# Copyright 2001 Brad Chapman. # Revisions copyright 2009-2010 by Peter Cock. # Revisions copyright 2010 by Phillip Garland. # All rights reserved. # This code is part of the Biopython distribution and governed by its # license. Please see the LICENSE file that should have been included # as part of this package. """Definitions for interacting with BLAST related applications. Wrappers for the new NCBI BLAST+ tools (written in C++): - NcbiblastpCommandline - Protein-Protein BLAST - NcbiblastnCommandline - Nucleotide-Nucleotide BLAST - NcbiblastxCommandline - Translated Query-Protein Subject BLAST - NcbitblastnCommandline - Protein Query-Translated Subject BLAST - NcbitblastxCommandline - Translated Query-Protein Subject BLAST - NcbipsiblastCommandline - Position-Specific Initiated BLAST - NcbirpsblastCommandline - Reverse Position Specific BLAST - NcbirpstblastnCommandline - Translated Reverse Position Specific BLAST - NcbideltablastCommandline - Protein-Protein domain enhanced lookup time accelerated blast - NcbiblastformatterCommandline - Convert ASN.1 to other BLAST output formats For further details, see: Camacho et al. BLAST+: architecture and applications BMC Bioinformatics 2009, 10:421 doi:10.1186/1471-2105-10-421 """ from __future__ import print_function from Bio.Application import _Option, AbstractCommandline, _Switch class _NcbibaseblastCommandline(AbstractCommandline): """Base Commandline object for (new) NCBI BLAST+ wrappers (PRIVATE). This is provided for subclassing, it deals with shared options common to all the BLAST tools (blastn, rpsblast, rpsblast, etc AND blast_formatter). """ def __init__(self, cmd=None, **kwargs): assert cmd is not None extra_parameters = [ #Core: _Switch(["-h", "h"], "Print USAGE and DESCRIPTION; ignore other arguments."), _Switch(["-help", "help"], "Print USAGE, DESCRIPTION and ARGUMENTS description; " "ignore other arguments."), _Switch(["-version", "version"], "Print version number; ignore other arguments."), # Output configuration options _Option(["-out", "out"], "Output file for alignment.", filename=True, equate=False), #Formatting options: _Option(["-outfmt", "outfmt"], "Alignment view. Integer 0-11. Use 5 for XML output " "(differs from classic BLAST which used 7 for XML).", equate=False), #TODO - Document and test the column options _Switch(["-show_gis", "show_gis"], "Show NCBI GIs in deflines?"), _Option(["-num_descriptions", "num_descriptions"], """Number of database sequences to show one-line descriptions for. Integer argument (at least zero). Default is 500. See also num_alignments.""", equate=False), _Option(["-num_alignments", "num_alignments"], """Number of database sequences to show num_alignments for. Integer argument (at least zero). Default is 200. See also num_alignments.""", equate=False), _Switch(["-html", "html"], "Produce HTML output? See also the outfmt option."), #Miscellaneous options _Switch(["-parse_deflines", "parse_deflines"], "Should the query and subject defline(s) be parsed?"), ] try: #Insert extra parameters - at the start just in case there #are any arguments which must come last: self.parameters = extra_parameters + self.parameters except AttributeError: #Should we raise an error? The subclass should have set this up! self.parameters = extra_parameters AbstractCommandline.__init__(self, cmd, **kwargs) def _validate_incompatibilities(self, incompatibles): """Used by the BLAST+ _validate method (PRIVATE).""" for a in incompatibles: if self._get_parameter(a): for b in incompatibles[a]: if self._get_parameter(b): raise ValueError("Options %s and %s are incompatible." % (a, b)) class _NcbiblastCommandline(_NcbibaseblastCommandline): """Base Commandline object for (new) NCBI BLAST+ wrappers (PRIVATE). This is provided for subclassing, it deals with shared options common to all the BLAST tools (blastn, rpsblast, rpsblast, etc). """ def __init__(self, cmd=None, **kwargs): assert cmd is not None extra_parameters = [ #Input query options: _Option(["-query", "query"], "The sequence to search with.", filename=True, equate=False), # Should this be required? _Option(["-query_loc", "query_loc"], "Location on the query sequence (Format: start-stop)", equate=False), #General search options: _Option(["-db", "db"], "The database to BLAST against.", equate=False), _Option(["-evalue", "evalue"], "Expectation value cutoff.", equate=False), _Option(["-word_size", "word_size"], """Word size for wordfinder algorithm. Integer. Minimum 2.""", equate=False), #BLAST-2-Sequences options: # - see subclass #Formatting options: # - see baseclass #Query filtering options # TODO -soft_masking , is this a switch or an option? #_Switch(["-soft_masking", "soft_masking"], # "Apply filtering locations as soft masks?"), _Switch(["-lcase_masking", "lcase_masking"], "Use lower case filtering in query and subject sequence(s)?"), #Restrict search or results _Option(["-gilist", "gilist"], """Restrict search of database to list