salazar M., Alosco S.M., Yunis E.J., RA Dupont B.; RT "A novel subtype of A2 (A*0217) isolated from the South American RT Indian B-cell line AMALA."; RL Tissue Antigens 45:343-347(1995). RN [23] RP SEQUENCE FROM N.A. (A*0218). RC TISSUE=BLOOD; RA Kashiwase K., Tokunaga K., Ishikawa Y., Oohashi H., Hashimoto M., RA Akaza T., Tadokoro K., Juji T.; RT "A new A2 sequence HLA-A2K from Japanese."; RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases. RN [24] RP SEQUENCE FROM N.A. (A*0220). RC TISSUE=BLOOD; RX MEDLINE; 97161038. RA Fleischhauer K., Zino E., Mazzi B., Severini G.M., Benazzi E., RA Bordignon C.; RT "HLA-A*02 subtype distribution in Caucasians from northern Italy: RT identification of A*0220."; RL Tissue Antigens 48:673-679(1996). RN [25] RP SEQUENCE FROM N.A. (A*0221). RC TISSUE=BLOOD; RA Szmania S., Baxter-Lowe L.A.; RT "Nucleotide sequence of a novel HLA-A2 gene."; RL Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases. RN [26] RP X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF A*0201. RX MEDLINE; 88014204. RA Bjorkman P.J., Saper M.A., Samraoui B., Bennett W.S., RA Strominger J.L., Wiley D.C.; RT "Structure of the human class I histocompatibility antigen, HLA-A2."; RL Nature 329:506-512(1987). RN [27] RP X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF A*0201. RX MEDLINE; 91245570. RA Saper M.A., Bjorkman P.J., Wiley D.C.; RT "Refined structure of the human histocompatibility antigen HLA-A2 at RT 2.6-A resolution."; RL J. Mol. Biol. 219:277-319(1991). CC -!- FUNCTION: INVOLVED IN THE PRESENTATION OF FOREIGN ANTIGENS TO CC THE IMMUNE SYSTEM. CC -!- SUBUNIT: DIMER OF ALPHA CHAIN AND A BETA CHAIN (BETA-2- CC MICROGLOBULIN). CC -!- POLYMORPHISM: THE FOLLOWING ALLELES OF A-2 ARE KNOWN: A*0201, CC A*0202, A*0203, A*0204, A*0205, A*0206 (A2.4A), A*0207, A*0208, CC A*0209, A*0210, A*0211 (A2.5), A*0212, A*0213 (A*02SLU), A*0216, CC A*0217, A*0218 (A2K), A*0219, A*0220 AND A*0221. THE SEQUENCE CC SHOWN IS THAT OF A*0201. CC -!- CAUTION: REF.6 AND REF.7 SEQUENCES DIFFER FROM THAT SHOWN CC EXTENSIVELY. CC -------------------------------------------------------------------------- CC This SWISS-PROT entry is copyright. It is produced through a collaboration CC between the Swiss Institute of Bioinformatics and the EMBL outstation - CC the European Bioinformatics Institute. There are no restrictions on its CC use by non-profit institutions as long as its content is in no way CC modified and this statement is not removed. Usage by and for commercial CC entities requires a license agreement (See http://www.isb-sib.ch/announce/ CC or send an email to license@isb-sib.ch). CC -------------------------------------------------------------------------- DR EMBL; K02883; AAA98727.1; -. DR EMBL; M84379; AAA59606.1; -. DR EMBL; X02457; CAA26297.1; -. DR EMBL; M19670; AAA03683.1; -. DR EMBL; M17690; AAB02120.1; -. DR EMBL; U03863; AAA03604.1; -. DR EMBL; M86404; -; NOT_ANNOTATED_CDS. DR EMBL; X57954; CAA41022.1; ALT_SEQ. DR EMBL; U03862; AAA03603.1; -. DR EMBL; M24042; AAA59653.1; -. DR EMBL; Z23071; CAA80612.1; -. DR EMBL; M84377; AAA59603.1; -. DR EMBL; X60764; -; NOT_ANNOTATED_CDS. DR EMBL; M84378; AAA59604.1; -. DR EMBL; Z27120; CAA81644.1; -. DR EMBL; Z46633; CAA86602.1; -. DR EMBL; U18930; AAA87076.1; -. DR EMBL; D83515; BAA11935.1; -. DR