#undef _HAVE_CURSES #if _CURSES_LIB == 0 #elif _CURSES_LIB == 1 #include #ifndef NCURSES_VERSION #warning "_CURSES_LIB=1 but NCURSES_VERSION not defined; tview is NOT compiled" #else #define _HAVE_CURSES #endif #elif _CURSES_LIB == 2 #include #define _HAVE_CURSES #else #warning "_CURSES_LIB is not 0, 1 or 2; tview is NOT compiled" #endif #ifdef _HAVE_CURSES #include #include #include #include "bam.h" #include "faidx.h" #include "bam_maqcns.h" char bam_aux_getCEi(bam1_t *b, int i); char bam_aux_getCSi(bam1_t *b, int i); char bam_aux_getCQi(bam1_t *b, int i); #define TV_MIN_ALNROW 2 #define TV_MAX_GOTO 40 #define TV_LOW_MAPQ 10 #define TV_COLOR_MAPQ 0 #define TV_COLOR_BASEQ 1 #define TV_COLOR_NUCL 2 #define TV_COLOR_COL 3 #define TV_COLOR_COLQ 4 #define TV_BASE_NUCL 0 #define TV_BASE_COLOR_SPACE 1 typedef struct { int mrow, mcol; WINDOW *wgoto, *whelp; bam_index_t *idx; bam_lplbuf_t *lplbuf; bam_header_t *header; bamFile fp; int curr_tid, left_pos; faidx_t *fai; bam_maqcns_t *bmc; int ccol, last_pos, row_shift, base_for, color_for, is_dot, l_ref, ins, no_skip, show_name; char *ref; } tview_t; int tv_pl_func(uint32_t tid, uint32_t pos, int n, const bam_pileup1_t *pl, void *data) { tview_t *tv = (tview_t*)data; int i, j, c, rb, attr, max_ins = 0; uint32_t call = 0; if (pos < tv->left_pos || tv->ccol > tv->mcol) return 0; // out of screen // print referece rb = (tv->ref && pos - tv->left_pos < tv->l_ref)? tv->ref[pos - tv->left_pos] : 'N'; for (i = tv->last_pos + 1; i < pos; ++i) { if (i%10 == 0 && tv->mcol - tv->ccol >= 10) mvprintw(0, tv->ccol, "%-d", i+1); c = tv->ref? tv->ref[i - tv->left_pos] : 'N'; mvaddch(1, tv->ccol++, c); } if (pos%10 == 0 && tv->mcol - tv->ccol >= 10) mvprintw(0, tv->ccol, "%-d", pos+1); // print consensus call = bam_maqcns_call(n, pl, tv->bmc); attr = A_UNDERLINE; c = ",ACMGRSVTWYHKDBN"[call>>28&0xf]; i = (call>>8&0xff)/10+1; if (i > 4) i = 4; attr |= COLOR_PAIR(i); if (c == toupper(rb)) c = '.'; attron(attr); mvaddch(2, tv->ccol, c); attroff(attr); if(tv->ins) { // calculate maximum insert for (i = 0; i < n; ++i) { const bam_pileup1_t *p = pl + i; if (p->indel > 0 && max_ins < p->indel) max_ins = p->indel; } } // core loop for (j = 0; j <= max_ins; ++j) { for (i = 0; i < n; ++i) { const bam_pileup1_t *p = pl + i; int row = TV_MIN_ALNROW + p->level - tv->row_shift; if (j == 0) { if (!p->is_del) { if (tv->base_for == TV_BASE_COLOR_SPACE && (c = bam_aux_getCSi(p->b, p->qpos))) { c = bam_aux_getCSi(p->b, p->qpos); // assume that if we found one color, we will be able to get the color error if (tv->is_dot && '-' == bam_aux_getCEi(p->b, p->qpos)) c = bam1_strand(p->b)? ',' : '.'; } else { if (tv->show_name) { char *name = bam1_qname(p->b); c = (p->qpos + 1 >= p->b->core.l_qname)? ' ' : name[p->qpos]; } else { c = bam_nt16_rev_table[bam1_seqi(bam1_seq(p->b), p->qpos)]; if (tv->is_dot && toupper(c) == toupper(rb)) c = bam1_strand(p->b)? ',' : '.'; } } } else c = '*'; } else { // padding if (j > p->indel) c = '*'; else { // insertion if (tv->base_for == TV_BASE_NUCL) { if (tv->show_name) { char *name = bam1_qname(p->b); c = (p->qpos + j + 1 >= p->b->core.l_qname)? ' ' : name[p->qpos + j]; } else { c = bam_nt16_rev_table[bam1_seqi(bam1_seq(p->b), p->qpos + j)]; if (j == 0 && tv->is_dot && toupper(c) == toupper(rb)) c = bam1_strand(p->b)? ',' : '.'; } } else { c = bam_aux_getCSi(p->b, p->qpos + j); if (tv->is_dot && '-' == bam_aux_getCEi(p->b, p->qpos + j)) c = bam1_strand(p->b)? ',' : '.'; } } } if (row > TV_MIN_ALNROW && row < tv->mrow) { int x; attr = 0; if (((p->b->core.flag&BAM_FPAIRED) && !(p->b->core.flag&BAM_FPROPER_PAIR)) || (p->b->core.flag & BAM_FSECONDARY)) attr |= A_UNDERLINE; if (tv->color_for == TV_COLOR_BASEQ) { x = bam1_qual(p->b)[p->qpos]/10 + 1; if (x > 4) x = 4; attr |= COLOR_PAIR(x); } else if (tv->color_for == TV_COLOR_MAPQ) { x = p->b->core.qual/10 + 1; if (x > 4) x = 4; attr |= COLOR_PAIR(x); } else if (tv->color_for == TV_COLOR_NUCL) { x = bam_nt16_nt4_table[bam1_seqi(bam1_seq(p->b), p->qpos)] + 5; attr |= COLOR_PAIR(x); } else if(tv->color_for == TV_COLOR_COL) { x = 0; switch(bam_aux_getCSi(p->b, p->qpos)) { case '0': x = 0; break; case '1': x = 1; break; case '2': x = 2; break; case '3': x = 3; break; case '4': x = 4; break; default: x = bam_nt16_nt4_table[bam1_seqi(bam1_seq(p->b), p->qpos)]; break; } x+=5; attr |= COLOR_PAIR(x); } else if(tv->color_for == TV_COLOR_COLQ) { x = bam_aux_getCQi(p->b, p->qpos); if(0 == x) x = bam1_qual(p->b)[p->qpos]; x = x/10 + 1; if (x > 4) x = 4; attr |= COLOR_PAIR(x); } attron(attr); mvaddch(row, tv->ccol, bam1_strand(p->b)? tolower(c) : toupper(c)); attroff(attr); } } c = j? '*' : rb; if (c == '*') { attr = COLOR_PAIR(8); attron(attr); mvaddch(1, tv->ccol++, c); attroff(attr); } else mvaddch(1, tv->ccol++, c); } tv->last_pos = pos; return 0; } tview_t *tv_init(const char *fn, const char *fn_fa) { tview_t *tv = (tview_t*)calloc(1, sizeof(tview_t)); tv->is_dot = 1; tv->idx = bam_index_load(fn); if (tv->idx == 0) exit(1); tv->fp = bam_open(fn, "r"); bgzf_set_cache_size(tv->fp, 8 * 1024 *1024); assert(tv->fp); tv->header = bam_header_read(tv->fp); tv->lplbuf = bam_lplbuf_init(tv_pl_func, tv); if (fn_fa) tv->fai = fai_load(fn_fa); tv->bmc = bam_maqcns_init(); tv->ins = 1; bam_maqcns_prepare(tv->bmc); initscr(); keypad(stdscr, TRUE); clear(); noecho(); cbreak(); tv->mrow = 24; tv->mcol = 80; getmaxyx(stdscr, tv->mrow, tv->mcol); tv->wgoto = newwin(3, TV_MAX_GOTO + 10, 10, 5); tv->whelp = newwin(29, 40, 5, 5); tv->color_for = TV_COLOR_MAPQ; start_color(); init_pair(1, COLOR_BLUE, COLOR_BLACK); init_pair(2, COLOR_GREEN, COLOR_BLACK); init_pair(3, COLOR_YELLOW, COLOR_BLACK); init_pair(4, COLOR_WHITE, COLOR_BLACK); init_pair(5, COLOR_GREEN, COLOR_BLACK); init_pair(6, COLOR_CYAN, COLOR_BLACK); init_pair(7, COLOR_YELLOW, COLOR_BLACK); init_pair(8, COLOR_RED, COLOR_BLACK); init_pair(9, COLOR_BLUE, COLOR_BLACK); return