LOCUS NZ_ABVG02000010 18386 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum JW20 ctg271, whole genome shotgun sequence. ACCESSION NZ_ABVG02000010 NZ_ABVG00000000 VERSION NZ_ABVG02000010.1 GI:281419553 KEYWORDS WGS. SOURCE Clostridium thermocellum JW20 ORGANISM Clostridium thermocellum JW20 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ABVG02000010. FEATURES Location/Qualifiers source 1..18386 /organism="Clostridium thermocellum JW20" /mol_type="genomic DNA" /strain="JW20" /isolation_source="cotton bale" /db_xref="taxon:492476" /country="USA: Louisiana" gene complement(<1..358) /locus_tag="Cther_0048" CDS complement(<1..358) /locus_tag="Cther_0048" /EC_number="2.7.7.6" /inference="protein motif:PRIAM:2.7.7.6" /note="PFAM: RNA polymerase Rpb1 domain 1; KEGG: cce:Ccel_0313 DNA-directed RNA polymerase, beta' subunit" /codon_start=1 /transl_table=11 /product="DNA-directed RNA polymerase" /protein_id="ZP_06250565.1" /db_xref="GI:281419554" /db_xref="InterPro:IPR007080" /translation="MFELNNLDSIRIGLASPEKIREWSRGEVKKPETINYRTLKPERD GLFCERIFGPQKDWECHCGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS HIWYFKGIPSRMGLLLD" misc_feature complement(<1..349) /locus_tag="Cther_0048" /note="RNA polymerase Rpb1, domain 1; Region: RNA_pol_Rpb1_1; pfam04997" /db_xref="CDD:147265" gene complement(376..4128) /locus_tag="Cther_0049" CDS complement(376..4128) /locus_tag="Cther_0049" /EC_number="2.7.7.6" /inference="protein motif:TFAM:TIGR02013" /note="KEGG: cce:Ccel_0312 DNA-directed RNA polymerase, beta subunit; TIGRFAM: DNA-directed RNA polymerase, beta subunit; PFAM: RNA polymerase Rpb2 domain 6; RNA polymerase beta subunit; RNA polymerase Rpb2 domain 2; RNA polymerase Rpb2 domain 3; DNA-directed RNA polymerase, beta subunit, external 1 domain; RNA polymerase Rpb2 domain 7" /codon_start=1 /transl_table=11 /product="DNA-directed RNA polymerase, beta subunit" /protein_id="ZP_06250566.1" /db_xref="GI:281419555" /db_xref="InterPro:IPR007120" /db_xref="InterPro:IPR007121" /db_xref="InterPro:IPR007641" /db_xref="InterPro:IPR007642" /db_xref="InterPro:IPR007644" /db_xref="InterPro:IPR007645" /db_xref="InterPro:IPR010243" /db_xref="InterPro:IPR019462" /translation="MVHPVKLGRNVRMSYSKIDEVIDMPNLIEIQKNSYEQFLKEGFK EVFKDVNPITDYTGNLILEFVDYSLDEPPKYSVDECKERDATYAAPLKVKVRLINKET GEVKEQEIFMGDFPLMTETGTFIINGAERVIVSQLVRSPGIYYAMKIDKAGKQLFSNT VIPNRGAWLEYETDSNDVLSVRIDRTRKLPLTVLVRALGYGTDLEITELFGEDERILA TIQKDSTKTEEEGLLEIYKRLRPGEPPTVESAKALLHGLFFDPKRYDLAKPGRFKFNK KLSIAARIHGFIAGENIKDPDTGEIIVAEGETISREKAETIQNAGVNTVILRVDGKNV KVIGNDMVDIKRYVDFDPKEIGINEKVKRDVLMEILEEYKGKGDDAIKKALQERIDDL IPKHITKEDIISSISYIIGLSYGIGSTDDIDHLGNRRLRSVGELLQNQFRIGLSRMER VVRERMTIQDLDVATPQALINIRPVAAAIKEFFGSSQLSQFMDQTNPLAELTHKRRLS ALGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLIGSLSTYARVNEYGFIET PYRKVSKEEPGKVTNEIVYLTADEEDEYIIAQANEPLDEEGRFISNKVVCRYKEEFIE VDPSKIDFMDVSPKQIVSVATSMIPFLENDDANRALMGANMQRQAVPLIKTESPIVGT GIEYRAARDSGVVILAKNPGVVEKVTANEIIIRTKDGKRDTYKLLKYMRSNQGTCINQ RPIVKKGEEVEAGDVIADGPSTDNGEIALGKNVLVGFMTWEGYNYEDAILISERLVKD DVFTSIHIEEYEAEARDTKLGPEDITREIPNVSEDALKDLNSEGIIRIGAEVRAGDIL VGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLRVPHGESGIVVDVKIFTRENGDE LAPGVNKLVRVYVAQKRKISVGDKMAGRHGNKGVISRILPVEDMPFLPDGTPLDIVLN PLGVPSRMNIGQVLEVHLGYAAKALGWKVATPVFDGATEEDIVQTLRKAGLAEDGKSI LYDGRTGEPFENRVTVGYMYMLKLAHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQR FGEMEVWALEAYGAAYTLQEILTVKSDDVVGRVKTYEAIVKGENVPEPGIPESFKVLI KELQSLCLDVKVYSEEQEEIAIKESVEDDLEELNVNIEGREDEVNFNEFNDIGEEITD EDLEVEDFDLQDLTDDDINLDDTIDAEPDDNLFDDDFDDTFDDDDL" misc_feature complement(634..4128) /locus_tag="Cther_0049" /note="DNA-directed RNA polymerase subunit beta; Reviewed; Region: rpoB; PRK00405" /db_xref="CDD:179007" misc_feature complement(<3565..4050) /locus_tag="Cther_0049" /note="RNA polymerase beta subunit. RNA polymerases catalyse the DNA dependent polymerization of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). Each RNA polymerase...; Region: RNA_pol_B_RPB2; cl04593" /db_xref="CDD:156170" misc_feature complement(<3367..3714) /locus_tag="Cther_0049" /note="RNA polymerase beta subunit; Region: RNA_pol_Rpb2_1; cl04591" /db_xref="CDD:123212" misc_feature complement(<1861..>2871) /locus_tag="Cther_0049" /note="RNA polymerase beta subunit. RNA polymerases catalyse the DNA dependent polymerization of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). Each RNA polymerase...; Region: RNA_pol_B_RPB2; cd00653" /db_xref="CDD:73223" misc_feature complement(652..>2265) /locus_tag="Cther_0049" /note="RNA polymerase beta subunit. RNA polymerases catalyse the DNA dependent polymerization of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). Each RNA polymerase...; Region: RNA_pol_B_RPB2; cd00653" /db_xref="CDD:73223" misc_feature complement(order(652..654,658..660,664..675,679..681, 685..690,694..696,700..702,712..714,781..783,793..810, 814..831,835..837,841..846,850..852,862..870,931..936, 940..948,955..957,994..996,1000..1008,1030..1032, 1111..1113,1162..1164,1174..1176,1189..1191,1198..1200, 1207..1209,1213..1215,1279..1281,1285..1287,1291..1293, 1768..1776,1780..1782,1789..1800,2161..2163,2167..2175, 2227..2229)) /locus_tag="Cther_0049" /note="RPB1 interaction site; other site" /db_xref="CDD:73223" misc_feature complement(order(1882..1884,1888..1896,1906..1908, 1912..1914,1999..2001)) /locus_tag="Cther_0049" /note="RPB12 interaction site; other site" /db_xref="CDD:73223" misc_feature complement(order(991..993,1042..1044,1135..1137, 1141..1146,1225..1227,1264..1266,1735..1737,1744..1746, 1807..1809,1813..1815,1906..1911)) /locus_tag="Cther_0049" /note="RPB10 interaction site; other site" /db_xref="CDD:73223" misc_feature complement(order(1024..1026,1267..1269,1738..1740, 1780..1782)) /locus_tag="Cther_0049" /note="RPB11 interaction site; other site" /db_xref="CDD:73223" misc_feature