of GI's. Incompatible with: negative_gilist, seqidlist, remote, subject, subject_loc""", filename=True, equate=False), _Option(["-negative_gilist", "negative_gilist"], """Restrict search of database to everything except the listed GIs. Incompatible with: gilist, seqidlist, remote, subject, subject_loc""", filename=True, equate=False), _Option(["-seqidlist", "seqidlist"], """Restrict search of database to list of SeqID's. Incompatible with: gilist, negative_gilist, remote, subject, subject_loc""", filename=True, equate=False), _Option(["-entrez_query", "entrez_query"], "Restrict search with the given Entrez query (requires remote).", equate=False), _Option(["-max_target_seqs", "max_target_seqs"], "Maximum number of aligned sequences to keep (integer, at least one).", equate=False), #Statistical options _Option(["-dbsize", "dbsize"], "Effective length of the database (integer).", equate=False), _Option(["-searchsp", "searchsp"], "Effective length of the search space (integer).", equate=False), _Option(["-max_hsps_per_subject", "max_hsps_per_subject"], "Override maximum number of HSPs per subject to save for ungapped searches (integer).", equate=False), _Option(["-max_hsps", "max_hsps"], "Set maximum number of HSPs per subject sequence to save (default 0 means no limit).", equate=False), _Switch(["-sum_statistics", "sum_statistics"], "Use sum statistics."), #Extension options _Option(["-xdrop_ungap", "xdrop_ungap"], "X-dropoff value (in bits) for ungapped extensions (float).", equate=False), _Option(["-xdrop_gap", "xdrop_gap"], "X-dropoff value (in bits) for preliminary gapped extensions (float).", equate=False), _Option(["-xdrop_gap_final", "xdrop_gap_final"], "X-dropoff value (in bits) for final gapped alignment (float).", equate=False), _Option(["-window_size", "window_size"], "Multiple hits window size, use 0 to specify 1-hit algorithm (integer).", equate=False), # Search strategy options _Option(["-import_search_strategy", "import_search_strategy"], """Search strategy to use. Incompatible with: export_search_strategy""", filename=True, equate=False), _Option(["-export_search_strategy", "export_search_strategy"], """File name to record the search strategy used. Incompatible with: import_search_strategy""", filename=True, equate=False), #Miscellaneous options _Option(["-num_threads", "num_threads"], """Number of threads to use in the BLAST search (integer, at least one). Default is one. Incompatible with: remote""", equate=False), _Switch(["-remote", "remote"], """Execute search remotely? Incompatible with: gilist, negative_gilist, subject_loc, num_threads, ..."""), ] try: #Insert extra parameters - at the start just in case there #are any arguments which must come last: self.parameters = extra_parameters + self.parameters except AttributeError: #Should we raise an error? The subclass should have set this up! self.parameters = extra_parameters _NcbibaseblastCommandline.__init__(self, cmd, **kwargs) def _validate(self): incompatibles = {"remote":["gilist", "negative_gilist", "num_threads"], "import_search_strategy" : ["export_search_strategy"], "gilist":["negative_gilist"], "seqidlist":["gilist", "negative_gilist", "remote"]} self._validate_incompatibilities(incompatibles) if self.entrez_query and not self.remote : raise ValueError("Option entrez_query requires remote option.") AbstractCommandline._validate(self) class _Ncbiblast2SeqCommandline(_NcbiblastCommandline): """Base Commandline object for (new) NCBI BLAST+ wrappers (PRIVATE). This is provided for subclassing, it deals with shared options common to all the BLAST tools supporting two-sequence BLAST (blastn, psiblast, etc) but not rpsblast or rpstblastn. """ def __init__(self, cmd=None, **kwargs): assert cmd is not None extra_parameters = [ #General search options: _Option(["-gapopen", "gapopen"], "Cost to open a gap (integer).", equate=False), _Option(["-gapextend", "gapextend"], "Cost to extend a gap (integer).", equate=False), #BLAST-2-Sequences options: _Option(["-subject", "subject"], """Subject sequence(s) to search. Incompatible with: db, gilist, negative_gilist. See also subject_loc.""", filename=True, equate=False), _Option(["-subject_loc", "subject_loc"], """Location on the subject sequence (Format: start-stop). Incompatible with: db, gilist, seqidlist, negative_gilist, db_soft_mask, db_hard_mask, remote. See also subject.""", equate=False), #Restrict search or results: _Option(["-culling_limit", "culling_limit"], """Hit culling limit (integer). If the query range of a hit is enveloped by that of at least this many higher-scoring hits, delete the hit. Incompatible with: best_hit_overhang, best_hit_score_edge. """, equate=False), _Option(["-best_hit_overhang", "best_hit_overhang"], """Best Hit algorithm overhang value (float, recommended value: 0.1) Float between 0.0 and 0.5 inclusive. Incompatible with: culling_limit.""", equate=False), _Option(["-best_hit_score_edge", "best_hit_score_edge"], """Best Hit algorithm score edge value (float, recommended value: 0.1) Float between 0.0 and 0.5 inclusive. Incompatible with: culling_limit.""", equate=False), ] try: #Insert extra parameters - at the start just in case there #are any arguments which must come last: self.parameters = extra_parameters + self.parameters except AttributeError: #Should we raise an error? The subclass should have set this up! self.parameters = extra_parameters _NcbiblastCommandline.