/***************************************************************************** # Copyright (C) 1994-2008 by David Gordon. # All rights reserved. # # This software is part of a beta-test version of the Consed/Autofinish # package. It should not be redistributed or # used for any commercial purpose, including commercially funded # sequencing, without written permission from the author and the # University of Washington. # # This software is provided ``AS IS'' and any express or implied # warranties, including, but not limited to, the implied warranties of # merchantability and fitness for a particular purpose, are disclaimed. # In no event shall the authors or the University of Washington be # liable for any direct, indirect, incidental, special, exemplary, or # consequential damages (including, but not limited to, procurement of # substitute goods or services; loss of use, data, or profits; or # business interruption) however caused and on any theory of liability, # whether in contract, strict liability, or tort (including negligence # or otherwise) arising in any way out of the use of this software, even # if advised of the possibility of such damage. # # Building Consed from source is error prone and not simple which is # why I provide executables. Due to time limitations I cannot # provide any assistance in building Consed. Even if you do not # modify the source, you may introduce errors due to using a # different version of the compiler, a different version of motif, # different versions of other libraries than I used, etc. For this # reason, if you discover Consed bugs, I can only offer help with # those bugs if you first reproduce those bugs with an executable # provided by me--not an executable you have built. # # Modifying Consed is also difficult. Although Consed is modular, # some modules are used by many other modules. Thus making a change # in one place can have unforeseen effects on many other features. # It may takes months for you to notice these other side-effects # which may not seen connected at all. It is not feasable for me to # provide help with modifying Consed sources because of the # potentially huge amount of time involved. # #*****************************************************************************/ #ifndef digestForOneEnzyme_included #define digestForOneEnzyme_included #include "rwtptrorderedvector.h" #include "arrayOfRestrictionFragments.h" class restrictionFragment; class guiDisplayDigest; class digestForOneEnzyme { public: // these are for building the aPredictedResFragsBySize_ arrayOfRestrictionFragments aPredictedRestrictionFragmentsNotEndingAtVectorInsertJunction_; arrayOfRestrictionFragments aPredictedInsertRestrictionFragmentsEndingAtVectorInsertJunction_; arrayOfRestrictionFragments aPredictedVectorRestrictionFragmentsEndingAtVectorInsertJunction_; bool bNoCutSitesInVector_; arrayOfRestrictionFragments aActualRestrictionFragments_; arrayOfRestrictionFragments aPredictedResFragsBySize_; // this is the same array as above, but sorted by position in scaffold arrayOfRestrictionFragments aPredictedResFragsByConsPos_; RWCString soEnzymeName_; RWCString soEnzymeBases_; int nMaxGelPosition_; guiDisplayDigest* pGuiDisplayDigest_; int nAssemblyViewEnzymeLine_; public: digestForOneEnzyme() : bNoCutSitesInVector_( false ), nAssemblyViewEnzymeLine_( -1 ) { aPredictedRestrictionFragmentsNotEndingAtVectorInsertJunction_.soName_ = "digestForOneEnzyme::aPredictedRestrictionFragmentsNotEndingAtVectorInsertJunction_"; aPredictedInsertRestrictionFragmentsEndingAtVectorInsertJunction_.soName_ = "digestForOneEnzyme::aPredictedInsertRestrictionFragmentsEndingAtVectorInsertJunction_"; aPredictedVectorRestrictionFragmentsEndingAtVectorInsertJunction_.soName_ = "digestForOneEnzyme::aPredictedVectorRestrictionFragmentsEndingAtVectorInsertJunction_"; aActualRestrictionFragments_.soName_ = "digestForOneEnzyme::aActualRestrictionFragments_"; aPredictedResFragsBySize_.soName_ = "digestForOneEnzyme::aPredictedResFragsBySize_"; aPredictedResFragsByConsPos_.soName_ = "digestForOneEnzyme::aPredictedResFragsByConsPos_"; } ~digestForOneEnzyme(); bool operator==( const digestForOneEnzyme& dig2 ) const { return( this == &dig2 ); } void processingAfterFindingAllPartialFragments( const bool bFlipVectorFromTheDefault); void combineFragments( restrictionFragment* pInsertFrag, const int nWhichEndOfInsert, const int nWhichEndOfVector, restrictionFragment*& pNewFrag ); void combineFragments2( restrictionFragment* pPartialInsertFragment, restrictionFragment* pPartialVectorFragment, restrictionFragment*& pNewFrag ); void combineRemainingVectorAndInsertPartialFragments( const bool bFlipVectorFromDefault ); restrictionFragment* pGetClosestAvailableFragment( restrictionFragment* pFrag, RWTPtrOrderedVector* pFragmentArray ); restrictionFragment* pGetClosestAvailableFragment2( restrictionFragment* pFrag, RWTPtrOrderedVector* pFragmentArray ); void relatePredictedAndActualFragments(); bool bRelateMorePredictedAndActualFragments(); void dealWithPartialFragments( const bool bFlipVectorFromDefault ); }; #endif