LOCUS NZ_ACVX01000038 31690 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00026, whole genome shotgun sequence. ACCESSION NZ_ACVX01000038 NZ_ACVX00000000 VERSION NZ_ACVX01000038.1 GI:256005716 DBLINK Project: 55637 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. REFERENCE 1 (bases 1 to 31690) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Hemme,C.L. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The draft genome of Clostridium thermocellum DSM 2360 JOURNAL Unpublished REFERENCE 2 (bases 1 to 31690) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Hemme,C.L. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (17-JUL-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000038. URL -- http://www.jgi.doe.gov JGI Project ID: 4085028 Source DNA and Bacteria available from Christopher L. Hemme (hemmecl@ou.edu) Contacts: Christopher L. Hemme (hemmecl@ou.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Annotation by JGI-ORNL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Clostridium thermocellum DSM 2360 Culture Collection ID :: DSM 2360 GOLD Stamp ID :: Gi02594 Isolation Site :: contaminant of Clostridium thermocellum strain LQ8 Isolation Country :: USA Oxygen Requirement :: Anaerobe Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Sporulating Temperature Range :: Thermophile Temperature Optimum :: 60 C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Phenotypes :: Cellulose degrader, Ethanogenic, Ethanol production Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Location/Qualifiers source 1..31690 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene 173..2746 /locus_tag="ClothDRAFT_2533" CDS 173..2746 /locus_tag="ClothDRAFT_2533" /inference="protein motif:TFAM:TIGR01451" /note="TIGRFAM: conserved repeat domain protein; PFAM: S-layer domain protein; KEGG: S-layer homology domain containing protein" /codon_start=1 /transl_table=11 /product="conserved repeat domain protein" /protein_id="ZP_05430672.1" /db_xref="GI:256005717" /db_xref="InterPro:IPR001119" /db_xref="InterPro:IPR001434" /translation="MKKIWGRKVLGFAVGVCLLLTMICQNVAFVSAEPEEKTVVVNFE ESLNETMSKTIEILNLFDISEIVVDSGKVSYSREGDKVTVTVSEGVYRVGPHTQNVTL TIEDDKGIFDEKTSYNVDGYEGILTKIDSGYDEAKGLYWVKYQGSVTKENCKFYQYTV TIKYTENVRPEVYLTEPKRGMITNGKINVQGYVRDENIGDELKLFFSFDSYDESMTGN LLNENSIISDGTWQEISGTIDLSPFNLKDGDHDFYFWAVDKRGVRSVGEIIRFTLDTV PPEAPVLTPDKTESTNQSVVVSVYYPPDAVGREIKINDGPWMPITDITKNDQIIMDEN GKIEARAIDEAGNISEVAELEIKNIDKIPPTAPTINTSADETTEQPIKATIVPGVDNE SGVDRTEYCLRGASTKDWEKYDEGTEITITALGETEICARTIDNAGNISAETVKKVTI KKKEDSGGNNGGSGGTGGNSGNNGGSGGTGGSGSSGGSGSNSGGGNNDGNGNDGKKDD EILQPEPNIPGAGGSPVDLSVFISADKSKYEEGEVITFNITYKNKTNVQANNVIVKAG IPANTTVEDIAGGTQNGNDIEWKIESLKANSSGKIQYKVKVNLLEVPEISSSATASIT ASGTLINKDDDESRTIFLLYSNRFGENFHGKYITGYEDNTFRPLNNITRAEVATIMAN ILGLKQEVAGGKTYTDLSKSHWAYNNIIAVTEKGLFTGYEDGSFRPDNFITRAEFATV LANYLGLKNVEHDELNFADIENHWAKNFIEEIYRVRLIEGYLENGLRLFKPDNYITRS EAVTIINKMLFRGPLEGAKVPFTDVEEGYWAYGHILESSIDHYYVRNKDQSETIVNKK Q" sig_peptide 173..271 /locus_tag="ClothDRAFT_2533" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.994) with cleavage site probability 0.482 at residue 33" misc_feature <2135..2203 /locus_tag="ClothDRAFT_2533" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" misc_feature 2255..2386 /locus_tag="ClothDRAFT_2533" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" misc_feature 2438..2575 /locus_tag="ClothDRAFT_2533" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" gene complement(2926..4527) /locus_tag="ClothDRAFT_2534" CDS complement(2926..4527) /locus_tag="ClothDRAFT_2534" /inference="protein motif:TFAM:TIGR00503" /note="TIGRFAM: peptide chain release factor 3; small GTP-binding protein; PFAM: protein synthesis factor GTP-binding; elongation factor Tu domain 2 protein; KEGG: mxa:MXAN_5992 peptide chain release factor 3" /codon_start=1 /transl_table=11 /product="peptide chain release factor 3" /protein_id="ZP_05430673.1" /db_xref="GI:256005718" /db_xref="InterPro:IPR000795" /db_xref="InterPro:IPR004161" /db_xref="InterPro:IPR004548" /db_xref="InterPro:IPR005225" /translation="MQNKISYEVERRRTFAIISHPDAGKTTLTEKLLLYGGAIRLAGS VKARKANKYATSDWMEIEKQRGISVTSSVLQFEYNNYCINILDTPGHQDFSEDTYRTL MAADSAVMLIDGAKGVEEQTIKLFHVCKMRGIPIFTFVNKMDRASKDPFELMEELENV LGIRSYPMNWPIGTDGDFKGVYNRKLSQIELFEGGNHGQTVVSSIKGSVDDKIFADLL GDHYHKKLCEDIELLDMAGDPFDKEKILKGELTPMFFGSAMTNFGVQPFLEEFLQLAP KPGIKMSSEGEVDPESDKFTGFVFKIQANMNPAHRDRIAFIRICSGRFTRGMTVYHVQ QGKEVRLSQPQQFMAQERTIVEEAYAGDIIGVFDPGIFHIGDTLSEGNSNLRFEGIPI FPAEHFAKVTPVDTMKRKQFIKGINQLSEEGTIQVFKQIDIGFESIIVGVVGILQLEV LEYRLKHEYGVDLRIQKLPHRHARWIVSSDTEPRNLTLTSTTMIVHDQDERYVLLFEN EWSIRWAEEKNPSLKLEDIAKRDLL" misc_feature complement(2944..4506) /locus_tag="ClothDRAFT_2534" /note="peptide chain release factor 3; Provisional; Region: prfC; PRK00741" /db_xref="CDD:179105" misc_feature complement(3694..4497) /locus_tag="ClothDRAFT_2534" /note="RF3 subfamily. Peptide chain release factor 3 (RF3) is a protein involved in the termination step of translation in bacteria. Termination occurs when class I release factors (RF1 or RF2) recognize the stop codon at the A-site of the ribosome and...; Region: RF3; cd04169" /db_xref="CDD:133369" misc_feature complement(4450..4473) /locus_tag="ClothDRAFT_2534" /note="G1 box; other site" /db_xref="CDD:133369" misc_feature complement(order(4060..4062,4072..4074,4180..4185, 4252..4257,4309..4314,4426..4431,4438..4440,4447..4452, 4462..4464,4468..4470)) /locus_tag="ClothDRAFT_2534" /note="putative GEF interaction site; other site" /db_xref="CDD:133369" misc_feature complement(order(3751..3759,4096..4098,4102..4107, 4447..4464)) /locus_tag="ClothDRAFT_2534" /note="GTP/Mg2+ binding site; other site" /db_xref="CDD:133369" misc_feature complement(4312..4347) /locus_tag="ClothDRAFT_2534" /note="Switch I region; other site" /db_xref="CDD:133369" misc_feature complement(4324..4326) /locus_tag="ClothDRAFT_2534" /note="G2 box; other site" /db_xref="CDD:133369" misc_feature complement(4258..4269) /locus_tag="ClothDRAFT_2534" /note="G3 box; other site" /db_xref="CDD:133369" misc_feature complement(4207..4263) /locus_tag="ClothDRAFT_2534" /note="Switch II region; other site" /db_xref="CDD:133369" misc_feature complement(4096..4107) /locus_tag="ClothDRAFT_2534" /note="G4 box; other site" /db_xref="CDD:133369" misc_feature complement(3751..3759) /locus_tag="ClothDRAFT_2534" /note="G5 box; other site" /db_xref="CDD:133369" misc_feature complement(3385..3639) /locus_tag="ClothDRAFT_2534" /note="RF3_II: this subfamily represents the domain II of bacterial Release Factor 3 (RF3). Termination of protein synthesis by the ribosome requires two release factor (RF) classes. The class II RF3 is a GTPase that removes class I RFs (RF1 or RF2) from the...; Region: RF3_II; cd03689" /db_xref="CDD:58080" gene 5089..5514 /locus_tag="ClothDRAFT_2535" CDS 5089..5514 /locus_tag="ClothDRAFT_2535" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: lamin_L2; nuclear lamin" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430674.1" /db_xref="GI:256005719" /translation="MALTKERLLEEISESSGFSTVFNSCSRELQNLLINLVIEISRYS CNREGYVKNMKETSIRFEKPYLVGRKNQNYCMLTLRPRLNQIVVDVRTDGKFINSETL KLINLGNKYNGGFEWHRFVVKDENEIKEAVRLISKCYEG" gene 5739..6575 /locus_tag="ClothDRAFT_2536" CDS 5739..6575 /locus_tag="ClothDRAFT_2536" /inference="protein motif:PFAM:PF08902" /note="PFAM: Domain of unknown function DUF1848; KEGG: dal:Dalk_4282 domain of unknown function DUF1848" /codon_start=1 /transl_table=11 /product="protein of unknown function DUF1848" /protein_id="ZP_05430675.1" /db_xref="GI:256005720" /db_xref="InterPro:IPR014998" /translation="MLEIISCSRRTDIPAFYYDWLQECLKNKYAVVKNPYNKSTYMVD LSCEKVHSICLWSKSFANVLKDPKYLSLYNLYFQFTITGYSKFLEPNVIDTNEAVRQM EQLAQKYSPRQINWRFDPIIFSAEGEKEPTPDNFERARLKVFENLCKDISSFGVNRCT ISFLCLYKKVEQRMKKCNFTYFPPSQQKQIEFVSRLVEIADKYQVTIYTCSSPVIESV PGVKKGHCIDGAYLEELFGKRASRAKDSGQRKECGCTRSKDIGGYDNQSCRHGCVYCY AR" misc_feature 5748..6569 /locus_tag="ClothDRAFT_2536" /note="Domain of unknown function (DUF1848); Region: DUF1848; pfam08902" /db_xref="CDD:149840" gene 6804..7187 /locus_tag="ClothDRAFT_2537" CDS 6804..7187 /locus_tag="ClothDRAFT_2537" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: mxa:MXAN_4369 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430676.1" /db_xref="GI:256005721" /translation="MFEKAKNDDWHVLLTPTKPVPKDWFCEIKNAEILGLACGGGQQM PIFAALGAKCTVLDYSEMKDEQLYRESIKNNWGIQFSHTIEEQIGGQLRAGFILTDIY QDTNGFGRLHEFNVPTYYATRAIKI" gene 7276..7713 /locus_tag="ClothDRAFT_2538" CDS 7276..7713 /locus_tag="ClothDRAFT_2538" /inference="similar to AA sequence:KEGG:RL1234" /note="KEGG: rle:RL1234 putative glyxoylase" /codon_start=1 /transl_table=11 /product="putative glyxoylase" /protein_id="ZP_05430677.1" /db_xref="GI:256005722" /translation="MVGVEIDMIVADSLKALELYEKIFDIQRVEVSNLPKGENEVVFT LYGVRFHMLDENPEFGLKAPDRDRPNTIWFNVSVPDIKETFSKAINAGCTEIQPATEM PDYGLSNAIFADPFGYQWMLHQIQREVSFEERIRLWEDSRKEK" misc_feature 7300..7647 /locus_tag="ClothDRAFT_2538" /note="This conserved domain belongs to a superfamily including the bleomycin resistance protein, glyoxalase I, and type I ring-cleaving dioxygenases; Region: Glo_EDI_BRP_like_8; cd07246" /db_xref="CDD:176670" misc_feature order(7300..7302,7345..7347,7387..7389,7408..7413, 7417..7425,7480..7488,7492..7509,7525..7527,7588..7590, 7606..7608,7630..7641) /locus_tag="ClothDRAFT_2538" /note="putative dimer interface; other site" /db_xref="CDD:176670" gene 8199..9872 /locus_tag="ClothDRAFT_2539" CDS 8199..9872 /locus_tag="ClothDRAFT_2539" /inference="protein motif:PFAM:PF02824" /note="PFAM: TGS domain protein; SMART: AAA ATPase; KEGG: Phosphoribulokinase / Uridine kinase family protein" /codon_start=1 /transl_table=11 /product="TGS domain protein" /protein_id="ZP_05430678.1" /db_xref="GI:256005723" /db_xref="InterPro:IPR000764" /db_xref="InterPro:IPR003593" /db_xref="InterPro:IPR004095" /translation="MNENNLNMIKVVFPDNSEREVYEGISLQELSESCKNQYKSTIVA AKVNNDIKELSYRLNESCRVEFIDLTDDDGMRIYKRSLSFILIKAVNDLFPDRKVIIC HSISKGIYCEVKGDTPLTVEEVDMIKNRMKEIVNLKIPFIKKIMSLDEAREVFRKIGR MDRFRSIEYRKKPYVTLYECDGFQDYFYGYMVPHTGYLDKFDLKYYQPGLILMSPEKT SPDAIPQFKEQKKLFSIFAEYKKWGKILGVEDVSALNDIVKEGKINELIRVAEALHEK KIAQIADMIAFNEHKKKVVLIAGPSSSGKTTFAHRLSIQLKVNGLRPVTISLDDYFVD RELTPKDENGEYDFEALEAIDIKLFNRHLAELIEGKEVDVPIFNFPKGCRESFCRKLK IDEDQIIIIEGIHGLNEKLTASIPKENKFKIYVSALTSMNIDEHNRIPTTDTRIIRRI VRDYQFRGCSAANTIKRWPSVRRGEERNIFPFQEEADVMFNSALMFELGVLKTYAEPL LMEIDSSEPEYSEARRLIEFLNNFLPIDSKEIPANSIIREFIGGSCFYQ" misc_feature 8220..