__init__(self, cmd, **kwargs) def _validate(self): incompatibles = {"subject_loc":["db", "gilist", "negative_gilist", "seqidlist", "remote"], "culling_limit":["best_hit_overhang", "best_hit_score_edge"], "subject":["db", "gilist", "negative_gilist", "seqidlist"]} self._validate_incompatibilities(incompatibles) _NcbiblastCommandline._validate(self) class _NcbiblastMain2SeqCommandline(_Ncbiblast2SeqCommandline): """Base Commandline object for (new) NCBI BLAST+ wrappers (PRIVATE). This is provided for subclassing, it deals with shared options common to the main BLAST tools blastp, blastn, blastx, tblastx, tblastn but not psiblast, rpsblast or rpstblastn. """ def __init__(self, cmd=None, **kwargs): assert cmd is not None extra_parameters = [ #Restrict search or results: _Option(["-db_soft_mask", "db_soft_mask"], """Filtering algorithm for soft masking (integer). Filtering algorithm ID to apply to the BLAST database as soft masking. Incompatible with: db_hard_mask, subject, subject_loc""", equate=False), _Option(["-db_hard_mask", "db_hard_mask"], """Filtering algorithm for hard masking (integer). Filtering algorithm ID to apply to the BLAST database as hard masking. Incompatible with: db_soft_mask, subject, subject_loc""", equate=False), ] try: #Insert extra parameters - at the start just in case there #are any arguments which must come last: self.parameters = extra_parameters + self.parameters except AttributeError: #Should we raise an error? The subclass should have set this up! self.parameters = extra_parameters _Ncbiblast2SeqCommandline.__init__(self, cmd, **kwargs) def _validate(self): incompatibles = {"db_soft_mask":["db_hard_mask", "subject", "subject_loc"], "db_hard_mask":["db_soft_mask", "subject", "subject_loc"]} self._validate_incompatibilities(incompatibles) _Ncbiblast2SeqCommandline._validate(self) class NcbiblastpCommandline(_NcbiblastMain2SeqCommandline): """Create a commandline for the NCBI BLAST+ program blastp (for proteins). With the release of BLAST+ (BLAST rewritten in C++ instead of C), the NCBI replaced the old blastall tool with separate tools for each of the searches. This wrapper therefore replaces BlastallCommandline with option -p blastp. >>> from Bio.Blast.Applications import NcbiblastpCommandline >>> cline = NcbiblastpCommandline(query="rosemary.pro", db="nr", ... evalue=0.001, remote=True, ungapped=True) >>> cline NcbiblastpCommandline(cmd='blastp', query='rosemary.pro', db='nr', evalue=0.001, remote=True, ungapped=True) >>> print(cline) blastp -query rosemary.pro -db nr -evalue 0.001 -remote -ungapped You would typically run the command line with cline() or via the Python subprocess module, as described in the Biopython tutorial. """ def __init__(self, cmd="blastp", **kwargs): self.parameters = [ #General search options: _Option(["-task", "task"], "Task to execute (string, blastp (default) or blastp-short).", checker_function=lambda value : value in ["blastp", "blastp-short"], equate=False), _Option(["-matrix", "matrix"], "Scoring matrix name (default BLOSUM62)."), _Option(["-threshold", "threshold"], "Minimum score for words to be added to the BLAST lookup table (float).", equate=False), _Option(["-comp_based_stats", "comp_based_stats"], """Use composition-based statistics (string, default 2, i.e. True). 0, F or f: no composition-based statistics 2, T or t, D or d : Composition-based score adjustment as in Bioinformatics 21:902-911, 2005, conditioned on sequence properties Note that tblastn also supports values of 1 and 3.""", checker_function=lambda value : value in "0Ft2TtDd", equate=False), #Query filtering options: _Option(["-seg", "seg"], """Filter query sequence with SEG (string). Format: "yes", "window locut hicut", or "no" to disable. Default is "12 2.2 2.5""", equate=False), #Extension options: _Switch(["-ungapped", "ungapped"], "Perform ungapped alignment only?"), #Miscellaneous options: _Switch(["-use_sw_tback", "use_sw_tback"], "Compute locally optimal Smith-Waterman alignments?"), ] _NcbiblastMain2SeqCommandline.