Sam, I read your X-Landscsape article in Bioinformatics and have tried out the program. It takes awhile to get used to it, but now that I have played with it a bit, I am beginning to see its usefulness. I do have some comments: 1) File formats. It would be nice if the program could read a more common format, such as fasta. Barring that, It would also be helpful if multiple sequences could be read from a single file. That would make it easier to automate launching xland from GDE. 2) Neither the paper nor readme.txt makes it clear how a database is to be made. I have experimented with reading a file containing a bunch of sequences in fasta format, and I guess what xland is doing is to just read in all legal nucleotide characters, including the sequence names, as part of a very long sequence. Probably that doesn't make a significant difference. If my interpretation is correct, then the way to make a database is to create a file containing only the raw sequences, with no names. This will be correct, other than for a few words created by the junctions between sequences. 3) It would be helpful it xland could write out a character landscape to an ASCII file. Such files would be smaller than PostScript, and could be printed on any printer. 4) Unless there's a reason not to do this, it might be best to get rid of the Index button and just have xland automatically index each sequence as it is read in. 5) When I try to print Display's Write menu using 'Send to printer', it appears that the filter commands are executed properly, but I get an error message Sent to printer (Default) xwd-pnm-ps xwd -id 0x6c000dd | /usr/local/pdsoft/bin/xwdtopnm | /usr/local/pdsoft/bin/pnmtops |lpr -P bldgagric.plant_hp3si xwdtopnm: writing PPM file pnmtops: maxval of 1023 is too large for PostScript lpr: stdin: empty input file This may be an artifact that has something to do with pnmtops on our system. 6) It might help to add to your instructions for installation that '/usr/openwin/lib' needs to be in the LD_LIBRARY_PATH environment variable. Normally that should be true, but I had been playing around with LD_LIBRARY_PATH and somehow that got omitted. I guess that's all my comments for now. I'm hoping that landscapes may provide some insight into plant promoters that we are studying that are activated pathogens. Thanks for making the program available. =============================================================================== Brian Fristensky | Department of Plant Science | "... the lingering after-winter, University of Manitoba | the gray season, March and April, Winnipeg, MB R3T 2N2 CANADA | the months God created to show frist@cc.umanitoba.ca | people who don't drink what a Office phone: 204-474-6085 | hangover is like." FAX: 204-474-7528 | - Garrison Keillor http://home.cc.umanitoba.ca/~frist/ WOBEGON BOY ===============================================================================