ENTRY           A34313  #type complete
TITLE           antifreeze protein II precursor - sea raven
ALTERNATE_NAMES pro-antifreeze protein type II
ORGANISM        #formal_name Hemitripterus americanus #common_name sea raven
DATE            10-Sep-1999 #sequence_revision 10-Sep-1999 #text_change
                10-Sep-1999
ACCESSIONS      A34313; A24602; PC2386; PC2387; S65733
REFERENCE       A34313
   #authors     Hayes, P.H.; Scott, G.K.; Ng, N.F.L.; Hew, C.L.; Davies,
                P.L.
   #journal     J. Biol. Chem. (1989) 264:18761-18767
   #title       Cystine-rich type II antifreeze protein precursor is
                initiated from the third AUG codon of its mRNA.
   #cross-references MUID:90036986
   #accession   A34313
      ##molecule_type DNA; mRNA
      ##residues 1-37,'G',39-163 ##label HAY
      ##cross-references GB:J05100; NID:g213875; PIDN:AAA49618.1;
                PID:g213876
      ##note there are 12-15 copies in the genome; the sequence of SR7 was
                determined
      ##note the initiator codon was determined
      ##note parts of this sequence, including the amino end of the mature
                protein, were determined by protein sequencing
      ##note the amino end of the mature protein is blocked
REFERENCE       A24602
   #authors     Ng, N.F.; Trinh, K.Y.; Hew, C.L.
   #journal     J. Biol. Chem. (1986) 261:15690-15695
   #title       Structure of an antifreeze polypeptide precursor from the
                sea raven, Hemitripterus americanus.
   #cross-references MUID:87057207
   #accession   A24602
      ##molecule_type mRNA
      ##residues 'MQRQQADTETREDISTAGLSIIFIVCTISTTR',1-163 ##label NGN
      ##cross-references GB:J02593; NID:g213873; PIDN:AAA49617.1;
                PID:g213874
      ##note parts of this sequence were determined by protein sequencing
REFERENCE       PC2386
   #authors     Duncker, B.P.; Gauthier, S.Y.; Davies, P.L.
   #journal     Biochem. Biophys. Res. Commun. (1994) 203:1851-1857
   #title       Cystine-rich fish antifreeze is produced as an active
                proprotein precursor in fall armyworm cells.
   #cross-references MUID:95032070
   #accession   PC2386
      ##molecule_type mRNA
      ##residues 1-45 ##label DUN
   #accession   PC2387
      ##molecule_type protein
      ##residues 18-27 ##label DU2
REFERENCE       S65733
   #authors     Duncker, B.P.; Gauthier, S.Y.; Davies, P.L.
   #journal     Biochim. Biophys. Acta (1996) 1292:312-316
   #title       Evidence for a proprotein intermediate during maturation of
                type II antifreeze protein in sea raven, Hemitripterus
                americanus.
   #cross-references MUID:96176860
   #accession   S65733
      ##molecule_type protein
      ##residues 18-22 ##label DUW
GENETICS
   #introns     21/1; 66/3; 105/3; 142/1
CLASSIFICATION  #superfamily tetranectin; C-type lectin homology
KEYWORDS        pyroglutamic acid
FEATURE
   1-17                #domain signal sequence #status predicted #label SIG\
   18-34               #domain propeptide #status experimental #label PRO\
   35-163              #product antifreeze protein II #status predicted
                       #label MAT\
   41-159              #domain C-type lectin homology #label LCH\
   35                  #modified_site pyrrolidone carboxylic acid (Gln) (in
                       mature form) #status experimental\
   41-52,69-159,
   135-151             #disulfide_bonds #status predicted
SUMMARY         #length 163  #molecular-weight 17509  #checksum 1944
SEQUENCE
                5        10        15        20        25        30
      1 M L T V S L L V C A M M A L T Q A N D D K I L K G T A T E A
     31 G P V S Q R A P P N C P A G W Q P L G D R C I Y Y E T T A M
     61 T W A L A E T N C M K L G G H L A S I H S Q E E H S F I Q T
     91 L N A G V V W I G G S A C L Q A G A W T W S D G T P M N F R
    121 S W C S T K P D D V L A A C C M Q M T A A A D Q C W D D L P
    151 C P A S H K S V C A M T F
///