:outputfile Output text file to deposit data about sequences, alignments, block coordinates, gap sizes and gap positions. Will delete any existing file with the same name. # :proceed Press "proceed" to start the analysis. # :zoomdone zoomdone # :zoom A new window will appear with sequences aligned on the currently selected color. Sequence names and coordinates corresponding to the framed residues will be displayed in the left. # :motif A new window will appear with the list of common triplets of the active color, aligned the way they occur in the selected peak. The number of sequences that would have the listed triplet at the right position in an implicit alignment is also shown. # :parameters To set the maximum span of triplets to "i" and the threshold for regarding a triplet as common to "j" click with the left button over the "i,j" matrix element. The framed number of common triplets will result from this selection. You may confirm this selection with a right click or you may select an other parameter pair with a left mouse click. # :print After a click on this button file "visa.corners" will contain the coordinates of the main canvas. The utility "visadump.com" uses this data to create a Postscipt page from the image on the canvas. Zoom and motif frames are handled similarily, using "zoom.corners" and "motif.corners" auxiliary files and "zoomdump.com" and "motifdump.com" scripts. # :redisplay A repaint event is activated by this button. Use this to make sure your screen reflects the latest selections of peaks, options, alignments, etc. # :align You may select alignment on a single colored peak or on all the colored peaks from this menu. You may also untie an earlier alignment. # :files Define your sequence and data files in this window. # :filesdone Go, and [create] load the specified sequence and data files. # :seqfile Define the file of sequences to be analyzed here. If it is not a GCG dataset format file, use the READSEQ utility and reformat it! # :patfile Define the file of common triplets here. If you have already created it earlier with the GCGPAT utility, check the LOAD box, otherwise click on CREATE, and VISA will create and dump the necesary data file. # :seqload When this box is checked the named file will be loaded. If you intend to replace the pattern file only, uncheck the box. # :patload If you have not created your pattern file yet, check box "create". If you already have a pattern file that you want to load, check box "load". # :namesframe A new frame is activated that displays sequence ID-s, sequence sizes and definition lines. # :namesdone Remove this annotation window # :options You may set several options from the frame that is activated by a click over this button. # :optionsdone Implements all the changes that have been made in this options frame and removes the frame from the screen. # :optionstoggle Control flags for color assignments. OVERPAINT enables consecutive selections to redefine the color of a triplet. If not checked the original color assignments remain in effect. EXPAND PAINT will add new triplets to an existing colored triplet list. If not checked, a new list is created. When checked SUPPRESS makes the repaint process suppress the display of non-colored triplets. # :optionshorizontalscale This option allows you to overwrite the automatic scaling feature of VISA. # :optionsverticalscale This option gives control over the vertical scaling of the histograms. # :optionspalette You may choose between several preset palettes and optimize the image on your specific display and color printer hardware. # :optionsthreshold A single color alignment locates the best match for every sequence to the selected peak. The score for this best match has to exceed the threshold (percent of maximum score) specified here in order be accepted as a meaningful match. Spurious best matches in case of missing peak will be rejected. # :optionsflip Background and foreground colors can be flipped by this option, light and dark colored peaks may both dominate the screen in this way. # :colors In order to select the active color click on one of the colored boxes. This active color is used to color a selected peak, and also by MOTIF and ZOOM buttons. # :decolor Erases earlier color assignments. # :ruler A soft ruler is activated by this button. You may drag this ruler into your main canvas to estimate sequence lengths. # :quit This is the safe exit from VISA. #