/***************************************************************************** # Copyright (C) 1994-2008 by David Gordon. # All rights reserved. # # This software is part of a beta-test version of the Consed/Autofinish # package. It should not be redistributed or # used for any commercial purpose, including commercially funded # sequencing, without written permission from the author and the # University of Washington. # # This software is provided ``AS IS'' and any express or implied # warranties, including, but not limited to, the implied warranties of # merchantability and fitness for a particular purpose, are disclaimed. # In no event shall the authors or the University of Washington be # liable for any direct, indirect, incidental, special, exemplary, or # consequential damages (including, but not limited to, procurement of # substitute goods or services; loss of use, data, or profits; or # business interruption) however caused and on any theory of liability, # whether in contract, strict liability, or tort (including negligence # or otherwise) arising in any way out of the use of this software, even # if advised of the possibility of such damage. # # Building Consed from source is error prone and not simple which is # why I provide executables. Due to time limitations I cannot # provide any assistance in building Consed. Even if you do not # modify the source, you may introduce errors due to using a # different version of the compiler, a different version of motif, # different versions of other libraries than I used, etc. For this # reason, if you discover Consed bugs, I can only offer help with # those bugs if you first reproduce those bugs with an executable # provided by me--not an executable you have built. # # Modifying Consed is also difficult. Although Consed is modular, # some modules are used by many other modules. Thus making a change # in one place can have unforeseen effects on many other features. # It may takes months for you to notice these other side-effects # which may not seen connected at all. It is not feasable for me to # provide help with modifying Consed sources because of the # potentially huge amount of time involved. # #*****************************************************************************/ #include "editMakeHighQualityAndChangeConsensus.h" #include "consedParameters.h" void editMakeHighQualityAndChangeConsensus :: doEditSpecific() { assert ( pLocatedFragment_); Contig* pContig = pLocatedFragment_->pGetContig(); assert( nMinConsensusPosition_ <= nMaxConsensusPosition_ ); if ( nMinConsensusPosition_ < pContig->nGetStartConsensusIndex() ) { ostringstream ost; ost << "You cannot extend the consensus on the left end, but you can extend it on the right end. So complement, extend, and complement back." << ends; InputDataError ide(ost.str().c_str()); throw ide; } bExtendingConsensus_ = false; if ( nMaxConsensusPosition_ > pContig->nGetEndConsensusIndex() ) { if ( nMinConsensusPosition_ == ( pContig->nGetEndConsensusIndex() + 1 )) { bExtendingConsensus_ = true; nOldLengthOfConsensus_ = pContig->nGetPaddedSeqLength(); } else { ostringstream ost; ost << "If you want to extend the consensus, you must swipe starting from the base precisly after the end of the consensus." << ends; InputDataError ide(ost.str().c_str()); throw ide; } } // save old read Ntides int nCons; for ( nCons = nMinConsensusPosition_; nCons <= nMaxConsensusPosition_; nCons++) { daReadNtidesBeforeEdit_.append( pLocatedFragment_->ntGetFragFromConsPos( nCons ) ); } bool bThereArePads = false; if (!bExtendingConsensus_ ) { // save the consensus Ntides for ( nCons = nMinConsensusPosition_; nCons <= nMaxConsensusPosition_; nCons++) { // save the old value of the consensus ntide at that pos daConsNtidesBeforeEdit_.append(pContig->ntGetCons(nCons)); if (pContig->ntGetCons(nCons).bIsPad() ) bThereArePads = true; } } if ( bExtendingConsensus_ ) { if ( pCP->bExtendConsensusWithHighQuality_ ) { // make the read high quality pLocatedFragment_->makeHighQuality( nMinConsensusPosition_, nMaxConsensusPosition_ ); } for( nCons = nMinConsensusPosition_; nCons <= nMaxConsensusPosition_; ++nCons ) { pContig->appendNtide( pLocatedFragment_->ntGetFragFromConsPos(nCons) ); // this really isn't necessary since we are going to // setUnpaddedPositionsArray() whenever we are extending the // consensus regardless whether there are pads or not if (pContig->ntGetCons( nCons ).bIsPad() ) bThereArePads = true; } // extend the aligned region of the read, per request from // Eugene Goltsman, 2005 if ( pLocatedFragment_->bIsWholeReadUnaligned() ) { pLocatedFragment_->nAlignClipStart_ = nMinConsensusPosition_; pLocatedFragment_->nAlignClipEnd_ = nMaxConsensusPosition_; } else { // this is a little dangerous--we are going to extend // the aligned region to be the union of the old, the new, // and anything in between int nNewAlignClipStart = MIN( pLocatedFragment_->nAlignClipStart_, nMinConsensusPosition_ ); int nNewAlignClipEnd = MAX( pLocatedFragment_->nAlignClipEnd_, nMaxConsensusPosition_ ); pLocatedFragment_->nAlignClipStart_ = nNewAlignClipStart; pLocatedFragment_->nAlignClipEnd_ = nNewAlignClipEnd; } } else { // make the read high quality pLocatedFragment_->makeHighQuality( nMinConsensusPosition_, nMaxConsensusPosition_ ); // copy the read to the consensus for ( nCons = nMinConsensusPosition_; nCons <= nMaxConsensusPosition_; nCons++) { pContig->setNtideAtPos(nCons, pLocatedFragment_->ntGetFragFromConsPos(nCons) ); if (pContig->ntGetCons( nCons ).bIsPad() ) bThereArePads = true; } } if (bThereArePads || bExtendingConsensus_ ) pContig->setUnpaddedPositionsArray(); // change the base segment array pBaseSegChanges_ = pContig->baseSegArray_.setBaseSegToFragRegion(pLocatedFragment_, nMinConsensusPosition_, nMaxConsensusPosition_ ); } void editMakeHighQualityAndChangeConsensus :: undoEditSpecific() { // restore base segment array changes Contig* pContig = pLocatedFragment_->pGetContig(); pContig->baseSegArray_.undoBaseSegChanges(pBaseSegChanges_); if ( bExtendingConsensus_ ) { int nBasesToDelete = pContig->nGetPaddedConsLength() - nOldLengthOfConsensus_; assert( nBasesToDelete > 0 ); for( int nBases = 1; nBases <= nBasesToDelete; ++nBases ) { pContig->removeLastBase(); } } // restore the consensus sequence for (int n = 0; n < daConsNtidesBeforeEdit_.length(); n++) { // replace the old value of the consensus ntide at that pos pContig->setNtideAtPos(n+nMinConsensusPosition_,daConsNtidesBeforeEdit_[n]); } pContig->setUnpaddedPositionsArray(); // restore old read Ntides for (int nCons = nMinConsensusPosition_; nCons <= nMaxConsensusPosition_; nCons++) { pLocatedFragment_->overstrikeNtide( nCons, daReadNtidesBeforeEdit_[ nCons - nMinConsensusPosition_ ], false ); } }