# fasta36 -E 0.5 -F 0.3 -m 10 prot_s01.fasta protlib.fasta
FASTA searches a protein or DNA sequence data bank
 version 36.3.5c Dec, 2011(preload8)
Please cite:
 W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448

Query: prot_s01.fasta
  1>>>sp|Q9Y2H6|68-133 - 66 aa
Library: protlib.fasta
  1375680 residues in   250 sequences

Statistics: (shuffled [500]) MLE statistics: Lambda= 0.1373;  K=0.00213
 statistics sampled from 219 (219) to 500 sequences
Algorithm: FASTA (3.7 Nov 2010) [optimized]
Parameters: BL50 matrix (15:-5), open/ext: -10/-2
 ktup: 2, E-join: 1 (0.976), E-opt: 0.2 (0.876), width:  16
 Scan time:  0.170

The best scores are:                                      opt bits E(250)
gi|194762369|ref|XP_001963317.1| GF14002 [Drosophi (1761)   85 26.0    0.42
gi|77812697|ref|NP_035782.3| titin isoform N2-A [M (33467)  106 30.2    0.45

>>>sp|Q9Y2H6|68-133, 66 aa vs protlib.fasta library
; pg_name: fasta36
; pg_ver: 36.3.5c Dec, 2011(preload8)
; pg_argv:  fasta36 -E 0.5 -F 0.3 -m 10 prot_s01.fasta protlib.fasta; pg_name_alg: FASTA
; pg_ver_rel: 3.7 Nov 2010
; pg_matrix: BL50 (15:-5)
; pg_open-ext: -10 -2
; pg_ktup: 2
; pg_join_E(): 1 (0.976)
; pg_optcut_E(): 0.2 (0.876)
; mp_extrap: 60000 500
; mp_stats: (shuffled [500]) MLE statistics: Lambda= 0.1373;  K=0.00213
; mp_KS: -0.0000 (N=0) at  20
; mp_Algorithm: FASTA (3.7 Nov 2010) [optimized]
; mp_Parameters: BL50 matrix (15:-5), open/ext: -10/-2  ktup: 2, E-join: 1 (0.976), E-opt: 0.2 (0.876), width:  16
>>gi|194762369|ref|XP_001963317.1| GF14002 [Drosophila ananassae]
; fa_frame: f
; fa_initn:  88
; fa_init1:  68
; fa_opt:  85
; fa_z-score: 95.3
; fa_bits: 26.0
; fa_expect:   0.42
; sw_score: 85
; sw_ident: 0.310
; sw_sim: 0.493
; sw_overlap: 71
>sp|Q9Y2H6|68 ..
; sq_len: 66
; sq_offset: 1
; sq_type: p
; al_start: 6
; al_stop: 66
; al_display_start: 1
-------------------------PNGSVPPIY----VPPGYA---PQV
IEDNGVRRVVVVPQAPEFH---PGSHTVLHRSPHPPLPGFIPVPTMMPPP
P
>gi|194762369|ref|XP_001963317.1| ..
; sq_len: 1761
; sq_offset: 1
; sq_type: p
; al_start: 129
; al_stop: 195
; al_display_start: 99
QMQHIQQQQQQQQQHSPSDGGSSNCSLPASPPLLQQTATPPQGAQIVPPV
CALHHPQQQLALMAAMQHHHPLPPPHA-LHHAPLPPPP---PLPLNPGPP
PHENGHGGVSPNGGGGGSSASSVSSLSSGSGGGGSQGNNNSSNGTNGIGP
PPAAVPLQPGQLYIQYQGEFYPPEYYIAPH
>>gi|77812697|ref|NP_035782.3| titin isoform N2-A [Mus musculus]
; fa_frame: f
; fa_initn: 104
; fa_init1:  92
; fa_opt: 106
; fa_z-score: 94.9
; fa_bits: 30.2
; fa_expect:   0.45
; sw_score: 106
; sw_ident: 0.294
; sw_sim: 0.574
; sw_overlap: 68
>sp|Q9Y2H6|68 ..
; sq_len: 66
; sq_offset: 1
; sq_type: p
; al_start: 1
; al_stop: 66
; al_display_start: 1
------------------------------PNGSVPPIY--VPPGYAPQV
IEDNGVRRVVVVPQAPEFHPGSHTVLHRSPHPPLPGFIPVPTMMPPPP
>gi|77812697|ref|NP_035782.3| ..
; sq_len: 33467
; sq_offset: 1
; sq_type: p
; al_start: 10781
; al_stop: 10848
; al_display_start: 10751
PVPEKKAPPAVVKKPEPPPAKVPEVPKEAPPEKKVPPKKPEAPPAKVPEV
PKEVVTEKKVAVPKKPEVPPAKVPEVPKKPVIEEKPAIPVVEKVASPPAE
VYEEPEEVTAEEEEPAPAVEEEEYEAPPPPAPVPEEPKKVVPEKKFPVIK
KPEAPPPKEPEPEKKVIEKPKLKPRPPARP

>>><<<
>>>///

66 residues in 1 query   sequences
1375680 residues in 250 library sequences
 Tcomplib [36.3.5c Dec, 2011(preload8)] (4 proc in memory [0G])
 start: Tue May  8 01:06:59 2012 done: Tue May  8 01:06:59 2012
 Total Scan time:  0.170 Total Display time:  0.010

Function used was FASTA [36.3.5c Dec, 2011(preload8)]