/***************************************************************************** # Copyright (C) 1994-2008 by David Gordon. # All rights reserved. # # This software is part of a beta-test version of the Consed/Autofinish # package. It should not be redistributed or # used for any commercial purpose, including commercially funded # sequencing, without written permission from the author and the # University of Washington. # # This software is provided ``AS IS'' and any express or implied # warranties, including, but not limited to, the implied warranties of # merchantability and fitness for a particular purpose, are disclaimed. # In no event shall the authors or the University of Washington be # liable for any direct, indirect, incidental, special, exemplary, or # consequential damages (including, but not limited to, procurement of # substitute goods or services; loss of use, data, or profits; or # business interruption) however caused and on any theory of liability, # whether in contract, strict liability, or tort (including negligence # or otherwise) arising in any way out of the use of this software, even # if advised of the possibility of such damage. # # Building Consed from source is error prone and not simple which is # why I provide executables. Due to time limitations I cannot # provide any assistance in building Consed. Even if you do not # modify the source, you may introduce errors due to using a # different version of the compiler, a different version of motif, # different versions of other libraries than I used, etc. For this # reason, if you discover Consed bugs, I can only offer help with # those bugs if you first reproduce those bugs with an executable # provided by me--not an executable you have built. # # Modifying Consed is also difficult. Although Consed is modular, # some modules are used by many other modules. Thus making a change # in one place can have unforeseen effects on many other features. # It may takes months for you to notice these other side-effects # which may not seen connected at all. It is not feasable for me to # provide help with modifying Consed sources because of the # potentially huge amount of time involved. # #*****************************************************************************/ #include "editOverstrikeAllReadsAtConsPos.h" #include "locatedFragment.h" #include "contig.h" #include "consed.h" void editOverstrikeAllReadsAtConsPos :: doEditSpecific() { cNewConsensusBase_ = pMasterLocFrag_->ntGetFragFromConsPos( nConsPos_ ).cGetBase(); bool bNewNtideIsPad = ( cNewConsensusBase_ == '*' ? true : false ); for( int nRead = 0; nRead < aArrayOfReadsToOverstrike_.length(); ++nRead ) { LocatedFragment* pLocFrag = aArrayOfReadsToOverstrike_[ nRead ]; char cOldBase = pLocFrag->ntGetFragFromConsPos( nConsPos_ ).cGetBase(); Quality qualOld = pLocFrag->ntGetFragFromConsPos( nConsPos_ ).qualGetQuality(); aOldBases_.insert( cOldBase ); aOldQualities_.insert( qualOld ); aOldTagArrays_.insert( pLocFrag->aTags_ ); if ( cOldBase == cNewConsensusBase_ ) { aWasReadOverstruck_.insert( false ); continue; } else { aWasReadOverstruck_.insert( true ); } // if reached here, the read must be changed pLocFrag->makeCopyOfPhredCallsIfNecessary(); int nFragIndex = pLocFrag->nGetFragIndexFromConsPos( nConsPos_ ); pLocFrag->Sequence::setBaseAtPos( nFragIndex, cNewConsensusBase_ ); pLocFrag->setQualityAtConsPos( nConsPos_, ucQualityLowEdited ); bool bOldNtideWasPad = ( cOldBase == '*' ? true : false ); pLocFrag->addEditTag( bOldNtideWasPad, bNewNtideIsPad, nConsPos_ ); } // and now fix the consensus Contig* pContig = pMasterLocFrag_->pContig_; cOldConsensusBase_ = pContig->ntGetCons( nConsPos_ ).cGetBase(); qualOldConsensusQuality_ = pContig->ntGetCons( nConsPos_ ).qualGetQuality(); pContig->Sequence::setBaseAtPos( nConsPos_, cNewConsensusBase_ ); pContig->Sequence::setQualityAtSeqPos( nConsPos_, ucQualityLowEdited ); // added May 2009 (DG) when noticed that when click on a base in // Aligned Reads Window, wrong unpadded position is displayed bool bOldNtideIsPad = ( cOldConsensusBase_ == '*' ? true : false ); if ( bOldNtideIsPad != bNewNtideIsPad ) { pContig->setUnpaddedPositionsArray(); } // end of added May 2009 } void editOverstrikeAllReadsAtConsPos :: undoEditSpecific() { for( int nRead = 0; nRead < aArrayOfReadsToOverstrike_.length(); ++nRead ) { LocatedFragment* pLocFrag = aArrayOfReadsToOverstrike_[ nRead ]; char cOldBase = aOldBases_[ nRead ]; Quality qualOld = aOldQualities_[ nRead ]; if ( cOldBase != pLocFrag->ntGetFragFromConsPos( nConsPos_ ).cGetBase() ) { int nFragPos = pLocFrag->nGetFragIndexFromConsPos( nConsPos_ ); pLocFrag->Sequence::setBaseAtPos( nFragPos, cOldBase ); pLocFrag->Sequence::setQualityAtSeqPos( nFragPos, qualOld ); // how shall I remove the edit tag? pLocFrag->aTags_ = aOldTagArrays_[ nRead ]; // and how about the phredBaseCalls? There is no reason // to remove them. They do not change. } } // and now reset the consensus Contig* pContig = pMasterLocFrag_->pContig_; pContig->Sequence::setBaseAtPos( nConsPos_, cOldConsensusBase_ ); pContig->Sequence::setQualityAtSeqPos( nConsPos_, qualOldConsensusQuality_ ); // added May 2009 (DG) when noticed that when click on a base in // Aligned Reads Window, wrong unpadded position is displayed bool bOldNtideIsPad = ( cOldConsensusBase_ == '*' ? true : false ); bool bNewNtideIsPad = ( cNewConsensusBase_ == '*' ? true : false ); if ( bOldNtideIsPad != bNewNtideIsPad ) { pContig->setUnpaddedPositionsArray(); } // end of added May 2009 } void editOverstrikeAllReadsAtConsPos :: writeEditToEditHistoryFile() { Contig* pContig = pMasterLocFrag_->pGetContig(); RWCString soLineToWrite( (size_t) 1000 ); soLineToWrite.nCurrentLength_ = sprintf( soLineToWrite.data(), "changeAllReads %s %s %d", pContig->soGetName().data(), pMasterLocFrag_->soGetName().data(), nConsPos_ ); assert( soLineToWrite.nCurrentLength_ > 0 ); for( int nRead = 0; nRead < aArrayOfReadsToOverstrike_.length(); ++nRead ) { LocatedFragment* pLocFrag = aArrayOfReadsToOverstrike_[ nRead ]; soLineToWrite += " "; soLineToWrite += aArrayOfReadsToOverstrike_[ nRead ]->soGetName(); } ConsEd::pGetAssembly()->writeEditToEditHistoryFile( soLineToWrite.data() ); } RWCString editOverstrikeAllReadsAtConsPos :: soGetDescriptionOfEdit() { Contig* pContig = pMasterLocFrag_->pGetContig(); char cBase = pMasterLocFrag_->ntGetFragFromConsPos( nConsPos_ ).cGetBase(); RWCString soDescription = "changeAllReads in " + pContig->soGetName() + " to match " + pMasterLocFrag_->soGetName() + " at " + RWCString( (long) pContig->nUnpaddedIndex( nConsPos_ ) ) + " which is " + RWCString( cBase ); return( soDescription ); } void editOverstrikeAllReadsAtConsPos :: setChangedIfNecessary() { Contig* pContig = pMasterLocFrag_->pContig_; bOldContigChanged_ = pContig->bChanged(); bOldAssemblyChanged_ = ConsEd::pGetAssembly()->bChanged(); for( int nRead = 0; nRead < aArrayOfReadsToOverstrike_.length(); ++nRead ) { LocatedFragment* pLocFrag = aArrayOfReadsToOverstrike_[ nRead ]; aOldReadsChanged_.insert( pLocFrag->bChanged() ); if ( aWasReadOverstruck_[ nRead ] ) { pLocFrag->setChanged(); } } } void editOverstrikeAllReadsAtConsPos :: undoSetChangedIfNecessary() { for( int nRead = 0; nRead < aArrayOfReadsToOverstrike_.length(); ++nRead ) { if ( !aWasReadOverstruck_[ nRead ] ) continue; LocatedFragment* pLocFrag = aArrayOfReadsToOverstrike_[ nRead ]; if ( !aOldReadsChanged_[ nRead ] ) { pLocFrag->setUnchanged(); } } if ( ! bOldContigChanged_ ) { pMasterLocFrag_->pGetContig()->setUnchanged(); } if ( ! bOldAssemblyChanged_ ) { ConsEd::pGetAssembly()->setUnchanged(); } }