LOCUS NZ_ACVX01000045 21499 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00071, whole genome shotgun sequence. ACCESSION NZ_ACVX01000045 NZ_ACVX00000000 VERSION NZ_ACVX01000045.1 GI:256005857 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000045. FEATURES Location/Qualifiers source 1..21499 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene 17..1183 /locus_tag="ClothDRAFT_2667" CDS 17..1183 /locus_tag="ClothDRAFT_2667" /inference="protein motif:SMART:SM00306" /note="SMART: Hedgehog/intein hint domain protein; KEGG: nme:NMB0655 hypothetical protein" /codon_start=1 /transl_table=11 /product="Hedgehog/intein hint domain protein" /protein_id="ZP_05430806.1" /db_xref="GI:256005858" /db_xref="InterPro:IPR003587" /db_xref="InterPro:IPR006141" /translation="MGVILAGAFWGALAGGLIGGAVGGIAAAINGGSFLEGFADGALS GAISGAVTGAACAGLGALGALAGKGIQCLSTLGKAIKITSKVTAALSFGMDGFDMLAM GISLFDPSNALVEFNRKLHSNALYNRFQIAINALAVFTAGAASTMKCFVAGTLILTAT GFVAIENIKAGDKVIATNPETFEVAEKTVLETYVRETTELLHLTINGEVIKTTFEHPF YVKDVGFVEARKLQIRDRLVDSRGNVLVLEGKKLEITDKPVKVYNFKVDDFYTYHVAH IGVLVHNANNYKPENSIYIYENGIYEDADYHGRVDNKVKSRRPIDGQFALDNSVEVKI GRSPRRVGIDVNGDFVVLDRTDGEKYHGHVRPWKKDASGIEPLSDKMKSALIKA" sig_peptide 17..88 /locus_tag="ClothDRAFT_2667" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.740) with cleavage site probability 0.231 at residue 24" misc_feature 461..865 /locus_tag="ClothDRAFT_2667" /note="Hedgehog/Intein domain, found in Hedgehog proteins as well as proteins which contain inteins and undergo protein splicing (e.g. DnaB, RIR1-2, GyrA and Pol). In protein splicing an intervening polypeptide sequence - the intein - is excised from a...; Region: Hint; cd00081" /db_xref="CDD:73181" misc_feature order(461..463,662..664,860..865) /locus_tag="ClothDRAFT_2667" /note="protein-splicing catalytic site; other site" /db_xref="CDD:73181" misc_feature order(461..463,653..655,662..664) /locus_tag="ClothDRAFT_2667" /note="thioester formation/cholesterol transfer; other site" /db_xref="CDD:73181" misc_feature 479..868 /locus_tag="ClothDRAFT_2667" /note="Protein of unknown function (DUF1557); Region: DUF1557; pfam07591" /db_xref="CDD:148935" gene 1371..1988 /locus_tag="ClothDRAFT_2668" CDS 1371..1988 /locus_tag="ClothDRAFT_2668" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430807.1" /db_xref="GI:256005859" /translation="MMNLVELEISKTQAIKIYLPCQKEDVGSFDIISVKYFREQMEYD LYVNDFAAEAIISLKNMLEEALNFKLQIQNKYLDKGIGYYHNIYYNKLWTTNDSSLTD PGEDFILWSTPTHIGIETFIYNVQNKIYIEICPIYKWNGSCPEDEDENKDEYVPFEVF LSNYKPIDIIVIDKSVAEKWFKLCCEMIEVFEANDKRYLNERKTN" gene 2090..2539 /locus_tag="ClothDRAFT_2669" CDS 2090..2539 /locus_tag="ClothDRAFT_2669" /inference="similar to AA sequence:KEGG:Dtpsy_3336" /note="KEGG: dia:Dtpsy_3336 transposase mutator type" /codon_start=1 /transl_table=11 /product="transposase mutator type" /protein_id="ZP_05430808.1" /db_xref="GI:256005860" /translation="MSTLSKEQVKEIIKGNNFQSVADVSAYLKDIFKDIIQELLEAEL EAKLGYAKDDVENKNTDNSRNGYSTKTIKSEFGEVEIQVPGDRKGEFKPQIIPKYQRD VSGIEEKVIALYARGISTRDISEQIEELYGFSLSPEMVSRITDRIAP" misc_feature 2096..