/***************************************************************************** # Copyright (C) 1994-2008 by David Gordon. # All rights reserved. # # This software is part of a beta-test version of the Consed/Autofinish # package. It should not be redistributed or # used for any commercial purpose, including commercially funded # sequencing, without written permission from the author and the # University of Washington. # # This software is provided ``AS IS'' and any express or implied # warranties, including, but not limited to, the implied warranties of # merchantability and fitness for a particular purpose, are disclaimed. # In no event shall the authors or the University of Washington be # liable for any direct, indirect, incidental, special, exemplary, or # consequential damages (including, but not limited to, procurement of # substitute goods or services; loss of use, data, or profits; or # business interruption) however caused and on any theory of liability, # whether in contract, strict liability, or tort (including negligence # or otherwise) arising in any way out of the use of this software, even # if advised of the possibility of such damage. # # Building Consed from source is error prone and not simple which is # why I provide executables. Due to time limitations I cannot # provide any assistance in building Consed. Even if you do not # modify the source, you may introduce errors due to using a # different version of the compiler, a different version of motif, # different versions of other libraries than I used, etc. For this # reason, if you discover Consed bugs, I can only offer help with # those bugs if you first reproduce those bugs with an executable # provided by me--not an executable you have built. # # Modifying Consed is also difficult. Although Consed is modular, # some modules are used by many other modules. Thus making a change # in one place can have unforeseen effects on many other features. # It may takes months for you to notice these other side-effects # which may not seen connected at all. It is not feasable for me to # provide help with modifying Consed sources because of the # potentially huge amount of time involved. # #*****************************************************************************/ #include "nFindScoreOfSelfMatch.h" #include "nNumberFromBase.h" #include "nPrimerFalseMatchScoreArray.h" #define nPenaltyForNotStartingAt3PrimeEnd -2 int nFindScoreOfSelfMatch( const int nZeroBasedStartPosition, const int nZeroBasedEndPosition, const int nUncovered3PrimeBases, char* szComplementedPrimer, const int nCompPrimerStart, int& nPosOfMaxCumulativeScore ) { // szComplementedPrimer address is set so that, however it is positioned, // // Create sub-oligos that start at the most 3' end of the primer // (hence start at nZeroBasedEndPosition) and find the one that has // the highest score. int nCumulativeScore = nUncovered3PrimeBases * nPenaltyForNotStartingAt3PrimeEnd; // the purpose of this is to penalize matches that don't start at // the 3' end of the primer. The further the match from the 3' end, // the more the penalty int nMaxCumulativeScore = -10000; // something absurd for( int nSubOligo5PrimeEnd = nZeroBasedEndPosition; nSubOligo5PrimeEnd >= nZeroBasedStartPosition; --nSubOligo5PrimeEnd ) { char cBaseInComplementedPrimer = szComplementedPrimer[ nSubOligo5PrimeEnd - nCompPrimerStart ]; nCumulativeScore += nPrimerFalseMatchScoreArray[ nSubOligo5PrimeEnd ][ nNumberFromBase[ cBaseInComplementedPrimer ] ]; if (nCumulativeScore > nMaxCumulativeScore ) { nMaxCumulativeScore = nCumulativeScore; nPosOfMaxCumulativeScore = nSubOligo5PrimeEnd; } } return( nMaxCumulativeScore ); }