__init__(self, cmd, **kwargs) class NcbiblastnCommandline(_NcbiblastMain2SeqCommandline): """Wrapper for the NCBI BLAST+ program blastn (for nucleotides). With the release of BLAST+ (BLAST rewritten in C++ instead of C), the NCBI replaced the old blastall tool with separate tools for each of the searches. This wrapper therefore replaces BlastallCommandline with option -p blastn. For example, to run a search against the "nt" nucleotide database using the FASTA nucleotide file "m_code.fasta" as the query, with an expectation value cut off of 0.001, saving the output to a file in XML format: >>> from Bio.Blast.Applications import NcbiblastnCommandline >>> cline = NcbiblastnCommandline(query="m_cold.fasta", db="nt", strand="plus", ... evalue=0.001, out="m_cold.xml", outfmt=5) >>> cline NcbiblastnCommandline(cmd='blastn', out='m_cold.xml', outfmt=5, query='m_cold.fasta', db='nt', evalue=0.001, strand='plus') >>> print(cline) blastn -out m_cold.xml -outfmt 5 -query m_cold.fasta -db nt -evalue 0.001 -strand plus You would typically run the command line with cline() or via the Python subprocess module, as described in the Biopython tutorial. """ def __init__(self, cmd="blastn", **kwargs): self.parameters = [ #Input query options: _Option(["-strand", "strand"], """Query strand(s) to search against database/subject. Values allowed are "both" (default), "minus", "plus".""", checker_function=lambda value : value in ["both", "minus", "plus"], equate=False), #General search options: _Option(["-task", "task"], """Task to execute (string, default 'megablast') Allowed values 'blastn', 'blastn-short', 'dc-megablast', 'megablast' (the default), or 'vecscreen'.""", checker_function=lambda value : value in ['blastn', 'blastn-short', 'dc-megablast', 'megablast', 'vecscreen'], equate=False), _Option(["-penalty", "penalty"], "Penalty for a nucleotide mismatch (integer, at most zero).", equate=False), _Option(["-reward", "reward"], "Reward for a nucleotide match (integer, at least zero).", equate=False), #TODO - Does this need an argument or is it a switch? #_Option(["-use_index", "use_index"], # "Use MegaBLAST database index (boolean).", # equate=False), _Option(["-index_name", "index_name"], "MegaBLAST database index name.", equate=False), #Query filtering options: _Option(["-dust", "dust"], """Filter query sequence with DUST (string). Format: 'yes', 'level window linker', or 'no' to disable. Default = '20 64 1'. """, equate=False), _Option(["-filtering_db", "filtering_db"], "BLAST database containing filtering elements (i.e. repeats).", equate=False), _Option(["-window_masker_taxid", "window_masker_taxid"], "Enable WindowMasker filtering using a Taxonomic ID (integer).", equate=False), _Option(["-window_masker_db", "window_masker_db"], "Enable WindowMasker filtering using this repeats database (string).", equate=False), #Restrict search or results: _Option(["-perc_identity", "perc_identity"], "Percent identity (real, 0 to 100 inclusive).", equate=False), #Discontiguous MegaBLAST options _Option(["-template_type", "template_type"], """Discontiguous MegaBLAST template type (string). Allowed values: 'coding', 'coding_and_optimal' or 'optimal' Requires: template_length.""", checker_function=lambda value : value in ['coding', 'coding_and_optimal', 'optimal'], equate=False), _Option(["-template_length", "template_length"], """Discontiguous MegaBLAST template length (integer). Allowed values: 16, 18, 21 Requires: template_type.""", checker_function=lambda value : value in [16, 18, 21, '16', '18', '21'], equate=False), #Extension options: _Switch(["-no_greedy", "no_greedy"], "Use non-greedy dynamic programming extension"), _Option(["-min_raw_gapped_score", "min_raw_gapped_score"], "Minimum raw gapped score to keep an alignment in the " "preliminary gapped and traceback stages (integer).", equate=False), _Switch(["-ungapped", "ungapped"], "Perform ungapped alignment only?"), _Option(["-off_diagonal_range", "off_diagonal_range"], """Number of off-diagonals to search for the 2nd hit (integer). Expects a positive integer, or 0 (default) to turn off. Added in BLAST 2.2.23+ """, equate=False), ] _NcbiblastMain2SeqCommandline.__init__(self, cmd, **kwargs) def _validate(self): if (self.template_type and not self.template_length) \ or (self.template_length and not self.template_type) : raise ValueError("Options template_type and template_type require each other.") _NcbiblastMain2SeqCommandline._validate(self) class NcbiblastxCommandline(_NcbiblastMain2SeqCommandline): """Wrapper for the NCBI BLAST+ program blastx (nucleotide query, protein database). With the release of BLAST+ (BLAST rewritten in C++ instead of C), the NCBI replaced the old blastall tool with separate tools for each of the searches. This wrapper therefore replaces BlastallCommandline with option -p blastx. >>> from Bio.Blast.Applications import NcbiblastxCommandline >>> cline = NcbiblastxCommandline(query="m_cold.fasta", db="nr", evalue=0.001) >>> cline NcbiblastxCommandline(cmd='blastx', query='m_cold.fasta', db='nr', evalue=0.001) >>> print(cline) blastx -query m_cold.fasta -db nr -evalue 0.001 You would typically run the command line with cline() or via the Python subprocess module, as described in the Biopython tutorial. """ def __init__(self, cmd="blastx", **kwargs): self.parameters = [ #Input query options: _Option(["-strand", "strand"], """Query strand(s) to