tv; } void tv_destroy(tview_t *tv) { delwin(tv->wgoto); delwin(tv->whelp); endwin(); bam_lplbuf_destroy(tv->lplbuf); bam_maqcns_destroy(tv->bmc); bam_index_destroy(tv->idx); if (tv->fai) fai_destroy(tv->fai); free(tv->ref); bam_header_destroy(tv->header); bam_close(tv->fp); free(tv); } int tv_fetch_func(const bam1_t *b, void *data) { tview_t *tv = (tview_t*)data; if (tv->no_skip) { uint32_t *cigar = bam1_cigar(b); // this is cheating... int i; for (i = 0; i core.n_cigar; ++i) { if ((cigar[i]&0xf) == BAM_CREF_SKIP) cigar[i] = cigar[i]>>4<<4 | BAM_CDEL; } } bam_lplbuf_push(b, tv->lplbuf); return 0; } int tv_draw_aln(tview_t *tv, int tid, int pos) { // reset clear(); tv->curr_tid = tid; tv->left_pos = pos; tv->last_pos = tv->left_pos - 1; tv->ccol = 0; // print ref and consensus if (tv->fai) { char *str; if (tv->ref) free(tv->ref); str = (char*)calloc(strlen(tv->header->target_name[tv->curr_tid]) + 30, 1); sprintf(str, "%s:%d-%d", tv->header->target_name[tv->curr_tid], tv->left_pos + 1, tv->left_pos + tv->mcol); tv->ref = fai_fetch(tv->fai, str, &tv->l_ref); free(str); } // draw aln bam_lplbuf_reset(tv->lplbuf); bam_fetch(tv->fp, tv->idx, tv->curr_tid, tv->left_pos, tv->left_pos + tv->mcol, tv, tv_fetch_func); bam_lplbuf_push(0, tv->lplbuf); while (tv->ccol < tv->mcol) { int pos = tv->last_pos + 1; if (pos%10 == 0 && tv->mcol - tv->ccol >= 10) mvprintw(0, tv->ccol, "%-d", pos+1); mvaddch(1, tv->ccol++, (tv->ref && pos < tv->l_ref)? tv->ref[pos - tv->left_pos] : 'N'); ++tv->last_pos; } return 0; } static void tv_win_goto(tview_t *tv, int *tid, int *pos) { char str[256], *p; int i, l = 0; wborder(tv->wgoto, '|', '|', '-', '-', '+', '+', '+', '+'); mvwprintw(tv->wgoto, 1, 2, "Goto: "); for (;;) { int c = wgetch(tv->wgoto); wrefresh(tv->wgoto); if (c == KEY_BACKSPACE || c == '\010' || c == '\177') { --l; } else if (c == KEY_ENTER || c == '\012' || c == '\015') { int _tid = -1, _beg, _end; if (str[0] == '=') { _beg = strtol(str+1, &p, 10); if (_beg > 0) { *pos = _beg; return; } } else { bam_parse_region(tv->header, str, &_tid, &_beg, &_end); if (_tid >= 0) { *tid = _tid; *pos = _beg; return; } } } else if (isgraph(c)) { if (l < TV_MAX_GOTO) str[l++] = c; } else if (c == '\027') l = 0; else if (c == '\033') return; str[l] = '\0'; for (i = 0; i < TV_MAX_GOTO; ++i) mvwaddch(tv->wgoto, 1, 8 + i, ' '); mvwprintw(tv->wgoto, 1, 8, "%s", str); } } static void tv_win_help(tview_t *tv) { int r = 1; WINDOW *win = tv->whelp; wborder(win, '|', '|', '-', '-', '+', '+', '+', '+'); mvwprintw(win, r++, 2, " -=- Help -=- "); r++; mvwprintw(win, r++, 2, "? This window"); mvwprintw(win, r++, 2, "Arrows Small scroll movement"); mvwprintw(win, r++, 2, "h,j,k,l Small scroll movement"); mvwprintw(win, r++, 2, "H,J,K,L Large scroll movement"); mvwprintw(win, r++, 2, "ctrl-H Scroll 1k