{ name "PBI101TD" type "DNA" longname Cloning vector pBI101 sequence-ID "U12639" descrip "GUS gene fusion vector pBI101 T-DNA region." creator "Jefferson,R.A., Burgess,S.M. and Hirsh,D." creation-date 5/16/97 20:56:49 direction 1 strandedness 1 comments "KEYWORDS pBI101; T-DNA; GUS gene fusion vector; neomycin phosphotransferase; beta-glucuronidase. SOURCE Cloning vector pBI101. artificial sequence; vectors. REFERENCE 1 (sites) TITLE beta-Glucuronidase from Escherichia coli as a gene-fusion marker JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83 (22), 8447-8451 (1986) MEDLINE 87041472 REFERENCE 2 (bases 2497 to 2556) AUTHORS Jefferson,R. TITLE Assaying chimeric genes in plants: the GUS gene fusion system JOURNAL Plant Mol. Biol. Rep. 5, 387-405 (1987) REFERENCE 3 (bases 1 to 5349) AUTHORS Jefferson,R.A., Kavanagh,T.A. and Bevan,M.W. TITLE GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants JOURNAL EMBO J. 6 (13), 3901-3907 (1987) MEDLINE 88166629 REFERENCE 4 (bases 1 to 5349) AUTHORS Wei,W. and Lindsey,K. TITLE T-DNA sequence of the GUS gene fusion vector pBI101 JOURNAL Unpublished COMMENT NCBI gi: 2088506 FEATURES Location/Qualifiers source 1..5349 /organism=`Cloning vector pBI101` /plasmid=`pBI101` /note=`sequence from the right border to the left border` misc_feature 1..2497 /note=`pBin19 sequence, containing a neomycin phosphotransferase gene driven by nos promoter` repeat_unit 1..25 /note=`the right border repeat` CDS 385..1179 /note=`NCBI gi: 2102680` /codon_start=1 /transl_table=11 /product=`neomycin phosphotransferase` /translation=`MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF` misc_feature 2497..2537 /note=`polylinker` CDS 2551..4359 /note=`modified beta-glucuronidase gene coding region from pRAJ260; NCBI gi: 529330` /codon_start=1 /transl_table=11 /product=`beta-glucuronidase` /translation=`MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI KELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDA HTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ` 3'UTR 4430..4682 /note=`3'UTR of the nopaline synthase gene` misc_feature 4686..5349 /note=`pBin19 sequence (see also GenBank Accession Number U09365)` repeat_unit 5325..5349 /note=`the left border repeat` BASE COUNT 1273 a 1346 c 1417 g 1313 t " sequence " gtttacccgccaatatatcctgtcaaacactgatagtttaaactgaaggcgggaaacgac aatctgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcggg acaagccgttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgc gggtttctggagtttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaa agtcgcctaaggtcactatcagctagcaaatatttcttgtcaaaaatgctccactgacgt tccataaattcccctcggtatccaattagagtctcatattcactctcaatccaaataatc tgcaccggatctggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccg gccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctct gatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgac ctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacg acgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctg ctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaa gtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgccca ttcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtctt gtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgcc aggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctg ggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagctt ggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcag cgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgaaa tgaccgaccaagcgacgcccaacctgccatcacgagatttcgattccaccgccgccttct atgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcg gggatctcatgctggagttcttcgcccacgggatctctgcggaacaggcggtcgaaggtg ccgatatcattacgacagcaacggccgacaagcacaacgccacgatcctgagcgacaata tgatcgggcccggcgtccacatcaacggcgtcggcggcgactgcccaggcaagaccgaga tgcaccgcgatatcttgctgcgttcggatattttcgtggagttcccgccacagacccgga tgatccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccg gtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaaca tgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattataca tttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcgg tgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtggtggt tctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggca aacgctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgct aaaggcaaacttgattctgtcgctactgattacggtgctgctatcgatggtttcattggt gacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaattcc caaatggctcaagtcggtgacggtgataattcacctttaatgaataatttccgtcaatat ttaccttccctccctcaatcggttgaatgtcgcccttttgtctttggcccaatacgcaaa ccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgac tggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccc caggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaa tttcacacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcaggtcgac tctagaggatccccgggtggtcagtcccttatgttacgtcctgtagaaaccccaacccgt gaaatcaaaaaactcgacggcctgtgggcattcagtctggatcgcgaaaactgtggaatt gatcagcgttggtgggaaagcgcgttacaagaaagccgggcaattgctgtgccaggcagt tttaacgatcagttcgccgatgcagatattcgtaattatgcgggcaacgtctggtatcag cgcgaagtctttataccgaaaggttgggcaggccagcgtatcgtgctgcgtttcgatgcg gtcactcattacggcaaagtgtgggtcaataatcaggaagtgatggagcatcagggcggc tatacgccatttgaagccgatgtcacgccgtatgttattgccgggaaaagtgtacgtatc accgtttgtgtgaacaacgaactgaactggcagactatcccgccgggaatggtgattacc gacgaaaacggcaagaaaaagcagtcttacttccatgatttctttaactatgccggaatc catcgcagcgtaatgctctacaccacgccgaacacctgggtggacgatatcaccgtggtg acgcatgtcgcgcaagactgtaaccacgcgtctgttgactggcaggtggtggccaatggt gatgtcagcgttgaactgcgtgatgcggatcaacaggtggttgcaactggacaaggcact agcgggactttgcaagtggtgaatccgcacctctggcaaccgggtgaaggttatctctat gaactgtgcgtcacagccaaaagccagacagagtgtgatatctacccgcttcgcgtcggc atccggtcagtggcagtgaagggccaacagttcctgattaaccacaaaccgttctacttt actggctttggtcgtcatgaagatgcggacttacgtggcaaaggattcgataacgtgctg atggtgcacgaccacgcattaatggactggattggggccaactcctaccgtacctcgcat tacccttacgctgaagagatgctcgactgggcagatgaacatggcatcgtggtgattgat gaaactgctgctgtcggctttaacctctctttaggcattggtttcgaagcgggcaacaag ccgaaagaactgtacagcgaagaggcagtcaacggggaaactcagcaagcgcacttacag gcgattaaagagctgatagcgcgtgacaaaaaccacccaagcgtggtgatgtggagtatt gccaacgaaccggatacccgtccgcaagtgcacgggaatatttcgccactggcggaagca acgcgtaaactcgacccgacgcgtccgatcacctgcgtcaatgtaatgttctgcgacgct cacaccgataccatcagcgatctctttgatgtgctgtgcctgaaccgttattacggatgg tatgtccaaagcggcgatttggaaacggcagagaaggtactggaaaaagaacttctggcc tggcaggagaaactgcatcagccgattatcatcaccgaatacggcgtggatacgttagcc gggctgcactcaatgtacaccgacatgtggagtgaagagtatcagtgtgcatggctggat atgtatcaccgcgtctttgatcgcgtcagcgccgtcgtcggtgaacaggtatggaatttc gccgattttgcgacctcgcaaggcatattgcgcgttggcggtaacaagaaagggatcttc actcgcgaccgcaaaccgaagtcggcggcttttctgctgcaaaaacgctggactggcatg aacttcggtgaaaaaccgcagcagggaggcaaacaatgaatcaacaactctcctggcgca ccatcgtcggctacagcctcgggaattgctaccgagctcgaatttccccgatcgttcaaa catttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcat ataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatt tatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaa caaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactaga tcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttaccc aacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggccc gcaccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttc ttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctc cctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggt gatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggag tccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcg ggctattcttttgatttataagggattttgccgatttcggaaccaccatcaaacaggatt ttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcgg tgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccccagtacatt aaaaacgtccgcaatgtgttattaagttgtctaagcgtcaatttgtttacaccacaatat atcctgcca" } { name "ARBLKSP" type "DNA" longname synthetic construct sequence-ID "X52331" descrip "pBluescript KS(+) vector DNA, phagemid excised from lambda ZAP." creator "Thomas,E.A." creation-date 6/11/98 21:30: 6 circular 1 direction 1 strandedness 1 comments "NID g58065KEYWORDS artificial sequence; cloning vector; expression vector; vector. SOURCE synthetic construct. artificial sequence. REFERENCE 1 (bases 1 to 2958) TITLE Direct Submission JOURNAL Submitted (20-FEB-1990) Thomas E.A., Stratagene Clonin Systems, 11099 North Torney Pines Rd., La Jolla, CA 92037, USA REFERENCE 2 (bases 1 to 2958) AUTHORS Short,J.M., Fernandez,J.M., Sorge,J.A. and Huse,W.D. TITLE Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties JOURNAL Nucleic Acids Res. 16 (15), 7583-7600 (1988) MEDLINE 88319944 REFERENCE 3 (bases 1 to 2958) AUTHORS Alting-Mees,M.A. and Short,J.M. TITLE pBluescript II: gene mapping vectors JOURNAL Nucleic Acids Res. 17 (22), 9494 (1989) MEDLINE 90067967 REFERENCE 4 (bases 1 to 2958) AUTHORS Lampe,D.J., Grant,T.E. and Robertson,H.M. TITLE Factors affecting transposition of the Himar1 mariner transposon in vitro JOURNAL Genetics 149 (1), 179-187 (1998) MEDLINE 98250682 FEATURES Location/Qualifiers source 1..2958 /organism=`synthetic construct` /db_xref=`taxon:32630` misc_feature 1..2958 /note=`phagemid pBluescript KS(+)` BASE COUNT 749 a 734 c 751 g 724 t " sequence " cacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcag ctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagac cgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtgga ctccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatc accctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagg gagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaa gaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaac caccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggct gcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaa agggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacg ttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattggagctcca ccgcggtggcggccgctctagaactagtggatcccccgggctgcaggaattcgatatcaa gcttatcgataccgtcgacctcgagggggggcccggtacccagcttttgttccctttagt gagggttaatttcgagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgtt atccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtg cctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgg gaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgc gtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgc ggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccg cgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgct caagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaa gctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttc tcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcg ccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactgg cagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttct tgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgc tgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctc aagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgtt aagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaa aatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaat gcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct gactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctg caatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccag ccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctatta attgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttg ccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccg gttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagct ccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggtta tggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactg gtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcc cggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattg gaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcga tgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctg ggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaat gttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtc tcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgca catttccccgaaaagtgc" } { name "pBSGUS.dna" type "DNA" sequence-ID "SunOS:2:948166914" descrip "pBSGUS.dna, 4809 bases, 91D4272 checksum." direction 1 strandedness 1 comments " " sequence " GATCCCCGGGTGGTCAGTCCCTTATGTTACGTCCTGTAGAAACCCCAACCCGTGAAATCA AAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAACTGTGGAATTGATCAGC GTTGGTGGGAAAGCGCGTTACAAGAAAGCCGGGCAATTGCTGTGCCAGGCAGTTTTAACG ATCAGTTCGCCGATGCAGATATTCGTAATTATGCGGGCAACGTCTGGTATCAGCGCGAAG TCTTTATACCGAAAGGTTGGGCAGGCCAGCGTATCGTGCTGCGTTTCGATGCGGTCACTC ATTACGGCAAAGTGTGGGTCAATAATCAGGAAGTGATGGAGCATCAGGGCGGCTATACGC CATTTGAAGCCGATGTCACGCCGTATGTTATTGCCGGGAAAAGTGTACGTATCACCGTTT GTGTGAACAACGAACTGAACTGGCAGACTATCCCGCCGGGAATGGTGATTACCGACGAAA ACGGCAAGAAAAAGCAGTCTTACTTCCATGATTTCTTTAACTATGCCGGAATCCATCGCA GCGTAATGCTCTACACCACGCCGAACACCTGGGTGGACGATATCACCGTGGTGACGCATG TCGCGCAAGACTGTAACCACGCGTCTGTTGACTGGCAGGTGGTGGCCAATGGTGATGTCA GCGTTGAACTGCGTGATGCGGATCAACAGGTGGTTGCAACTGGACAAGGCACTAGCGGGA CTTTGCAAGTGGTGAATCCGCACCTCTGGCAACCGGGTGAAGGTTATCTCTATGAACTGT GCGTCACAGCCAAAAGCCAGACAGAGTGTGATATCTACCCGCTTCGCGTCGGCATCCGGT CAGTGGCAGTGAAGGGCCAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGCT TTGGTCGTCATGAAGATGCGGACTTACGTGGCAAAGGATTCGATAACGTGCTGATGGTGC ACGACCACGCATTAATGGACTGGATTGGGGCCAACTCCTACCGTACCTCGCATTACCCTT ACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGTGGTGATTGATGAAACTG CTGCTGTCGGCTTTAACCTCTCTTTAGGCATTGGTTTCGAAGCGGGCAACAAGCCGAAAG AACTGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGCAAGCGCACTTACAGGCGATTA AAGAGCTGATAGCGCGTGACAAAAACCACCCAAGCGTGGTGATGTGGAGTATTGCCAACG AACCGGATACCCGTCCGCAAGTGCACGGGAATATTTCGCCACTGGCGGAAGCAACGCGTA AACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCGACGCTCACACCG ATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGATGGTATGTCC AAAGCGGCGATTTGGAAACGGCAGAGAAGGTACTGGAAAAAGAACTTCTGGCCTGGCAGG