complement(order(1000..1002,1006..1029,1240..1245, 1249..1251,1258..1266,1339..1341,1345..1347,1723..1725, 1735..1740)) /locus_tag="Cther_0049" /note="RPB3 interaction site; other site" /db_xref="CDD:73223" misc_feature complement(order(1582..1584,1588..1596,1606..1608, 1612..1614,1723..1725)) /locus_tag="Cther_0049" /note="RPB12 interaction site; other site" /db_xref="CDD:73223" gene complement(4627..5016) /locus_tag="Cther_0050" CDS complement(4627..5016) /locus_tag="Cther_0050" /inference="protein motif:TFAM:TIGR00855" /note="TIGRFAM: ribosomal protein L7/L12; PFAM: Ribosomal protein L7/L12; KEGG: cce:Ccel_0311 ribosomal protein L7/L12" /codon_start=1 /transl_table=11 /product="ribosomal protein L7/L12" /protein_id="ZP_06250567.1" /db_xref="GI:281419556" /db_xref="InterPro:IPR000206" /db_xref="InterPro:IPR008932" /db_xref="InterPro:IPR013823" /db_xref="InterPro:IPR014719" /translation="MASEKVTKLIEDVKALSVLELSELVKALEEEFGVSAAAPVAVAA VGAAAPGAAAPAAEEKTEFEVILKSPGADKIKVIKVVREVTGLGLKEAKDLVDGAPKT LKEGVSKDEAAQIEAKFKEVGATVEIK" misc_feature complement(4633..5007) /locus_tag="Cther_0050" /note="Ribosomal protein L7/L12. Ribosomal protein L7/L12 refers to the large ribosomal subunit proteins L7 and L12, which are identical except that L7 is acetylated at the N terminus. It is a component of the L7/L12 stalk, which is located at the surface of...; Region: Ribosomal_L7_L12; cd00387" /db_xref="CDD:100102" misc_feature complement(order(4684..4686,4693..4695,4699..4704, 4744..4749,4753..4755,4759..4764,4768..4770,4831..4836, 4861..4869,4873..4878,4885..4887,4930..4932,4939..4941, 4948..4953,4960..4968,5005..5007)) /locus_tag="Cther_0050" /note="core dimer interface; other site" /db_xref="CDD:100102" misc_feature complement(order(4921..4923,4936..4938,4975..4977, 4987..4989,4996..4998)) /locus_tag="Cther_0050" /note="peripheral dimer interface; other site" /db_xref="CDD:100102" misc_feature complement(order(4921..4923,4930..4932,4942..4947, 4954..4956)) /locus_tag="Cther_0050" /note="L10 interface; other site" /db_xref="CDD:100102" misc_feature complement(order(4738..4740,4747..4752,4771..4773, 4780..4785,4792..4797)) /locus_tag="Cther_0050" /note="L11 interface; other site" /db_xref="CDD:100102" misc_feature complement(order(4738..4740,4747..4752,4771..4773, 4780..4782,4792..4794)) /locus_tag="Cther_0050" /note="putative EF-Tu interaction site; other site" /db_xref="CDD:100102" misc_feature complement(order(4771..4773,4780..4785,4792..4794)) /locus_tag="Cther_0050" /note="putative EF-G interaction site; other site" /db_xref="CDD:100102" gene complement(5104..5640) /locus_tag="Cther_0051" CDS complement(5104..5640) /locus_tag="Cther_0051" /inference="protein motif:PFAM:PF00466" /note="PFAM: ribosomal protein L10; KEGG: cce:Ccel_0310 ribosomal protein L10" /codon_start=1 /transl_table=11 /product="ribosomal protein L10" /protein_id="ZP_06250568.1" /db_xref="GI:281419557" /db_xref="InterPro:IPR001790" /db_xref="InterPro:IPR002363" /translation="MPSEKVLQQKKEIVRQLSENMKSAKTIVFADYRGLTVEQDTELR NKLRKAGVEYKVVKNTLTKLAAKENGLEELEPYFNGPTSMAFSDKDVVAPAKIMVEYA EKYENLELKVGVVEGKVCDLKTLKAIAALPPKEELVAKALGSLKSPIAGLVNVLNGNI RGLVIALNAIAEKKKESA" misc_feature complement(5158..5622) /locus_tag="Cther_0051" /note="Ribosomal protein L10 family, L10 subfamily; composed of bacterial 50S ribosomal protein and eukaryotic mitochondrial 39S ribosomal protein, L10. L10 occupies the L7/L12 stalk of the ribosome. The N-terminal domain (NTD) of L10 interacts with L11...; Region: Ribosomal_L10; cd05797" /db_xref="CDD:88597" misc_feature complement(order(5449..5451,5458..5469,5599..5601, 5608..5613)) /locus_tag="Cther_0051" /note="23S rRNA interface; other site" /db_xref="CDD:88597" misc_feature complement(order(5161..5166,5170..5175,5182..5190, 5194..5211,5215..5220,5227..5232,5239..5241,5287..5289, 5296..5298,5365..5367)) /locus_tag="Cther_0051" /note="Interface with L7/L12 ribosomal proteins; other site" /db_xref="CDD:88597" gene complement(5913..6608) /locus_tag="Cther_0052" CDS complement(5913..6608) /locus_tag="Cther_0052" /inference="protein motif:TFAM:TIGR01169" /note="TIGRFAM: ribosomal protein L1; PFAM: ribosomal protein L1; KEGG: cce:Ccel_0309 ribosomal protein L1" /codon_start=1 /transl_table=11 /product="ribosomal protein L1" /protein_id="ZP_06250569.1" /db_xref="GI:281419558" /db_xref="InterPro:IPR002143" /db_xref="InterPro:IPR005878" /db_xref="InterPro:IPR016094" /translation="MKRGKKYQESVKLVDKTKLYDPVEAMELVQKTAKAKFDETVEAH IRLGVDSRHADQQVRGAVVLPHGTGKKVRVLVFAKGEKATEAEKAGADYVGAEELVSK IQNENWFEFDVVVATPDMMGVVGRLGKVLGPKGLMPNPKAGTVTMDVAKAIADIKAGK IEYRLDKTNIIHCPIGKVSFGTEKLVDNFRTLMSAIIKAKPAAAKGQYLKSVVVTSTM GPGIKVNPLRVSE" misc_feature complement(5937..6539) /locus_tag="Cther_0052" /note="Ribosomal protein L1. The L1 protein, located near the E-site of the ribosome, forms part of the L1 stalk along with 23S rRNA. In bacteria and archaea, L1 functions both as a ribosomal protein that binds rRNA, and as a translation repressor that...; Region: Ribosomal_L1; cd00403" /db_xref="CDD:88601" misc_feature complement(order(5946..5948,5952..5957,6093..6095, 6099..6101,6105..6107,6471..6473,6477..6479,6483..6485, 6489..6491,6498..6506)) /locus_tag="Cther_0052" /note="mRNA/rRNA interface; other site" /db_xref="CDD:88601" gene complement(6709..7134) /locus_tag="Cther_0053" CDS complement(6709..7134) /locus_tag="Cther_0053" /inference="protein motif:TFAM:TIGR01632" /note="KEGG: cce:Ccel_0308 ribosomal protein L11; TIGRFAM: ribosomal protein L11; PFAM: Ribosomal protein L11-like; SMART: ribosomal protein L11" /codon_start=1 /transl_table=11 /product="ribosomal protein L11" /protein_id="ZP_06250570.1" /db_xref="GI:281419559" /db_xref="InterPro:IPR000911" /db_xref="InterPro:IPR006519" /db_xref="InterPro:IPR020783" /db_xref="InterPro:IPR020784" /translation="MAKKVVGYIKLQIPAGKATPAPPVGPALGQHGVNIMAFCKEFNE