>8807 /locus_tag="ClothDRAFT_2539" /note="threonyl-tRNA synthetase; Reviewed; Region: thrS; PRK00413" /db_xref="CDD:179011" misc_feature 8223..8405 /locus_tag="ClothDRAFT_2539" /note="TGS _ThrRS_N: ThrRS (threonyl-tRNA Synthetase) is a class II tRNA synthetase that couples threonine to its cognate tRNA. In addition to its catalytic and anticodon-binding domains, ThrRS has an N-terminal TGS domain, named after the ThrRS, GTPase...; Region: TGS_ThrRS_N; cd01667" /db_xref="CDD:133437" misc_feature 9066..9761 /locus_tag="ClothDRAFT_2539" /note="Uridine kinase [Nucleotide transport and metabolism]; Region: Udk; COG0572" /db_xref="CDD:30917" misc_feature 9078..9674 /locus_tag="ClothDRAFT_2539" /note="Uridine monophosphate kinase_like (UMPK_like) is a family of proteins highly similar to the uridine monophosphate kinase (UMPK, EC 2.7.1.48), also known as uridine kinase or uridine-cytidine kinase (UCK); Region: UMPK_like; cd02028" /db_xref="CDD:30201" misc_feature order(9093..9095,9108..9119) /locus_tag="ClothDRAFT_2539" /note="active site" /db_xref="CDD:30201" gene 10005..10358 /locus_tag="ClothDRAFT_2540" CDS 10005..10358 /locus_tag="ClothDRAFT_2540" /inference="similar to AA sequence:KEGG:HRM2_16570" /note="KEGG: dat:HRM2_16570 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430679.1" /db_xref="GI:256005724" /translation="MKAREILEILNAELLTGEENLDMEFNAACGSDLMSDVMAYVKEN VVLLTGLVNPQVIRTAEMMDIKLVVFIRGKRPEEKIIQMAKEKGIAIMTTNDPMFIAC GKLYSAGITERGVWD" misc_feature 10017..10328 /locus_tag="ClothDRAFT_2540" /note="DRTGG domain; Region: DRTGG; cl12147" /db_xref="CDD:159807" gene 10355..10804 /locus_tag="ClothDRAFT_2541" CDS 10355..10804 /locus_tag="ClothDRAFT_2541" /inference="protein motif:PFAM:PF02518" /note="PFAM: ATP-binding region ATPase domain protein; SMART: ATP-binding region ATPase domain protein; KEGG: dat:HRM2_16560 RsbW2" /codon_start=1 /transl_table=11 /product="putative anti-sigma regulatory factor, serine/threonine protein kinase" /protein_id="ZP_05430680.1" /db_xref="GI:256005725" /db_xref="InterPro:IPR003594" /translation="MSDGIIRKNFVIPANDFTLAGEASSTVKKMLIQLGAEPQKIKKT AISMYEAELNAVIHANGGVAEVEIDRNKVFIRIKDEGPGIPDLELAMQEGYTTAPDSI REMGFGAGMGLPNMKRYADKLEIITEVGKGTIVEITVFLTDNEKENH" misc_feature 10514..10771 /locus_tag="ClothDRAFT_2541" /note="Histidine kinase-like ATPases; This family includes several ATP-binding proteins for example: histidine kinase, DNA gyrase B, topoisomerases, heat shock protein HSP90, phytochrome-like ATPases and DNA mismatch repair proteins; Region: HATPase_c; cd00075" /db_xref="CDD:28956" misc_feature order(10514..10516,10523..10525,10583..10585,10589..10591, 10595..10597,10601..10606,10682..10693,10727..10729, 10733..10735,10748..10753,10757..10759) /locus_tag="ClothDRAFT_2541" /note="ATP binding site; other site" /db_xref="CDD:28956" misc_feature 10514..10516 /locus_tag="ClothDRAFT_2541" /note="Mg2+ binding site; other site" /db_xref="CDD:28956" misc_feature order(10595..10597,10601..10603,10682..10684,10688..10690) /locus_tag="ClothDRAFT_2541" /note="G-X-G motif; other site" /db_xref="CDD:28956" gene 10823..12169 /locus_tag="ClothDRAFT_2542" CDS 10823..12169 /locus_tag="ClothDRAFT_2542" /inference="protein motif:PFAM:PF04060" /note="PFAM: Fe-S cluster domain protein; 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: dat:HRM2_16550 iron-sulfur binding hydrogenase" /codon_start=1 /transl_table=11 /product="Fe-S cluster domain protein" /protein_id="ZP_05430681.1" /db_xref="GI:256005726" /db_xref="InterPro:IPR001450" /db_xref="InterPro:IPR007202" /db_xref="InterPro:IPR017900" /translation="MSPYFHSVTLDEVKCKGCTNCIKRCPTEAIRVRKSKARIINERC IDCGECIRVCPYHAKKAITDPIGLINDYKYKVAIPAPSLYGQLKSAPSRDHILTALKM CGFDDVFEVARAAEIITSETKKILAQQRFEKPLISSACPAVVRLIQVRFPNLIDNILK LKSPMEVAAKIAKDEISRNRKIRQEDIGVFFITPCAAKVTSIKAPLDKEKSFVDGAIS ISEIYLKILSALGKIDKPEKLSRAGFVGVRWANSGGESIALGTDKFLAVDGIHNVIAI LEEIEDEKLEDIDFVEALACQGGCLGGPLNVENMYVARKTIKKFIDDAKEKGLETAAD ENCNYDIAWTGKVEYKPVMKLDKDFDVAMKKLETLNVINSGLPGLDCGACGAPSCRAL AEDIVRGNANETDCIFKLREKVRDLAFQMMELEAKMPPVLDKDETSENKSKKRGEF" misc_feature <10847..>11005 /locus_tag="ClothDRAFT_2542" /note="Dissimilatory sulfite reductase (desulfoviridin), alpha and beta subunits [Energy production and conversion]; Region: DsrA; COG2221" /db_xref="CDD:32403" misc_feature 11027..11734 /locus_tag="ClothDRAFT_2542" /note="Iron only hydrogenase large subunit, C-terminal domain; Region: Fe_hyd_lg_C; pfam02906" /db_xref="CDD:145848" misc_feature 11936..>12154 /locus_tag="ClothDRAFT_2542" /note="Predicted NADH:ubiquinone oxidoreductase, subunit RnfB [Energy production and conversion]; Region: COG2878" /db_xref="CDD:32705" misc_feature 11948..12046 /locus_tag="ClothDRAFT_2542" /note="Putative Fe-S cluster; Region: FeS; pfam04060" /db_xref="CDD:112857" gene 12169..12498 /locus_tag="ClothDRAFT_2543" CDS 12169..12498 /locus_tag="ClothDRAFT_2543" /inference="protein motif:PFAM:PF07085" /note="PFAM: DRTGG domain protein; KEGG: dat:HRM2_16550 iron-sulfur binding hydrogenase" /codon_start=1 /transl_table=11 /product="DRTGG domain protein" /protein_id="ZP_05430682.1" /db_xref="GI:256005727" /db_xref="InterPro:IPR010766" /translation="MKVKEFAEQLKMEILTGEKGLEKEIKGMYICDLLSWVMSHAAKG DAWITVHTHVNIVAVALMADIPCIIIPEGIEVEEATIKKAVEEDIAILRTSKTAYQIA CEAGKLL" misc_feature 12181..12474 /locus_tag="ClothDRAFT_2543" /note="DRTGG domain; Region: DRTGG; cl12147" /db_xref="CDD:159807" gene 12495..13205 /locus_tag="ClothDRAFT_2544" CDS 12495..13205 /locus_tag="ClothDRAFT_2544" /inference="protein motif:PFAM:PF02811" /note="PFAM: PHP domain protein; SMART: phosphoesterase PHP domain protein; KEGG: dat:HRM2_16540 metal-dependent phosphoesterase (PHP family protein)" /codon_start=1 /transl_table=11 /product="PHP domain protein" /protein_id="ZP_05430683.1" /db_xref="GI:256005728" /db_xref="InterPro:IPR003141" /db_xref="InterPro:IPR004013" /translation="MIVYTDLHIHSALSPCSDNEMTPNNIVNMAFLKQLDIIAVTDHN SAENCVAVVECAKKRGIVAVPGMELETREEVHLVCLFPGIDEALDMQKIVYNALPDIK NREDIFGEQIVMDAGDNVLRHVDRLLLTASNLSVEDVFSHVAALGGVVIPAHVDRDSY SIISNLGIIPENPEIKYLEISKFCDKTKYLEKNPHLGRFNFIRSSDAHSLGDILEREN SIELEEISIECLIETLKK" misc_feature 12495..>13121 /locus_tag="ClothDRAFT_2544" /note="PHP family phosphoesterase with a Zn ribbon [General function prediction only]; Region: COG1379" /db_xref="CDD:31569" misc_feature 12510..12701 /locus_tag="ClothDRAFT_2544" /note="DNA polymerase alpha chain like domain; Region: POLIIIAc; smart00481" /db_xref="CDD:128757" gene 13467..13964 /locus_tag="ClothDRAFT_2545" CDS 13467..13964 /locus_tag="ClothDRAFT_2545" /inference="protein motif:TFAM:TIGR01958" /note="TIGRFAM: NADH-quinone oxidoreductase, E subunit; PFAM: NADH dehydrogenase (ubiquinone) 24 kDa subunit; KEGG: pca:Pcar_0833 NADH:ubiquinone oxidoreductase 24 kD subunit" /codon_start=1 /transl_table=11 /product="NADH-quinone oxidoreductase, E subunit" /protein_id="ZP_05430684.1" /db_xref="GI:256005729" /db_xref="InterPro:IPR002023" /translation="MSANGCCCCGGVDSREQKLNEIIEKYKNTKGALVPVLHEAQEVY GYLPLEVQKKIAEGLNIPLAEVYGVVTFYTQFSLNPKGKYKIQVCMGTACYVKGSGAI LEKLKEKLEIDVGECTSDGKFSLEACRCIGACGLAPVIMINDDVYGRLVPDDIEGIIE KYKKE" misc_feature 13518..13955 /locus_tag="ClothDRAFT_2545" /note="NADH-quinone oxidoreductase, E subunit; Region: nuoE_fam; TIGR01958" /db_xref="CDD:131013" misc_feature 13713..13952 /locus_tag="ClothDRAFT_2545" /note="TRX-like [2Fe-2S] Ferredoxin (Fd) family, NADH:ubiquinone oxidoreductase (Nuo) subunit E subfamily; Nuo, also called respiratory chain Complex 1, is the entry point for electrons into the respiratory chains of bacteria and the mitochondria of...; Region: TRX_Fd_NuoE; cd03064" /db_xref="CDD:48613" misc_feature order(13719..13721,13725..13727,13845..13853) /locus_tag="ClothDRAFT_2545" /note="putative dimer interface; other site" /db_xref="CDD:48613" misc_feature order(13731..13733,13746..13748,13854..13856,13866..13868) /locus_tag="ClothDRAFT_2545" /note="[2Fe-2S] cluster binding site; other site" /db_xref="CDD:48613" gene 13961..14527 /locus_tag="ClothDRAFT_2546" CDS 13961..14527 /locus_tag="ClothDRAFT_2546" /inference="protein motif:PFAM:PF02518" /note="PFAM: ATP-binding region ATPase domain protein; SMART: ATP-binding region ATPase domain protein; KEGG: dat:HRM2_16530 sensory signal transduction histidine kinase (ATPase domain protein)" /codon_start=1 /transl_table=11 /product="histidine kinase" /protein_id="ZP_05430685.1" /db_xref="GI:256005730" /db_xref="InterPro:IPR003594" /db_xref="InterPro:IPR004358" /db_xref="InterPro:IPR005467" /db_xref="InterPro:IPR013838" /translation="MRDLSLHLMDIVQNSIDAKASKISILICADKKSDLLEIEVKDNG IGMDSDFLKEVVNPFTTTRDTRRVGLGIPLLKASAKRASGDLEITSEKLVGTTVKASF KISHIDRLPLGNLAQTMESLIVARPDIEYDLTLDNKKERFIFNSFEIKRRIGEVPLTH FEVLKWIGEYVNDGVKTIFGGILDEILG" misc_feature 13982..14266 /locus_tag="ClothDRAFT_2546" /note="Histidine kinase-like ATPases; This family includes several ATP-binding proteins for example: histidine kinase, DNA gyrase B, topoisomerases, heat shock protein HSP90, phytochrome-like ATPases and DNA mismatch repair proteins; Region: HATPase_c; cd00075" /db_xref="CDD:28956" misc_feature order(13988..13990,14000..14002,14009..14011,14078..14080, 14084..14086,14090..14092,14096..14101,14165..14176, 14222..14224,14228..14230,14243..14248,14252..14254) /locus_tag="ClothDRAFT_2546" /note="ATP binding site; other site" /db_xref="CDD:28956" misc_feature 14000..14002 /locus_tag="ClothDRAFT_2546" /note="Mg2+ binding site; other site" /db_xref="CDD:28956" misc_feature order(14090..14092,14096..14098,14165..14167,14171..14173) /locus_tag="ClothDRAFT_2546" /note="G-X-G motif; other site" /db_xref="CDD:28956" gene 14511..14879 /locus_tag="ClothDRAFT_2547" CDS 14511..14879 /locus_tag="ClothDRAFT_2547" /inference="similar to AA