>2536 /locus_tag="ClothDRAFT_2669" /note="Transposase, Mutator family; Region: Transposase_mut; pfam00872" /db_xref="CDD:109910" gene 2592..3308 /locus_tag="ClothDRAFT_2670" CDS 2592..3308 /locus_tag="ClothDRAFT_2670" /inference="protein motif:PFAM:PF00872" /note="PFAM: transposase mutator type; KEGG: sbn:Sbal195_4677 transposase mutator type" /codon_start=1 /transl_table=11 /product="transposase mutator type" /protein_id="ZP_05430809.1" /db_xref="GI:256005861" /db_xref="InterPro:IPR001207" /translation="MDAIHYKVRDEGRIVNRAAYVVIGITVEGSKDILGIWIGDNESS KFWLGVLNDLKNRGVEDVLIFCVDGLTGIKEAINAAYPKAEVQRCIIHQLRNSFKYVS YKDIKAFSKDFKEVYRAINEEVAMEKFCEIKDKWGKEYPYAIRSWENNWDVISPFYKF PEEIRKIIYTTNIIEGLHRQFRKVTKTKTMFPNDSSLEKMLYLGSMNVVKKWTQRYKN WDKVLSQLMIEYPGRLEKYI" misc_feature <2595..3236 /locus_tag="ClothDRAFT_2670" /note="Transposase, Mutator family; Region: Transposase_mut; pfam00872" /db_xref="CDD:109910" misc_feature 2640..2879 /locus_tag="ClothDRAFT_2670" /note="MULE transposase domain; Region: MULE; pfam10551" /db_xref="CDD:151094" gene 4237..5865 /locus_tag="ClothDRAFT_2671" CDS 4237..5865 /locus_tag="ClothDRAFT_2671" /EC_number="6.3.4.2" /inference="protein motif:TFAM:TIGR00337" /note="KEGG: dat:HRM2_26840 PyrG; TIGRFAM: CTP synthase; PFAM: CTP synthase-like; glutamine amidotransferase class-I" /codon_start=1 /transl_table=11 /product="CTP synthase" /protein_id="ZP_05430810.1" /db_xref="GI:256005862" /db_xref="InterPro:IPR000991" /db_xref="InterPro:IPR004468" /db_xref="InterPro:IPR017456" /translation="MSVKYIFVTGGVVSGLGKGITAASLGRLLKARGLRVTIQKFDPY INVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSKNNNVTTGKIYWSIISK ERKGDFLGGTVQVIPHVTNEIKNRIYLVGKEGKTDVVITEIGGTVGDIESLPFLEAIR QVASDVGRENVVYIHVTLVPYLSKSGELKTKPTQHSVKELRSLGIQPDIIVCRTEKRL SKELKDKIGLFCNIPGEWVIQNLDAESLYEIPLMLEEEGLANIVCERLNLGCVKPDMT EWCELVNRQKNLSKSLTIALVGKYVELHDAYLSVVESLNHGGIYNDAEVKIKWVNSEE LTEDNLEETLCDVDGILVPGGFGDRGIEGKILAAKYARENKVPYFGICLGMQMAVVEF ARNVAGLKMANSSEFDANSPHPVIDLMPEQKDIDEKGGTMRLGLYPCKIIKDSSAYRI YESELIYERHRHRYEFNNEYRELLTSKGLILAGLSPSEKLVEIIELKDHPWFIGVQFH PEFKSRPNRPHPLFKDFIRAAVERRYKQQENKND" misc_feature 4246..5844 /locus_tag="ClothDRAFT_2671" /note="CTP synthetase; Validated; Region: pyrG; PRK05380" /db_xref="CDD:180047" misc_feature 4246..5010 /locus_tag="ClothDRAFT_2671" /note="CTP synthetase (CTPs) is a two-domain protein, which consists of an N-terminal synthetase domain and C-terminal glutaminase domain. The enzymes hydrolyze the amide bond of glutamine to ammonia and glutamate at the glutaminase domains and transfer...; Region: CTGs; cd03113" /db_xref="CDD:48377" misc_feature order(4276..4278,4285..4296,4354..4356,4360..4368, 4444..4446,4450..4452,4459..4464,4660..4662,4666..4668) /locus_tag="ClothDRAFT_2671" /note="Catalytic site; other site" /db_xref="CDD:48377" misc_feature order(4279..4293,4354..4356,4669..4671,4801..4809, 4909..4911) /locus_tag="ClothDRAFT_2671" /note="active site" /db_xref="CDD:48377" misc_feature order(4354..4368,4666..4671,4687..4689,4801..4809, 4909..4911) /locus_tag="ClothDRAFT_2671" /note="UTP binding site; other site" /db_xref="CDD:48377" misc_feature 5110..5814 /locus_tag="ClothDRAFT_2671" /note="Type 1 glutamine amidotransferase (GATase1) domain found in Cytidine Triphosphate Synthetase; Region: GATase1_CTP_Synthase; cd01746" /db_xref="CDD:153217" misc_feature order(5293..5307,5380..5385,5392..5394,5452..5454, 5617..5628,5758..5760,5764..5766) /locus_tag="ClothDRAFT_2671" /note="active site" /db_xref="CDD:153217" misc_feature order(5299..5301,5383..5385) /locus_tag="ClothDRAFT_2671" /note="putative