RTAKDAGLVIPVVITVYADRSFSFVTKTPPASVLIKRACKIEKGSGRPNREKVAKISK EEVRKIAEMKMPDLNAASLEAAMSMIAGTARSMGVVVED" misc_feature complement(6712..7134) /locus_tag="Cther_0053" /note="50S ribosomal protein L11; Validated; Region: rplK; PRK00140" /db_xref="CDD:178895" misc_feature complement(6718..7110) /locus_tag="Cther_0053" /note="Ribosomal protein L11. Ribosomal protein L11, together with proteins L10 and L7/L12, and 23S rRNA, form the L7/L12 stalk on the surface of the large subunit of the ribosome. The homologous eukaryotic cytoplasmic protein is also called 60S ribosomal...; Region: Ribosomal_L11; cd00349" /db_xref="CDD:100101" misc_feature complement(order(6730..6738,6742..6747,6754..6759, 6766..6768,6778..6786,6799..6801,6874..6876,6895..6897, 6907..6915,7045..7047,7105..7107)) /locus_tag="Cther_0053" /note="23S rRNA interface; other site" /db_xref="CDD:100101" misc_feature complement(order(6781..6786,6793..6798,6928..6933, 6937..6939,6949..6960,6964..6966,7105..7107)) /locus_tag="Cther_0053" /note="L7/L12 interface; other site" /db_xref="CDD:100101" misc_feature complement(order(7045..7047,7057..7059)) /locus_tag="Cther_0053" /note="putative thiostrepton binding site; other site" /db_xref="CDD:100101" misc_feature complement(order(6847..6849,6856..6858)) /locus_tag="Cther_0053" /note="L25 interface; other site" /db_xref="CDD:100101" gene complement(7276..7809) /locus_tag="Cther_0054" CDS complement(7276..7809) /locus_tag="Cther_0054" /inference="protein motif:TFAM:TIGR00922" /note="KEGG: cce:Ccel_0307 NusG antitermination factor; TIGRFAM: transcription termination/antitermination factor NusG; PFAM: NGN domain protein; KOW domain protein; SMART: NGN domain protein; KOW domain protein" /codon_start=1 /transl_table=11 /product="NusG antitermination factor" /protein_id="ZP_06250571.1" /db_xref="GI:281419560" /db_xref="InterPro:IPR001062" /db_xref="InterPro:IPR005824" /db_xref="InterPro:IPR006645" /db_xref="InterPro:IPR008991" /db_xref="InterPro:IPR014722" /db_xref="InterPro:IPR015869" /translation="MPNNEASEEAKWYVVHTYSGYENKVKANLEKIVENRNLQDYILD IVVPMEEQIEIKDGKKKASLKKVFPGYVLVKMIMNDESWYVVRNTRGVTGFVGPGSKA VPLTDEEIRAMGVEEFAPVLDYEVGDNVRVVSGPLENFIGIVEEINLEKKKVRVSVSM FGRETPVELELHQIQKI" misc_feature complement(7279..7797) /locus_tag="Cther_0054" /note="transcription antitermination protein NusG; Validated; Region: nusG; PRK05609" /db_xref="CDD:180161" misc_feature complement(7471..7779) /locus_tag="Cther_0054" /note="Transcription termination factor nusG; Region: NusG; cl02766" /db_xref="CDD:155092" gene complement(7865..8107) /locus_tag="Cther_0055" CDS complement(7865..8107) /locus_tag="Cther_0055" /inference="protein motif:TFAM:TIGR00964" /note="TIGRFAM: preprotein translocase, SecE subunit; PFAM: protein secE/sec61-gamma protein; KEGG: cce:Ccel_0306 preprotein translocase, SecE subunit" /codon_start=1 /transl_table=11 /product="preprotein translocase, SecE subunit" /protein_id="ZP_06250572.1" /db_xref="GI:281419561" /db_xref="InterPro:IPR001901" /db_xref="InterPro:IPR005807" /translation="MAEEVKVSKVEKKKEGFLKRAAGKFSKYFKDVKNELKRVIWPTR SQLINNTITVLILCFIVGVLIWLFDLGFGALSSLIY" misc_feature complement(7871..8038) /locus_tag="Cther_0055" /note="SecE/Sec61-gamma subunits of protein translocation complex; Region: SecE; cl00481" /db_xref="CDD:186025" gene complement(8196..8345) /locus_tag="Cther_0056" CDS complement(8196..8345) /locus_tag="Cther_0056" /inference="protein motif:TFAM:TIGR01023" /note="TIGRFAM: ribosomal protein L33; PFAM: ribosomal protein L33; KEGG: cac:CAC3151 50S ribosomal protein L33" /codon_start=1 /transl_table=11 /product="ribosomal protein L33" /protein_id="ZP_06250573.1" /db_xref="GI:281419562" /db_xref="InterPro:IPR001705" /db_xref="InterPro:IPR018264" /translation="MRVKITLACGECKQRNYNTMKNKKNDPDRLEMRKYCRFCQKHTM HKETK" misc_feature complement(8199..8345) /locus_tag="Cther_0056" /note="Ribosomal protein L33; Region: Ribosomal_L33; cl00383" /db_xref="CDD:185956" mobile_element 8527..9912 /mobile_element_type="insertion sequence:ISCth3 element like, family IS30" gene 8640..9710 /locus_tag="Cther_0057" CDS 8640..9710 /locus_tag="Cther_0057" /inference="protein motif:PFAM:PF00665" /note="PFAM: Integrase catalytic region; Trp repressor; KEGG: tex:Teth514_2303 integrase catalytic subunit" /codon_start=1 /transl_table=11 /product="Integrase catalytic region" /protein_id="ZP_06250574.1" /db_xref="GI:281419563" /db_xref="InterPro:IPR000831" /db_xref="InterPro:IPR001584" /db_xref="InterPro:IPR009057" /db_xref="InterPro:IPR012337" /translation="MAVQYKSTTTEHKFKHLSVYERGQIAALLKEGKSQRYIANKLGR SPSTISREIKRGTTMQMRTDLSTYKVYFPETGQAVYEKNRMNCGAKRKLAQVEDFLKF AEDKILREKWSPDAVVGLCRRDPKWQNSTIVCTKTLYNYIDLGLIKVRNIDLNLKLRL KSKIKRIRQNKRVVGKSIDQRPEEVQSRQTFGHWEIDTVTGKKSNDSVILTLTERKTR YELLFLLDAKDSNTVNEALSELKNCYGKDVSNVFRTITADNGSEFSRLSEMLQGLGIE AYFTHPYSSWERGTNERHNGLIRRFIPKGKAIKDFSEETIKRIQQWLNSLPRRILGYK TPEECFNEEIHNLVNKNISAIA" misc_feature 8676..9680 /locus_tag="Cther_0057" /note="Transposase and inactivated derivatives, IS30 family [DNA replication, recombination, and repair]; Region: Tra8; COG2826" /db_xref="CDD:32654" misc_feature 9195..9545 /locus_tag="Cther_0057" /note="Integrase core domain; Region: rve; cl01316" /db_xref="CDD:154329" gene complement(10019..11686) /locus_tag="Cther_0058" CDS complement(10019..11686) /locus_tag="Cther_0058" /inference="protein motif:TFAM:TIGR00118" /note="TIGRFAM: acetolactate synthase, large subunit, biosynthetic type; PFAM: thiamine pyrophosphate protein TPP binding domain protein; thiamine pyrophosphate protein central region; thiamine pyrophosphate protein domain protein TPP-binding; KEGG: cce:Ccel_0303 acetolactate synthase, large subunit, biosynthetic type" /codon_start=1 /transl_table=11 /product="acetolactate synthase, large subunit, biosynthetic