sequence:KEGG:HRM2_16520" /note="KEGG: dat:HRM2_16520 NADP-reducing hydrogenase, subunit B" /codon_start=1 /transl_table=11 /product="NADP-reducing hydrogenase, subunit B" /protein_id="ZP_05430686.1" /db_xref="GI:256005731" /translation="MKSLAELEEIRKRTLDQINLRTSNHDIRVVVGMATCGIAAGARP VMNAFVEELNKRNLTNVAVTMTGCIGMCKLEPIVEVIDKDGSKVTYVKMTPEKVARVV AEHIVNGRVCTEYTIAEADK" misc_feature 14592..14825 /locus_tag="ClothDRAFT_2547" /note="Thioredoxin (TRX)-like [2Fe-2S] Ferredoxin (Fd) family; composed of [2Fe-2S] Fds with a TRX fold (TRX-like Fds) and proteins containing domains similar to TRX-like Fd including formate dehydrogenases, NAD-reducing hydrogenases and the subunit E of NADH:; Region: TRX_Fd_family; cd02980" /db_xref="CDD:48529" misc_feature order(14592..14594,14598..14600,14703..14711) /locus_tag="ClothDRAFT_2547" /note="dimer interface; other site" /db_xref="CDD:48529" misc_feature order(14604..14606,14619..14621,14712..14714,14724..14726) /locus_tag="ClothDRAFT_2547" /note="[2Fe-2S] cluster binding site; other site" /db_xref="CDD:48529" gene 14912..16705 /locus_tag="ClothDRAFT_2548" CDS 14912..16705 /locus_tag="ClothDRAFT_2548" /EC_number="1.6.99.5" /inference="protein motif:PRIAM:1.6.99.5" /note="PFAM: Respiratory-chain NADH dehydrogenase domain 51 kDa subunit; 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: dat:HRM2_16600 NuoF" /codon_start=1 /transl_table=11 /product="NADH dehydrogenase (quinone)" /protein_id="ZP_05430687.1" /db_xref="GI:256005732" /db_xref="InterPro:IPR001450" /db_xref="InterPro:IPR001949" /db_xref="InterPro:IPR011538" /db_xref="InterPro:IPR017900" /translation="MQLYRAHVLVCGGTGCTSSNSNKIITELEEQIARNGIQNEVKVV RTGCFGLCAEGPIMVVYPEGAMYTMVKVEDVKEIVEEHLVKGRIVKRLLPGGGGKDEK ASLEGNDFFAKQQRIALRNCGVINPENIDEYIAFDGYKALAKVLTEMTPEQVVDVIKR SGLRGRGGGGFPTGLKWEFAMKQDADQKYVCCNADEGDPGAFMDRSVLEGDPHSVIEA MAIAGYAIGANQGYVYVRAEYPIAVKRLGIAIQQAREYGLLGKNIFGTDFSFDVDIRL GAGAFVCGEETALMTSIEGHRGEPRPRPPFPAVKGLWQKPTLLNNVETYANIPQIILK GPEWFASIGTEKSKGTKVFAVGGKINNTGLVEIPMGTTLREVIYDIGGGIPNGKKFKA AQTGGPSGGCIPASHIDTPIDYDSLTQLGSMMGSGGLIIMDEDTCMVDIAKFFLEFTV DESCGKCPPCRIGTKRMYEILERITEGKGEEGDIEKLEMLAKNIKASALCGLGQTAPN PILSTLRYFRHEYIEHVRDKKCAAGVCKALMHYEIDAEKCKSCGICARQCPVKAISGE KKVPYVIDQNKCIKCGVCMEKCPFKAISKKA" misc_feature 14927..15115 /locus_tag="ClothDRAFT_2548" /note="Thioredoxin (TRX)-like [2Fe-2S] Ferredoxin (Fd) family; composed of [2Fe-2S] Fds with a TRX fold (TRX-like Fds) and proteins containing domains similar to TRX-like Fd including formate dehydrogenases, NAD-reducing hydrogenases and the subunit E of NADH:; Region: TRX_Fd_family; cd02980" /db_xref="CDD:48529" misc_feature order(14930..14932,14936..14938,15044..15052) /locus_tag="ClothDRAFT_2548" /note="dimer interface; other site" /db_xref="CDD:48529" misc_feature order(14942..14944,14957..14959,15053..15055,15065..15067) /locus_tag="ClothDRAFT_2548" /note="[2Fe-2S] cluster binding site; other site" /db_xref="CDD:48529" misc_feature 15239..16489 /locus_tag="ClothDRAFT_2548" /note="NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit [Energy production and conversion]; Region: NuoF; COG1894" /db_xref="CDD:32078" misc_feature 15422..15901 /locus_tag="ClothDRAFT_2548" /note="Respiratory-chain NADH dehydrogenase 51 Kd subunit; Region: Complex1_51K; pfam01512" /db_xref="CDD:144926" misc_feature 15965..16120 /locus_tag="ClothDRAFT_2548" /note="endonuclease III; includes endonuclease III (DNA-(apurinic or apyrimidinic site) lyase), alkylbase DNA glycosidases (Alka-family) and other DNA glycosidases; Region: ENDO3c; cl11430" /db_xref="CDD:187036" misc_feature 16226..16363 /locus_tag="ClothDRAFT_2548" /note="NADH-ubiquinone oxidoreductase-F iron-sulfur binding region; Region: NADH_4Fe-4S; pfam10589" /db_xref="CDD:151119" misc_feature 16532..16603 /locus_tag="ClothDRAFT_2548" /note="4Fe-4S binding domain; Region: Fer4; pfam00037" /db_xref="CDD:143826" misc_feature 16622..16693 /locus_tag="ClothDRAFT_2548" /note="4Fe-4S binding domain; Region: Fer4; pfam00037" /db_xref="CDD:143826" gene 16725..18473 /locus_tag="ClothDRAFT_2549" CDS 16725..18473 /locus_tag="ClothDRAFT_2549" /inference="protein motif:TFAM:TIGR02512" /note="TIGRFAM: hydrogenase, Fe-only; PFAM: hydrogenase large subunit domain protein; iron hydrogenase small subunit; ferredoxin; 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: sfu:Sfum_0844 hydrogenases, Fe-only" /codon_start=1 /transl_table=11 /product="hydrogenase, Fe-only" /protein_id="ZP_05430688.1" /db_xref="GI:256005733" /db_xref="InterPro:IPR001041" /db_xref="InterPro:IPR001450" /db_xref="InterPro:IPR003149" /db_xref="InterPro:IPR004108" /db_xref="InterPro:IPR013352" /db_xref="InterPro:IPR017900" /translation="MQMVNVTIDNCKIQVPANYTVLEAAKQANIDIPTLCFLKDINEV GACRMCVVEVKGARSLQAACVYPVSEGLEVYTQTPAVREARKVTLELILSNHEKKCLT CVRSENCELQRLAKDLNVKDIRFEGEMSNLPIDDLSPSVVRDPNKCVLCRRCVSMCKN VQTVGAIDVTERGFRTTVSTAFNKPLSEVPCVNCGQCINVCPVGALREKDDIDKVWEA LANPELHVVVQTAPAVRVALGEEFGMPIGSRVTGKMVAALSRLGFKKVFDTDTAADLT IMEEGTELINRIKNGGKLPLITSCSPGWIKFCEHNYPEFLDNLSSCKSPHEMFGAVLK SYYAQKNGIDPSKVFVVSIMPCTAKKFEAQRPELSSTGYPDVDVVLTTRELARMIKEA GIDFNSLPDKQFDDPMGEASGAGVIFGATGGVMEAAIRTVGELLSGKPADKIEYTEVR GLDGIKEASIELDGFTLKAAVAHGLGNARKLLDKIKAGEADYHFIEIMACPGGCINGG GQPIQPSSVRNWKDIRCERAKAIYEEDESLPIRKSHENPKIKMLYEEFFGEPGSHKAH ELLHTHYEKRENYPVK" misc_feature 16734..16946 /locus_tag="ClothDRAFT_2549" /note="2Fe-2S iron-sulfur cluster binding domain. Iron-sulfur proteins play an important role in electron transfer processes and in various enzymatic reactions. The family includes plant and algal ferredoxins, which act as electron carriers in photosynthesis...; Region: fer2; cd00207" /db_xref="CDD:29262" misc_feature order(16818..16823,16830..16832,16857..16859,16863..16874, 16911..16916) /locus_tag="ClothDRAFT_2549" /note="catalytic loop; other site" /db_xref="CDD:29262" misc_feature order(16830..16832,16863..16865,16872..16874,16914..16916) /locus_tag="ClothDRAFT_2549" /note="iron binding site; other site" /db_xref="CDD:29262" misc_feature 16977..17099 /locus_tag="ClothDRAFT_2549" /note="NADH-ubiquinone oxidoreductase-G iron-sulfur binding region; Region: NADH-G_4Fe-4S_3; pfam10588" /db_xref="CDD:151118" misc_feature 17142..18263 /locus_tag="ClothDRAFT_2549" /note="hydrogenases, Fe-only; Region: Fe_only_hydrog; TIGR02512" /db_xref="CDD:162896" misc_feature 18276..18455 /locus_tag="ClothDRAFT_2549" /note="Iron hydrogenase small subunit; Region: Fe_hyd_SSU; pfam02256" /db_xref="CDD:145420" gene 18726..19328 /locus_tag="ClothDRAFT_2550" CDS 18726..19328 /locus_tag="ClothDRAFT_2550" /inference="protein motif:PFAM:PF01613" /note="PFAM: flavin reductase domain protein FMN-binding; KEGG: gbm:Gbem_2036 flavin reductase domain protein FMN-binding" /codon_start=1 /transl_table=11 /product="flavin reductase domain protein FMN-binding" /protein_id="ZP_05430689.1" /db_xref="GI:256005734" /db_xref="InterPro:IPR002563" /translation="MAKQLWKPSTILNPVPVVMVSCCGREGKPNIITVAWAGTINSKP PMVSISIRKERYSYNLIKEKGQFVINLTTRKLAFAADFCGVKSGRDVDKFEVLKLTQE KASVVDVPMIKESPVNIECTVKDIIELGSHDMFIAQIEAVSVDESLIDKKGRLCMEKA DLICYSHGEYWSLEKTLGYFGYSITKRKEVLKRRLNKKDR" misc_feature 18756..19250 /locus_tag="ClothDRAFT_2550" /note="Flavin Reductases; Region: FlaRed; cl00801" /db_xref="CDD:186194" gene complement(19387..20559) /locus_tag="ClothDRAFT_2551" CDS complement(19387..20559) /locus_tag="ClothDRAFT_2551" /EC_number="1.1.1.38" /inference="protein motif:PRIAM:1.1.1.38" /note="PFAM: malic protein NAD-binding; malic protein domain protein; KEGG: malic enzyme ; K00027 malate dehydrogenase (oxaloacetate-decarboxylating)" /codon_start=1 /transl_table=11 /product="Malate dehydrogenase (oxaloacetate-decarboxylating)" /protein_id="ZP_05430690.1" /db_xref="GI:256005735" /db_xref="InterPro:IPR005829" /db_xref="InterPro:IPR012301" /db_xref="InterPro:IPR012302" /db_xref="InterPro:IPR015884" /translation="MDYRKESLRLHGEWKGKIEVIHKVPVSTKEELSLAYTPGVAEPC LAIQKDVNLSYEYTRRWNLVAVITDGTAVLGLGDIGPEAGMPVMEGKCVLFKRFGDVD AFPLCIKSKDVDEIVKTIKLISGSFGGINLEDISAPRCFEIERRLKEECDIPIFHDDQ HGTAVVTVAAMINALKLVNKKIEDIEVVVNGSGAAGIAVTRLLMSMGLKKVILCDTKG AIYDGRDNLNSEKALIAKISNLEKKKGTLEDVIKGADVFIGLSVPGTVTKDMVKSMAK DPIIFAMANPTPEIMPDEAKEAGAKVVGTGRSDFPNQINNVLAFPGIFRGALDVRARD INDEMKIAAAKAIASLVSDEELNPDFILPLPFDPRVGKTVAAAVAEAARKTGVARI" misc_feature complement(20116..20517) /locus_tag="ClothDRAFT_2551" /note="Malic enzyme, N-terminal domain; Region: malic; pfam00390" /db_xref="CDD:144108" misc_feature complement(19453..20082) /locus_tag="ClothDRAFT_2551" /note="NAD(P) binding domain of malic enzyme (ME), subgroup 2; Region: NAD_bind_2_malic_enz; cd05311" /db_xref="CDD:133453" misc_feature complement(order(19612..19614,19618..19620,19642..19644, 19705..19713,19765..19767,19777..19782,19912..19917, 19975..19986)) /locus_tag="ClothDRAFT_2551" /note="putative NAD(P) binding site; other site" /db_xref="CDD:133453" gene complement(20612..21568) /locus_tag="ClothDRAFT_2552" CDS complement(20612..21568) /locus_tag="ClothDRAFT_2552" /inference="protein motif:TFAM:TIGR01771" /note="TIGRFAM: L-lactate dehydrogenase; PFAM: Lactate/malate dehydrogenase; KEGG: hypothetical protein ; K00016 L-lactate dehydrogenase" /codon_start=1 /transl_table=11 /product="L-lactate dehydrogenase" /protein_id="ZP_05430691.1" /db_xref="GI:256005736" /db_xref="InterPro:IPR001236" /db_xref="InterPro:IPR001557" /db_xref="InterPro:IPR011304" /translation="MEMVKSRSKVAIIGAGFVGASAAFTMALRQTANELVLIDVFKEK AIGEAMDINHGLPFMGQMSLYAGDYSDVKDCDVIVVTAGANRKPGETRLDLAKKNVMI AKEVTQNIMKYYNHGVILVVSNPVDIITYMIQKWSGLPVGKVIGSGTVLDSIRFRYLL SEKLGVDVKNVHGYIIGEHGDSQLPLWSCTHIAGKNINEYIDDPKCNFTEEDKKKIAE DVKTAGATIIKNKGATYYGIAVSINTIVETLLKNQNTIRTVGTVINGMYGIEDVAISL PSIVNSEGVQEVLQFNLTPEEEEALRFSAEQVKKVLNEVKNL" sig_peptide complement(21470..21568) /locus_tag="ClothDRAFT_2552" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.985) with cleavage site probability 0.362 at residue 33" misc_feature complement(20624..21562) /locus_tag="ClothDRAFT_2552" /note="L-lactate dehydrogenase; Reviewed; Region: ldh; PRK00066" /db_xref="CDD:178836" misc_feature complement(20624..21547) /locus_tag="ClothDRAFT_2552" /note="A subgroup of