# Albert Cardona 2009-11-16 for Nitai Steinberg # # Select an AreaList in a TrakEM2 project and then run this script. # from ij import IJ, ImageStack, ImagePlus from ij.gui import ShapeRoi from ij.process import ByteProcessor, ShortProcessor from ini.trakem2.display import Display, AreaList, Patch from java.awt import Color def extract_stack_under_arealist(): # Check that a Display is open display = Display.getFront() if display is None: IJ.log("Open a TrakEM2 Display first!") return # Check that an AreaList is selected and active: ali = display.getActive() if ali is None or not isinstance(ali, AreaList): IJ.log("Please select an AreaList first!") return # Get the range of layers to which ali paints: ls = display.getLayerSet() ifirst = ls.indexOf(ali.getFirstLayer()) ilast = ls.indexOf(ali.getLastLayer()) layers = display.getLayerSet().getLayers().subList(ifirst, ilast +1) # Create a stack with the dimensions of ali bounds = ali.getBoundingBox() stack = ImageStack(bounds.width, bounds.height) # Using 16-bit. To change to 8-bit, use GRAY8 and ByteProcessor in the two lines below: type = ImagePlus.GRAY16 ref_ip = ShortProcessor(bounds.width, bounds.height) for layer in layers: area = ali.getArea(layer) z = layer.getZ() ip = ref_ip.createProcessor(bounds.width, bounds.height) if area is None: stack.addSlice(str(z), bp) continue # Create a ROI from the area of ali at layer: aff = ali.getAffineTransformCopy() aff.translate(-bounds.x, -bounds.y) roi = ShapeRoi(area.createTransformedArea(aff)) # Create a cropped snapshot of the images at layer under ali: flat = Patch.makeFlatImage(type, layer, bounds, 1.0, layer.getDisplayables(Patch), Color.black) b = roi.getBounds() flat.setRoi(roi) ip.insert(flat.crop(), b.x, b.y) # Clear the outside of ROI (ShapeRoi is a non-rectangular ROI type) bimp = ImagePlus("", ip) bimp.setRoi(roi) ip.setValue(0) ip.setBackgroundValue(0) IJ.run(bimp, "Clear Outside", "") # Accumulate slices stack.addSlice(str(z), ip) imp = ImagePlus("AreaList stack", stack) imp.setCalibration(ls.getCalibrationCopy()) imp.show() # Execute: extract_stack_under_arealist()