/***************************************************************************** # Copyright (C) 1994-2008 by David Gordon. # All rights reserved. # # This software is part of a beta-test version of the Consed/Autofinish # package. It should not be redistributed or # used for any commercial purpose, including commercially funded # sequencing, without written permission from the author and the # University of Washington. # # This software is provided ``AS IS'' and any express or implied # warranties, including, but not limited to, the implied warranties of # merchantability and fitness for a particular purpose, are disclaimed. # In no event shall the authors or the University of Washington be # liable for any direct, indirect, incidental, special, exemplary, or # consequential damages (including, but not limited to, procurement of # substitute goods or services; loss of use, data, or profits; or # business interruption) however caused and on any theory of liability, # whether in contract, strict liability, or tort (including negligence # or otherwise) arising in any way out of the use of this software, even # if advised of the possibility of such damage. # # Building Consed from source is error prone and not simple which is # why I provide executables. Due to time limitations I cannot # provide any assistance in building Consed. Even if you do not # modify the source, you may introduce errors due to using a # different version of the compiler, a different version of motif, # different versions of other libraries than I used, etc. For this # reason, if you discover Consed bugs, I can only offer help with # those bugs if you first reproduce those bugs with an executable # provided by me--not an executable you have built. # # Modifying Consed is also difficult. Although Consed is modular, # some modules are used by many other modules. Thus making a change # in one place can have unforeseen effects on many other features. # It may takes months for you to notice these other side-effects # which may not seen connected at all. It is not feasable for me to # provide help with modifying Consed sources because of the # potentially huge amount of time involved. # #*****************************************************************************/ #ifndef fixContigEnd_included #define fixContigEnd_included #include "rwtptrorderedvector.h" #include "filename.h" #include "compareContigsTypes.h" #include "contigSegment.h" #include "rwtvalorderedvector.h" #include "assert.h" class Contig; class LocatedFragment; class Assembly; class paddedPieceOfRead; class fixContigEnd { public: Contig* pBigContig_; int nWhichEnd_; Contig* pMiniContig_; Assembly* pMiniAssembly_; int nLeftmostConsPosOfProtrudingReads_; RWTPtrOrderedVector aProtrudingReads_; RWTPtrOrderedVector aNewReads_; paddedPieceOfRead* pPaddedPieceOfReadBigC_; paddedPieceOfRead* pPaddedPieceOfReadMini_; // for cleanup RWCString soBaseNameForTemporaryFiles_; // for joining contigs Contig* pNewContig_; RWTValOrderedVector contigSegments_; int nInNewJoinedContigAlignedRegionLeftConsPos_; int nInNewJoinedContigAlignedRegionRightConsPos_; public: fixContigEnd( Contig* pBigContig, const int nWhichEnd ) : pBigContig_( pBigContig ), nWhichEnd_( nWhichEnd ), pMiniAssembly_( NULL ) {} void doIt( Contig*& pContig ); void createNewContig(); void alignMiniContigToBigContig( bool& bError ); void reportMessage( const bool bError, const RWCString& soError ); void openAceFileAndChooseContig( const FileName& filAceFileName, bool& bError ); int nOpenAceFileAndGetNumberOfContigs( const FileName& filAceFileName ); void moveMiniAssemblyIntoMainAssembly(); void addColumnsOfPadsToAlignContigs(); bool bCheckAddColumnsOfPadsToAlignContigs(); bool bContigSegmentsOK( ); void calculateTotalQualitiesOfContigSegment( contigSegment& cs ); void getBestContigSegment( const int nContigSeg, Contig*& pBestContig, int& nBestPaddedStart, int& nBestPaddedEnd ); void addContigSegment( const int nUnpaddedStart1, const int nUnpaddedEnd1, const int nUnpaddedStart2, const int nUnpaddedEnd2 ); void findEndOfDiscrepantSegment( const int nPaddedStart, // zero-based paddedPieceOfRead coordinates int& nPaddedEnd, // zero-based paddedPieceOfRead coordinates int& nUnpaddedEnd1, // in unpadded pContig1_ coordinates int& nUnpaddedEnd2, // in unpadded pContig2_ coordinates bool& bEndOfAlignment ); void findEndOfMatchSegment( const int nStartPadded, int& nEndPadded, int& nEndUnpadded1, int& nEndUnpadded2, bool& bMatchSegmentExists ); paddedPieceOfRead* pGetPaddedPieceOfRead( const compareContigsTypes eContig1OrContig2 ) { if (eContig1OrContig2 == eContig1 ) return( pPaddedPieceOfReadBigC_ ); else if ( eContig1OrContig2 == eContig2 ) return( pPaddedPieceOfReadMini_ ); else assert( false ); } void makeContigSegmentsContiguous( Contig* pLeftContig, const int nNewConsPosFromNotLeftConsPos ); void dumpContigSegments(); void cleanUp(); void getLeftAndRightEndsOfFakeRead( int& nPaddedLeftEndOfFakeRead, int& nPaddedRightEndOfFakeRead ); }; #endif