import ij.IJ; import ij.ImagePlus; import ij.gui.PolygonRoi; import ij.gui.ProfilePlot; image = IJ.getImage(); roi = image.getRoi(); if (roi == null) { IJ.error("Need a ROI!"); return; } if (roi.isLine()) { new ProfilePlot(image, true).createWindow(); return; } polygon = roi.getPolygon(); // close the polygon polygon.addPoint(polygon.xpoints[0], polygon.ypoints[0]); roi = new PolygonRoi(polygon, roi.POLYLINE); roi.fitSpline(); dummyImage = new ImagePlus(image.getTitle(), image.getProcessor()); dummyImage.setRoi(roi); new ProfilePlot(dummyImage, true).createWindow();