Converting ImaGene Files

1. Click ImaGene tab.
2. Select the Output on the Tool Bar to define what type of file formats you want to produce.
3. Check the Channel A in the input window for selecting channel A data files.
4. Click Select input file ... from the File menu or the icon to open a file chooser window.
5. Select one or more ImaGene files you want to convert. You can press and hold Ctrl key to select multiple files.
6. Click OK button to close the file choose window.
7. Check Channel B for selecting channel B data files and repeat steps 3-6. Alternatively, you can load all files (both channels) in one channel window and move them with following steps.
8. Click one of the file in Channel A window to check if the highlighted files are the pair of the files representing the two channels. If not correct, select the file and click the arrow buttons to move the file within or between the two channel windows. Checking one of the channel boxes indicates that you are manipulating the channel files.
9. If you want the genes (spots) in the output files have the same order as your ImaGene files, uncheck the Sort by Slide Rows and Slide Columns.
10. Check suitable parameters for the Preferred Intensity ... .
11. Click Start converting from the File menu or the icon to process the conversion.

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