AGAAACTGCATCAGCCGATTATCATCACCGAATACGGCGTGGATACGTTAGCCGGGCTGC ACTCAATGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATC ACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATT TTGCGACCTCGCAAGGCATATTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCG ACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCG GTGAAAAACCGCAGCAGGGAGGCAAACAATGAATCAACAACTCTCCTGGCGCACCATCGT CGGCTACAGCCTCGGGAATTGCTACCGAGCTCCAATTCGCCCTATAGTGAGTCGTATTAC AATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTT AATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACC GATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGC GCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCC CTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCC CGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTC GACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACG GTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACT GGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATT TCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAA ATATTAACGCTTACAATTTAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTT GTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAA TGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTA TTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAG TAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACA GCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTA AAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTC GCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATC TTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACA CTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGC ACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCA TACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAAC TATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGG CGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTG ATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATG GTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAAC GAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACC AAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCT AGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCC ACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGC GCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGG ATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAA ATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGC CTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGT GTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAA CGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACC TACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATC CGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCT GGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGAT GCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCC TGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGG ATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGC GCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCG CGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCA GTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACT TTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAA ACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGC TGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCCTGC AGCCCGGGG" } { name "pBSGUS" type "DNA" longname synthetic construct sequence-ID "pBSGUS" descrip "pBSGUS - GUS gene on BamHI/SacI fragment from pBI101 cloned into" creator "Fristensky,B." creation-date 1/17/100 22:57: 0 circular 1 direction 1 strandedness 1 comments " pBluescriptKSm13+.KEYWORDS . SOURCE synthetic construct. artificial sequence. REFERENCE 1 (bases 1 to 4809) TITLE Untitled JOURNAL Unpublished REFERENCE 2 (bases 1 to 4809) AUTHORS Fristensky,B. TITLE Direct Submission JOURNAL Submitted (17-JAN-2000) Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada FEATURES Location/Qualifiers source 1..4809 /organism=`synthetic construct` source 1..1831 /organism=`synthetic construct` /plasmid=`pBI101` /note=`U12639:2528..4418` source 1833..4809 /organism=`synthetic construct` /plasmid=`` /note=`U52331:complement(653..694)` BASE COUNT 1200 a 1201 c 1249 g 1159 t " sequence " gatccccgggtggtcagtcccttatgttacgtcctgtagaaaccccaacccgtgaaatca aaaaactcgacggcctgtgggcattcagtctggatcgcgaaaactgtggaattgatcagc gttggtgggaaagcgcgttacaagaaagccgggcaattgctgtgccaggcagttttaacg atcagttcgccgatgcagatattcgtaattatgcgggcaacgtctggtatcagcgcgaag tctttataccgaaaggttgggcaggccagcgtatcgtgctgcgtttcgatgcggtcactc attacggcaaagtgtgggtcaataatcaggaagtgatggagcatcagggcggctatacgc