type" /protein_id="ZP_06250575.1" /db_xref="GI:281419564" /db_xref="InterPro:IPR000399" /db_xref="InterPro:IPR011766" /db_xref="InterPro:IPR012000" /db_xref="InterPro:IPR012001" /db_xref="InterPro:IPR012846" /translation="MKLTGSKILIECLKEQGVDTIFGFPGGAVLNIYDELYRSQNEIR HILTSHEQGAAHAADGYARATGKVGVCLATSGPGATNLVTGIATAYMDSVPMVAITGQ VATPLLGKDSFQEVDITGITMPITKHNFIVKDVNKLADIVRRAFYIAKEGRPGPVLID ICKDVTAAYAEYEPKSPQELPEVPVRVDEKCIDEAAEAINKAERPVILAGGGVSIAGA NKELFEFATNAQIPVTTTLMGMGAFPGTHELFMGMIGMHGTKTTNMAVSESDLFIAIG ARFSDRVISNVQRFAPKASIMHIDIDPAEIGKNINVQYALEGNIKKILQLLNERVKKK ECTDWVRKINEWKELYPLKYPQDDKLHPQYIIERMYELTKGEAIITTEVGQHQMWAAQ FYKYTSPRQFLSSGGLGTMGYGLGACIGARIGRPDKKVINVAGDGSFRMNCNELATAV EYKLPIIVAIFNNHALGMVRQWQQLFYGGRYSSTSLDRCTDFKALAEAYGAIGINVTA KEEVDEALNRALASEDTPVVINFEIDKDEMVFPIVPPGAPLSELIEE" misc_feature complement(10022..11683) /locus_tag="Cther_0058" /note="acetolactate synthase 3 catalytic subunit; Reviewed; Region: PRK06048" /db_xref="CDD:180368" misc_feature complement(11204..11665) /locus_tag="Cther_0058" /note="Pyrimidine (PYR) binding domain of POX and related proteins; Region: TPP_PYR_POX_like; cd07035" /db_xref="CDD:132918" misc_feature complement(order(11411..11416,11423..11425,11435..11437, 11456..11458,11492..11500,11504..11509,11516..11521, 11528..11530,11537..11557,11585..11587,11597..11599, 11609..11614,11627..11629)) /locus_tag="Cther_0058" /note="PYR/PP interface; other site" /db_xref="CDD:132918" misc_feature complement(order(11315..11317,11324..11326,11426..11428, 11435..11440,11444..11449,11456..11458,11531..11533, 11537..11548,11552..11554,11585..11587,11597..11599, 11606..11614)) /locus_tag="Cther_0058" /note="dimer interface; other site" /db_xref="CDD:132918" misc_feature complement(order(11444..11446,11456..11458,11534..11536, 11612..11614)) /locus_tag="Cther_0058" /note="TPP binding site; other site" /db_xref="CDD:132918" misc_feature complement(10706..11113) /locus_tag="Cther_0058" /note="Thiamine pyrophosphate enzyme, central domain; Region: TPP_enzyme_M; pfam00205" /db_xref="CDD:143962" misc_feature complement(10049..10609) /locus_tag="Cther_0058" /note="Thiamine pyrophosphate (TPP) family, Acetohydroxyacid synthase (AHAS) subfamily, TPP-binding module; composed of proteins similar to the large catalytic subunit of AHAS. AHAS catalyzes the condensation of two molecules of pyruvate to give the...; Region: TPP_AHAS; cd02015" /db_xref="CDD:48178" misc_feature complement(order(10298..10300,10364..10366,10373..10381, 10454..10456,10460..10462,10529..10540)) /locus_tag="Cther_0058" /note="TPP-binding site; other site" /db_xref="CDD:48178" misc_feature complement(order(10193..10195,10202..10204,10214..10219, 10334..10336,10355..10360,10364..10369,10454..10456, 10460..10462,10469..10471)) /locus_tag="Cther_0058" /note="dimer interface; other site" /db_xref="CDD:48178" gene complement(11755..13419) /locus_tag="Cther_0059" CDS complement(11755..13419) /locus_tag="Cther_0059" /EC_number="4.2.1.9" /inference="protein motif:TFAM:TIGR00110" /note="KEGG: cce:Ccel_0302 dihydroxy-acid dehydratase; TIGRFAM: dihydroxy-acid dehydratase; PFAM: dihydroxy-acid and 6-phosphogluconate dehydratase" /codon_start=1 /transl_table=11 /product="dihydroxy-acid dehydratase" /protein_id="ZP_06250576.1" /db_xref="GI:281419565" /db_xref="InterPro:IPR000581" /db_xref="InterPro:IPR004404" /db_xref="InterPro:IPR020558" /translation="MRSDAVKKGIERAPHRALFKAMGYTDEELERPLIGVVNSRNEIV PGHIHLDKIAEAVKAGIRMAGGTPVEFGAIGVCDGIAMGHTGMKYSLATRELIADSCE AMALAHSFDGMVFIPNCDKIVPGMLMAAARINVPAIVVSGGPMLSLRHNDKNLDLNSV FEAVGAYKAGKMTEKEVWEYEEKACPGCGSCSGMFTANSMNCLTEVLGMGLPGNGTVP AVYAERIRLAKKAGMKIVELVEKDIKPSDILTPKAFENALAVDMALGCSTNSVLHLPA IANEVGIEINLDIINEISSKVPNLCKLAPAGHHHVQDLYAAGGIPAVMKELSKKNLLH LDLITVTGKTVRENIENAKVRDYEVIRSIDNPYSPTGGIAVLRGNLAPDGAVVKRSAV APEMLVHKGPARVFDSEDAAIEAIYNGKINKGDVVIIRYEGPKGGPGMREMLSPTSAI AGMGLDKDVALITDGRFSGATRGASIGHVSPEAMAGGPIAIVRDGDIISIDIPNGKLD VEIPDSEIQKRLKEWKAPAPKITKGYLGRYAKLVSSANKGAILENK" misc_feature complement(11764..13419) /locus_tag="Cther_0059" /note="Dehydratase family; Region: ILVD_EDD; cl00340" /db_xref="CDD:185921" misc_feature complement(11767..13359) /locus_tag="Cther_0059" /note="dihydroxy-acid dehydratase; Region: ilvD; TIGR00110" /db_xref="CDD:161713" gene complement(13691..14410) /locus_tag="Cther_0060" CDS complement(13691..14410) /locus_tag="Cther_0060" /inference="protein motif:PFAM:PF02674" /note="PFAM: Colicin V production protein; KEGG: cce:Ccel_0301 hypothetical protein" /codon_start=1 /transl_table=11 /product="Colicin V production protein" /protein_id="ZP_06250577.1" /db_xref="GI:281419566" /db_xref="InterPro:IPR003825" /translation="MNWIDLVVIAIILVLAWIGLKKGIVYSVFKLASFFISAVLSAKL YPIIAKILSNSQVFHSIKKGIYHNLMLRHEALSLPFDAAAKATAQSVVDGLSLPGFMK GMIKNSLLKSLPSLTELIDVSTIMDSLSDVLAHMIVDIISLIVMFVAVRVGLFVLERV LKGVTSLPIVKQADKAGGFILGALEGLLTVYIVFAILIMFSASPKFQGVFEAIESSTV AKVLYHNNFIVDWMFPKDVIV" misc_feature complement(13745..>14023) /locus_tag="Cther_0060" /note="Colicin V production protein; Region: Colicin_V; cl00567" /db_xref="CDD:186085" gene complement(14428..15609) /locus_tag="Cther_0061" CDS complement(14428..15609) /locus_tag="Cther_0061" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: cce:Ccel_0300 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_06250578.1" /db_xref="GI:281419567" /translation="MNSPENIPKMRQPSSRMSILLFFVLVIIVVCTAAVAYLNSKGID IKSVSIRDIIANGFFVGDKDVYEVTGTLLRYEDSLNSEFGAYKGYIVRCTKNGLEYLN RNGEEEWIYTVSLNLPYFKSAGEYLLVAGLESKDIYVFAGKEKKWEKKLDYSIINANI NSAGYVTVLHKAERDKSAVSVYNKQGVFLFTKILGETYAISSEVSPSGREVLINCVDI SGVSINTGLHLYTISGGNVAGKTFENVIFPSIRYLNDDTIVAVSDSAIYLFGQNFEEK WSRTISGKVYSMDVMKGRYAVFAFSEKGMMGEAGHVLIVDSKGREVADYRIDQDVVNI AANDDFIAINTSKCVYFIDVAGRLRGSYASDYLINEVKFLGTNEALAITKEGVILLKM N" gene complement(15690..16799) /locus_tag="Cther_0062" CDS complement(15690..16799) /locus_tag="Cther_0062" /inference="similar to AA sequence:KEGG:Ccel_0299" /note="KEGG: cce:Ccel_0299 