search against database/subject. Values allowed are "both" (default), "minus", "plus".""", checker_function=lambda value : value in ["both", "minus", "plus"], equate=False), #Input query options: _Option(["-query_gencode", "query_gencode"], "Genetic code to use to translate query (integer, default 1).", equate=False), #General search options: _Option(["-frame_shift_penalty", "frame_shift_penalty"], """Frame shift penalty (integer, at least 1, default ignored) (OBSOLETE). This was removed in BLAST 2.2.27+""", equate=False), _Option(["-max_intron_length", "max_intron_length"], """Maximum intron length (integer). Length of the largest intron allowed in a translated nucleotide sequence when linking multiple distinct alignments (a negative value disables linking). Default zero.""", equate=False), _Option(["-matrix", "matrix"], "Scoring matrix name (default BLOSUM62).", equate=False), _Option(["-threshold", "threshold"], "Minimum score for words to be added to the BLAST lookup table (float).", equate=False), _Option(["-comp_based_stats", "comp_based_stats"], """Use composition-based statistics for blastp, blastx, or tblastn: D or d: default (equivalent to 2 ) 0 or F or f: no composition-based statistics 1: Composition-based statistics as in NAR 29:2994-3005, 2001 2 or T or t : Composition-based score adjustment as in Bioinformatics 21:902-911, 2005, conditioned on sequence properties 3: Composition-based score adjustment as in Bioinformatics 21:902-911, 2005, unconditionally For programs other than tblastn, must either be absent or be D, F or 0 Default = `2' """, equate=False), #Query filtering options: _Option(["-seg", "seg"], """Filter query sequence with SEG (string). Format: "yes", "window locut hicut", or "no" to disable. Default is "12 2.2 2.5""", equate=False), #Extension options: _Switch(["-ungapped", "ungapped"], "Perform ungapped alignment only?"), _Switch(["-use_sw_tback", "use_sw_tback"], "Compute locally optimal Smith-Waterman alignments?"), ] _NcbiblastMain2SeqCommandline.__init__(self, cmd, **kwargs) class NcbitblastnCommandline(_NcbiblastMain2SeqCommandline): """Wrapper for the NCBI BLAST+ program tblastn. With the release of BLAST+ (BLAST rewritten in C++ instead of C), the NCBI replaced the old blastall tool with separate tools for each of the searches. This wrapper therefore replaces BlastallCommandline with option -p tblastn. >>> from Bio.Blast.Applications import NcbitblastnCommandline >>> cline = NcbitblastnCommandline(help=True) >>> cline NcbitblastnCommandline(cmd='tblastn', help=True) >>> print(cline) tblastn -help You would typically run the command line with cline() or via the Python subprocess module, as described in the Biopython tutorial. """ def __init__(self, cmd="tblastn", **kwargs): self.parameters = [ #General search options: _Option(["-db_gencode", "db_gencode"], "Genetic code to use to translate query (integer, default 1).", equate=False), _Option(["-frame_shift_penalty", "frame_shift_penalty"], """Frame shift penalty (integer, at least 1, default ignored) (OBSOLETE). This was removed in BLAST 2.2.27+""", equate=False), _Option(["-max_intron_length", "max_intron_length"], """Maximum intron length (integer). Length of the largest intron allowed in a translated nucleotide sequence when linking multiple distinct alignments (a negative value disables linking). Default zero.""", equate=False), _Option(["-matrix", "matrix"], "Scoring matrix name (default BLOSUM62).", equate=False), _Option(["-threshold", "threshold"], "Minimum score for words to be added to the BLAST lookup table (float).", equate=False), _Option(["-comp_based_stats", "comp_based_stats"], """Use composition-based statistics (string, default 2, i.e. True). 0, F or f: no composition-based statistics 1: Composition-based statistics as in NAR 29:2994-3005, 2001 2, T or t, D or d : Composition-based score adjustment as in Bioinformatics 21:902-911, 2005, conditioned on sequence properties 3: Composition-based score adjustment as in Bioinformatics 21:902-911, 2005, unconditionally Note that only tblastn supports values of 1 and 3.""", checker_function=lambda value : value in "0Ft12TtDd3", equate=False), #Query filtering options: _Option(["-seg", "seg"], """Filter query sequence with SEG (string). Format: "yes", "window locut hicut", or "no" to disable. Default is "12 2.2 2.5""", equate=False), #Extension options: _Switch(["-ungapped", "ungapped"], "Perform ungapped alignment only?"), #Miscellaneous options: _Switch(["-use_sw_tback", "use_sw_tback"], "Compute locally optimal Smith-Waterman alignments?"), #PSI-TBLASTN options: _Option(["-in_pssm", "in_pssm"], """PSI-BLAST checkpoint file Incompatible with: remote, query""", filename=True, equate=False), ] _NcbiblastMain2SeqCommandline.