oxyanion hole; other site" /db_xref="CDD:153217" misc_feature order(5380..5382,5758..5760,5764..5766) /locus_tag="ClothDRAFT_2671" /note="catalytic triad; other site" /db_xref="CDD:153217" gene complement(5985..6953) /locus_tag="ClothDRAFT_2672" CDS complement(5985..6953) /locus_tag="ClothDRAFT_2672" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: swd:Swoo_4720 XRE family transcriptional regulator" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430811.1" /db_xref="GI:256005863" /translation="METIKNYLDNIFANLPKTKEMERLKSEIYYNMEEKYNELKKSGK PENEAIGIVISEFGNIDELLEEMGISPSSNSENHPVIENEEAMEFISLKEKTSYMIAT GVGLILLGVSLLIFLATLVERNLIFKFLPQNAREALPVIVLFLFIIPAVILFMYTENK LQKFKFIEEGKFEISPGLKNILNSELPSVTQKQNIPIITGVSLCILSVVVLLVFGIFE SFAAFGVSIMLLMIAAAVFIFITSTSTAEAYKKLMKIDEYSPERVRQNKVIGAVAGVI WPIATALFLIGGFVFKLWNICWIVFPITGILFGGFAAFYKAIKTDK" gene complement(6940..7284) /locus_tag="ClothDRAFT_2673" CDS complement(6940..7284) /locus_tag="ClothDRAFT_2673" /inference="protein motif:PFAM:PF03551" /note="PFAM: transcriptional regulator PadR family protein; KEGG: rle:RL3102 PadR family transcriptional regulator" /codon_start=1 /transl_table=11 /product="transcriptional regulator, PadR-like family" /protein_id="ZP_05430812.1" /db_xref="GI:256005864" /db_xref="InterPro:IPR005149" /translation="MISSDVIRGYVDTIILSLLIEGDSYGYEISKNIRIKTDELYVIK ETTLYSAFARLEKNGYIKSYYGEETQGKRRTYYRITPEGIKYYKQKCEEWELTKKVIN KFVKELESNGDN" misc_feature complement(7015..7245) /locus_tag="ClothDRAFT_2673" /note="Winged helix-turn-helix (WHTH) DNA-binding domain of the GntR family of transcriptional regulators; Region: WHTH_GntR; cl00088" /db_xref="CDD:185792" gene 7607..8305 /locus_tag="ClothDRAFT_2674" CDS 7607..8305 /locus_tag="ClothDRAFT_2674" /inference="protein motif:TFAM:TIGR02837" /note="TIGRFAM: stage II sporulation protein R" /codon_start=1 /transl_table=11 /product="stage II sporulation protein R" /protein_id="ZP_05430813.1" /db_xref="GI:256005865" /db_xref="InterPro:IPR014202" /translation="MLNSSNNTGKKNIFVKVLTAIILFVSVLSCVGLISYSENVSSGL ADNIIRLHVLANSDSPEDQQLKRDVRDVVINFMKDKLKNVKDVETTKRIIEENSEAIK QVALSEIRRQNKDYDVTVMFGKYPFPTKVYGDISLPTGNYQALRVVIGNGEGQNWWCV LFPPLCFVDATHGTVPDSVKDDLKEVLSEEEYYLITAQDEGDDIEIEIKFKIVEMFQK SKIKIASAMNKIFQ" sig_peptide 7607..7720 /locus_tag="ClothDRAFT_2674" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.966) with cleavage site probability 0.653 at residue 38" misc_feature 7736..8131 /locus_tag="ClothDRAFT_2674" /note="Stage II sporulation protein R (spore_II_R); Region: Spore_II_R; cl09775" /db_xref="CDD:158692" gene 8612..9310 /locus_tag="ClothDRAFT_2675" CDS 8612..9310 /locus_tag="ClothDRAFT_2675" /inference="protein motif:PFAM:PF02308" /note="PFAM: MgtC/SapB transporter; amino acid-binding ACT domain protein; KEGG: gbm:Gbem_2314 MgtC/SapB transporter" /codon_start=1 /transl_table=11 /product="MgtC/SapB transporter" /protein_id="ZP_05430814.1" /db_xref="GI:256005866" /db_xref="InterPro:IPR002912" /db_xref="InterPro:IPR003416" /translation="MINMIRTFFENGGFYLIVIMRLLLACILGGLIGYERESTNRPAG FRTHILVCVGSALVMITSQYIFESYKGITNLDPARLGAQVISGIGFLGAGTIIREGAN VRGLTTAASLWAVSCVGIAAGIGFYEGAVISTIVIYITLILLKRTEKHIARKNNLSIF YIQTEDLPGQIGTIGCIFGKHNITIRNIEFINEKKDEEVLIKFAVKMPSDMSKEKIMD ELQRVNGVKKVTGQ" misc_feature 8714..9070 /locus_tag="ClothDRAFT_2675" /note="MgtC family; Region: MgtC; cl12207" /db_xref="CDD:187193" misc_feature <9086..9301 /locus_tag="ClothDRAFT_2675" /note="D-3-phosphoglycerate dehydrogenase; Region: PGDH; TIGR01327" /db_xref="CDD:162302" gene 9443..10903 /locus_tag="ClothDRAFT_2676" CDS 9443..10903 /locus_tag="ClothDRAFT_2676" /inference="protein motif:PFAM:PF01276" /note="PFAM: Orn/Lys/Arg decarboxylase major region; Orn/Lys/Arg decarboxylase domain protein; aminotransferase class V; aromatic amino acid beta-eliminating lyase/threonine aldolase; KEGG: Os03g0848100; hypothetical protein" /codon_start=1 /transl_table=11 /product="Orn/Lys/Arg decarboxylase major region" /protein_id="ZP_05430815.1" /db_xref="GI:256005867" /db_xref="InterPro:IPR000192" /db_xref="InterPro:IPR000310" /db_xref="InterPro:IPR001597" /db_xref="InterPro:IPR008286" /translation="MVLRMNQLKTPVFDAVKRYVESNVIQFHVPGHKQGAGLEELREY IGETALKMDANGMEDLDFANNPTGVIYESEMLAAQAFGAQHAYFLVNGTTSGVQAMIM SACEPGDKIILPRNAHKSTIGGIILSGAVPVYVQPEINEKLGIAMGITVESLKKAIKE NPHAKAVFIINPTYYGIASDLKSLVRIAHRHEMAVLVDEAHGAHMSFHDDFPLTAMEV GADMSAVSTHKTGGSLTQSSLLLLRGNMISPERVKQVLNLTYTSSASYLLMCSLDIAR KQLATKGSDMLEETLRLARMAREEINKIEGLYAFGKELIGTPGCHDFDETKLGICVSG LGYTGYEMEAKLRKEYNIQIEMSDLSNILAIVSIGDREENLVALINALKDIAAKTEKK EYPKPPIIPPTPKMIVSPRDAFYSPKKIVPLDKSVGEISGEMVMAYPPGIPVVCMGER ITQDIVDYIKILKEQKTQLQGTADPYIDHIMVLGVD" misc_feature 9452..10888 /locus_tag="ClothDRAFT_2676" /note="Arginine/lysine/ornithine decarboxylases [Amino acid transport and metabolism]; Region: LdcC; COG1982" /db_xref="CDD:32165" misc_feature 9473..10351 /locus_tag="ClothDRAFT_2676" /note="Ornithine decarboxylase family. This family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The major groups in this CD corresponds to ornithine decarboxylase (ODC), arginine decarboxylase (ADC) and...; Region: Orn_deC_like; cd00615" /db_xref="CDD:99739" misc_feature order(9599..9601,9611..9613,9635..9637,9710..9718, 9722..9727,9734..9736,9746..9754,9758..9763,9794..9799, 10124..10129,10136..10150,10202..10207,10211..10219, 10223..10225,10244..10246,10253..10255,10262..10264, 10298..10303) /locus_tag="ClothDRAFT_2676" /note="homodimer interface; other site" /db_xref="CDD:99739" misc_feature order(9716..9724,9794..9796,9800..9802,9950..9952, 10034..10036,10040..10045,10118..10120,10124..10129) /locus_tag="ClothDRAFT_2676" /note="pyridoxal 5'-phosphate binding site; other site" /db_xref="CDD:99739" misc_feature 10127..10129 /locus_tag="ClothDRAFT_2676" /note="catalytic residue; other site" /db_xref="CDD:99739" misc_feature <10658..10834 /locus_tag="ClothDRAFT_2676" /note="Orn/Lys/Arg decarboxylase, C-terminal domain; Region: OKR_DC_1_C; pfam03711" /db_xref="CDD:112521" gene 11056..13773 /locus_tag="ClothDRAFT_2677" CDS 11056..13773 /locus_tag="ClothDRAFT_2677" /inference="protein motif:TFAM:TIGR01494" /note="TIGRFAM: ATPase, P-type (transporting), HAD superfamily, subfamily IC; PFAM: E1-E2 ATPase-associated domain protein; cation transporting ATPase domain protein; Haloacid dehalogenase domain protein hydrolase; KEGG: sat:SYN_01009 calcium-transporting ATPase" /codon_start=1 /transl_table=11 /product="ATPase, P-type (transporting), HAD superfamily, subfamily IC" /protein_id="ZP_05430816.1" /db_xref="GI:256005868" /db_xref="InterPro:IPR000695" /db_xref="InterPro:IPR001757" /db_xref="InterPro:IPR004014" /db_xref="InterPro:IPR005834" /db_xref="InterPro:IPR006068" /db_xref="InterPro:IPR008250" /translation="MNILESALQNSNKDNKVKYGLSEKEAEKRYKKYGPNTLAEKKKI