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_06250579.1" /db_xref="GI:281419568" /translation="MSQDVKNILKVASIYMATVIGAGFASGQEIMQFFSSYYEGGFYG IVLAGVLFALIGYIVLVKVYSERIRNYEEFLFPSVGWVIGWIMEIMVTLFMGSVLCIM VAGAGSIISGALNIPYHYGTLIAAILCMIVFLTDIKGIVALSSFVTPILVLGILGAGF YVIVNMDAWVFSPVPGFDAITDNWLVSSLLYVSYNSISGVAVMCSLLPYLKSRKIAAA GGIIGGLALSFIAIVLNIILYVFYPDIVSEEIPVLSIIGRHNFILGEFYKILLLLAMF ISAVTSGYGFIERIANKVRISRKILIPVICGFVVPISNVGFSGLISSVYPAFGYVGMF MLFMILIQGLNMLVSKLAASFAFVSSKREYKNRDR" misc_feature complement(15765..16784) /locus_tag="Cther_0062" /note="Uncharacterized membrane protein [Function unknown]; Region: COG3949" /db_xref="CDD:33730" gene complement(16877..18355) /locus_tag="Cther_0063" CDS complement(16877..18355) /locus_tag="Cther_0063" /inference="similar to AA sequence:KEGG:Ccel_0298" /note="KEGG: cce:Ccel_0298 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_06250580.1" /db_xref="GI:281419569" /translation="MRSLKLYRNIVILVVLFLGLIGAYFAIKNRLPAEEEDSYKQIEV YKLDKEKAEELTIVSGGKTFVLKKNDSEWQLVSGGDFEINKVQVESIVSNICDLYASK EVESDPKNLGQYGLDNPVTVTLKFSDGTVAEIEVGDQTPTGSGYYIREKGKNKVYTIA YYAGNILKANENSIRNRFVLDVTSQDVTELAVTKKGKRSFKLVKSDDKGWHMTEPIEA NANMVRLNTALEALVRAEVIDYIEKDVKDLSKYGLDNPSYVVEAAAGSKRVTLLIGDV KENNAESYGMFEGTNEVFTINPGTLGFLDTPALEMMDGFIYAPYIYTVTDLDFNIDGR TIKIKIEEVKDETQTQETDKSDESDTSGESEEGENEEEKKYKHYIDGIDVEAKKGEEG ISKLRNFYSTIIGITASEIEPDATPSGEAEISIVYHLNTEPGKVTVEFIPRDERTYYA VKNGKYTGIVVRKDAFDAEDGPRKTYEELMKFLNSEDEGKEE" ORIGIN 1 tgtccaaaag cagtcccatt ctgctgggta ttcctttaaa gtaccatatg tgggacacag 61 gggctgccag ctcaatatgc cccattcttt cccttcttac ttttgacctt gtaacttcaa 121 ctccacaacg gtcacacact atgcctttat atctgattct cttatacttt ccgcaatggc 181 actcccagtc cttttggggt ccgaatattc tttcacagaa aagtccgtct ctttcaggct 241 ttaacgttct gtagtttatg gtctcgggtt tttttacttc acccctggac cattctctga 301 tcttttccgg agaagctaaa cctattctta ttgaatccaa gttattaagt tcaaacacgg 361 ccttttcacc ttcccttata aatcatcgtc atcaaaggta tcatcaaaat catcgtcaaa 421 aagattgtca tccggttcgg catcaatagt gtcatcaaga ttgatatcat cgtcggttaa 481 atcctgcagg tcgaagtctt ccacttcaag gtcttcatcc gttatttcct ctccgatgtc 541 gttaaactca ttgaaattaa cttcatcttc cctgccctca atgttgacgt taagttcttc 601 aagatcatct tcaaccgatt ctttaatagc tatttcttcc tgttcctccg agtatacttt 661 cacatcaagg cacaaacttt gaagttcctt gataagaacc ttgaaggatt cggggatacc 721 cggctccgga acgttttcgc cctttactat tgcctcgtaa gtcttaaccc tgcccactac 781 atcgtccgac ttgaccgtca gtatctcctg cagtgtatag gcagcaccgt aagcttccaa 841 agcccaaact tccatctctc cgaatctctg tccgccgaat tgtgccttac ctcccaaagg 901 ctgctgcgtt accagagaat atggaccggt tgaacgggca tgtatcttgt catctacaag 961 gtgggcaagc ttgagcatat acatataacc tacagtcacc ctgttttcaa acggttctcc 1021 cgttcttccg tcatacaata ttgacttgcc gtcttcagca agacctgctt ttctcagcgt 1081 ctgaactata tcttcctcgg tagcaccgtc aaaaaccgga gttgcaactt tccagccaag 1141 ggctttagcc gcataaccaa gatgtacctc aagtacctgt ccgatattca tacgggacgg 1201 aacgcccagc ggatttaaaa ctatatccaa tggagtaccg tcaggaagga aaggcatatc 1261 ttccaccggc agaatcctcg atataacacc cttgtttccg tgtcttcctg ccattttgtc 1321 tccaacagat atttttctct tctgtgcaac ataaaccctt accagcttgt ttactccagg 1381 tgccagttca tcaccgtttt ctcttgtaaa tatttttacg tcaacaacaa tacccgattc 1441 cccgtgaggc acacgcaaag aggtgtccct gacttccctt gctttttctc cgaaaattgc 1501 acgaagaagt ctttcttcag cggtaagctc cgtctctcct tttggcgtaa cctttcctac 1561 gaggatatca ccggctctga cttcggctcc tattctgata ataccttcgc tgttcaggtc 1621 tttcaatgca tcttcgctga cattgggtat ttctctggtt atatcttcgg gaccgagctt 1681 cgtgtccctt gcctcagcct catactcttc tatatggatg gatgtaaaca catcatcttt 1741 aaccagtctt tcacttatca ggatggcgtc ctcgtagttg tatccttccc aggtcataaa 1801 tcctaccaga acattctttc ctaatgcaat ttcaccgtta tctgtggaag gaccgtccgc 1861 tattacatct cccgcttcaa cctcttcacc ttttttcact atcggtcttt ggttgataca 1921 tgttccctgg ttggagcgca tgtatttgag gagcttgtaa gtatctcttt taccgtcttt 1981 tgtacgaata ataatctcat tggcggttac cttttcaaca actcccggat ttttcgccag 2041 tataacaact cctgaatccc ttgcagccct gtactcaatt cccgttccga caatcggcga 2101 ctcagttttt ataagcggaa ccgcctgacg ctgcatgttt gctcccatca gtgcacggtt 2161 cgcatcatca ttttcaagga acggtatcat tgatgtcgca acagaaacta tctgctttgg 2221 tgatacatcc ataaagtcaa ttttggacgg atcaacctca ataaactctt ccttgtatct 2281 acatacaact ttattggaaa taaaccttcc ttcttcatcc agcggctcat ttgcctgcgc 2341 aataatatat tcgtcttcct catccgcagt caggtaaact atctcgttgg taactttacc 2401 cggttcttcc ttgcttactt ttctgtacgg cgtttcaata aaaccgtatt catttactct 2461 tgcataggta ctgagcgaac cgataagacc aatgttcggt ccttccggtg tctctatagg 2521 gcacatacgc ccatagtggg aatggtgaac gtcacgaact tcaaatcccg ctctttccct 2581 gctcaaacct ccgggaccca gcgcactgag ccttctttta tgcgtaagct ccgccaaagg 2641 attggtctgg tccatgaact gggacagctg gctgcttcca aagaactctt ttattgccgc 2701 cgccaccggc cttatattaa taagcgcctg tggagtggca acatccaggt cctggattgt 2761 cattctttcc cttaccactc tttccattct ggaaagacca atcctgaact gattttgcag 2821 aagctctcct acagaacgga gtcttctgtt acccaaatgg tcaatgtcat ccgtgcttcc 2881 gattccgtag ctcaatccta ttatatagct gatggacgat attatatctt ctttcgtgat 2941 atgctttgga atcagatcgt caattctttc ctgtaaagcc tttttgatcg catcgtcgcc 3001 ctttcccttg tactcttcaa gaatctccat taaaacatct cttttgactt tttcattgat 3061 gccgatttcc ttcgggtcaa aatccacata tcttttgata tccaccatgt cattgccgat 3121 gactttgaca tttttgccgt caactctgag aattaccgta ttgacaccgg cattttgtat 3181 cgtttcggcc ttttcccttg aaatggtttc tccttcagca acaattattt caccggtatc 3241 agggtcttta atattttcac ctgctataaa gccgtgtatt cttgctgcaa tggaaagctt 3301 tttgttaaat ttaaatcttc ccggctttgc caaatcatac cttttagggt cgaaaaacag 3361 tccgtggaga agtgcttttg cactttcaac cgtcggcggc tctcccggtc tgagcctttt 3421 gtatatctca agaagtcctt cctcttctgt cttggtactg tctttctgta ttgtcgcaag 3481 tattctttca tcctcgccaa aaagctcggt aatctcaaga tccgttccat atcccagtgc 3541 tcttaccaat accgtcagag gcagcttcct ggttctgtct atacgcaccg acaaaacgtc 3601 gtttgaatcg gtttcatatt caagccatgc tcctctgttt ggaataactg tatttgaaaa 3661 caactgtttt cctgctttat caatcttcat tgcatagtaa attccgggtg atcttacaag 3721 ctgactgact ataaccctct