__init__(self, cmd, **kwargs) class NcbitblastxCommandline(_NcbiblastMain2SeqCommandline): """Wrapper for the NCBI BLAST+ program tblastx. With the release of BLAST+ (BLAST rewritten in C++ instead of C), the NCBI replaced the old blastall tool with separate tools for each of the searches. This wrapper therefore replaces BlastallCommandline with option -p tblastx. >>> from Bio.Blast.Applications import NcbitblastxCommandline >>> cline = NcbitblastxCommandline(help=True) >>> cline NcbitblastxCommandline(cmd='tblastx', help=True) >>> print(cline) tblastx -help You would typically run the command line with cline() or via the Python subprocess module, as described in the Biopython tutorial. """ def __init__(self, cmd="tblastx", **kwargs): self.parameters = [ #Input query options: _Option(["-strand", "strand"], """Query strand(s) to search against database/subject. Values allowed are "both" (default), "minus", "plus".""", checker_function=lambda value : value in ["both", "minus", "plus"], equate=False), #Input query options: _Option(["-query_gencode", "query_gencode"], "Genetic code to use to translate query (integer, default 1).", equate=False), #General search options: _Option(["-db_gencode", "db_gencode"], "Genetic code to use to translate query (integer, default 1).", equate=False), _Option(["-max_intron_length", "max_intron_length"], """Maximum intron length (integer). Length of the largest intron allowed in a translated nucleotide sequence when linking multiple distinct alignments (a negative value disables linking). Default zero.""", equate=False), _Option(["-matrix", "matrix"], "Scoring matrix name (default BLOSUM62).", equate=False), _Option(["-threshold", "threshold"], "Minimum score for words to be added to the BLAST lookup table (float).", equate=False), #Query filtering options: _Option(["-seg", "seg"], """Filter query sequence with SEG (string). Format: "yes", "window locut hicut", or "no" to disable. Default is "12 2.2 2.5""", equate=False), ] _NcbiblastMain2SeqCommandline.__init__(self, cmd, **kwargs) class NcbipsiblastCommandline(_Ncbiblast2SeqCommandline): """Wrapper for the NCBI BLAST+ program psiblast. With the release of BLAST+ (BLAST rewritten in C++ instead of C), the NCBI replaced the old blastpgp tool with a similar tool psiblast. This wrapper therefore replaces BlastpgpCommandline, the wrapper for blastpgp. >>> from Bio.Blast.Applications import NcbipsiblastCommandline >>> cline = NcbipsiblastCommandline(help=True) >>> cline NcbipsiblastCommandline(cmd='psiblast', help=True) >>> print(cline) psiblast -help You would typically run the command line with cline() or via the Python subprocess module, as described in the Biopython tutorial. """ def __init__(self, cmd="psiblast", **kwargs): self.parameters = [ #General search options: _Option(["-matrix", "matrix"], "Scoring matrix name (default BLOSUM62).", equate=False), _Option(["-threshold", "threshold"], "Minimum score for words to be added to the BLAST lookup table (float).", equate=False), _Option(["-comp_based_stats", "comp_based_stats"], """Use composition-based statistics (string, default 2, i.e. True). 0, F or f: no composition-based statistics 2, T or t, D or d : Composition-based score adjustment as in Bioinformatics 21:902-911, 2005, conditioned on sequence properties Note that tblastn also supports values of 1 and 3.""", checker_function=lambda value : value in "0Ft2TtDd", equate=False), #Query filtering options: _Option(["-seg", "seg"], """Filter query sequence with SEG (string). Format: "yes", "window locut hicut", or "no" to disable. Default is "12 2.2 2.5""", equate=False), #Extension options: _Option(["-gap_trigger", "gap_trigger"], "Number of bits to trigger gapping (float, default 22).", equate=False), #Miscellaneous options: _Switch(["-use_sw_tback", "use_sw_tback"], "Compute locally optimal Smith-Waterman alignments?"), #PSI-BLAST options: _Option(["-num_iterations", "num_iterations"], """Number of iterations to perform (integer, at least one). Default is one. Incompatible with: remote""", equate=False), _Option(["-out_pssm", "out_pssm"], "File name to store checkpoint file.", filename=True, equate=False), _Option(["-out_ascii_pssm", "out_ascii_pssm"], "File name to store ASCII version of PSSM.", filename=True, equate=False), _Option(["-in_msa", "in_msa"], """File name of multiple sequence alignment to restart PSI-BLAST. Incompatible with: in_pssm, query""", filename=True, equate=False), _Option(["-msa_master_idx", "msa_master_idx"], """Index of sequence to use as master in MSA. Index (1-based) of sequence to use as the master in the multiple sequence alignment. If not specified, the first sequence is used.""", equate=False), _Option(["-in_pssm", "in_pssm"], """PSI-BLAST checkpoint file. Incompatible with: in_msa, query, phi_pattern""", filename=True, equate=False), #PSSM engine options: _Option(["-pseudocount", "pseudocount"], """Pseudo-count value used when constructing PSSM. Integer. Default is zero.""", equate=False), _Option(["-inclusion_ethresh", "inclusion_ethresh"], "E-value inclusion threshold for pairwise alignments (float, default 0.002).", equate=False), _Switch(["-ignore_msa_master", "ignore_msa_master"], """Ignore the master sequence when creating PSSM Requires: in_msa Incompatible with: msa_master_idx, in_pssm, query, query_loc, phi_pattern """), #PHI-BLAST options: _Option(["-phi_pattern", "phi_pattern"], """File name containing pattern to search. Incompatible with: in_pssm""", filename=True, equate=False), ] _Ncbiblast2SeqCommandline.