SPFKILIAQFSDFMVLILLASTLISAFMGELTEAITIVSIVILNALMGFVQEYRTEKT LEALKSLAAPVAKVVRDGKTVEIPAEKVVPGDLVVLETGDRIPADAVLIESHGLCVDE SLLTGESVPVEKQVEAGGRKNVNAGERNGTVYMGTVVTSGRGKAVVCSTGMMTEMGKI ADMIQNIETEQTPLQKRLDTLGKYIVYGCLTICAIVTVTGILRGEKLFTMFLSGISLA VAAVPEGLPAIVTIALALGVQRMYKRNAFIRKLPAVETLGCASVICSDKTGTLTENKM TVRKIYTNGGFVEVKGSPLSSEGEFISLKRKIDPKNNNSLRLTLEIGVLCNNASMVRI KENRSFGKIASLVPRTEKWELSGDPTEAALLVAGAKGGCTQELLNESYTRIDEIPFDS DRKCMSVICENERGEVFVFTKGAADVIIEKCSKIYTSRGIVDLDEAGVKRVLKANDDM AKEALRVLGVAFRRLDSKNYRLDDVEKDLVFAGLIGMIDPPRKEALDAVRKCKLAGIK PVMITGDHKITAAAIARELNIASEGDRVLTGAQLEQMDDKRLEELVDEVSVYARVSPR HKLRIVRALKKRGHVVAMTGDGVNDAPAIKEADIGVAMGITGTDVTKEASSMILLDDN FATIVAAVEEGRVIYNNIRKFIRYMLSCNIGEVLTMFVGTLIGLPLPLLPIQILWVNL VTDGLPAIALGLEPGDKDIMMRPPRGAKENIFSNGLWQLIIFRGALIGLSTLAVFASI LNFTSNVDVARTAAFVTLVITQLIHVFECKSERKNIFEIPLFNNIPLVLAVLCSLVMI LGVVYIPLFQGIFKTVPLSLNDWILIGGFSALGPVISSFFKVNYKYIRR" misc_feature 11095..13770 /locus_tag="ClothDRAFT_2677" /note="Cation transport ATPase [Inorganic ion transport and metabolism]; Region: MgtA; COG0474" /db_xref="CDD:30822" misc_feature 11113..11277 /locus_tag="ClothDRAFT_2677" /note="Cation transporter/ATPase, N-terminus; Region: Cation_ATPase_N; cl02930" /db_xref="CDD:155184" misc_feature 11296..11997 /locus_tag="ClothDRAFT_2677" /note="E1-E2 ATPase; Region: E1-E2_ATPase; pfam00122" /db_xref="CDD:143896" misc_feature <12667..13038 /locus_tag="ClothDRAFT_2677" /note="Haloacid dehalogenase-like hydrolases. The haloacid dehalogenase-like (HAD) superfamily includes L-2-haloacid dehalogenase, epoxide hydrolase, phosphoserine phosphatase, phosphomannomutase, phosphoglycolate phosphatase, P-type ATPase, and many others...; Region: HAD_like; cl11391" /db_xref="CDD:187016" misc_feature 13264..13749 /locus_tag="ClothDRAFT_2677" /note="Cation transporting ATPase, C-terminus; Region: Cation_ATPase_C; pfam00689" /db_xref="CDD:144331" gene 15226..15912 /locus_tag="ClothDRAFT_2678" CDS 15226..15912 /locus_tag="ClothDRAFT_2678" /inference="protein motif:PFAM:PF00072" /note="PFAM: response regulator receiver; transcriptional regulator domain protein; SMART: response regulator receiver; KEGG: pmr:PMI2891 osmolarity transcriptional regulator (two-component system response regulator)" /codon_start=1 /transl_table=11 /product="two component transcriptional regulator, winged helix family" /protein_id="ZP_05430817.1" /db_xref="GI:256005869" /db_xref="InterPro:IPR001789" /db_xref="InterPro:IPR001867" /translation="MGAKILIVEDENDIGSFMVRTLNRKGFNAIRAETGKEALRLVEL EKPEVILLDIMLPDIDGFEICKRVSRQHPEISIIMVTARGEDVDKIRGLELGADDYII KPFNPMELIARIKAVIRRTNKPDNSNEVVIGPLRIDYNCRQVYKNGVVLDLTPREFCL LKVFVNNLNKALTRDEILNWVWGEDYIGDTKTVDVHVRRLREKIEDDPSHPKYIETVW GKGYLFRKGD" misc_feature 15235..15900 /locus_tag="ClothDRAFT_2678" /note="Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Signal transduction mechanisms / Transcription]; Region: OmpR; COG0745" /db_xref="CDD:31088" misc_feature 15241..15579 /locus_tag="ClothDRAFT_2678" /note="Signal receiver domain; originally thought to be unique to bacteria (CheY, OmpR, NtrC, and PhoB), now recently identified in eukaroytes ETR1 Arabidopsis thaliana; this domain receives the signal from the