cagctccatt aataataaaa gttcccgttt cagtcatcag 3781 tgggaaatca cccataaaaa tttcctgttc tttaacttcg cccgtttcct tgttgataag 3841 acgcaccttt acttttaagg gtgcagcata agtcgcgtct ctttctttac attcatccac 3901 actgtatttg gggggttcat ccaatgaata gtctacaaat tccagaatca gatttccggt 3961 gtaatcagtt atgggattga cgtctttgaa aacctcctta aaaccctctt tgaggaattg 4021 ctcgtacgaa ttcttctgaa tttcgataag gtttggcatg tcgataactt catcaatttt 4081 tgaataactc attctaacgt ttcttcccaa tttcacggga tgtaccatta aatatcacct 4141 catatttcag acttttgtct tagtgctcaa accaaataaa aaaatccgat tttttatgta 4201 taaaattttg ttgccgacca atcaacacaa tattttaaag cttttgcaat tatctataac 4261 aaacaccagt atatttttca gctatataca tatactaaaa atacttcaac tcacagacat 4321 taaaatatta ttaacaaata ttgtctaact tattccaata tgctataata aaaaagtaaa 4381 aaagtttatg tttaatcaga aaatattgga atagtgcaat gaaattataa cgcagtttat 4441 aattctaaca tagatggttc aacttgtcaa ctatttcatg gaattttatt ttcaataata 4501 ttgccacctc ttattttata ctttttttga aaaaacagtt tatatcttaa tataacagat 4561 agcaaaaagg ctccttccct caaggaacct ttttgctatc tgtcaatcaa cgtcacctat 4621 aatacattat tttatttcta cagttgctcc aacttctttg aacttagctt caatttgagc 4681 tgcttcgtct ttggatacgc cttccttcaa tgtcttcgga gctccgtcaa ccaagtcttt 4741 tgcttctttc aaaccaagac cggttacttc tctgacaacc tttataactt tgattttgtc 4801 agctcccgga gacttcagga ttacttcaaa ctctgtcttt tcttcagccg ccggagctgc 4861 cgcacccgga gctgctgctc ctactgccgc aactgccaca ggagctgctg ccgatacacc 4921 gaattcttct tcaagagctt ttaccaattc tgataattcc aatactgata atgccttaac 4981 atcctcaatt aattttgtaa ctttttcact agccattttt atatacctcc atcaaaatta 5041 ttattttttt aattaattta atttagaaat atttgattta agaatttgaa atttatgcaa 5101 caattaagca ctttccttct tcttctccgc aattgcgttg agagcaatga ccaatcctct 5161 gatgttgccg tttaatacat ttacaagacc cgctatcgga gacttcaagc ttccgagagc 5221 ctttgcaaca agctcttcct ttggcggaag cgctgcaatg gccttgagag ttttcaggtc 5281 acagactttg ccctcaacca cccctacttt aagttcaaga ttttcatact tttcagcata 5341 ctcaaccatg atttttgcag gggctacaac atctttatcg ctgaatgcca ttgaagtcgg 5401 accgttaaag taaggttcca actcttccaa gccattttct tttgctgcaa gttttgtcaa 5461 agtattttta actactttgt actccactcc ggcttttcta agtttgtttc taagttctgt 5521 gtcctgctca acggtaagac ctctgtaatc ggcaaatact atggttttag ccgatttcat 5581 attctcagac agctgcctga ctatctcttt tttctgctga aggactttct cacttggcac 5641 tgttatccac ctccttttaa aaaccgagtg tctatcttat gtctttctct tactagcgaa 5701 ctgaaataat aaaacccctc aatgcacata gacataaagg ggtaattgtc attatccgac 5761 tgctaaatca cattacctcg gcagggaagt ttgcaaacac ttttacactt tccgcttttg 5821 tgggaaagca acctgctgtc tatggcattt cctctattaa catttgtcaa tggatatatt 5881 ataacattat tctaaatttg taaagacatt ttttattcac ttactcttaa aggatttact 5941 tttatgccag gtcccattgt ggaagtaaca accacactct taagatactg gccttttgct 6001 gctgcaggtt tagccttgat tattgctgac atcaatgtgc ggaaattgtc aacaagcttt 6061 tcggtaccaa aggacacttt tcctataggg cagtgtataa tgttggtctt atccagcctg 6121 tactcaattt taccggcttt gatgtcggca attgccttgg ccacatccat tgttacggta 6181 ccggcttttg ggtttggcat taatcctttc ggaccaagca ctttaccgag tctacctaca 6241 acacccatca tatccggtgt tgcaactaca acgtcaaact caaaccagtt ttcattctga 6301 atctttgaaa ccaattcctc tgcaccaaca taatccgcac cggctttttc tgcttctgtg 6361 gctttttcac ctttcgcaaa aacaagaact cttactttct tacctgtacc gtgcggcaaa 6421 acaacggcgc ctcttacctg ctgatcggca tgtcttgagt caacaccgag tctgatatgt 6481 gcctccacag tttcatcaaa ctttgccttg gctgtctttt gcacaagttc cattgcctct 6541 acaggatcat acagctttgt tttatcaacc aattttacac tttcctgata tttcttgcct 6601 cttttcatcc aattcactct ccttacgtgg taatttttct cggaaactta cgtttcctcc 6661 caccttctac tttagaacaa catgaaaact ttgctttaaa ccttcaactt agtcctcaac 6721 aacaacgccc atgcttcttg cagtacctgc aatcatgctc atggcagcct caaggcttgc 6781 tgcattaagg tcgggcattt tcatttcagc tatttttctt acttcctctt tcgaaatctt 6841 ggcaactttt tccctgttcg gtctaccgga tcctttttct atcttgcatg ctcttttaat 6901 aagcactgac gccggcggag ttttcgtaac gaatgagaag gatctgtcag catatacggt 6961 aataaccaca ggtattacaa gacctgcgtc ctttgccgtc ctctcattga attccttaca 7021 aaacgccatt atgtttacgc catgttgtcc taaagctggt ccaaccggtg gagccggagt 7081 tgctttcccc gcaggaattt gaagttttat atagccgact actttctttg ccatattttc 7141 cacctcccaa ttctaatgat aataaatctc tctgccttga caaaaagaga tttattattt 7201 gcttttgcta atctgtagta aagattttat ttatagttaa aaatcatatt aggcatctta 7261 aaaaatacat cttcactata ttttctgaat ctggtgaagt tcaagttcca caggtgtctc 7321 tcttccgaac atcgaaacgg aaacacgcac ctttttcttt tccagattta tttcttcaac 7381 tattccgata aagttttcca aaggcccgga aaccaccctt acattgtctc cgacctcata 7441 gtccagcaca ggagcaaatt cttcaactcc catagccctt atctcttcat cggttaaagg 7501 cacggctttt gagcccggtc cgacaaaccc tgtaaccccc ctggtatttc tgaccacata 7561 ccaggattca tcattcataa tcatcttaac aagaacgtat ccggggaata ctttcttgag 7621 ggaggctttt ttcttgccgt cttttatttc aatctgctcc tccataggca caactatgtc 7681 aagtatataa tcctgcaagt tcctgttttc aacaattttc tcaaggtttg ccttcacttt 7741 gttttcatat ccggaatagg tatgaaccac ataccatttt gcttcctcgg atgcctcatt 7801 attcggcata aagctccaag tcctccttta taacttaata acccgcgctg ctttatgcat 7861 aagcttagta aataaggctg gacaatgcgc caaatcccag gtcaaacagc catatcaaca 7921 ctccgacaat aaagcacaaa atcaaaaccg tgatggtatt gtttataagc tgtgaccttg 7981 taggccaaat aaccctttta agctcgtttt taacgtcctt aaagtatttt gaaaatttcc 8041 ccgcggctct ctttaaaaaa ccttctttct ttttttcaac cttagagact ttcacttcct 8101 cagccatacg aacacatctc catttttaaa attttttgtt ctgtcagcac atcagaagtc 8161 ttcccccagc tgccgatatt attccctctt ttttattatt ttgtttcttt atgcatagta 8221 tgcttctggc agaacctgca atattttctc atttcgagtc tgtccgggtc gtttttcttg 8281 tttttcattg tattatagtt tctctgtttg cactctccac aagccaatgt aatttttact 8341 ctcacctttc acacctccaa ggtttgtcct aaaaatcaaa tatatttaaa ttatttaata 8401 tctcatttta actaacatct cattttgcca taaaaaaaaa gacgtacagc gaagcaatca 8461 ataatttaac atatttttta gcttaagtca agattttttt atatgtctgt ccataaattt 8521 tttgtcggtt cgttgtaaaa ttaaatgcaa cagtaaaatg aaataaaaaa ttgacaacca 8581 tagtgagaaa tcatgtatga tttaggtgtc caatccaaac atacaggagg ttctcaacta 8641 tggctgtaca atataagtct