/***************************************************************************** # Copyright (C) 1994-2008 by David Gordon. # All rights reserved. # # This software is part of a beta-test version of the Consed/Autofinish # package. It should not be redistributed or # used for any commercial purpose, including commercially funded # sequencing, without written permission from the author and the # University of Washington. # # This software is provided ``AS IS'' and any express or implied # warranties, including, but not limited to, the implied warranties of # merchantability and fitness for a particular purpose, are disclaimed. # In no event shall the authors or the University of Washington be # liable for any direct, indirect, incidental, special, exemplary, or # consequential damages (including, but not limited to, procurement of # substitute goods or services; loss of use, data, or profits; or # business interruption) however caused and on any theory of liability, # whether in contract, strict liability, or tort (including negligence # or otherwise) arising in any way out of the use of this software, even # if advised of the possibility of such damage. # # Building Consed from source is error prone and not simple which is # why I provide executables. Due to time limitations I cannot # provide any assistance in building Consed. Even if you do not # modify the source, you may introduce errors due to using a # different version of the compiler, a different version of motif, # different versions of other libraries than I used, etc. For this # reason, if you discover Consed bugs, I can only offer help with # those bugs if you first reproduce those bugs with an executable # provided by me--not an executable you have built. # # Modifying Consed is also difficult. Although Consed is modular, # some modules are used by many other modules. Thus making a change # in one place can have unforeseen effects on many other features. # It may takes months for you to notice these other side-effects # which may not seen connected at all. It is not feasable for me to # provide help with modifying Consed sources because of the # potentially huge amount of time involved. # #*****************************************************************************/ #include "consedParameters.h" #include "whichPrimersHaveAcceptableMeltingTemp.h" #include "primerType.h" #include "dGetPrimerMeltingTemperature.h" #include "whyIsPrimerNotAcceptableTypes.h" void whichPrimersHaveAcceptableMeltingTemp( primerType* pPrimerArray, const int nNumberOfPrimers, bool& bSomeAcceptablePrimers ) { bSomeAcceptablePrimers = false; double dMinTemp = (double) consedParameters::pGetConsedParameters()->nPrimersMinMeltingTempToUse_; double dMaxTemp = (double) consedParameters::pGetConsedParameters()->nPrimersMaxMeltingTempToUse_; for( int nPrimer = 0; nPrimer < nNumberOfPrimers; ++nPrimer ) { primerType* pPrimer = pPrimerArray + nPrimer; if (! pPrimer->bAcceptable_ ) continue; pPrimer->dMeltingTemperature_ = dGetPrimerMeltingTemperature( pPrimer->szPrimer_, pPrimer->nUnpaddedLength_ ); if ( ( dMinTemp <= pPrimer->dMeltingTemperature_ ) && ( pPrimer->dMeltingTemperature_ <= dMaxTemp ) ) bSomeAcceptablePrimers = true; else { pPrimer->bAcceptable_ = false; if ( pPrimer->dMeltingTemperature_ < dMinTemp ) pPrimer->nWhyIsPrimerNotAcceptable_ = BAD_PRIMER_MELTING_TEMPERATURE_TOO_LOW; else pPrimer->nWhyIsPrimerNotAcceptable_ = BAD_PRIMER_MELTING_TEMPERATURE_TOO_HIGH; } } }