__init__(self, cmd, **kwargs) def _validate(self): incompatibles = {"num_iterations": ["remote"], "in_msa": ["in_pssm", "query"], "in_pssm": ["in_msa", "query", "phi_pattern"], "ignore_msa_master": ["msa_master_idx", "in_pssm", "query", "query_loc", "phi_pattern"], } self._validate_incompatibilities(incompatibles) _Ncbiblast2SeqCommandline._validate(self) class NcbirpsblastCommandline(_NcbiblastCommandline): """Wrapper for the NCBI BLAST+ program rpsblast. With the release of BLAST+ (BLAST rewritten in C++ instead of C), the NCBI replaced the old rpsblast tool with a similar tool of the same name. This wrapper replaces RpsBlastCommandline, the wrapper for the old rpsblast. >>> from Bio.Blast.Applications import NcbirpsblastCommandline >>> cline = NcbirpsblastCommandline(help=True) >>> cline NcbirpsblastCommandline(cmd='rpsblast', help=True) >>> print(cline) rpsblast -help You would typically run the command line with cline() or via the Python subprocess module, as described in the Biopython tutorial. """ def __init__(self, cmd="rpsblast", **kwargs): self.parameters = [ #Query filtering options: _Option(["-seg", "seg"], """Filter query sequence with SEG (string). Format: "yes", "window locut hicut", or "no" to disable. Default is "12 2.2 2.5""", equate=False), #Restrict search or results: _Option(["-culling_limit", "culling_limit"], """Hit culling limit (integer). If the query range of a hit is enveloped by that of at least this many higher-scoring hits, delete the hit. Incompatible with: best_hit_overhang, best_hit_score_edge. """, equate=False), _Option(["-best_hit_overhang", "best_hit_overhang"], """Best Hit algorithm overhang value (recommended value: 0.1) Float between 0.0 and 0.5 inclusive. Incompatible with: culling_limit.""", equate=False), _Option(["-best_hit_score_edge", "best_hit_score_edge"], """Best Hit algorithm score edge value (recommended value: 0.1) Float between 0.0 and 0.5 inclusive. Incompatible with: culling_limit.""", equate=False), #General search options: _Option(["-comp_based_stats", "comp_based_stats"], """Use composition-based statistics. D or d: default (equivalent to 0 ) 0 or F or f: Simplified Composition-based statistics as in Bioinformatics 15:1000-1011, 1999 1 or T or t: Composition-based statistics as in NAR 29:2994-3005, 2001 Default = `0' """, checker_function=lambda value : value in "Dd0Ff1Tt", equate=False), #Misc options: _Switch(["-use_sw_tback", "use_sw_tback"], "Compute locally optimal Smith-Waterman alignments?"), ] _NcbiblastCommandline.__init__(self, cmd, **kwargs) def _validate(self): incompatibles = {"culling_limit":["best_hit_overhang", "best_hit_score_edge"]} self._validate_incompatibilities(incompatibles) _NcbiblastCommandline._validate(self) class NcbirpstblastnCommandline(_NcbiblastCommandline): """Wrapper for the NCBI BLAST+ program rpstblastn. With the release of BLAST+ (BLAST rewritten in C++ instead of C), the NCBI replaced the old rpsblast tool with a similar tool of the same name, and a separate tool rpstblastn for Translated Reverse Position Specific BLAST. >>> from Bio.Blast.Applications import NcbirpstblastnCommandline >>> cline = NcbirpstblastnCommandline(help=True) >>> cline NcbirpstblastnCommandline(cmd='rpstblastn', help=True) >>> print(cline) rpstblastn -help You would typically run the command line with cline() or via the Python subprocess module, as described in the Biopython tutorial. """ def __init__(self, cmd="rpstblastn", **kwargs): self.parameters = [ #Input query options: _Option(["-strand", "strand"], """Query strand(s) to search against database/subject. Values allowed are "both" (default), "minus", "plus".""", checker_function=lambda value : value in ["both", "minus", "plus"], equate=False), #Input query options: _Option(["-query_gencode", "query_gencode"], "Genetic code to use to translate query (integer, default 1).", equate=False), #Query filtering options: _Option(["-seg", "seg"], """Filter query sequence with SEG (string). Format: "yes", "window locut hicut", or "no" to disable. Default is "12 2.2 2.5""", equate=False), #Extension options: _Switch(["-ungapped", "ungapped"], "Perform ungapped alignment only?"), ] _NcbiblastCommandline.