/***************************************************************************** # Copyright (C) 1994-2008 by David Gordon. # All rights reserved. # # This software is part of a beta-test version of the Consed/Autofinish # package. It should not be redistributed or # used for any commercial purpose, including commercially funded # sequencing, without written permission from the author and the # University of Washington. # # This software is provided ``AS IS'' and any express or implied # warranties, including, but not limited to, the implied warranties of # merchantability and fitness for a particular purpose, are disclaimed. # In no event shall the authors or the University of Washington be # liable for any direct, indirect, incidental, special, exemplary, or # consequential damages (including, but not limited to, procurement of # substitute goods or services; loss of use, data, or profits; or # business interruption) however caused and on any theory of liability, # whether in contract, strict liability, or tort (including negligence # or otherwise) arising in any way out of the use of this software, even # if advised of the possibility of such damage. # # Building Consed from source is error prone and not simple which is # why I provide executables. Due to time limitations I cannot # provide any assistance in building Consed. Even if you do not # modify the source, you may introduce errors due to using a # different version of the compiler, a different version of motif, # different versions of other libraries than I used, etc. For this # reason, if you discover Consed bugs, I can only offer help with # those bugs if you first reproduce those bugs with an executable # provided by me--not an executable you have built. # # Modifying Consed is also difficult. Although Consed is modular, # some modules are used by many other modules. Thus making a change # in one place can have unforeseen effects on many other features. # It may takes months for you to notice these other side-effects # which may not seen connected at all. It is not feasable for me to # provide help with modifying Consed sources because of the # potentially huge amount of time involved. # #*****************************************************************************/ #include "clScaffold.h" #include "contig.h" #include "editaction.h" #include "consed.h" #include "edit_complement_contig.h" void clScaffold :: setScaffoldLength() { Contig* pLastContig = aContigs_[ aContigs_.length() - 1 ]; nScaffoldLength_ = pLastContig->nClonePosRight_; } RWCString clScaffold :: soGetDescription() { RWCString soScaffoldMap; soScaffoldMap.increaseMaxLength( (size_t) ( aContigs_.length() * 5 ) ); for( int nContig = 0; nContig < aContigs_.length(); ++nContig ) { Contig* pContig = aContigs_[ nContig ]; if ( nContig == 0 ) { if ( pContig->bThisContigIsComplementedInTheScaffold_ ) { if ( pContig->bIsThisEndTheEndOfTheClone( nRightGap ) ) { soScaffoldMap += "E-"; } } else { if ( pContig->bIsThisEndTheEndOfTheClone( nLeftGap ) ) { soScaffoldMap += "E-"; } } } else { soScaffoldMap += "-"; } soScaffoldMap += pContig->soGetAbbreviatedName(); if ( pContig->bThisContigIsComplementedInTheScaffold_ ) soScaffoldMap += "c"; if ( nContig == ( aContigs_.length() - 1 ) ) { if ( pContig->bThisContigIsComplementedInTheScaffold_ ) { if ( pContig->bIsThisEndTheEndOfTheClone( nLeftGap ) ) { soScaffoldMap += "-E"; } } else { if ( pContig->bIsThisEndTheEndOfTheClone( nRightGap ) ) { soScaffoldMap += "-E"; } } } } return( soScaffoldMap ); } void clScaffold :: removeTheCs() { for( int nContig = 0; nContig < aContigs_.length(); ++nContig ) { Contig* pContig = aContigs_[ nContig ]; if ( pContig->bThisContigIsComplementedInTheScaffold_ ) { if ( nContig == 0 ) { if ( bUseFirstContigUnpaddedStart_ ) { // must changed nFirstContigUnpaddedStart_ into new coordinates nFirstContigUnpaddedStart_ = pContig->nComplementUnpaddedIndex( nFirstContigUnpaddedStart_ ); } } if ( nContig == aContigs_.length() - 1 ) { if ( bUseLastContigUnpaddedEnd_ ) { nLastContigUnpaddedEnd_ = pContig->nComplementUnpaddedIndex( nLastContigUnpaddedEnd_ ); } } cerr << "complementing " << pContig->soGetName() << endl; // complement the contig EditAction *pEditAction = new EditComplementContig( pContig ); ConsEd::pGetConsEd()->doEditAction( pEditAction, true ); // write to .wrk } else { cerr << "not complementing " << pContig->soGetName() << endl; } } // ConsEd::pGetConsEd()->refreshAllContigWinsAndAllTeditors(); } void clScaffold :: fixOrientation( RWCString& soErrorMessage ) { // each contig should have a WR item added to one read indicating // its position. for( int nContig = 0; nContig < aContigs_.length(); ++nContig ) { Contig* pContig = aContigs_[ nContig ]; RWCString soErrorMessage2; pContig->fixOrientation( soErrorMessage2 ); soErrorMessage += soErrorMessage2; } } void clScaffold :: flipScaffold() { // -1 means the scaffold is user-defined, as in the digest if ( nScaffold_ != -1 ) ConsEd::pGetAssembly()->reverseScaffold( nScaffold_ ); aContigs_.reverseElementOrder(); aIfContigsAreComplemented_.reverseElementOrder(); for( int nContig = 0; nContig < aIfContigsAreComplemented_.length(); ++nContig ) { aIfContigsAreComplemented_[ nContig ] = !aIfContigsAreComplemented_[ nContig ]; } bool bTemp; bTemp = bFirstContigIsCloneEnd_; bFirstContigIsCloneEnd_ = bLastContigIsCloneEnd_; bLastContigIsCloneEnd_ = bTemp; // don't know if this is necessary aContigEndPairs_.reverseElementOrder(); bTemp = bUseFirstContigUnpaddedStart_; bUseFirstContigUnpaddedStart_ = bUseLastContigUnpaddedEnd_; bUseLastContigUnpaddedEnd_ = bTemp; int nTemp = nFirstContigUnpaddedStart_; nFirstContigUnpaddedStart_ = nLastContigUnpaddedEnd_; nLastContigUnpaddedEnd_ = nTemp; // I do not believe these positions need to be complemented. // For example, suppose nFirstContigUnpaddedStart_ = 1 in ContigA. // After flipping the scaffold, ContigA will be the last contig, // and will be ContigAc (complemented in the scaffold) so position 1 will // be the last position in the scaffold, which is what we want. // We do not want to change this to 32123 (or whatever the last position // in that contig is). } void clScaffold :: unfixOrientation() { for( int nContig = 0; nContig < aContigs_.length(); ++nContig ) { Contig* pContig = aContigs_[ nContig ]; pContig->unfixOrientation(); } }