L-lactate dehydrogenases; Region: LDH_2; cd05292" /db_xref="CDD:133428" misc_feature complement(order(20867..20872,21197..21199,21272..21274, 21320..21322,21437..21439,21449..21454,21515..21523)) /locus_tag="ClothDRAFT_2552" /note="NAD binding site; other site" /db_xref="CDD:133428" misc_feature complement(order(20849..20851,20858..20866,20876..20878, 20888..20893,20900..20902,21404..21409,21416..21421, 21425..21427,21482..21484,21497..21499,21506..21511)) /locus_tag="ClothDRAFT_2552" /note="dimer interface; other site" /db_xref="CDD:133428" misc_feature complement(order(20885..20887,20897..20899,21032..21034, 21197..21199)) /locus_tag="ClothDRAFT_2552" /note="substrate binding site; other site" /db_xref="CDD:133428" misc_feature complement(order(20702..20704,20708..20713,20801..20803, 20807..20812,20987..20992,20996..20998,21047..21049, 21053..21055,21059..21061,21068..21070)) /locus_tag="ClothDRAFT_2552" /note="tetramer (dimer of dimers) interface; other site" /db_xref="CDD:133428" gene 22097..22450 /locus_tag="ClothDRAFT_2553" CDS 22097..22450 /locus_tag="ClothDRAFT_2553" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430692.1" /db_xref="GI:256005737" /translation="MVKYLKRQEELVEEALSKDNCSDWESLRNYHKSQIEFLQHERLV HLLVTLSFGFFFLISVLAAAVSEKFEILLVSLLLLVLLIPYIAHYYKLENGVQRLYEL YNKIDEKCVRKNKTA" gene complement(22522..23769) /locus_tag="ClothDRAFT_2554" CDS complement(22522..23769) /locus_tag="ClothDRAFT_2554" /inference="protein motif:PFAM:PF00365" /note="PFAM: phosphofructokinase; KEGG: maq:Maqu_2402 6-phosphofructokinase" /codon_start=1 /transl_table=11 /product="phosphofructokinase" /protein_id="ZP_05430693.1" /db_xref="GI:256005738" /db_xref="InterPro:IPR000023" /translation="MSRLEGACIFGQSGGPTSVINASAAGVIQEALKQDCITAVYGAA HGIKGILEENFFDMSKEDPYELELLKTTPSSALGSVRYKLKDADEDETDYKRLLEVFK KYNIRYFFYNGGNDSMDTCNKVSKYMQKVGYECRVMGVPKTIDNDLWGTDHCPGYGSA AKYIATSIMEVYHDARVYDTGMITILEIMGRNAGWLTAAAALASYKGNGPDLIYLPEL SFDIDKFVEDVTRIYKENGKVIVAVSEGIKDKNGKYISEYGSDLAKTKDSFGHAQLGG LASTLANIVKEKTGAKVRGIEFSLLQRCAAHVASLTDVNEAYLAGQAAVQYAVEGKTD YMVAFERAEGPEYKCNIKLLNLSEVANTEKKVPLEWIKPDGAGLTEDFIKYALPLIQG ESRPPIEDGLPRFAKLKKVLARK" misc_feature complement(22654..23733) /locus_tag="ClothDRAFT_2554" /note="6-phosphofructokinase; Provisional; Region: PRK03202" /db_xref="CDD:179553" misc_feature complement(22759..23733) /locus_tag="ClothDRAFT_2554" /note="Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to PFK family that includes ATP- and pyrophosphate (PPi)- dependent phosphofructokinases...; Region: PFK; cl00204" /db_xref="CDD:185826" misc_feature complement(order(22861..22863,22870..22872,23035..23037, 23197..23205,23329..23331,23335..23337,23341..23343, 23410..23415,23419..23427,23527..23529,23629..23631, 23725..23727)) /locus_tag="ClothDRAFT_2554" /note="active site" /db_xref="CDD:29437" misc_feature complement(order(23410..23415,23419..23427,23527..23529, 23629..23631,23725..23727)) /locus_tag="ClothDRAFT_2554" /note="ADP/pyrophosphate binding site; other site" /db_xref="CDD:29437" misc_feature complement(order(22792..22794,22813..22815,22825..22830, 23056..23058,23143..23145,23161..23166,23254..23256, 23263..23265,23275..23277,23311..23313,23557..23559, 23566..23568,23590..23592,23683..23685,23695..23697)) /locus_tag="ClothDRAFT_2554" /note="dimerization interface; other site" /db_xref="CDD:29437" misc_feature complement(order(23056..23058,23062..23064,23137..23139, 23143..23145,23254..23256,23566..23571,23587..23592, 23683..23685,23695..23697)) /locus_tag="ClothDRAFT_2554" /note="allosteric effector site; other site" /db_xref="CDD:29437" misc_feature complement(order(22861..22863,22870..22872,22888..22890, 23035..23037,23197..23205,23230..23232,23329..23331, 23335..23337,23341..23343)) /locus_tag="ClothDRAFT_2554" /note="fructose-1,6-bisphosphate binding site; other site" /db_xref="CDD:29437" gene 24016..24945 /locus_tag="ClothDRAFT_2555" CDS 24016..24945 /locus_tag="ClothDRAFT_2555" /EC_number="4.1.2.13" /inference="protein motif:TFAM:TIGR01859" /note="KEGG: dde:Dde_2341 fructose-bisphosphate aldolase; TIGRFAM: fructose-1,6-bisphosphate aldolase, class II; ketose-bisphosphate aldolase; PFAM: ketose-bisphosphate aldolase class-II" /codon_start=1 /transl_table=11 /product="fructose-1,6-bisphosphate aldolase, class II" /protein_id="ZP_05430694.1" /db_xref="GI:256005739" /db_xref="InterPro:IPR000771" /db_xref="InterPro:IPR011289" /translation="MPLVTSTEMFKKAYEGKYAIGAFNVNNMEIIQGITEAAKEVNAP LILQVSAGARKYANHTYLVKLVEAAVEETGLPICLHLDHGDSFELCKSCIDGGFTSVM IDGSHLPFEENIKLTKQVVDYAHSKGVVVEGELGRLAGIEDDVNVSEADAAFTDPDQA EEFVKRTGVDSLAIAIGTSHGAYKFKGEAKLRFDILEEIEKRLPGFPIVLHGASSVIP EYVDMINKYGGDMPGAKGVPEDMLRKAASMAVCKINIDSDLRLAMTATIRKYFAENPS HFDPRQYLGPARNAIKELVKHKIVNVLGCDGKA" misc_feature 24034..24942 /locus_tag="ClothDRAFT_2555" /note="hypothetical protein; Provisional; Region: PRK08185" /db_xref="CDD:181275" misc_feature 24037..24936 /locus_tag="ClothDRAFT_2555" /note="Tagatose-1,6-bisphosphate (TBP) aldolase and related Type B Class II aldolases. TBP aldolase is a tetrameric class II aldolase that catalyzes the reversible condensation of dihydroxyacetone phosphate with glyceraldehyde 3-phsophate to produce tagatose...; Region: TBP_aldolase_IIB; cd00947" /db_xref="CDD:29573" misc_feature order(24094..24102,24109..24111,24169..24171,24178..24186, 24196..24198,24208..24210,24217..24222,24439..24441, 24784..24789,24793..24798,24805..24807,24817..24819, 24826..24828) /locus_tag="ClothDRAFT_2555" /note="intersubunit interface; other site" /db_xref="CDD:29573" misc_feature order(24259..24264,24550..24558,24562..24564,24646..24651, 24655..24657,24775..24777,24781..24786) /locus_tag="ClothDRAFT_2555" /note="active site" /db_xref="CDD:29573" misc_feature order(24262..24264,24553..24555,24646..24648) /locus_tag="ClothDRAFT_2555" /note="zinc binding site; other site" /db_xref="CDD:29573" misc_feature order(24550..24552,24556..24558,24562..24564,24649..24651, 24655..24657) /locus_tag="ClothDRAFT_2555" /note="Na+ binding site; other site" /db_xref="CDD:29573" gene 25042..25608 /locus_tag="ClothDRAFT_2556" CDS 25042..25608 /locus_tag="ClothDRAFT_2556" /EC_number="3.4.21.89" /inference="protein motif:TFAM:TIGR02227" /note="KEGG: dde:Dde_2816 signal peptidase I; TIGRFAM: signal peptidase I; PFAM: peptidase S24 and S26 domain protein" /codon_start=1 /transl_table=11 /product="signal peptidase I" /protein_id="ZP_05430695.1" /db_xref="GI:256005740" /db_xref="InterPro:IPR000223" /db_xref="InterPro:IPR011056" /translation="MNTNMDNFETAGNGNSKKQKVFKEIVSWSLCLLGAFIIALLLTK YVIVNAYVPTGSMENTIMPGDRIIASRIHYYFSEPKRGDIVVFRYPDNEEVLYVKRII GLPNETVEIKDGNVYINGKLLEEPYIKEKAYGDFGPYEVPEGCYFMLGDNRNGSTDSR RWTNKYVKKEKILGKALFKYFPGFKILW" misc_feature 25123..25587 /locus_tag="ClothDRAFT_2556" /note="signal peptidase I, bacterial type; Region: sigpep_I_bact; TIGR02227" /db_xref="CDD:162773" misc_feature 25189..25569 /locus_tag="ClothDRAFT_2556" /note="The S26 Type I signal peptidase (SPase; LepB; leader peptidase B; leader peptidase I; EC 3.4.21.89) family members are essential membrane-bound serine proteases that function to cleave the amino-terminal signal peptide extension from proteins that are...; Region: S26_SPase_I; cd06530" /db_xref="CDD:119398" misc_feature order(25207..25209,25336..25338) /locus_tag="ClothDRAFT_2556" /note="Catalytic site; other site" /db_xref="CDD:119398" gene 25879..27270 /locus_tag="ClothDRAFT_2557" CDS 25879..27270 /locus_tag="ClothDRAFT_2557" /inference="protein motif:PFAM:PF00639" /note="PFAM: PpiC-type peptidyl-prolyl cis-trans isomerase; KEGG: aci:ACIAD1409 peptidyl-prolyl cis-trans isomerase precursor (PPIase) (rotamase)" /codon_start=1 /transl_table=11 /product="PpiC-type peptidyl-prolyl cis-trans isomerase" /protein_id="ZP_05430696.1" /db_xref="GI:256005741" /db_xref="InterPro:IPR000297" /translation="MVHKKKTAVITGVAVLVGIVIIALAVGYNYYSKRNKPQESKEFG FQALKINGTYVSTDIMKEERNKFFEKYKRNADVLRMNDHERNDMLLDQVIERLLLEDY VNNKSGVTATDSEVEDYINRFIKPRYGDSLGTFMSSQGYTNEEEMKAGIKEYILKHKA LYKAAKEKNVTLTEQELDEGYEKHKIQNKKVDIRHIFISSQERGKEEAKKLADEIYNR LKNNEDFETLAKQYSDDEKTKESGGVITELRAGFNEAVFDNAVFTAEAGQLLEPIEVA RGYEIVYVDKVTDFYRTRDEYAELLTVDKFMQSDAYKEWFEEYKKNYDIEITDPAMKA FRLFREKQYNEAGALYEELYKSEKDAYYIEMACEAYKLAENWAGLIEAGKLAVKENPD FVNYYLYQAEGEFKVGDANKAKELLKEAEKKAGDNTYFLDLIRKMYESQGLAEDVERI DRKFQEISEKLKG" sig_peptide 25879..25956 /locus_tag="ClothDRAFT_2557" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.991) with cleavage site probability 0.946 at residue 26" misc_feature 26143..26904 /locus_tag="ClothDRAFT_2557" /note="peptidylprolyl isomerase; Reviewed; Region: prsA; PRK02998" /db_xref="CDD:179522" misc_feature 26461..26736 /locus_tag="ClothDRAFT_2557" /note="PPIC-type PPIASE domain; Region: Rotamase; cl08278" /db_xref="CDD:186815" gene 27329..28321 /locus_tag="ClothDRAFT_2558" CDS 27329..28321 /locus_tag="ClothDRAFT_2558" /inference="protein motif:COG:COG3580" /note="KEGG: sfu:Sfum_1829 putative CoA-substrate-specific enzyme activase" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430697.1" /db_xref="GI:256005742" /translation="MDVKVGIPRALFYYQYYPLWKTFFEELDAQVVLSEHTTAKILDE GIKSCVSEACLPVKIFYGHVMNIKDKVDYLFIPRFTSISKKEYICPKFGGLPDMIRNS IKGLPPLIDTEINLRKSKKNAIKAAMETGGFFTKDKRKIERAYKKALESYREFNEKIK QGALPSDILDRKLNVIKKSNEPELNILVIGHVYNLYDSFINMDLIDKLRKNGVKIMTI DMVEEDIARKNAGLLPKKMFWNFGSRAFGSVMNFLDKEDIDGIIYLMSFGCGVDSFVC DLIERRVRKTKDVPFIILTLDEHSGQAGMDTRLEAFIDMVRWRYRNENNVSAYG" misc_feature 27338..27991 /locus_tag="ClothDRAFT_2558" /note="CoA enzyme activase uncharacterized domain (DUF2229); Region: DUF2229; cl12083" /db_xref="CDD:159743" gene 28293..29408 /locus_tag="ClothDRAFT_2559" CDS 28293..29408 /locus_tag="ClothDRAFT_2559" /inference="protein motif:COG:COG3581" /note="KEGG: sfu:Sfum_1830 CoA enzyme activase" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430698.1" /db_xref="GI:256005743" /translation="MKITFPHMGNTYIAVKALLDDIGAEYVIPPLNSKRSLELGTKYA PEMACLPLKINIGNYIEAYEKGADTILTAGGRGPCRFGYYCEMCREILNDNGYTMDVI VLEPPDVEIMKFIGKIRKLAGGLNIYHILKVIKNTTIVAQRVDELERLAFKIRPRELR KGSTDRIYEGFRRKVIDVKGSQEILKLVEDTKNQLLKLEIDKDARPLKIAIVGEIYTT