sensor partner in a two-component systems...; Region: REC; cd00156" /db_xref="CDD:29071" misc_feature order(15250..15255,15382..15384,15406..15408,15466..15468, 15523..15525,15532..15537) /locus_tag="ClothDRAFT_2678" /note="active site" /db_xref="CDD:29071" misc_feature 15382..15384 /locus_tag="ClothDRAFT_2678" /note="phosphorylation site" /db_xref="CDD:29071" misc_feature order(15391..15396,15400..15408) /locus_tag="ClothDRAFT_2678" /note="intermolecular recognition site; other site" /db_xref="CDD:29071" misc_feature 15532..15540 /locus_tag="ClothDRAFT_2678" /note="dimerization interface; other site" /db_xref="CDD:29071" misc_feature 15613..15897 /locus_tag="ClothDRAFT_2678" /note="Effector domain of response regulator. Bacteria and certain eukaryotes like protozoa and higher plants use two-component signal transduction systems to detect and respond to changes in the environment. The system consists of a sensor histidine kinase...; Region: trans_reg_C; cd00383" /db_xref="CDD:29475" misc_feature order(15685..15687,15742..15747,15799..15801,15808..15810, 15832..15837,15871..15873,15886..15888) /locus_tag="ClothDRAFT_2678" /note="DNA binding site" /db_xref="CDD:29475" gene 15914..17305 /locus_tag="ClothDRAFT_2679" CDS 15914..17305 /locus_tag="ClothDRAFT_2679" /inference="protein motif:PFAM:PF02518" /note="PFAM: ATP-binding region ATPase domain protein; histidine kinase HAMP region domain protein; histidine kinase A domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase A domain protein; histidine kinase HAMP region domain protein; KEGG: pmr:PMI1588 two-component system sensor kinase" /codon_start=1 /transl_table=11 /product="histidine kinase" /protein_id="ZP_05430818.1" /db_xref="GI:256005870" /db_xref="InterPro:IPR003594" /db_xref="InterPro:IPR003660" /db_xref="InterPro:IPR003661" /db_xref="InterPro:IPR004358" /db_xref="InterPro:IPR005467" /translation="MRGIRGRVVGTYLIITFLSVVIFEIILIYGLVEFYYSSIEAELS RSAQSIIHNYSQYFNNYDLHRDAKVLLESMPGNTVAQVQIIDDKGVLIADSIEPSMEG KKLDNYDVNMALNGKEAAWKGKIPMTGEPVFSVSFPIIRPDSEEVIGIVRVITTLSDV NEILKNHIIILISLGLCIVFLIFLTGLALTNTIIKPVKEITSAAKAMAQGRFDVRVSK RYDDEIGELGDTLNYMAQEVANHQKMKNDFIASISHELRTPLTSIMGWIITINSGDID SKEELKEGLDIIERESKRLAELVDELLDFSKFDAGIITLRKSVVNLGELLKYIKRQME PRAERKGITMTIDVDEHLPLIEADENRLKQVFINIIDNSFKFTQKGGYIDIIGRKNEN GVLIRIEDSGCGIPEEDLPRVKQRFFKGSNVVSGSGLGLAICDEIVRLHNGKIDIEST VGKGTRVDVILPV" misc_feature 16307..17302 /locus_tag="ClothDRAFT_2679" /note="Signal transduction histidine kinase [Signal transduction mechanisms]; Region: BaeS; COG0642" /db_xref="CDD:30987" misc_feature 16490..16624 /locus_tag="ClothDRAFT_2679" /note="Methyl-accepting protein, and Phosphatase (HAMP) domain. HAMP is a signaling domain which occurs in a wide variety of signaling proteins, many of which are bacterial. The HAMP domain consists of two alpha helices connected by an extended linker. The...; Region: HAMP; cd06225" /db_xref="CDD:100122" misc_feature order(16490..16495,16502..16507,16511..16516,16523..16528, 16532..16534,16580..16585,16589..16594,16601..16606, 16610..16615,16622..16624) /locus_tag="ClothDRAFT_2679" /note="dimerization interface; other site" /db_xref="CDD:100122" misc_feature 16637..16834 /locus_tag="ClothDRAFT_2679" /note="Histidine Kinase A (dimerization/phosphoacceptor) domain; Histidine Kinase A dimers are