accacaactg agcataagtt taaacactta agtgtttatg 8701 aaagagggca gattgcagct cttttaaaag aaggaaagag tcaacgttat attgctaata 8761 aactaggtcg ctcgccaagt acaattagcc gtgaaattaa aagagggaca acaatgcaga 8821 tgagaactga tttatcgaca tacaaagtat attttcctga aacagggcag gcagtttatg 8881 agaaaaatcg tatgaattgc ggagcaaagc gtaaattggc tcaagttgaa gattttctta 8941 agtttgcaga agataagata ctacgcgaaa aatggtctcc agatgcagtt gttggtttat 9001 gtaggagaga ccccaagtgg caaaattcta ctattgtatg taccaaaaca ctgtataatt 9061 atatagacct gggactcata aaagtacgaa atatagattt aaatcttaaa ctacgtttaa 9121 aatctaaaat aaaaaggata cgtcaaaaca aacgggttgt agggaaaagc attgatcaaa 9181 ggccggaaga agtacaatca cgtcaaacct ttgggcattg ggaaattgat acggtaacag 9241 gcaaaaagtc taacgattca gtaattttaa ccttaactga acgaaaaacc cgctacgagt 9301 tattgtttct tttggacgca aaagacagta atactgttaa cgaggcactt tcagaactta 9361 agaattgtta tggtaaggat gtttcaaatg tatttcgcac tataacggca gacaatggtt 9421 ctgaatttag tagactatcc gaaatgttac aagggctagg aattgaagct tatttcactc 9481 atccttattc ctcatgggag agaggaacta atgaacgtca taatggactt attaggcgtt 9541 ttattcctaa aggaaaggct ataaaagatt tttctgaaga aacgataaaa cggatacaac 9601 aatggttaaa cagccttcca cgaaggatat taggttacaa aacacctgaa gaatgtttta 9661 atgaagagat acataacctg gtaaacaaaa atatatcagc aatagcctga gcccttcatc 9721 caagatattt taaagctcat ttgagggtag tcaagggtaa agactttgtc ttatctatcg 9781 ataacccttg actaccctct gcactcgctc aagatagtag ttaagaaggg ctaaaagatg 9841 atgttgtact ttagcaccta aaattatgga tgatttccac tataggtgtt gcatttaata 9901 ttgcaattta cataaatttt ttgtccaaat ctatttgtat cttaaacaag ccattttcta 9961 caaatccctt ttcgtctaaa aatatctttt tacaccgcac gtctttttta tgtgatcatt 10021 actcctcaat aagctcgctt aaaggagctc ccggcggaac aataggaaat accatttcat 10081 ccttgtcaat ttcaaaattg attaccacag gggtatcctc agacgccagt gctctgttta 10141 aagcttcatc gacttcttct ttggctgtga catttatacc gattgcaccg taagcttccg 10201 ccaaagcttt aaagtctgta catctgtcca gcgaggttga ggaatacctt ccgccgtaga 10261 acaactgctg ccactgtctt accattccca gagcatgatt gttgaatatc gcaactatta 10321 tcggaagctt gtactcaaca gctgtggcca gctcattgca gttcattctg aagctgccgt 10381 caccggcaac attaattacc tttttgtcgg gtcttccaat ccgggcgcca atgcatgctc 10441 caagaccata tcccatggta cccagaccac ctgaggacag gaactgtctt ggagaagtat 10501 atttgtaaaa ctgggcggcc cacatctggt gctgacccac ctcggtagtt attattgcct 10561 ctcctttggt aagttcatac attctctcaa taatatattg cggatgaagc ttgtcatcct 10621 gaggatactt aagaggatac agttccttcc actcattgat ttttctaacc cagtcagtgc 10681 attctttctt ctttactctc tcgttcagaa gctgcaatat tttcttgatg tttccctcaa 10741 gagcatattg aacattaata ttttttccga tttcggcagg gtcaatgtct atatgcatta 10801 tgcttgcttt aggtgcaaat ctctgaacat tgcttatcac tctgtcacta aatcttgcac 10861 caatcgcaat aaaaagatcc gattccgaaa ccgccatgtt tgtcgtcttt gtgccgtgca 10921 ttccaatcat tcccatgaac agctcatggg ttcccgggaa agcacccatg cccattaaag 10981 ttgtggtaac aggtatctgg gcatttgttg caaactcaaa aagttcttta tttgctcccg 11041 cgattgatac gcctcctccg gcgagaataa ccggtctttc cgctttgtta attgcctccg 11101 ccgcttcatc aatacacttt tcatccactc ttacaggtac ctcgggcaat tcctgagggg 11161 actttggttc atattcagca tatgccgccg ttacatcttt gcatatatca atcagaaccg 11221 ggcctggtct tccttctttt gctatgtaaa aggctcttcg cactatgtca gccagcttgt 11281 taacatcctt tactataaaa ttgtgttttg ttatgggcat tgttattccg gttatgtcaa 11341 cttcctggaa ggaatccttg cccaagagcg gcgtcgccac ctgtcctgta attgcaacca 11401 tcggaaccga atccatgtaa gcggttgcta ttcctgtaac cagatttgtg gccccggggc 11461 cggatgtggc aagacacacc ccaacctttc ccgttgccct tgcatagccg tctgcggcat 11521 gagcagctcc ctgttcgtgg gaagtcagta tatgccggat ttcattctgc gacctgtaca 11581 gttcatcata tatattcaat accgctccgc ccggaaatcc aaatattgta tccacacctt 11641 gttccttaag gcactcaatt aatattttcg aaccggtaag tttcattttt attaccccca 11701 taattttaca caaatgtctg ttaatcgaaa atcttaatcc acagttttat tacattattt 11761 gttttccagg atggcgcctt tgtttgcaga agaaacaagt tttgcatatc ttccaaggta 11821 accctttgtt attttcggcg ccggtgcctt ccactcttta agtctcttct gaatttcgct 11881 gtcggggatt tctacatcaa gctttccgtt aggtatgtct atgctgataa tatccccgtc 11941 tctgacaatt gctataggtc cgcccgccat agcctccgga gacacatgac ctattgaagc 12001 tcctctcgta gcaccggaaa aacgtccgtc agtaatcaag gcaacgtcct tgtcaagtcc 12061 catacctgca attgcggaag tcggggacag catttccctc atgccgggac ctcctttggg 12121 accttcatag cgtattatga ccacgtcacc tttgtttatt ttaccgttgt aaattgcttc 12181 tatggcagca tcctccgagt caaacaccct tgccggtccc ttgtgaacca acatttcagg 12241 ggcaacagcc gagcgcttta caaccgcacc gtccggagca agattacccc tcagcaccgc 12301 tataccgccc gtcggactgt aaggattgtc aatgcttctt ataacctcat agtccctgac 12361 ttttgcgttt tcaatgtttt cccttacagt tttgccggta acggttatca aatccagatg 12421 cagcaaattc ttctttgaaa gttccttcat cacagcaggt attcctcccg ccgcatagag 12481 gtcctgaaca tgatggtggc ccgccggagc cagcttgcaa aggttcggta ccttgctgct 12541 tatttcgttt attatgtcaa ggtttatctc tattcccact tcattggcaa tagcaggaag 12601 atgaagcaca gagtttgtcg agcagcccaa agccatgtcc acggccagag cattctcgaa 12661 agcctttgga gtgagaatat ccgaaggttt tatatctttt tcaaccaatt ccactatctt 12721 cattccggct ttctttgcaa ggcgtattct ttccgcataa accgcaggga ccgttccgtt 12781 gcccggaaga cccattccca aaacctcagt gaggcagttc atggagttgg cggtaaacat 12841 accggaacag gaaccgcagc cgggacaagc tttttcctca tactcccaaa cttctttctc 12901 cgtcatcttt cccgccttgt atgcccctac agcttcaaac acgctgttta aatccaggtt 12961 tttgtcatta tgccttaaag acagcatggg acctccgctt accacaatgg cgggaacatt 13021 tattcttgca gctgccatca gcattcccgg cactatcttg tcacaattgg gtatgaaaac 13081 cattccgtca aagctgtggg ccaacgccat tgcctcgcag gagtcggcta taagctccct 13141 tgtggccagg gaatatttca ttcccgtatg acccatcgct atcccgtcac acacaccgat 13201 tgcaccgaac tcaacaggag tacctcctgc cattctgata cctgccttta cagcttcggc 13261 aatcttgtcc agatgtatat gtcccggaac aatttcgttt ctggaattaa caactcctat 13321 aagcggtctt tccaattctt catctgtata gcccattgct ttaaacaaag ccctgtgagg 13381 ggctctttct atgccttttt ttaccgcatc gcttctcata atgcattcct ctctttccaa 13441 aatatatatt aactcaatat taaactcaaa aacaaaattc agacatttac tatgatttcc 13501 aatttattat aataatagcc gggtttgccc