IDSYTSFNIDSILGGMGVEVHRANTISGWIIEHILKAVVPFTKDKRYAEAAKPYLGTM IGGHAQETIGNTVLYAKDGFDGIIQIYPLTCMPEIVAESILPAVERDYNIPILTLIID EMTGEAGYMTRIEAFVDLLRKRRERGEIAENLVLSGN" misc_feature 28293..29378 /locus_tag="ClothDRAFT_2559" /note="Uncharacterized protein conserved in bacteria [Function unknown]; Region: COG3581" /db_xref="CDD:33381" misc_feature 28293..29369 /locus_tag="ClothDRAFT_2559" /note="CoA enzyme activase uncharacterized domain (DUF2229); Region: DUF2229; cl12083" /db_xref="CDD:159743" gene 29377..30366 /locus_tag="ClothDRAFT_2560" CDS 29377..30366 /locus_tag="ClothDRAFT_2560" /inference="protein motif:TFAM:TIGR00241" /note="TIGRFAM: CoA-substrate-specific enzyme activase; PFAM: ATPase BadF/BadG/BcrA/BcrD type; KEGG: gur:Gura_1539 putative CoA-substrate-specific enzyme activase" /codon_start=1 /transl_table=11 /product="CoA-substrate-specific enzyme activase" /protein_id="ZP_05430699.1" /db_xref="GI:256005744" /db_xref="InterPro:IPR002731" /db_xref="InterPro:IPR008275" /translation="MLKTSYYLGIDVGSVSTNLVVINENDEIVEKLYLRTSGQPINAL KNGMKTLYQRLGKDVKIRGVGTTGSGRQLASVIVGADIVKNEITTHAIAAQKLVPEVR TIIEIGGQDSKIIILKNGIIYDFAMNTVCAAGTGSFLDRQAARLEIPIEEFGSFALRS KTPVRIAGRCAVFAESDMIHKQQTGHSVEDIINGLCEALVRNYLNNLAKGKDIEEPIV FQGGVAANVGIVAAFERAIGKKIIIPQHYDVMGAYGAALIAKEEMMKNGKNTNFFGFD NIHNDFRARSIECNGCSNMCEVIEIVSNDAVVACWGDRCGKWSAVKKENQSVG" misc_feature <29392..30159 /locus_tag="ClothDRAFT_2560" /note="ROK family; Region: ROK; cl09121" /db_xref="CDD:186831" gene 30437..31615 /locus_tag="ClothDRAFT_2561" CDS 30437..31615 /locus_tag="ClothDRAFT_2561" /inference="protein motif:COG:COG1379" /note="KEGG: lip:LI0706 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430700.1" /db_xref="GI:256005745" /translation="MKEYFVDLHVHIGRSSKGKEVKKATANNLTFENIAFESYTRKGI DVIGVVDCLSPYVIEDIEELVDRGELIEKQGGGMEYRKGQILILGAELETHEEKGGSS HSLCFFPTLKSIKDFAKAMENHIKNIYNCSYMCRLTARQLIDIVDFYGGTFIPAHVFT PYKSFYGNCCDSLFEIFDEKSFDKIPAVELGLSADSMMASQLGELDEKAFLSNSDAHS LSKMGREYNIFEMEEANYEEILKAFKGIDGRKIKANFGLDPKLGKYHRTYCLVCDSVI KEDPPVLKCPVSDKHRVVVGVKDRLMMIRDRENPLMDSRPPYFYQVPLEFLPKVGPKT IDKLIEFFGSEMRVLHYASYEELTKVVSEDIAKNIVLSREGKLSIEAGGGGVYGKIEA " misc_feature 30446..31609 /locus_tag="ClothDRAFT_2561" /note="PHP family phosphoesterase with a Zn ribbon [General function prediction only]; Region: COG1379" /db_xref="CDD:31569" ORIGIN 1 atatttcaaa acaagattat ggaaagatat ggataattat aaaaacaaat aaattcatta 61 aaagcaaata aatatattat tgaaaaaaac ttctagaaag tataatatta tagtaaccag 121 tcaaaaagta ttgatatatg cgtacgaaaa cttaaaagga gtggtagcaa aaatgaagaa 181 aatttggggc agaaaagttt tgggatttgc ggttggtgta tgcttacttt taactatgat 241 ttgccaaaat gtggcttttg tatcggcaga acctgaggaa aaaactgttg tcgtaaattt 301 cgaagaaagc ttgaatgaaa ctatgtcaaa aaccattgaa atactaaatc tttttgacat 361 ttccgagatt gttgtcgata gcggaaaagt gtcttacagc agagaaggag acaaagtaac 421 ggttactgtt tcggaaggcg tttatagagt aggcccgcat acacaaaatg ttactttaac 481 tatagaggat gataaaggta tttttgatga gaaaacatcc tataatgttg acggctatga 541 aggaatcctt acgaaaatag attctggtta tgatgaggca aaaggactgt actgggtaaa 601 atatcaaggt agtgtaacta aagaaaactg caaattttac caatatacag taacaatcaa 661 atatacggaa aatgttcgtc cagaagtgta tcttactgag ccgaaacgag gcatgattac 721 taatggtaag attaatgttc agggttatgt cagagatgaa aacataggtg atgagctgaa 781 attgttcttt agctttgaca gttacgatga aagcatgaca ggaaatcttc tgaatgagaa 841 ttccattata tccgacggaa cctggcagga gataagcgga actatagatt tatcaccttt 901 caatcttaaa gacggcgatc acgactttta tttttgggca gtggataaaa ggggagtaag 961 atcggttggc gagatcataa ggtttacgct tgatacagta ccgcctgaag cgcctgttct 1021 tactccggat aaaacagagt ctacgaatca gagtgttgta gtgtcagtat attatccacc 1081 ggatgctgta ggcagggaga taaagataaa tgacggacct tggatgccga taaccgatat 1141 aaccaaaaat gatcagataa taatggatga gaacggcaag atagaggcaa gggcaattga 1201 tgaagcggga aatatttcag aggtggcgga acttgagata aagaatattg acaaaattcc 1261 tccgacagca ccgacaatca atacaagtgc tgatgaaact acagagcaac cgattaaggc 1321 gacgatagtg ccgggagttg ataatgagtc aggtgtggat cgaaccgaat attgtttaag 1381 aggagcaagt acaaaagatt gggagaaata cgatgaagga accgaaataa caataactgc 1441 gttgggagaa acagaaattt gtgcaagaac aattgacaat gccggaaaca tctccgctga 1501 aacagttaag aaagttacaa taaagaagaa agaggacagt ggcggtaaca acggtggaag 1561 tggcggcaca ggcggaaaca gcggtaacaa cggtggcagc ggtggcacag gcggaagcgg 1621 tagtagcggt ggaagcggaa gtaacagtgg tggcggaaat aatgacggaa atggcaacga 1681 cgggaaaaaa gacgatgaaa tactgcagcc cgaacccaat attccaggcg caggaggcag 1741 tcctgtggat ttgtccgtgt ttataagtgc ggataaatca aaatatgaag aaggtgaagt 1801 aattactttc aatattacat acaaaaacaa aaccaatgtt caggcaaaca acgttattgt 1861 gaaagcagga ataccggcaa acacaactgt tgaggatata gccggaggta ctcaaaatgg 1921 aaatgacatt gaatggaaaa ttgaatcgct taaagcaaac tcttcaggca agattcaata 1981 caaagtcaag gtgaatttgc ttgaggtgcc ggaaataagt tcttctgcta ctgcttcaat 2041 aactgcaagt ggaactctta ttaacaagga tgacgatgaa tcaagaacta tattccttct 2101 ttattcgaac cgttttggtg aaaactttca cggcaaatat attacaggct atgaggacaa 2161 tacattcaga ccgttgaata atataacaag agctgaagtg gcaacaatta tggccaacat 2221 tttgggattg aagcaggagg ttgcaggagg caaaacatat acagatttgt caaagagcca 2281 ttgggcatat aacaatataa ttgcggtaac cgaaaaaggt ttgttcacag gatatgaaga 2341 cggttcgttc cgtccggaca actttatcac aagggcggaa tttgctacgg tgctggctaa 2401 ttatttggga cttaagaatg ttgagcatga tgagttgaac tttgcggata tcgaaaatca 2461 ctgggctaag aactttatag aggaaatata cagagtaaga ttgatagaag gttatctgga 2521 aaatggctta agactgttta agcctgacaa ctacataacc agaagtgaag cggtgacaat 2581 aataaacaag atgctgttca gaggtccgct tgaaggagca aaggtgccgt ttaccgatgt 2641 tgaggaagga tactgggctt acggacatat attggaaagc tctatagatc attactacgt 2701 aagaaataaa gatcagagcg aaacaatagt aaacaagaaa cagtaaacaa ggaacgtcaa 2761 acagaagaga acggcgtagg ggcaatcttt aggaaaattc aattgaaaga ttctaaaata 2821 cagcttgctg aaaataaact tatgaaaaaa tgagggacgg ttgtaacagt cgtccctcat 2881 ttttaataag aagacttaat aaaatttttt accttgtata aaacattaca gcaaatctct 2941 ttttgcaatg tcctcaagtt tcagcgaagg atttttttct tcagcccacc gtatggacca 3001 ttcgttttca aacaacagaa catatctttc atcctggtca tgaacaatca ttgttgtgct 3061 cgttaacgtt aagtttctgg gctccgtgtc actggacaca atccagcggg catgcctgtg 3121 aggaagtttc tgaattctta aatccacgcc gtactcgtgc ttgagtctgt actccaaaac 3181 ctcaagctgc agaattccga caactccgac gataatggac tcaaacccta tgtcaatttg 3241 tttgaaaacc tgtattgtac cttcctcaga gagctggttg atacctttta taaattgctt 3301 acgtttcatg gtgtctaccg gagtaacttt ggcgaaatgt tcggccggga atattgggat 3361 tccttcaaat ctaagattgc tgttgccttc gcttaaagtg tctccgatgt ggaatatacc 3421 gggatcgaac acacctatta tatctccggc ataagcttcc tcaacaatgg tgcgttcctg 3481 cgccataaac tgttgtggct gggaaagacg cacttctttt ccctgttgaa cgtggtaaac 3541 tgtcatcccg cgggtaaatc gtccggaaca gattctgatg aaggctattc tgtccctgtg 3601 ggcgggattc atgttcgcct ggattttaaa cacaaatcca gtaaacttgt ccgattcagg 3661 gtcaacttct ccttcggaag acatctttat gccgggtttg ggtgccagct gtaaaaactc 3721 ctcaagaaaa ggctgaactc cgaaattggt catggcactt ccgaaaaaca ttggagtaag 3781 ttcacccttc aatatttttt ctttgtcaaa aggatctccg gccatatcaa gaagctctat 3841 atcttcacag agctttttgt ggtaatgatc tccgagaagg tctgcaaata tcttgtcatc 3901 aacgctgcct ttaattgagg agacaacggt ttggccgtga ttgccgcctt caaacaattc 3961 tatctgggaa agctttctgt tgtatactcc cttaaaatct ccgtcggttc ctatcggcca 4021 attcatggga taggagcgga tcccgaggac attctccagc tcttccatga gttcaaaggg 4081 gtccttgctg gctctgtcca ttttgtttac aaaagtgaat atgggaatac ccctcatttt 4141 gcaaacatga aagagcttga ttgtctgttc ctcaactcct ttggcaccgt cgattaacat 4201 gaccgcgctg tcggcagcca taagggtacg gtaggtatcc tcactaaagt cctggtggcc 4261 cggagtatcc agtatattaa tgcagtagtt gttatattca aattgcaaaa cacttgaggt 4321 aaccgagata cctctctgtt tttcaatctc catccagtcg gatgtagcat atttgtttgc 4381 ttttctcgct ttgaccgacc cggccagtct tatggcacct ccatacagaa ggagcttttc 4441 cgttaaagtt gttttaccgg catcggggtg ggatataatt gcaaacgtcc ttcttctttc 4501 tacttcgtaa ctgattttat tttgcatgat agcataacct cttaccgtta attattaatt 4561 tattatgtat tataaggcaa caatttattt tatattattg ccgcgcggca tgtcaataat 4621 gacgatagaa gggaacgtgc aatatacttt ttacagattg ccaattcaac cacatatatt 4681 ttgttttagc atgcggattg ttagtgggac aatttccaag tttggaaatg gtaaaagagt 4741 aaatcttctt gattctagtt aatgtcctat ttgcgcatta tttcgaagca ttttagtctg 4801 ttgtttggtt atgctttcga acgacttaaa gagataccta gggtacaaaa atattaatat 4861 ttaataaaga ctgattattg aattctcgag atgtgtagtg ggttatttat gtaccaagtt 4921 gtagtaaata ttttcaatta agtgacgata atttgaatag aaaaattcaa agattatatc 4981 tttttgttta cttttaatac tttgtatgat tatgtttgat aaatttgtaa aagaattttg 5041 aaaggcctta attaaaagaa attcatataa atgatgaggg tgataaggat ggcacttaca 5101 aaagaacgtc ttttggaaga aatatctgaa tcttcggggt tttcaactgt atttaatagt 5161 tgtagtcgag aattacagaa tttgttgata aatctagtta tcgaaatttc aagatactct 5221 tgtaaccgag aagggtatgt taaaaatatg aaagaaacaa gtataagatt tgaaaaacca 5281 tatttagttg gaagaaaaaa tcaaaattat tgtatgttaa ctttgcgacc acgattaaat 5341 caaattgtag tagacgttag gacagacggt aagtttatta attcagaaac tcttaaacta 5401 attaatcttg gcaataaata taatggagga tttgaatggc atcgctttgt tgttaaagac 5461 gaaaacgaga ttaaggaagc agtaagattg atttcgaaat gttatgaggg ttaaagctgg 5521 tattgttata tttttgaaag aaaaaaattc taataaaatg tatttcaaaa tatatataag 5581 aaaagaaaaa tgaaaactct ttttattttt caaaattttc aaagataata ataagttgat 5641 tgctaggtaa aaggtgttat ataatcaaaa atagatgagg tttatttgat attattgtat 5701 ataaacaaaa aattacttat aggggacttt aaaatcaggt gcttgagata ataagctgca 5761 gcagaagaac ggacattccc gccttttatt atgactggct tcaggaatgc ctgaaaaata 5821 aatatgcagt ggtaaaaaat ccttacaata aatccactta catggtggat ttatcttgtg 5881 aaaaggtgca ttcaatttgc ctgtggtcca agtcctttgc caatgtactc aaagacccga 5941 aatatttatc gctgtacaat ttgtattttc agtttacaat cacaggatat tcaaaatttt 6001 tggagccaaa tgttattgat acaaacgagg cagtcaggca gatggaacaa ctggctcaaa 6061 agtattcacc gcggcagatt aactggagat ttgaccctat tatttttagt gcggagggag 6121 aaaaggaacc aacaccggat aactttgaaa gagcgagatt aaaagtattt gagaatttat 6181 gcaaagatat ttcatccttt ggagtcaatc gctgtaccat aagctttctt tgtttatata 6241 aaaaagtgga gcagagaatg aaaaaatgca attttactta ttttccgcct tcacagcaaa 6301 agcaaattga atttgtaagc cggttggttg agattgctga taaataccaa gttactattt 6361 atacatgcag cagtccggtt attgaatcgg tgcccggggt gaaaaaggga cattgtatag 6421 acggagctta ccttgaagaa ctattcggaa aacgggcctc gagggcaaag gactccggac 6481 agagaaaaga atgcggatgc accaggtcaa aagacattgg aggctatgat aatcaaagtt 6541 gcaggcatgg atgcgtgtat tgctatgcaa