formed through parallel association of 2 domains creating 4-helix bundles; usually these domains contain a conserved His residue and are activated via trans-...; Region: HisKA; cd00082" /db_xref="CDD:119399" misc_feature order(16655..16657,16667..16669,16679..16681,16688..16690, 16700..16702,16709..16711,16763..16765,16775..16777, 16784..16786,16796..16798,16805..16807,16817..16819) /locus_tag="ClothDRAFT_2679" /note="dimer interface; other site" /db_xref="CDD:119399" misc_feature 16673..16675 /locus_tag="ClothDRAFT_2679" /note="phosphorylation site" /db_xref="CDD:119399" misc_feature 16997..17296 /locus_tag="ClothDRAFT_2679" /note="Histidine kinase-like ATPases; This family includes several ATP-binding proteins for example: histidine kinase, DNA gyrase B, topoisomerases, heat shock protein HSP90, phytochrome-like ATPases and DNA mismatch repair proteins; Region: HATPase_c; cd00075" /db_xref="CDD:28956" misc_feature order(17015..17017,17027..17029,17036..17038,17105..17107, 17111..17113,17117..17119,17123..17128,17195..17206, 17252..17254,17258..17260,17273..17278,17282..17284) /locus_tag="ClothDRAFT_2679" /note="ATP binding site; other site" /db_xref="CDD:28956" misc_feature 17027..17029 /locus_tag="ClothDRAFT_2679" /note="Mg2+ binding site; other site" /db_xref="CDD:28956" misc_feature order(17117..17119,17123..17125,17195..17197,17201..17203) /locus_tag="ClothDRAFT_2679" /note="G-X-G motif; other site" /db_xref="CDD:28956" gene 17406..18686 /locus_tag="ClothDRAFT_2680" CDS 17406..18686 /locus_tag="ClothDRAFT_2680" /inference="protein motif:PFAM:PF00515" /note="PFAM: TPR repeat-containing protein; SMART: Tetratricopeptide domain protein; KEGG: hypothetical protein" /codon_start=1 /transl_table=11 /product="TPR repeat-containing protein" /protein_id="ZP_05430819.1" /db_xref="GI:256005871" /db_xref="InterPro:IPR001440" /db_xref="InterPro:IPR013026" /translation="MGEIRNYIKLILIGVAILVTVTMIISATGCDILPPPASAIKPPQ YINASSGEVEDPKAIARRFLTPGTYFSYPTHPRNADAVWEVDIDGDGRAELLTLYRGR DLKNNGVEVFGVFILKQDENHEWQIFWEQQYTANIVALDWADVRDITGDNYPELLIGW NIGYRIGSNLDIYSLKEEDKPRLISSIKYSEIVGIEDRRGINNEIKPVLMMYLFDEEM YESENEGRKDEYFHILQWDYKEWMGGGLVEADDFYASYYVNVIEDLKESLERYSNNYY QWYILALAQLRSGKPMDALESVGKVLEITQRYINRHGERTVKEVFKYQKKARILKAAA YIKMGKYHESIIESNYVLGNYDIDDKELAQIYLNLGYAYAGLKQYEMAGTYFNKSCET AETVYKEGTALYLLNSYKAKKQLEYIKPFLTSSH" sig_peptide 17406..17495 /locus_tag="ClothDRAFT_2680" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.624) with cleavage site probability 0.565 at residue 30" misc_feature <18192..>18578 /locus_tag="ClothDRAFT_2680" /note="putative PEP-CTERM system TPR-repeat lipoprotein; Region: PEP_TPR_lipo; TIGR02917" /db_xref="CDD:163072" gene 18791..19588 /locus_tag="ClothDRAFT_2681" CDS 18791..19588 /locus_tag="ClothDRAFT_2681" /inference="similar to AA sequence:KEGG:Gbem_0198" /note="KEGG: gbm:Gbem_0198 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430820.1" /db_xref="GI:256005872" /translation="MNLLDRLERKFGKFAIKRLPMYIVILNLFVYMLNLLDSRYINYL YLIPSRVLKGEIWRLVTYIFIPPMTSMEDVLWLFFAIYFYYIMATGLENEWGSFKFNL YYLLGMIGTTIAAFFIPLPATANFINLSIFLAFAYLYPDYQIYLFFIIPIKIKYLGYI SWILIIYELLTVPLIMKFYIIIPILNFFIFFGKDIFVRYKTRGKSVYRRSKYMGTFRE KSYFHKCTVCGVTEKDNPDMEFRYCMTCEGHYEYCMEHLKNHEHIKK" misc_feature 18791..