ggctattgtc ttgtttatta tttattttac 13561 tactacaaag ctttgattgt ccagtgtttt agaaaaaaag catttttcgt taaatatttc 13621 tatggactta aaataacatt cattaattgt gcccagaaat tgagccaaat catattcacg 13681 ccatccatgt tcacactatg acgtctttcg ggaacatcca gtccactata aaattgttgt 13741 ggtacagtac ttttgcgaca gtggaacttt caattgcctc aaacactccc tgaaatttcg 13801 gtgacgcgct aaacataatc agtatcgcaa aaactatata aaccgttaag agtccttcca 13861 gtgcgcccaa aataaatcct cccgctttgt ccgcctgttt tacgatcgga agagaagtga 13921 cacctttcag cacacgctca agaacaaaca atcccactct taccgccaca aacataacta 13981 tgagacttat aatatcgact atcatatggg caagaacgtc gcttaaggaa tccataatgg 14041 ttgaaacatc aatcagctcc gtaaggctgg gcaaactctt gagcaagctg ttttttatca 14101 tgcccttcat aaatccgggc aggctcaagc cgtcaacaac cgattgggct gtagcctttg 14161 ccgctgcatc aaacggaaga cttaaagcct catgccgaag catgagatta tggtaaattc 14221 ccttctttat gctgtggaac acctgagaat ttgataaaat ctttgcaatt atcggataaa 14281 gctttgccga taaaactgct gatatgaaaa aagaagccag cttaaacact gaataaacaa 14341 tgcctttctt aagcccaatc catgccagta ccaaaataat cgcaatgact accaaatcaa 14401 tccagttcat attttccccc ttacatgtta attcattttt aaaaggataa cgccttcttt 14461 tgtaattgcc aaagcctcat ttgtgccaag aaacttcact tcatttatca aataatccga 14521 agcatagctt cccctgagtc tcccggcaac atctataaaa taaacacact tggatgtgtt 14581 tatggctata aaatcatcat ttgcagctat gttcacaaca tcctggtcaa ttctgtaatc 14641 tgcgacttcc ctgcctttgg aatcaactat aagaacatgt cccgcttctc ccatcattcc 14701 tttttcgctg aatgcaaaca cggcgtatct gcctttcata acgtccatgc tgtatacttt 14761 gccgcttatt gtcctgctcc acttttcttc aaaattttgt ccaaacagat aaatcgctga 14821 gtcggacact gccacaatgg tatcgtcatt caaatagcgg attgaaggaa aaataacatt 14881 ctcgaaagtt ttgccagcta cattcccacc tgaaatcgta tacaaatgaa gtcctgtatt 14941 tatacttaca cccgaaatat caacacagtt tatcagaacc tccctgccgg agggagacac 15001 ctccgacgag atggcgtaag tttctcccaa tattttggta aaaagaaaaa ctccctgttt 15061 gttgtacact gatacagcag acttatccct ctctgctttg tgaaggacgg taacataacc 15121 cgcgctgttt atatttgcat ttattatact gtaatccagc tttttttccc actttttctc 15181 cttgccggca aatacataaa tgtcttttga ttcgagccct gcaaccaaaa gatactcgcc 15241 cgcggactta aaatacggca aattcaaaga aacagtatat atccactcct cttcaccatt 15301 tctgttgaga tattccaaac catttttcgt gcatcttaca atatagccct tgtaagcacc 15361 aaactcagag ttcaaactgt cctcatatct taacaaggtg ccggttactt catacacatc 15421 tttatctccg acgaaaaacc cattggctat tatatccctt atgcttacac ttttaatgtc 15481 tatacccttg ctgttgagat aggcaacagc agccgtacac acaactatga tcacaagtac 15541 gaaaaataga agtatgctca ttctgctcga aggttgccgc atctttggta tgttttcagg 15601 gctgttcacc ttggcctcac tccatatttt tcttaagtaa taagacaagt ataaaaatat 15661 tgtatcatat atattgtttc tacttcaatc tatcggtccc tgtttttgta ctccctcttg 15721 ctggaaacaa aagcaaaact tgccgcaagc tttgaaacaa gcatgtttag tccctggatc 15781 agtatcataa agagcataaa cattcctaca tacccaaatg ccggatatac ggatgatata 15841 agtccggaaa atcccacatt ggaaattggc acaacaaagc cgcatattac cgggatcagt 15901 atctttctgc ttattctcac tttatttgct attctctcga taaaaccata ccccgatgtt 15961 acagccgaaa tgaacattgc aagaagaagc agtattttat agaattctcc caaaataaaa 16021 ttatgcctgc cgattatact taaaaccgga atctcttcag agacaatatc gggataaaat 16081 acataaagta taatgttcaa aactattgct ataaaactta gcgcaagccc gccgataatt 16141 cctcccgcag cagcaatttt cctgcttttt aagtaaggca gaaggctgca cattaccgca 16201 acgccgctta tgctgttgta gcttacatag agaagagaag agacgagcca gttgtcggtt 16261 atcgcatcaa atcccggtac agggctgaat acccatgcat ccatattgac tataacatag 16321 aatcctgctc caagaatacc cagcaccaaa atgggagtca caaaagaact taatgccaca 16381 atccctttga tgtcagtaag aaagacaatc atgcataaaa ttgcagctat cagcgtgcca 16441 tagtgataag gtatgtttaa cgcaccggat attatgcttc ctgctccggc aaccattatg 16501 cacaatacag aacccataaa caacgttacc ataatctcca taatccagcc aattacccat 16561 ccgacgctgg gaaaaaggaa ttcctcgtag tttcttattc tttcgctgta gacttttact 16621 aacacaatat atcctatcaa cgcaaacaaa actcctgcca gaacaatgcc gtaaaatccc 16681 ccctcgtaat acgacgaaaa aaactgcata atttcctgtc ccgaagcaaa tccagcccca 16741 atcaccgttg ccatataaat gcttgcaact ttcaatatat tttttacatc ctgagacaaa 16801 aaaacaactc cttaccatag aatcattctc tagaataatt ctatgtagga gttgtttttc 16861 aaattaccag ttaattttat tcttctttgc cttcatcctc gctgttaagg aacttcataa 16921 gctcttcata agtctttctc gggccgtcct cggcatcaaa ggcatccttt cttacaacaa 16981 tacctgtata cttgccgttt ttcaccgcat aataggttct ttcatcccga ggaatgaatt 17041 ccaccgttac tttgcccggt tcggtattga ggtggtaaac tatactgatt tcggcttctc 17101 cggaaggtgt cgcatccggt tcgatttcac tggctgtaat gcctataatt gtggaataaa 17161 aattcctcaa ttttgatatg ccttcttctc cctttttcgc ctccacatca attccgtcaa 17221 tataatgctt gtatttcttc tcctcctcgt tttccccttc ttcggattcg ccggacgtat 17281 ctgattcatc agatttgtcg gtttcctgag tctgtgtctc gtcttttact tcttcaatct 17341 taatttttat cgttcttccg tctatgttga aatctaaatc cgttacagta tagatatatg 17401 gtgcatatat gaaaccgtcc atcatttcca gggccggagt atcaagaaaa cctaaagtgc 17461 cgggatttat tgtaaacact tcattggttc cttcaaacat tccgtaagac tcagcattgt 17521 tttctttaac atcgccgata agcaaagtca ctcttttact tccggcagcc gcttcaacaa 17581 cataggacgg attgtcaagc ccgtattttg aaaggtcttt aacatccttc tcaatataat 17641 ctataacttc cgccctcaca agcgcctcca gggccgtatt cagcctgacc atgttggcgt 17701 ttgcctctat gggctctgtc atatgccatc ccttgtcatc cgactttaca agtttaaagg 17761 accgttttcc cttctttgtt acagccagct ccgttacatc ctgtgatgtg acatcaagaa 17821 caaacctgtt ccggatgctg ttttcgtttg ccttgagtat gtttcctgcg taataggcaa 17881 ttgtatatac tttgtttttc cctttttctc ttatatagta tccgcttcct gtaggtgttt 17941 gatcacccac ttctatttcc gcaaccgtac cgtccgaaaa tttcagcgtt accgttacag 18001 gattgtcaag accatactgt ccaagattct tcggatcgct ttccacctct ttggaagcat 18061 aaagatcgca gatattggaa actatacttt caacctggac cttgtttatc tcaaagtccc 18121 ctccggaaac caactgccat tcggaatcat tctttttcag gacaaaagtc tttcccccgc 18181 ttactatcgt aagctcttcc gccttttcct tgtccagctt gtatacttca atctgtttgt 18241 aactatcctc ttcttcagcc ggaagcctgt ttttaatagc aaagtacgca cctatcagac 18301 ccaggaaaag caccacaaga ataactatat tcctgtatag tttcaaagac ctcatagatg 18361 cctcctcctc agccatataa taatac //