gatagttaaa ttatgatata tttaattgta 6601 aatattataa agtagtgaat ttataagcag atgttataaa gatattattg atagaaagaa 6661 gaataaaggg aatacagcaa tattgttaat gttttataac gggagaggct tatatggata 6721 aaaaatatac agaaataaat tccaaggtta ttgataaatg ggtggaagaa ggttgggaat 6781 ggggataggc tgttagccat gaggtgtttg aaaaggctaa aaatgacgat tggcatgtgc 6841 ttctgacgcc tacaaaacct gtgccaaagg attggttttg cgaaataaag aatgccgaaa 6901 ttttgggact ggcatgtggc ggtggtcaac aaatgcccat atttgcagca ttgggcgcaa 6961 aatgcacggt gctggattat tcggaaatga aagatgagca attgtaccgg gagtcaataa 7021 aaaataattg gggaattcag ttttcgcata ccatagaaga acaaattgga gggcaactgc 7081 gggcaggatt tattcttacg gatatatatc aggacacaaa tggttttggc aggcttcacg 7141 aattcaatgt cccgacttat tatgcaacaa gggcaattaa aatataagaa gcaacttgaa 7201 cgtcggtatg aaaacttgaa gccgaaaatt atgaagtaga aaatatatag tttatcacaa 7261 gaggatgtgt ttataatggt tggagtagaa attgatatga ttgtggcgga cagtttgaaa 7321 gcactggaat tgtacgagaa aatatttgat attcagcgtg ttgaggtatc caatcttcca 7381 aaaggtgaaa atgaagttgt ttttactttg tacggagtgc gctttcacat gctggacgaa 7441 aatccggaat tcggtttaaa agcaccggat cgtgacagac ctaatacaat ttggtttaac 7501 gtgtcggttc ctgatattaa agaaacattt tcaaaggcga taaatgcggg ttgcacagag 7561 atacagccgg cgaccgaaat gcctgattac ggattgtcaa atgccatatt tgccgaccct 7621 ttcggatatc aatggatgct gcatcaaata caaagagaag taagttttga agagcgcatt 7681 aggctttggg aggattcgcg caaagaaaaa taaattattt ctaatataat aataatgcta 7741 aaaattaaaa tgctaaaata atattgaaaa tatttttgtg aagcaaccgg aaacggtgat 7801 attgcctttt aatgaataca tggagattag ggacttaaaa caagagtgtt tggatagatt 7861 taaaaaatca aaagagtgtt attattatcc ggcaggagac ataaagtttt aatatactcc 7921 tgcttaattt atatgtgaaa atgtgataat atgataatac aaatagaatt atcgaatgaa 7981 taaaatagat aaagtatcat gcttacaaac ataagaaact tgtagaataa gcttgcagat 8041 caaacaagtt tgacgcagaa actatttaat tatacaggaa catcttgctt tattacatta 8101 taatatttaa aagaggaatt ttgcttttgt ttatagaata attctattaa aactcgaaat 8161 aatcaacata aaatattgca taaggagtta taggagatat gaatgaaaac aatctaaata 8221 tgattaaagt ggtatttccg gataacagcg aaagagaagt gtatgaagga atatctttgc 8281 aggaattgag tgaaagctgc aaaaaccaat ataaatcaac cattgtggcg gcaaaggtta 8341 acaacgatat aaaggaatta agctatcgtc ttaatgaaag ttgccgggtg gagtttatcg 8401 accttacgga tgatgacgga atgaggatat ataaaagaag cctcagcttt attctcatta 8461 aggccgtaaa tgaccttttt cccgacagaa aggttataat ttgccattcc atcagcaagg 8521 gaatttactg tgaggttaaa ggcgacacac ctcttactgt tgaagaggta gacatgataa 8581 aaaacagaat gaaagaaatt gtcaatttga aaattccttt cataaagaag ataatgtctc 8641 ttgatgaggc aagggaagta ttcagaaaaa tcggaagaat ggacaggttc cgttccatag 8701 aatacagaaa aaagccctat gtgactttat acgaatgcga tgggttccag gactattttt 8761 atggatatat ggtgcctcat acggggtatc tggataaatt tgatttaaaa tattatcagc 8821 ccggcctgat attgatgagt ccggaaaaaa ccagtccgga tgctataccg caattcaaag 8881 agcaaaagaa gcttttcagc atatttgcgg aatacaaaaa atggggaaaa atacttggtg 8941 tcgaagatgt gagtgcgcta aatgacattg taaaggaagg caaaataaat gagcttataa 9001 gagttgcaga ggctttgcat gagaagaaaa ttgcgcagat tgcggatatg atagccttta 9061 atgagcataa gaagaaagtc gttttgattg ccggtccgtc ttcatcggga aagacaacct 9121 ttgcccacag actttcgata cagcttaagg taaatggttt gaggcccgtt accatatctc 9181 tggatgatta ttttgttgac agggagctta ctcccaagga tgaaaacgga gaatacgact 9241 ttgaggccct ggaggctatt gatatcaaac ttttcaaccg gcatcttgcg gagctgatag 9301 aagggaaaga agttgacgtt ccgattttca atttccctaa aggatgcagg gaaagctttt 9361 gcaggaagct taagattgac gaagaccaga taatcataat tgaaggcata cacggattga 9421 atgaaaaact gacggcctca attccaaaag agaacaaatt caagatatat gtgagcgcac 9481 ttacctcaat gaatattgat gagcataatc gtatacctac tacggataca cgaatcatca 9541 gaaggattgt aagagattac caattcagag gctgcagtgc ggcaaacact ataaaacgct 9601 ggccttctgt aagaaggggc gaggagagaa acatattccc gttccaggaa gaagcggatg 9661 taatgtttaa ttcagcgctt atgtttgaac tgggagtttt aaaaacctat gcggaaccgc 9721 tgctgatgga gatagattct tctgagcctg aatattccga ggcaaggaga cttatagaat 9781 ttttgaacaa tttcttgccg atagactcga aagagattcc tgcaaattca ataataaggg 9841 agtttattgg cggaagttgt ttttaccagt aaataaaccg taaataaagc ataaataaag 9901 tatagataaa gcatgaataa accataaata atcgtaaata actgttaatt gaaaaccatt 9961 aataaatctt gtttgataca aaatgtaaag gttaaggtgt atgtatgaag gctagggaga 10021 ttttagaaat tttaaatgca gaacttttga ccggggaaga aaatcttgac atggagttta 10081 atgcagcctg tgggtcagac cttatgagcg atgttatggc ctatgtcaaa gaaaatgtgg 10141 ttttgctgac agggcttgtc aatcctcagg tgattaggac tgcggaaatg atggatataa 10201 aattggtggt tttcatcagg ggaaagaggc ccgaggagaa gattattcaa atggcaaaag 10261 aaaaagggat agcaatcatg accacgaacg acccgatgtt tatagcatgc ggaaagttgt 10321 acagtgcagg tataacagaa agaggtgttt gggattgagt gacggtataa taaggaaaaa 10381 ctttgttata cctgcaaatg attttacatt ggccggggaa gcatcaagca ccgtaaagaa 10441 gatgcttata cagcttggtg ctgaaccaca gaaaataaaa aagacggcca tatccatgta 10501 tgaggctgaa ttgaacgccg tcattcacgc caacggcgga gtggcggaag ttgaaattga 10561 cagaaacaaa gtctttatac ggataaaaga tgaaggaccg ggaatacctg atttggagct 10621 ggcaatgcag gaaggatata ccactgctcc ggacagtatc cgtgaaatgg ggtttggtgc 10681 cggaatggga cttcccaata tgaaaagata tgctgacaag cttgagataa taacggaagt 10741 aggaaagggg actattgttg agattaccgt gtttttaacg gataatgaga aagagaacca 10801 ttaacgtgtt tggggggata agatgagtcc gtacttccac tcggtgactc tggacgaggt 10861 caaatgtaaa ggttgtacca attgcataaa aagatgcccc acggaagcaa tcagagtaag 10921 aaagagcaaa gccagaatca taaatgaaag atgcatagat tgcggagagt gtataagggt 10981 ttgtccttat catgccaaaa aggcgataac cgaccctatt ggcctgataa atgactataa 11041 atacaaagtt gcgatacctg ccccttccct gtatggacag ctaaagtcag cgccttcaag 11101 ggatcatatc ctcactgctc ttaaaatgtg cggatttgac gatgtttttg aggttgcaag 11161 ggcagccgag attattacca gtgagactaa aaaaatactt gcacagcaaa gatttgaaaa 11221 gccccttata tcttcagcat gccctgctgt agtaaggctc atacaagtga ggtttccaaa 11281 tcttatcgat aatatattaa agctaaagtc ccccatggag gttgcggcca aaatcgcaaa 11341 agatgaaata tcacgaaaca ggaaaattcg ccaggaggac atcggcgtat tttttattac 11401 tccctgtgcc gcaaaagtga ccagcataaa agctcctttg gacaaggaga agtcctttgt 11461 ggacggagca atttcaattt cggaaatata tttaaaaata ctttctgccc tggggaaaat 11521 tgataaacct gaaaagcttt caagagccgg gtttgtcgga gtacgctggg caaattccgg 11581 aggagaaagc atcgcccttg gaacggacaa atttcttgcg gttgacggta ttcacaatgt 11641 aattgcgatt cttgaggaaa ttgaggacga aaagctggag gatatagatt ttgttgaagc 11701 tctggcatgt cagggaggct gtcttggcgg tccacttaac gtggaaaata tgtatgttgc 11761 cagaaaaaca ataaagaaat ttattgatga tgcaaaagaa aaagggttgg aaacggcggc 11821 agacgaaaat tgcaattatg atattgcctg gacgggaaag gttgaataca aacctgtcat 11881 gaagctggac aaggattttg acgtggcaat gaagaagctg gagactctta atgtcataaa 11941 cagcggactt cccggactgg attgcggtgc gtgcggtgca ccgagctgca gagcccttgc 12001 ggaagacata gtaaggggga atgccaacga gacggattgc atatttaaac tgagagaaaa 12061 agtaagagac cttgcatttc agatgatgga gctggaagca aaaatgcctc ctgtgctgga 12121 caaggatgaa acttcggaaa acaaatcaaa gaaaagggga gagttttgat gaaggttaag 12181 gagtttgcag agcagttgaa aatggagata ttaaccgggg aaaaaggact tgaaaaagaa 12241 ataaagggta tgtatatatg cgacctttta agttgggtta tgtctcatgc tgccaaagga 12301 gatgcgtgga ttacggtgca tactcatgtc aatattgtgg cagttgctct gatggccgac 12361 ataccttgta ttattattcc cgagggtatt gaagtggagg aggcaactat aaaaaaagct 12421 gtggaagagg acattgcaat attgagaaca tccaaaaccg cctatcaaat agcatgtgaa 12481 gcaggtaaat tattatgata gtgtacacag accttcatat tcattctgca ttatctccgt 12541 gctccgataa tgagatgaca cccaacaata ttgtcaacat ggcctttctc aaacagcttg 12601 acattattgc ggtgaccgat cacaattctg ccgaaaactg tgttgcagtg gtcgagtgtg 12661 ccaaaaagag agggattgtt gcggttcccg gaatggagct tgaaaccagg gaagaggttc 12721 accttgtatg cctttttccg ggtattgatg aggcgcttga catgcagaag atagtatata 12781 atgcactccc ggacataaaa aatcgggaag acatatttgg agagcaaatt gtaatggatg 12841 ccggggataa tgttttaagg catgtggaca gacttctttt gactgcatca aatcttagcg 12901 ttgaagatgt gttcagccat gttgcggcat tgggaggtgt tgttatacct gcccatgtgg 12961 accgagattc ttacagtatt atttcaaatc ttggaatcat tcctgaaaat cctgaaataa 13021 aatatctcga aatctcaaaa ttctgcgaca aaacgaaata tcttgagaaa aacccccatc 13081 ttggaagatt taattttatc agatcttctg atgcccattc attgggcgac attctggaaa 13141 gggaaaattc tattgaactt gaggaaataa gcatagaatg ccttattgag acgttaaaaa 13201 agtgataagg cattgttaga ccatatattg catatgacat ttcattagga tactgtatac 13261 aattcaacgg tgccgttctg acctcttgga tttcccggaa atcttgatga taaaagcatt 13321 tgaaactgtc atcagaacat taaaagtaat actaaaatgt attgaataat gtataaatat 13381 ttgatataat taaggcgata ttgttaaatt gttaacatgc gttttgaaag tgaaatcaaa 13441 cgtctggtta gggggttatt aaagaaatga gcgcaaatgg ttgctgctgt tgcggcggag 13501 tagactccag agaacaaaaa cttaacgaaa taatcgaaaa atacaaaaac acaaaaggag 13561 cgcttgttcc tgttcttcat gaagcacagg aagtatatgg ttatttgcct ctggaggttc 13621 agaaaaaaat tgctgaaggg cttaacatac ctttggctga agtttacggt gttgtaacat 13681 tctataccca gttttccttg aatccaaaag gaaaatataa aatccaggtt tgcatgggta 13741 ctgcatgtta tgtaaaagga tccggagcta ttttggaaaa gcttaaagaa aagcttgaga 13801 ttgatgttgg cgaatgcacc agtgacggca agttttcttt ggaagcttgc agatgtatag 13861 gagcttgtgg attggccccg gtaataatga ttaacgacga tgtatacggc agacttgtac 13921 ctgatgatat agaagggata attgaaaagt ataagaaaga atgagagact tatctttgca 13981 tttgatggat atagttcaaa actcgataga tgctaaagca agcaaaatca gcattttaat 14041 atgtgctgat aagaagtcgg atcttttgga aattgaagta aaagacaatg gtattggaat 14101 ggacagcgat tttctgaaag aggttgtcaa tccttttacc actacaaggg acacaagaag 14161 agtgggtctt ggaattccgc ttttgaaagc gtctgcaaaa agagcttcag gtgatcttga 14221 aattacatct gaaaaactcg tcggtactac ggttaaagca agcttcaaaa tatcccacat 14281 agatcggctt ccgttgggaa atctggctca gaccatggaa agtcttattg ttgcacgacc 14341 tgacattgaa tacgacctta ctcttgacaa caaaaaggaa agatttattt tcaactcctt 14401 tgaaataaaa aggagaatcg gagaagttcc gttaactcat tttgaagtgc taaagtggat 14461 tggcgagtat gtaaatgatg gtgtaaagac tatttttgga gggatattag atgaaatcct 14521 tggctgaatt agaagaaata agaaagagga ctcttgatca aataaatttg aggaccagca 14581 atcatgacat cagagttgtt gtgggtatgg ccacatgtgg tatcgctgcc ggagcaagac 14641 cggttatgaa tgcgtttgtt gaagaattaa acaagaggaa tttgaccaat gtagcggtta 14701 ctatgacagg atgtatcgga atgtgtaaac ttgaacctat tgttgaggta attgacaagg 14761 acggcagcaa ggtaacatat gtaaaaatga ctcctgaaaa ggttgcccgt gttgttgctg 14821 agcacatagt caatggaagg gtatgtactg agtataccat cgcagaggcg gacaaatagt 14881 tagtttcgat ttttgttaag gagggttata aatgcaatta tatagagcac atgttctggt 14941 ttgtggaggt acaggctgta cttcatcaaa