__init__(self, cmd, **kwargs) class NcbiblastformatterCommandline(_NcbibaseblastCommandline): """Wrapper for the NCBI BLAST+ program blast_formatter. With the release of BLAST 2.2.24+ (i.e. the BLAST suite rewritten in C++ instead of C), the NCBI added the ASN.1 output format option to all the search tools, and extended the blast_formatter to support this as input. The blast_formatter command allows you to convert the ASN.1 output into the other output formats (XML, tabular, plain text, HTML). >>> from Bio.Blast.Applications import NcbiblastformatterCommandline >>> cline = NcbiblastformatterCommandline(archive="example.asn", outfmt=5, out="example.xml") >>> cline NcbiblastformatterCommandline(cmd='blast_formatter', out='example.xml', outfmt=5, archive='example.asn') >>> print(cline) blast_formatter -out example.xml -outfmt 5 -archive example.asn You would typically run the command line with cline() or via the Python subprocess module, as described in the Biopython tutorial. Note that this wrapper is for the version of blast_formatter from BLAST 2.2.24+ (or later) which is when the NCBI first announced the inclusion this tool. There was actually an early version in BLAST 2.2.23+ (and possibly in older releases) but this did not have the -archive option (instead -rid is a mandatory argument), and is not supported by this wrapper. """ def __init__(self, cmd="blast_formatter", **kwargs): self.parameters = [ # Input options _Option(["-rid", "rid"], "BLAST Request ID (RID), not compatible with archive arg", equate=False), _Option(["-archive", "archive"], "Archive file of results, not compatible with rid arg.", filename=True, equate=False), # Restrict search or results _Option(["-max_target_seqs", "max_target_seqs"], "Maximum number of aligned sequences to keep", checker_function=lambda value: value >= 1, equate=False), ] _NcbibaseblastCommandline.__init__(self, cmd, **kwargs) def _validate(self): incompatibles = {"rid":["archive"]} self._validate_incompatibilities(incompatibles) _NcbibaseblastCommandline._validate(self) class NcbideltablastCommandline(_Ncbiblast2SeqCommandline): """Create a commandline for the NCBI BLAST+ program deltablast (for proteins). This is a wrapper for the deltablast command line command included in the NCBI BLAST+ software (not present in the original BLAST). >>> from Bio.Blast.Applications import NcbideltablastCommandline >>> cline = NcbideltablastCommandline(query="rosemary.pro", db="nr", ... evalue=0.001, remote=True) >>> cline NcbideltablastCommandline(cmd='deltablast', query='rosemary.pro', db='nr', evalue=0.001, remote=True) >>> print(cline) deltablast -query rosemary.pro -db nr -evalue 0.001 -remote You would typically run the command line with cline() or via the Python subprocess module, as described in the Biopython tutorial. """ def __init__(self, cmd="deltablast", **kwargs): self.parameters = [ #General search options: _Option(["-matrix", "matrix"], "Scoring matrix name (default BLOSUM62)."), _Option(["-threshold", "threshold"], "Minimum score for words to be added to the BLAST lookup table (float).", equate=False), _Option(["-comp_based_stats", "comp_based_stats"], """Use composition-based statistics (string, default 2, i.e. True). 0, F or f: no composition-based statistics. 2, T or t, D or d : Composition-based score adjustment as in Bioinformatics 21:902-911, 2005, conditioned on sequence properties Note that tblastn also supports values of 1 and 3.""", checker_function=lambda value : value in "0Ft2TtDd", equate=False), #Query filtering options: _Option(["-seg", "seg"], """Filter query sequence with SEG (string). Format: "yes", "window locut hicut", or "no" to disable. Default is "12 2.2 2.5""", equate=False), #Extension options: _Option(["-gap_trigger", "gap_trigger"], "Number of bits to trigger gapping Default = 22", equate=False), #Miscellaneous options: _Switch(["-use_sw_tback", "use_sw_tback"], "Compute locally optimal Smith-Waterman alignments?"), #PSI-BLAST options _Option(["-num_iterations", "num_iterations"], """Number of iterations to perform. (integer >=1, Default is 1) Incompatible with: remote""", equate=False), _Option(["-out_pssm", "out_pssm"], "File name to store checkpoint file.", filename=True, equate=False), _Option(["-out_ascii_pssm", "out_ascii_pssm"], "File name to store ASCII version of PSSM.", filename=True, equate=False), #PSSM engine options _Option(["-pseudocount", "pseudocount"], "Pseudo-count value used when constructing PSSM (integer, default 0).", equate=False), _Option(["-domain_inclusion_ethresh", "domain_inclusion_ethresh"], """E-value inclusion threshold for alignments with conserved domains. (float, Default is 0.05)""", equate=False), _Option(["-inclusion_ethresh", "inclusion_ethresh"], "E-value inclusion threshold for pairwise alignments (float, Default is 0.002).", equate=False), #DELTA-BLAST options _Option(["-rpsdb", "rpsdb"], "BLAST domain database name (dtring, Default = 'cdd_delta').", equate=False), _Switch(["-show_domain_hits", "show_domain_hits"], """Show domain hits? Incompatible with: remote, subject""") ] _Ncbiblast2SeqCommandline.__init__(self, cmd, **kwargs) def _test(): """Run the Bio.Blast.Applications module's doctests.""" import doctest doctest.testmod(verbose=1) if __name__ == "__main__": #Run the doctests _test()