/***************************************************************************** # Copyright (C) 1994-2008 by David Gordon. # All rights reserved. # # This software is part of a beta-test version of the Consed/Autofinish # package. It should not be redistributed or # used for any commercial purpose, including commercially funded # sequencing, without written permission from the author and the # University of Washington. # # This software is provided ``AS IS'' and any express or implied # warranties, including, but not limited to, the implied warranties of # merchantability and fitness for a particular purpose, are disclaimed. # In no event shall the authors or the University of Washington be # liable for any direct, indirect, incidental, special, exemplary, or # consequential damages (including, but not limited to, procurement of # substitute goods or services; loss of use, data, or profits; or # business interruption) however caused and on any theory of liability, # whether in contract, strict liability, or tort (including negligence # or otherwise) arising in any way out of the use of this software, even # if advised of the possibility of such damage. # # Building Consed from source is error prone and not simple which is # why I provide executables. Due to time limitations I cannot # provide any assistance in building Consed. Even if you do not # modify the source, you may introduce errors due to using a # different version of the compiler, a different version of motif, # different versions of other libraries than I used, etc. For this # reason, if you discover Consed bugs, I can only offer help with # those bugs if you first reproduce those bugs with an executable # provided by me--not an executable you have built. # # Modifying Consed is also difficult. Although Consed is modular, # some modules are used by many other modules. Thus making a change # in one place can have unforeseen effects on many other features. # It may takes months for you to notice these other side-effects # which may not seen connected at all. It is not feasable for me to # provide help with modifying Consed sources because of the # potentially huge amount of time involved. # #*****************************************************************************/ #include "copyPrimerMatchesFromShorterToLongerPrimers.h" #include "primerType.h" #include "sortPrimersByEndPosition.h" #include "sortPrimersByStartPosition.h" #include "consedParameters.h" #include "assert.h" // copySinglePrimerField takes // copySinglePrimerField( nScoreOfStickiestFalseMatch_ ) // and makes // pPrimer->nScoreOfStickiestFalseMatch_ = shortPrimer.nScoreOfStickiestFalseMatch; #define copySinglePrimerField( fieldName ) \ pPrimer->fieldName = shortPrimer.fieldName void copyPrimerMatchesFromShorterToLongerPrimers( primerType* pPrimerArray, const int nNumberOfPrimers, const bool bForwardNotReversePrimer ) { // sort primers by their 3' end so primers with the same 3' end // can have false and vector match information copied from // shorter to longer // reverse primers actually end at pPrimer->nUnpaddedStart_ so // to sort them by their 3' end (in preparation for copying the // false match and vector matches), sort them by the start position if ( bForwardNotReversePrimer ) sortPrimersByEndPosition( pPrimerArray, nNumberOfPrimers ); else sortPrimersByStartPosition( pPrimerArray, nNumberOfPrimers ); primerType shortPrimer; shortPrimer.nUnpaddedEnd_ = -1000; // something absurd for( int nPrimer = 0; nPrimer < nNumberOfPrimers; ++nPrimer ) { primerType* pPrimer = pPrimerArray + nPrimer; if ( pPrimer->nUnpaddedLength_ == consedParameters::pGetConsedParameters()->nPrimersMinimumLengthOfAPrimerToUse_ ) { shortPrimer = *pPrimer; } else { if (bForwardNotReversePrimer ) { assert( pPrimer->nUnpaddedEnd_ == shortPrimer.nUnpaddedEnd_ ); } else { assert( pPrimer->nUnpaddedStart_ == shortPrimer.nUnpaddedStart_ ); } // fixed bug. Used to copy bAcceptable_ no matter what. // But the problem is that a longer primer could be // unacceptable due to overlapping a high quality discrepancy // while the shorter primer is acceptable. Do not make // an unacceptable primer be acceptable! if ( pPrimer->bAcceptable_ ) { copySinglePrimerField( bAcceptable_ ); copySinglePrimerField( nWhyIsPrimerNotAcceptable_ ); } copySinglePrimerField( nScoreOfStickiestFalseMatch_ ); copySinglePrimerField( pContigOfStickiestFalseMatch_ ); copySinglePrimerField( nUnpaddedOfStickiestFalseMatch_ ); copySinglePrimerField( bContigOfStickiestFalseMatchIsComplemented_ ); copySinglePrimerField( nSequenceInFileStickiestScore_ ); copySinglePrimerField( nSequenceInFileSequenceNumber_ ); copySinglePrimerField( nSequenceInFileBasePosition_ ); copySinglePrimerField( bSequenceInFileIsComplemented_ ); } } }