ctccaataag ataatcactg aattggaaga 15001 acaaattgcc agaaacggta tacagaatga agtaaaagtt gtaagaaccg gatgctttgg 15061 actttgcgcc gaaggcccga ttatggtggt ttatcctgaa ggtgcaatgt atacaatggt 15121 caaagttgaa gatgtgaaag agatagttga ggagcatctg gtaaaaggaa gaattgtaaa 15181 aagacttctt ccaggcggcg gtggcaagga tgaaaaagct tcccttgaag gcaatgactt 15241 ctttgcaaag cagcaaagaa ttgcgttaag aaactgcggt gttataaatc ctgaaaatat 15301 tgacgaatat attgcttttg acggatataa ggcattggca aaagtattga ctgaaatgac 15361 tcccgaacaa gttgttgatg ttattaaaag atccggtttg agaggaagag ggggcggagg 15421 tttccctacc ggacttaaat gggagtttgc aatgaaacag gatgcagacc agaaatatgt 15481 ttgctgtaat gccgacgagg gagacccggg agcattcatg gacagaagtg tccttgaagg 15541 agaccctcat tctgtaattg aagctatggc gattgcaggt tatgccatag gcgcaaatca 15601 gggatatgtt tatgtaagag cggaataccc gattgccgta aagaggcttg gcattgccat 15661 tcagcaggca agagaatatg gacttttggg taaaaacata tttggaacgg actttagctt 15721 tgatgttgat ataaggcttg gagcaggtgc ttttgtgtgc ggtgaggaaa cagctctcat 15781 gacgtccata gagggacaca ggggagagcc aagaccaagg cctccgttcc ctgcggtaaa 15841 aggtttgtgg caaaagccga ctttgctgaa taacgttgag acttatgcaa acattcccca 15901 gattattctg aaaggtcccg aatggtttgc aagcattggt actgaaaaga gtaaaggtac 15961 caaagtattt gcggtgggcg gtaaaattaa caatacaggt ttggtagaaa ttcctatggg 16021 tacaactttg agagaggtta tttatgacat aggaggagga ataccgaacg gcaagaagtt 16081 caaagcagcc cagaccggag gtccttccgg aggatgtatt cctgcaagcc atattgatac 16141 gccaattgac tatgattcat taacccagtt gggctcaatg atgggttccg gcggacttat 16201 aatcatggat gaagacacat gtatggttga cattgcaaag ttcttccttg aatttactgt 16261 tgacgaatcc tgcggtaagt gtccgccgtg ccgtatagga acaaagagaa tgtatgagat 16321 tcttgaaagg attactgaag gcaagggaga agaaggggac attgaaaagc tggagatgct 16381 tgcaaagaat ataaaggcat cggctttgtg cggactgggt cagacagctc cgaatcctat 16441 tctcagtact ttaagatact tcaggcatga atatattgag cacgtaaggg ataagaagtg 16501 tgccgcaggg gtttgcaagg cgttgatgca ttatgaaata gatgctgaga aatgtaagag 16561 ctgcggaata tgcgcaagac aatgtcctgt aaaagcaata agcggagaaa agaaggttcc 16621 gtacgttata gatcagaaca aatgtatcaa atgtggcgtt tgtatggaga aatgtccgtt 16681 caaggccatt tccaaaaaag cctaatggaa ggggatgtga aaagatgcaa atggtaaatg 16741 ttactataga taattgcaag atacaggtac ctgccaatta taccgtgttg gaagctgcaa 16801 aacaggctaa catagacata cctactcttt gcttcctcaa ggatataaat gaagtaggtg 16861 cctgccgtat gtgcgttgtt gaggtaaaag gtgccagaag cttacaggcg gcctgtgtat 16921 atccggtgtc cgaaggtctt gaggtgtaca ctcagacacc ggcggtaagg gaagccagga 16981 aagtgacttt ggaacttata ctgtcaaacc atgaaaagaa atgtttgacc tgtgtaagaa 17041 gtgaaaactg cgaattgcaa agactggcaa aagatctgaa tgtaaaagat atcagatttg 17101 aaggtgaaat gagcaatttg ccgatagatg atctttcgcc ttctgttgta agggatccca 17161 acaagtgtgt tttgtgcaga cgctgtgtca gcatgtgcaa gaatgttcag accgttggag 17221 ccattgatgt tactgaaaga ggattccgta ccaccgtatc aacggccttt aacaaacctc 17281 tcagtgaagt accctgcgta aactgcggac agtgtatcaa tgtatgtcct gtgggagcat 17341 tgagagaaaa ggacgatatt gacaaggttt gggaagctct tgcaaatcct gagcttcatg 17401 tagtcgttca gacggctcct gcagtcaggg ttgcattggg agaagagttt ggaatgccta 17461 tcggctcaag agtgaccggt aaaatggtgg cagcattgag tcgactgggc tttaaaaagg 17521 tatttgatac agatacggct gccgacctta caataatgga ggaaggtact gagcttataa 17581 acaggattaa aaacggcggc aagcttcctt tgataacttc ctgcagcccg ggatggataa 17641 agttctgcga acacaactat cctgagtttt tagacaatct gtccagctgc aaatcgcctc 17701 acgaaatgtt tggtgcggtt ttgaaatcct actatgcaca gaaaaacgga attgatcctt 17761 caaaagtatt tgttgtatca ataatgccat gtacggcaaa gaagtttgag gctcaaaggc 17821 cggagctttc ttcaacgggt tatcctgatg tggatgttgt tcttaccaca agagagcttg 17881 caagaatgat aaaagaagcg ggtattgatt ttaattccct tccggataaa cagtttgatg 17941 atcctatggg tgaggcatcc ggagcaggtg ttatttttgg tgccaccgga ggagttatgg 18001 aggctgccat caggaccgtc ggtgaattat tgagcggcaa acctgcagac aagattgaat 18061 atactgaggt aagaggtctt gacggtataa aagaggcttc catagaactt gacggtttta 18121 ctctgaaggc tgctgttgcc catggtcttg gcaacgcaag aaagcttctt gacaaaataa 18181 aagccggaga ggcggattat catttcattg aaataatggc ctgtcccggt ggttgtataa 18241 acggtggagg acagcccata cagccgtcat ctgtgagaaa ctggaaagat ataagatgcg 18301 agagggcgaa agctatttac gaagaggatg agtccttgcc tataagaaaa tctcatgaaa 18361 atccaaagat aaagatgctg tatgaagaat tctttggtga accgggcagt cataaagctc 18421 acgagctttt gcacactcat tatgagaaga gggaaaacta ccctgttaaa tgagtttaaa 18481 ataaacagga aaacttaaga aaacgggaaa ctatttcctg ttgagaatgt gaaattaaat 18541 gataaaaagt gatataatag ggtcaggcgt acagaagcct gacctttttg caaaattgtt 18601 ttattggcgc ggcgtatata tacagcatat atacaatgta aatttataca atcaaattgt 18661 tgatacaatc gaattatgcg gctaaaaatt tgctgcaaaa aaattaatat aaaagaggta 18721 ggaaaatggc aaaacaactt tggaaaccgt ctactatatt aaatccggtt ccggttgtaa 18781 tggtaagctg ttgcggcaga gagggaaagc ccaatataat tactgtcgca tgggccggaa 18841 caattaattc aaaacctcct atggtttcaa tatccataag gaaagagcgg tattcctata 18901 atctaataaa ggaaaaagga cagtttgtaa taaatcttac aaccagaaag ctggcatttg 18961 ccgctgattt ttgcggtgta aagtccggca gggatgttga taagtttgaa gttctcaagc 19021 tgacacagga gaaggcctct gtagtggatg taccaatgat aaaggaaagt cctgtgaata 19081 ttgaatgcac ggtgaaggat ataattgaat tgggctctca tgacatgttc atagcacaga 19141 ttgaagccgt aagtgtggat gaatccctta ttgacaagaa agggaggctg tgcatggaga 19201 aagcggatct tatatgctat tcccatggag aatactggtc tttggaaaag acattgggat 19261 attttggata ttcgataacc aaaagaaaag aggttttaaa aaggagatta aataaaaaag 19321 acaggtaaag gagtaaaacc tttgcctgtc ttttttattt tagtatcata tatcttgatt 19381 ttagttttat attcttgcaa ctccggtttt tcttgctgct tcagcaactg ctgcagcaac 19441 tgtttttccg actcttgggt caaatgggag cggaagaatg aagtcaggat tgagctcttc 19501 atcgcttacc agagaagcta ttgcttttgc agcggctatc ttcatttcat cattgatatc 19561 tcttgctctt acatcaagcg cacctctgaa tattccgggg aacgcaagaa cgttgtttat 19621 ctggttcggg aagtcggatc ttccggtacc cactaccttt gctcctgctt cttttgcttc 19681 atcaggcatt atttcaggag taggatttgc catagcaaag ataatcggat cctttgccat 19741 ggattttacc atatcctttg taactgttcc tggaacggaa agaccgatga atacgtcagc 19801 tcccttgatt acatcttcaa gagtaccttt ctttttctcg aggttcgaga ttttagcaat 19861 cagggctttt tcactgttta agttgtctct tccatcataa attgcacctt tggtatcgca 19921 aaggataact ttcttaagcc ccatactcat gagcagtctt gttacagcta tgccggcagc 19981 acctgaaccg tttacaacaa cttctatatc ctcgattttc ttgttgacaa gctttaatgc 20041 attgatcatt gctgcaacag taacaacggc tgtaccgtgc tggtcatcat ggaatattgg 20101 aatgtcacat tcctctttga gtcttctttc tatttcaaag catctcggag cggatatatc 20161 ttcgaggttt ataccgccaa agcttccgga gatgagcttg attgtcttta caatttcatc 20221 tacgtctttt gatttgatac agagcggaaa tgcgtccaca tcaccaaacc tcttgaagag 20281 tacgcattta ccttccataa caggcattcc ggcttcaggt cctatgtctc cgagccctaa 20341 aaccgccgta ccgtcggtaa taaccgctac caggttccaa cgtcttgtat attcataaga 20401 aagattaaca tctttctgaa ttgcaagaca tggttctgca acacccggtg tataagcaag 20461 cgacaactct tccttggttg aaacaggtac cttgtgtata acctcaattt tacccttcca 20521 ctcaccgtga agccttagtg attcttttct gtaatccatt tgattctacc tccaaattat 20581 attattaaat atctgcgata ttaatgcaca attataaatt cttaacttcg ttcaatactt 20641 ttttaacctg ctccgctgag aatcttaaag cttcttcttc ttcaggagtc agattaaatt 20701 ggagaacttc ctgaacacct tcggaattta cgatggatgg aaggcttatt gcaacatctt 20761 ctattccata catgccgttt ataacggttc ctacggttct tattgtattc tgattcttaa 20821 ggagtgtttc aactattgtg ttgattgaaa ctgcaatacc atagtatgtt gcacctttgt 20881 tcttgataat ggttgcaccc gcagttttaa catcttcagc gatttttttc ttgtcttctt 20941 ctgtgaaatt gcatttcgga tcatcgatat attcgttgat atttttaccg gcgatatgtg 21001 tgcagctcca caacggaagc tgtgaatcac cgtgttcgcc tattatgtag ccgtgtacat 21061 tctttacgtc aacgcccaat ttttcgctta acaagtatct gaatctgata ctgtcaagta 21121 cggtacctga acctataact tttcccacag ggaggcctga ccatttttgg atcatataag 21181 ttataatgtc aacaggattg gatactacaa gtattacacc atggttgtaa tacttcatga 21241 tgttttgagt tacttctttt gcaatcataa cgtttttctt tgcaaggtca agacgtgttt 21301 caccaggttt tctgttggct ccggccgtga ctacgataac atcacagtct ttaacgtcgg 21361 aataatcacc ggcatacaat gacatctgtc ccataaatgg aagaccgtgg ttaatatcca 21421 tagcctcgcc tatggctttt tccttgaaaa catcgatgag aacaagttca tttgcggttt 21481 gccgcaaagc cattgtgaag gctgcagacg cacctacaaa accagcacca atgattgcaa 21541 cttttgacct actttttacc atttccatac cattcctttc aattacccag tatatttaac 21601 ggttagtttg tttataaatt tgagattaat tctttaaatt ttaactgtga acccggttca 21661 caggtattat cattaatttc agtatatgtg tttaataaaa attagtgaaa atttgcaact 21721 gcaagcattt aaaattgtaa acgataaata aatcccggca acaaatttcc cccattttaa 21781 atagcccagt taaacacatt gataacattt taacattatt ttatatctgc gtccataact 21841 gaaaaaggga aatccattac tttatgaaat caaattttga agttatcaag aaattatgac 21901 gattttctcc gtggcatgca agatttcgcg atatttcatt cgtttatatt aattttttat 21961 gaaaactgcg gtttgggctg acaattgcga tggaagtctc aattagactt tttgtcaaat 22021 attatgtata ataatattat ctataataat gtatgaaaaa attgtcctaa gatggaagac 22081 gggggtggtt tcatatatgg ttaaatatct gaagaggcag gaagagttgg tagaagaagc 22141 tctctcaaag gataactgtt ctgactggga aagtttgaga aattatcata agtcccaaat 22201 tgaatttttg cagcatgaga gacttgtaca tttattggta acattgtctt ttgggttttt 22261 ctttcttata tccgttcttg ccgccgcggt ttcggaaaaa tttgaaatat tgcttgtttc 22321 ccttcttttg ttggtacttt tgatacctta tattgcccat tattacaaac tggagaacgg 22381 agttcagagg ctttatgagc tttataacaa aattgatgaa aaatgtgtaa ggaaaaacaa 22441 gaccgcctga gttctcaccc agacggtcgg tattggcagt ttcactttcg ttagtcgatg 22501 tttttcatgc cggcaaagaa attattttct tgcaagaacc tttttcagtt ttgcaaatct 22561 tggaagacca tcttcgatag gaggtcttga ttctccctga attaacggaa gagcatactt 22621 aataaagtct tctgtaaggc ctgctccgtc aggtttaatc cattccaacg gaactttctt 22681 ctcagtattt gcaacttcac tgaggttcag aagcttgata ttgcacttgt attcaggacc 22741 ttccgctctt tcaaaagcaa ccatgtagtc tgttttccct tcaacggcat attgtacggc 22801 tgcctgtcct gcaagataag cttcatttac gtcggtaaga gaagctacgt gagctgcgca 22861 tctttggaga aggctgaatt caatgccgcg aacctttgcg ccggtcttct ctttaacaat 22921 gttagccagt gttgaagcaa gaccgcccaa ctgtgcatgt ccaaaggagt cttttgtttt 22981 cgcaaggtct gaaccgtatt cggaaatata ttttccgttt ttgtctttga taccttcaga 23041 tacggctaca ataacctttc cgttttcttt gtagattctt gtcacatctt caacaaattt 23101 gtctatgtca aaggaaagct cgggaaggta tatcaaatca ggaccattgc ccttgtatga 23161 agccaaagct gcggcagcgg taagccagcc tgcgtttctt cccatgattt cgagaattgt 23221 tatcattcct gtatcgtaaa ctcttgcatc atgataaact tccataatgg atgttgctat 23281 atattttgcg gcacttccgt aaccggggca gtggtctgtt ccccaaaggt cgttgtctat 