/***************************************************************************** # Copyright (C) 1994-2008 by David Gordon. # All rights reserved. # # This software is part of a beta-test version of the Consed/Autofinish # package. It should not be redistributed or # used for any commercial purpose, including commercially funded # sequencing, without written permission from the author and the # University of Washington. # # This software is provided ``AS IS'' and any express or implied # warranties, including, but not limited to, the implied warranties of # merchantability and fitness for a particular purpose, are disclaimed. # In no event shall the authors or the University of Washington be # liable for any direct, indirect, incidental, special, exemplary, or # consequential damages (including, but not limited to, procurement of # substitute goods or services; loss of use, data, or profits; or # business interruption) however caused and on any theory of liability, # whether in contract, strict liability, or tort (including negligence # or otherwise) arising in any way out of the use of this software, even # if advised of the possibility of such damage. # # Building Consed from source is error prone and not simple which is # why I provide executables. Due to time limitations I cannot # provide any assistance in building Consed. Even if you do not # modify the source, you may introduce errors due to using a # different version of the compiler, a different version of motif, # different versions of other libraries than I used, etc. For this # reason, if you discover Consed bugs, I can only offer help with # those bugs if you first reproduce those bugs with an executable # provided by me--not an executable you have built. # # Modifying Consed is also difficult. Although Consed is modular, # some modules are used by many other modules. Thus making a change # in one place can have unforeseen effects on many other features. # It may takes months for you to notice these other side-effects # which may not seen connected at all. It is not feasable for me to # provide help with modifying Consed sources because of the # potentially huge amount of time involved. # #*****************************************************************************/ #ifndef autoFinishExpTag_include #define autoFinishExpTag_include #include "autoFinishPurposes.h" #include "tag.h" #include "rwtvalorderedvector.h" #include "readTypes.h" class autoFinishExpTag : public tag { public: autoFinishExpTag( Contig* pContig, const int nConsPosStart, const int nConsPosEnd, const RWCString& soSource, const RWCString& soTagDate, const RWCString& soTagData ) : // If these stay -1, that will indicate that the ace // file did not contain values for them and they are not known. nSingleSubcloneBasesFixed_( -1 ), dErrorsFixed_( -1 ), nExtendingIntoGapThisManyBases_( -1 ), tag( NULL, pContig, "autoFinishExp", nConsPosStart, nConsPosEnd, false, // bWriteToPhdFileNotAceFile "", // soComment soSource, soTagDate, false ) // bNoTrans { soMiscData_ = soTagData; } virtual RWCString soGetExtraInformationForNavigator(const int nMaxLength ); bool bExperimentWorked(); void getUnpaddedHighQualityRegion( int& nUnpaddedHighQualityRegionLeft, int& nUnpaddedHighQualityRegionRight, int& nUnpaddedHighQualityRegionLeftOnConsensus, int& nUnpaddedHighQualityRegionRightOnConsensus, bool& bCompletelyOffConsensus ); void howWellDidExpClusterExtendContig(); void howWellDidExpClusterFixErrors(); void howWellDidExpClusterFlankGap(); void evaluateExperimentCluster(); void howWellDidExpClusterCoverSingleSubcloneBases(); virtual void flipOrientation() { bComplementedFromWayPhrapCreatedContig_ = !bComplementedFromWayPhrapCreatedContig_; } public: RWCString soPrimerBases_; int nMeltingTemp_; RWTValOrderedVector aTemplates_; RWTValOrderedVector aExpID_; bool bComplementedFromWayPhrapCreatedContig_; // in the case of flank gap experiments, this indicates the strand // of the *new* read. It is precisely the opposite strand from the // existing universal primer read. int nUnpaddedOffsetToStartOfHighQualityRegion_; int nUnpaddedOffsetToEndOfHighQualityRegion_; int nUnpaddedOffsetOfBeginningOfReadFromTag_; double dErrorsFixed_; int nSingleSubcloneBasesFixed_; int nReadType_; int nPurpose_; RWCString soPrimerID_; int nExtendingIntoGapThisManyBases_; // int nExtendsIntoWhichGap_; --not useful since phrap may have // flipped the contig. transferConsensusTags.perl would then have // to change this field. // before these suggested experiments, how many // problems were present. This will help in determining // how well these experiments worked. int nOriginalNumberOfSingleSubcloneBases_; double dOriginalNumberOfErrors_; }; #endif