EMBL; X96724; CAA65501.1; -. DR EMBL; U56825; AAB17465.1; -. DR PIR; A02188; HLHUA2. DR PIR; A02191; HLHU2A. DR PIR; E35997; E35997. DR PIR; S19020; S19020. DR PIR; S23593; S23593. DR PDB; 1HLA; 15-JUL-90. DR PDB; 3HLA; 15-APR-90. DR PDB; 1HHG; 31-OCT-93. DR PDB; 1HHH; 31-OCT-93. DR PDB; 1HHI; 31-OCT-93. DR PDB; 1HHJ; 31-OCT-93. DR PDB; 1HHK; 31-OCT-93. DR PDB; 1AQD; 28-JAN-98. DR MIM; 142800; -. DR PROSITE; PS00290; IG_MHC; 1. DR PFAM; PF00047; ig; 1. DR PFAM; PF00129; MHC_I; 1. KW MHC I; Transmembrane; Glycoprotein; Signal; Polymorphism; KW 3D-structure. FT SIGNAL 1 24 FT CHAIN 25 365 HLA CLASS I HISTOCOMPATIBILITY ANTIGEN, FT A-2 ALPHA CHAIN. FT DOMAIN 25 114 EXTRACELLULAR ALPHA-1. FT DOMAIN 115 206 EXTRACELLULAR ALPHA-2. FT DOMAIN 207 298 EXTRACELLULAR ALPHA-3. FT DOMAIN 299 308 CONNECTING PEPTIDE. FT TRANSMEM 309 332 FT DOMAIN 333 365 CYTOPLASMIC TAIL. FT CARBOHYD 110 110 FT DISULFID 125 188 FT DISULFID 227 283 FT STRAND 27 36 FT STRAND 45 52 FT TURN 53 54 FT STRAND 55 61 FT TURN 62 63 FT STRAND 70 71 FT HELIX 74 76 FT TURN 77 78 FT HELIX 81 108 FT TURN 109 110 FT TURN 113 114 FT STRAND 118 127 FT TURN 129 130 FT STRAND 133 142 FT TURN 143 144 FT STRAND 145 150 FT TURN 152 153 FT STRAND 157 159 FT TURN 163 163 FT HELIX 164 173 FT TURN 174 175 FT HELIX 176 185 FT TURN 186 186 FT HELIX 187 198 FT TURN 199 199 FT HELIX 200 203 FT TURN 204 204 FT STRAND 207 207 FT STRAND 210 219 FT TURN 220 221 FT STRAND 222 233 FT STRAND 238 243 FT TURN 244 245 FT STRAND 246 247 FT HELIX 249 251 FT STRAND 253 254 FT STRAND 258 259 FT STRAND 265 274 FT TURN 275 276 FT HELIX 278 280 FT STRAND 281 286 FT TURN 288 289 FT STRAND 294 297 FT VARIANT 33 33 F -> Y (IN A*0205, A*0206, A*0208, A*0210 FT AND A*0221). FT /FTId=VAR_004334. FT VARIANT 54 54 D -> N (IN A*0221). FT /FTId=VAR_004335. FT VARIANT 67 67 Q -> R (IN A*0202, A*0205, AND A*0208). FT /FTId=VAR_004336. FT VARIANT 90 90 K -> N (IN A*0208 AND A*0220). FT /FTId=VAR_004337. FT VARIANT 97 98 TH -> ID (IN A*0211). FT /FTId=VAR_004338. FT VARIANT 119 119 V -> L (IN A*0202, A*0205, A*0208 AND FT A*0217). FT /FTId=VAR_004339. FT VARIANT 121 121 R -> M (IN A*0204 AND A*0217). FT /FTId=VAR_004340. FT VARIANT 123 123 Y -> C (IN A*0207 AND A*0218). FT /FTId=VAR_004341. FT VARIANT 123 123 Y -> F (IN A*0210 AND A*0217). FT /FTId=VAR_004342. FT VARIANT 131 131 W -> G (IN A*0210). FT /FTId=VAR_004343. FT VARIANT 162 162 M -> K (IN A*0218). FT /FTId=VAR_004344. FT VARIANT 173 173 A -> T (IN A*0203). FT /FTId=VAR_004345. FT VARIANT 176 176 V -> E (IN A*0203 AND A*0213). FT /FTId=VAR_004346. FT VARIANT 180 180 L -> W (IN A*0202, A*0203, A*0205 AND FT A*0208). FT /FTId=VAR_004347. FT VARIANT 180 180 L -> Q (IN A*0212 AND A*0213). FT /FTId=VAR_004348. FT VARIANT 187 187 T -> E (IN A*0216). FT /FTId=VAR_004349. FT VARIANT 260 260 A -> E (IN A*0209). FT /FTId=VAR_004350. SQ SEQUENCE 365 AA; 40922 MW; B54A97B24B337C08 CRC64; MAVMAPRTLV LLLSGALALT QTWAGSHSMR YFFTSVSRPG RGEPRFIAVG YVDDTQFVRF DSDAASQRME PRAPWIEQEG PEYWDGETRK VKAHSQTHRV DLGTLRGYYN QSEAGSHTVQ RMYGCDVGSD WRFLRGYHQY AYDGKDYIAL KEDLRSWTAA DMAAQTTKHK WEAAHVAEQL RAYLEGTCVE WLRRYLENGK ETLQRTDAPK THMTHHAVSD HEATLRCWAL SFYPAEITLT WQRDGEDQTQ DTELVETRPA GDGTFQKWAA VVVPSGQEQR YTCHVQHEGL PKPLTLRWEP SSQPTIPIVG IIAGLVLFGA VITGAVVAAV MWRRKSSDRK GGSYSQAASS DSAQGSDVSL TACKV //