23341 tgtcttcggc acacccatta ctctgcactc atagccaacc ttctgcatgt atttgctgac 23401 cttgttgcag gtgtccattg agtcgtttcc gccattgtag aagaagtatc tgatattgta 23461 tttcttgaat acttccaaaa gtctcttgta atccgtttca tcctcgtctg cgtctttgag 23521 tttgtatctt acagaaccca gagcagatga aggggtagtt tttaaaagtt ccaattcata 23581 aggatcttcc ttactcatgt caaagaagtt ttcttccaaa atacccttga ttccatgagc 23641 tgctccgtaa actgctgtga tacaatcctg ctttaaagct tcctgaataa caccagccgc 23701 acttgcgttg ataactgatg tcggaccgcc ggattgtccg aatatgcatg caccttctaa 23761 acggctcata ttaaaacctc cttaaagtat gtaaaatata tgtgtttctg ctataatgtt 23821 aaattttaaa cacaactaaa taaaaatata acataattga ttttgaaatg caattctgca 23881 taaaccaaaa atatgaatta atttttattt tatccttgat tttcgatttt aatttgataa 23941 aataaatatg ataaaaactt aacaagagtg gctataataa ctaatacact atatttaata 24001 taaggagttg aaaatatgcc attagttacc agtactgaaa tgtttaaaaa agcctacgaa 24061 ggcaaatacg ctataggtgc tttcaatgtt aacaatatgg aaattattca aggtattacc 24121 gaagcggcga aggaagttaa tgcaccgttg attcttcaag tttccgcagg tgcaagaaag 24181 tatgcaaatc atacatacct ggtaaaactt gttgaagccg ctgttgaaga gacgggactt 24241 cctatctgcc tgcatcttga ccatggtgac agcttcgagc tttgtaaatc atgtattgac 24301 ggcggattta catccgttat gattgacggt tcccatcttc cttttgaaga gaacataaaa 24361 cttacaaagc aggttgttga ctacgctcac tcaaaaggtg ttgttgttga aggagagctg 24421 ggaagacttg caggtataga agacgatgtt aatgtgtctg aggctgacgc tgcatttacc 24481 gaccctgacc aggctgaaga gtttgtaaag agaacaggtg ttgattccct ggccatagca 24541 attggtacca gccatggtgc ttacaaattt aagggagaag caaaattaag atttgatata 24601 ttggaagaga ttgaaaagag acttccggga ttcccgattg ttttgcacgg tgcttcctca 24661 gttatacccg aatacgttga tatgattaac aaatatggcg gagatatgcc cggagcgaag 24721 ggtgtaccgg aagacatgct cagaaaggct gcttccatgg ctgtctgcaa gataaacata 24781 gactcagact taagacttgc catgacagcg acaatcagga agtactttgc tgaaaatccg 24841 tcacactttg acccaagaca gtacttaggt cccgcaagaa atgcaattaa agagcttgtt 24901 aaacacaaaa ttgttaatgt tcttggatgc gacggaaaag cttaatattt caaattgtga 24961 tataataaat agtggaggtc ggttgctgac cttcactttt tatgtttact aataaatata 25021 acaaatatgg aacagaaagg catgaataca aatatggaca attttgaaac tgccggcaat 25081 ggaaacagca aaaagcaaaa agttttcaaa gaaattgtca gctggtcatt gtgtttgctg 25141 ggagccttta taatagctct tttgcttaca aagtatgtta ttgtgaatgc gtatgttccc 25201 accggctcaa tggaaaatac gattatgccg ggagacagga ttattgcaag caggatccat 25261 tattattttt ccgagcctaa aagaggagat attgtggtgt tccgatatcc ggacaatgaa 25321 gaggtgcttt atgtaaagag gattatcggg cttccgaatg aaacagtgga aataaaggac 25381 ggaaatgtgt atattaacgg aaagctcctt gaggaaccat atattaaaga aaaggcatac 25441 ggggattttg gaccttatga agttccagaa ggctgttatt ttatgctggg ggacaacaga 25501 aacggttcca cggattcgag aagatggacc aataaatatg ttaaaaaaga aaagattctt 25561 ggaaaagctc tttttaaata ttttcctggg tttaagattt tatggtaaga caaatgtttt 25621 taaaagctcg gatgtcattc tgagctttgt ttatataaaa gcgtttttat aataacattg 25681 aacttaaggt tgaaagtgtg ataatattga attggaattg aattggaatt aaattggaat 25741 tgcaataaat actgcgataa atacattgta aaaaattaat tttgcaaaaa ctaatctttg 25801 agtgaaattt atattgtcag agaatttata ttatcaagct tatattatga taaaaattct 25861 atgtgggggt tgttcaatat ggtacataaa aagaaaacag ctgttataac cggtgtggcg 25921 gttttggttg gtatagtgat tattgctttg gcggtgggat ataattatta ttccaaaaga 25981 aacaagcccc aggagtccaa agagtttggt tttcaggctc ttaaaattaa cggcacttat 26041 gtgagtacag atattatgaa agaagaaagg aataagtttt ttgaaaagta taaaagaaat 26101 gcggatgttt tgagaatgaa tgatcacgaa cgcaatgaca tgcttttgga ccaggtgatt 26161 gaaagacttt tactggaaga ctatgtaaac aacaaatccg gtgtaactgc aacggacagt 26221 gaagtggagg attacataaa caggtttatt aaaccaagat acggagattc cctcggtaca 26281 tttatgagtt cccaggggta cacaaacgaa gaggagatga aagccggtat caaggagtac 26341 atattaaagc acaaagcctt gtacaaagct gcaaaagaga aaaatgtgac tttgaccgag 26401 caggaactgg atgaaggcta cgaaaaacac aagattcaaa acaagaaagt tgacataagg 26461 catatattta tctccagcca ggaaaggggc aaggaggagg ctaaaaagct ggccgacgag 26521 atttataaca gacttaagaa caatgaagat tttgaaactc tggcaaagca atattccgat 26581 gacgagaaaa ctaaagaatc gggaggagtg atcacggaac ttcgagcagg tttcaatgaa 26641 gcggtctttg acaatgcggt ttttacggca gaggccgggc agttgctgga gccgatagag 26701 gttgccaggg gatatgagat tgtatatgtg gacaaggtta cggattttta cagaaccaga 26761 gacgagtatg cggaactcct tacagtggat aaatttatgc agtccgatgc atacaaggaa 26821 tggttcgagg aatacaagaa aaactatgat atagagataa cggatccggc aatgaaggcg 26881 ttcaggcttt tcagagaaaa gcagtataat gaagccggag cactctatga agaactttac 26941 aagtcggaaa aagatgcata ctatattgaa atggcatgtg aggcatacaa acttgccgag 27001 aattgggccg gacttattga agctggcaaa ttggcagtta aagaaaatcc tgatttcgtt 27061 aattattatt tataccaggc agagggagaa tttaaggtcg gagacgccaa taaagctaaa 27121 gagttgttga aagaggcgga gaaaaaggcg ggagacaata catatttcct ggatttgatt 27181 cgaaaaatgt atgagagcca gggccttgcc gaagatgtcg aaagaataga ccggaaattt 27241 caggaaatat ctgagaaatt gaaaggataa acataaattt gaataatcca tgtcctgtaa 27301 gtaaataata agctctgagg tgttgaaaat ggacgtaaaa gtcggaatac ccagagcttt 27361 attttattat cagtattatc ccctctggaa aactttcttt gaggagcttg atgcacaagt 27421 agtattgtcg gaacatacca cagctaaaat cctggatgaa ggtataaagt catgcgtttc 27481 cgaagcttgt ttgcccgtaa agatttttta cggtcatgta atgaatataa aagacaaagt 27541 tgattatttg tttataccaa gatttacaag catatcaaaa aaagaatata tatgcccgaa 27601 gtttggtgga ttgcctgata tgataagaaa cagcataaaa ggtctcccac cgttgattga 27661 tacagagatt aacctaagaa agtccaaaaa aaatgcaata aaagccgcaa tggagacagg 27721 cggttttttt acaaaggata aaagaaaaat agagagagca tataaaaagg ctttggaaag 27781 ctacagggaa tttaatgaga aaataaagca gggagcgctt ccctctgata ttttggacag 27841 aaagctaaat gtaataaaga aaagcaatga gcctgaactt aacattttag tgataggtca 27901 tgtctacaat ctgtatgata gctttatcaa tatggatttg atagacaagc tgagaaaaaa 27961 tggagtaaaa ataatgacca tagatatggt ggaagaggat attgcaagga aaaatgccgg 28021 cctgcttccc aaaaagatgt tctggaattt tggaagcagg gcttttggaa gcgtgatgaa 28081 ttttttggat aaggaagata tagacgggat aatatattta atgagttttg gctgcggagt 28141 ggattctttc gtgtgtgatt tgatagaaag gagagtaaga aaaacaaagg atgttccttt 28201 tataattctg acattggatg aacattccgg ccaggcgggc atggatacaa ggcttgaggc 28261 ttttattgac atggtcagat ggaggtatcg aaatgaaaat aacgtttccg catatgggta 28321 acacatacat tgcagtaaag gctcttttgg acgatatcgg agcagagtat gttattcctc 28381 ctttaaacag caaacgttct ttggaactgg gaacaaagta tgctccggaa atggcatgcc 28441 tgccccttaa aatcaatatt ggaaattata ttgaggctta cgaaaaagga gcggatacaa 28501 ttctgactgc cggcggacgg gggccttgca gatttgggta ctattgtgaa atgtgcaggg 28561 aaatacttaa tgacaacgga tataccatgg atgtgatagt tttggaacct ccggatgtgg 28621 aaattatgaa atttatcggc aaaatcagaa agcttgcagg tggtcttaat atataccata 28681 ttttaaaggt aataaaaaac acaactattg tggcccaaag ggtggatgaa cttgaacgtt 28741 tggcatttaa aatccgcccc agagagctta ggaagggaag tactgacaga atttatgaag 28801 gctttcgcag aaaagtaatt gacgtaaaag gttcgcagga aattctaaag cttgtggagg 28861 ataccaagaa tcagcttttg aaactcgaaa tagacaaaga tgcaaggccg cttaagatcg 28921 ctattgtcgg ggaaatttac acgacaatag actcctatac cagttttaat attgattcga 28981 tattgggcgg catgggtgtt gaggtacaca gggcaaacac catcagcggc tggataatcg 29041 agcatatact gaaagcagtt gttccgttta caaaagacaa aagatatgct gaggctgcaa 29101 agccttatct gggcaccatg ataggagggc atgcccagga aactattggg aatacggtgc 29161 tttatgccaa agacggcttt gacgggataa tacagattta tcctttaacc tgtatgccgg 29221 aaatagtggc agagagtata cttcctgctg tggaaagaga ttataacata cccatactta 29281 ccttgattat tgatgagatg acgggagaag ccgggtatat gacaaggata gaagcttttg 29341 tggatttact gagaaaaaga agggagagag gagaaattgc tgaaaacctc gtattatctg 29401 ggaattgatg tcggttcggt cagcacgaac cttgtggtaa tcaatgaaaa tgatgagata 29461 gttgaaaaat tgtacttaag aacaagcgga cagcctataa atgcactgaa aaatggtatg 29521 aagacactgt accaaaggct tggcaaggac gtgaaaataa gaggtgtggg aactacagga 29581 agcggcaggc agcttgcaag cgtaattgtg ggagctgata ttgtcaaaaa cgagattaca 29641 actcacgcta ttgcggcaca gaaacttgtg cctgaggtaa gaaccataat agaaataggc 29701 gggcaggatt caaagataat tattctgaaa aacggtatta tttatgattt tgccatgaat 29761 accgtttgtg cggcgggaac aggctctttc cttgacaggc aggccgcaag gcttgaaata 29821 ccgattgaag agttcggctc gtttgcacta aggtccaaga ctcctgtaag aattgcagga 29881 cggtgtgctg tatttgcgga atcggatatg atacacaaac agcagaccgg acacagcgtt 29941 gaagatatta tcaacggtct ttgcgaggca ttggtgagga attatctgaa taacctggca 30001 aaaggcaaag acatcgaaga acccatagtc tttcagggcg gagttgccgc gaatgtggga 30061 attgtagctg cttttgaaag agcaatagga aaaaagataa ttatacctca gcactatgat 30121 gtaatgggag cgtacggagc tgcgcttata gcaaaagaag aaatgatgaa aaacggcaaa 30181 aacaccaact tctttggttt tgataatatc cacaatgact ttagagccag aagcatagaa 30241 tgtaacggct gctccaacat gtgcgaagta attgaaatag tatcaaatga tgcggttgtg 30301 gcatgctggg gagaccgatg cggaaaatgg tccgcggtga aaaaggaaaa tcaaagtgtg 30361 gggtgaaaat cggttgaaaa taaaggaatt ttcgtttagt attaaaggca gggcataagt 30421 aaaagaggag gatactatga aagaatattt tgtggacctt catgtgcata tcggaaggtc 30481 ttcaaaagga aaagaggtaa agaaagccac tgccaacaat cttacttttg aaaatatagc 30541 atttgaatcc tacaccagaa aaggcataga tgttattggt gtagttgact gtttgtctcc 30601 atatgtcatt gaagatattg aagagcttgt ggacagagga gagctcatag aaaaacaggg 30661 cgggggaatg gagtatagaa agggacagat attgatattg ggagctgagc ttgaaacaca 30721 tgaagaaaaa ggcggaagct cccattcgct ttgctttttt cccacattaa agtcaatcaa 30781 agactttgcc aaagccatgg agaatcacat aaaaaacatc tataactgtt cctatatgtg 30841 cagattaaca gcccgtcagc ttatagatat agttgacttt tacggcggca cctttattcc 30901 ggcccatgtt ttcactccct ataaaagttt ttatggcaat tgctgcgaca gtctttttga 30961 aatatttgac gagaaatcct ttgataagat tcctgcagtg gaactggggc ttagtgcgga 31021 ttcaatgatg gcatcgcagc ttggcgagct tgacgaaaaa gcctttctca gtaactccga 31081 tgcccactcc ctgtcgaaga tgggcagaga atataatata tttgaaatgg aagaagcaaa 31141 ttacgaagaa atattaaaag cttttaaagg aatcgacgga agaaaaataa aggcaaattt 31201 cggactggat cccaagcttg gaaaatatca caggacttat tgccttgttt gcgactcggt 31261 aataaaagag gatccgccgg tgctaaaatg tcctgtttcc gacaaacacc gtgtggtggt 31321 gggcgtaaag gacagactga tgatgataag agacagggaa aatccgctga tggattcaag 31381 gccgccctat ttttatcagg ttcccttgga gttccttccc aaagtgggcc ctaaaaccat 31441 agacaagctt atagagtttt tcggaagcga gatgagagta ttgcattatg cttcttatga 31501 ggaattgaca aaagtagtga gcgaggatat agcaaaaaat attgtgctgt caagagaagg 31561 aaagctttcc attgaggccg gcggtggtgg agtatacgga aaaatagagg cgtgactgta 31621 agtgtccgtg cagatattat aaaaggggag aagattcaat gcaaatcttt ctcccctttt 31681 tagaaatgaa //