# Copyright 2009 by Cymon J. Cox.  All rights reserved.
# This code is part of the Biopython distribution and governed by its
# license.  Please see the LICENSE file that should have been included
# as part of this package.
"""Unittests for Bio.Align.Applications interface for PROBCONS."""

import sys
import os
import unittest
from Bio._py3k import StringIO
from Bio import AlignIO, SeqIO, MissingExternalDependencyError
from Bio.Align.Applications import ProbconsCommandline

#Try to avoid problems when the OS is in another language
os.environ['LANG'] = 'C'

probcons_exe = None
if sys.platform=="win32":
    raise MissingExternalDependencyError("PROBCONS not available on Windows")
else:
    from Bio._py3k import getoutput
    output = getoutput("probcons")
    if "not found" not in output and "probcons" in output.lower():
        probcons_exe = "probcons"

if not probcons_exe:
    raise MissingExternalDependencyError(
        "Install PROBCONS if you want to use the Bio.Align.Applications wrapper.")


class ProbconsApplication(unittest.TestCase):

    def setUp(self):
        self.infile1 = "Fasta/fa01"
        self.annotation_outfile = "Fasta/probcons_annot.out"

    def tearDown(self):
        if os.path.isfile(self.annotation_outfile):
            os.remove(self.annotation_outfile)

    def test_Probcons_alignment_fasta(self):
        """Round-trip through app and read fasta alignment from stdout
        """
        cmdline = ProbconsCommandline(probcons_exe, input=self.infile1)
        self.assertEqual(str(cmdline), probcons_exe + " Fasta/fa01")
        self.assertEqual(str(eval(repr(cmdline))), str(cmdline))
        stdout, stderr = cmdline()
        self.assertTrue(stderr.startswith("\nPROBCONS"))
        align = AlignIO.read(StringIO(stdout), "fasta")
        records = list(SeqIO.parse(self.infile1, "fasta"))
        self.assertEqual(len(records), len(align))
        for old, new in zip(records, align):
            self.assertEqual(old.id, new.id)
            self.assertEqual(str(new.seq).replace("-", ""), str(old.seq).replace("-", ""))

    def test_Probcons_alignment_clustalw(self):
        """Round-trip through app and read clustalw alignment from stdout
        """
        cmdline = ProbconsCommandline(probcons_exe)
        cmdline.set_parameter("input", "Fasta/fa01")
        cmdline.clustalw = True
        self.assertEqual(str(cmdline), probcons_exe + " -clustalw Fasta/fa01")
        self.assertEqual(str(eval(repr(cmdline))), str(cmdline))
        stdout, stderr = cmdline()
        self.assertTrue(stderr.strip().startswith("PROBCONS"))
        align = AlignIO.read(StringIO(stdout), "clustal")
        records = list(SeqIO.parse(self.infile1, "fasta"))
        self.assertEqual(len(records), len(align))
        for old, new in zip(records, align):
            self.assertEqual(old.id, new.id)
            self.assertEqual(str(new.seq).replace("-", ""), str(old.seq).replace("-", ""))

    def test_Probcons_complex_commandline(self):
        """Round-trip through app with complex command line and output file
        """
        cmdline = ProbconsCommandline(probcons_exe, pre=1)
        cmdline.set_parameter("input", "Fasta/fa01")
        cmdline.consistency = 4
        cmdline.set_parameter("--iterative-refinement", 222)
        cmdline.set_parameter("a", True)
        cmdline.annot = self.annotation_outfile
        self.assertEqual(str(cmdline), probcons_exe +
                " -c 4 -ir 222 -pre 1 -annot Fasta/probcons_annot.out "
                "-a Fasta/fa01")
        stdout, stderr = cmdline()
        self.assertTrue(stderr.startswith("\nPROBCONS"))
        self.assertTrue(stdout.startswith(">AK1H_ECOLI/1-378"))


if __name__ == "__main__":
    runner = unittest.TextTestRunner(verbosity = 2)
    unittest.main(testRunner=runner)