left"); mvwprintw(win, r++, 2, "ctrl-L Scroll 1k right"); mvwprintw(win, r++, 2, "space Scroll one screen"); mvwprintw(win, r++, 2, "backspace Scroll back one screen"); mvwprintw(win, r++, 2, "g Go to specific location"); mvwprintw(win, r++, 2, "m Color for mapping qual"); mvwprintw(win, r++, 2, "n Color for nucleotide"); mvwprintw(win, r++, 2, "b Color for base quality"); mvwprintw(win, r++, 2, "c Color for cs color"); mvwprintw(win, r++, 2, "z Color for cs qual"); mvwprintw(win, r++, 2, ". Toggle on/off dot view"); mvwprintw(win, r++, 2, "s Toggle on/off ref skip"); mvwprintw(win, r++, 2, "r Toggle on/off rd name"); mvwprintw(win, r++, 2, "N Turn on nt view"); mvwprintw(win, r++, 2, "C Turn on cs view"); mvwprintw(win, r++, 2, "i Toggle on/off ins"); mvwprintw(win, r++, 2, "q Exit"); r++; mvwprintw(win, r++, 2, "Underline: Secondary or orphan"); mvwprintw(win, r++, 2, "Blue: 0-9 Green: 10-19"); mvwprintw(win, r++, 2, "Yellow: 20-29 White: >=30"); wrefresh(win); wgetch(win); } void tv_loop(tview_t *tv) { int tid, pos; tid = tv->curr_tid; pos = tv->left_pos; while (1) { int c = getch(); switch (c) { case '?': tv_win_help(tv); break; case '\033': case 'q': goto end_loop; case '/': case 'g': tv_win_goto(tv, &tid, &pos); break; case 'm': tv->color_for = TV_COLOR_MAPQ; break; case 'b': tv->color_for = TV_COLOR_BASEQ; break; case 'n': tv->color_for = TV_COLOR_NUCL; break; case 'c': tv->color_for = TV_COLOR_COL; break; case 'z': tv->color_for = TV_COLOR_COLQ; break; case 's': tv->no_skip = !tv->no_skip; break; case 'r': tv->show_name = !tv->show_name; break; case KEY_LEFT: case 'h': --pos; break; case KEY_RIGHT: case 'l': ++pos; break; case KEY_SLEFT: case 'H': pos -= 20; break; case KEY_SRIGHT: case 'L': pos += 20; break; case '.': tv->is_dot = !tv->is_dot; break; case 'N': tv->base_for = TV_BASE_NUCL; break; case 'C': tv->base_for = TV_BASE_COLOR_SPACE; break; case 'i': tv->ins = !tv->ins; break; case '\010': pos -= 1000; break; case '\014': pos += 1000; break; case ' ': pos += tv->mcol; break; case KEY_UP: case 'j': --tv->row_shift; break; case KEY_DOWN: case 'k': ++tv->row_shift; break; case KEY_BACKSPACE: case '\177': pos -= tv->mcol; break; case KEY_RESIZE: getmaxyx(stdscr, tv->mrow, tv->mcol); break; default: continue; } if (pos < 0) pos = 0; if (tv->row_shift < 0) tv->row_shift = 0; tv_draw_aln(tv, tid, pos); } end_loop: return; } int bam_tview_main(int argc, char *argv[]) { tview_t *tv; if (argc == 1) { fprintf(stderr, "Usage: bamtk tview [ref.fasta]\n"); return 1; } tv = tv_init(argv[1], (argc == 2)? 0 : argv[2]); tv_draw_aln(tv, 0, 0); tv_loop(tv); tv_destroy(tv); return 0; } #else // #ifdef _HAVE_CURSES #include #warning "No curses library is available; tview is disabled." int bam_tview_main(int argc, char *argv[]) { fprintf(stderr, "[bam_tview_main] The ncurses library is unavailable; tview is not compiled.\n"); return 1; } #endif // #ifdef _HAVE_CURSES