catttgaagccgatgtcacgccgtatgttattgccgggaaaagtgtacgtatcaccgttt gtgtgaacaacgaactgaactggcagactatcccgccgggaatggtgattaccgacgaaa acggcaagaaaaagcagtcttacttccatgatttctttaactatgccggaatccatcgca gcgtaatgctctacaccacgccgaacacctgggtggacgatatcaccgtggtgacgcatg tcgcgcaagactgtaaccacgcgtctgttgactggcaggtggtggccaatggtgatgtca gcgttgaactgcgtgatgcggatcaacaggtggttgcaactggacaaggcactagcggga ctttgcaagtggtgaatccgcacctctggcaaccgggtgaaggttatctctatgaactgt gcgtcacagccaaaagccagacagagtgtgatatctacccgcttcgcgtcggcatccggt cagtggcagtgaagggccaacagttcctgattaaccacaaaccgttctactttactggct ttggtcgtcatgaagatgcggacttacgtggcaaaggattcgataacgtgctgatggtgc acgaccacgcattaatggactggattggggccaactcctaccgtacctcgcattaccctt acgctgaagagatgctcgactgggcagatgaacatggcatcgtggtgattgatgaaactg ctgctgtcggctttaacctctctttaggcattggtttcgaagcgggcaacaagccgaaag aactgtacagcgaagaggcagtcaacggggaaactcagcaagcgcacttacaggcgatta aagagctgatagcgcgtgacaaaaaccacccaagcgtggtgatgtggagtattgccaacg aaccggatacccgtccgcaagtgcacgggaatatttcgccactggcggaagcaacgcgta aactcgacccgacgcgtccgatcacctgcgtcaatgtaatgttctgcgacgctcacaccg ataccatcagcgatctctttgatgtgctgtgcctgaaccgttattacggatggtatgtcc aaagcggcgatttggaaacggcagagaaggtactggaaaaagaacttctggcctggcagg agaaactgcatcagccgattatcatcaccgaatacggcgtggatacgttagccgggctgc actcaatgtacaccgacatgtggagtgaagagtatcagtgtgcatggctggatatgtatc accgcgtctttgatcgcgtcagcgccgtcgtcggtgaacaggtatggaatttcgccgatt ttgcgacctcgcaaggcatattgcgcgttggcggtaacaagaaagggatcttcactcgcg accgcaaaccgaagtcggcggcttttctgctgcaaaaacgctggactggcatgaacttcg gtgaaaaaccgcagcagggaggcaaacaatgaatcaacaactctcctggcgcaccatcgt cggctacagcctcgggaattgctaccgagctccaattcgccctatagtgagtcgtattac aattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaactt aatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcacc gatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacgcgccctgtagcggc gcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgcc ctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccc cgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctc gaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacg gtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaact ggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatt tcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaa atattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctattt gtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaa tgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccctta ttcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaag taaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaaca gcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcactttta aagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtc gccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatc ttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataaca ctgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgc acaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagcca taccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaac tattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggagg cggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctg ataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatg gtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaac gaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagacc aagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatct aggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttcc actgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgc gcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccgg atcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaa atactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgc ctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgt gtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaa cggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacc tacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatc cggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcct ggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgat gctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcc tggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtgg ataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagc gcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccg cgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca gtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacact ttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaa acagctatgaccatgattacgccaagctcgaaattaaccctcactaaagggaacaaaagc tgggtaccgggccccccctcgaggtcgacggtatcgataagcttgatatcgaattcctgc agcccgggg" }