>19270 /locus_tag="ClothDRAFT_2681" /note="Rhomboid family; Region: Rhomboid; cl11446" /db_xref="CDD:187043" gene 19806..21413 /locus_tag="ClothDRAFT_2682" CDS 19806..21413 /locus_tag="ClothDRAFT_2682" /inference="protein motif:PFAM:PF07833" /note="PFAM: copper amine oxidase domain protein; KEGG: hypothetical protein" /codon_start=1 /transl_table=11 /product="copper amine oxidase domain protein" /protein_id="ZP_05430821.1" /db_xref="GI:256005873" /db_xref="InterPro:IPR012854" /translation="MKKIARKISMLLVVALLAVSMVACTSDEIALIEAMSKTSEISSY EGNSKIQLSFKGQGFSEKTQKVFDFLASYVDGFTFEANQKYSSNDEKTKATLAMDGNV DMQGLSVKYKYWTDMDFTTENPSLIQIVELPPAITQPMFTFANTGTKKYITIDYGSVL SAENNGGISLNPENLAKNSVELQEMLLDFVKTTAKDFDPGMVAVTKKGSAVTDKGEKV TEYELKLDDAAAKKLLHAFINDVILQEDTIEFGKKYMEAAINMYDFPEEEKQEALDEI NKGLDEFASQLPAYRDSVTQVFESIKDVKFFGDKGLVAKYYINNDGFLVGGKSSIDIK IKMADFAALLGDNFDEKDKNGVLYLTIDAESSVYNINKEVSIELPEITEENSFDVLKG FMPILSGIGSAPGIGEEDYTYDIPALSDGINVVMNGKVVYFPDVKPENVNGRVLVPIR TISEEMGAEVTYNDATKQVLIAKDDTEILLTIGSQEAYVNGEKIMLDVPAMIIEGRTM VPLRFISESMNATVEWDGEAQIVYIFY" sig_peptide 19806..19883 /locus_tag="ClothDRAFT_2682" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.600 at residue 26" misc_feature 21126..21404 /locus_tag="ClothDRAFT_2682" /note="Copper amine oxidase N-terminal domain; Region: Cu_amine_oxidN1; pfam07833" /db_xref="CDD:149091" ORIGIN 1 tttgacagga ggagtattgg gagtcatact ggcgggagca ttctggggag cattggccgg 61 aggattgata ggaggagcgg ttggaggaat agccgctgcg ataaatggag gttcgttttt 121 ggaaggattt gcggacgggg cattaagcgg agcaatttcc ggagcggtga caggagccgc 181 atgtgccggg ctgggtgctt tgggagctct agcagggaaa ggcatccaat gcttgagcac 241 actggggaaa gcgataaaaa ttacgtcaaa agtaactgca gcactatcgt ttggtatgga 301 tggatttgac atgctggcaa tgggaatatc gttgtttgat ccatccaacg cattggttga 361 atttaaccgg aagctgcatt ccaacgcgct ttacaacaga ttccagattg ctataaacgc 421 gcttgctgtt ttcactgcgg gagcggcatc gacaatgaag tgctttgttg caggtacgct 481 gatattgact gcgacaggct tcgttgcgat agagaatatc aaggcaggag acaaggtaat 541 tgcgacgaat ccggagactt ttgaagtagc ggaaaagaca gtgcttgaga catatgtgag 601 agagacgacg gagcttttgc atttgacaat caatggagag gtaatcaaga caacctttga 661 gcatccgttt tatgttaaag atgtgggttt tgttgaagcg agaaaactgc agataagaga 721 caggttggtt gattcaagag gtaatgtttt agtattggaa ggtaaaaagc ttgaaataac 781 agataagcct gtaaaggttt acaacttcaa agtggatgat ttttatactt atcatgttgc 841 acatattggt gtattggtgc ataatgcaaa caactataaa cctgaaaata gtatatatat 901 ttatgaaaat ggaatatacg aggatgcaga ttatcatgga agagttgata ataaagtaaa 961 aagtagaaga ccaatagacg gtcaatttgc gttggataat tctgttgagg ttaaaattgg 1021 tagaagtcct agaagggttg ggattgatgt aaatggagat tttgttgtat tagatcgtac 1081 ggatggcgaa aaataccatg gtcatgttag accttggaag aaagatgctt caggtattga 1141 acctttaagt gataaaatga aaagtgctct gataaaggca tgatatgtta aagaggtggc 1201 taaaaagcct aaattaacta gtaaggcaat tgatataata aaaaagtatc agcaaaatca 1261 aatttattaa atatattttg ctattaagtg ataattttgt aaaataatga ccaaaaaaat 1321 atctgattag tgattagtta aatataaata aaataaaggg tgattaaaag atgatgaact 1381 tagttgaatt agagatctca aaaacacaag ctattaaaat atacttaccg tgccaaaaag 1441 aggatgtggg ttcatttgat attatatcag taaaatattt tagagaacaa atggagtatg 1501 acttatatgt taacgatttt gcggcagaag caataatatc gctaaaaaat atgctagaag 1561 aagcgttgaa ttttaagtta caaattcaaa acaaatattt agataaagga atagggtatt 1621 accataatat ttattataat aaattgtgga caactaacga ctcaagtctt actgatccag 1681 gagaagactt tattctttgg tcaactccaa cacatatagg tattgaaacc tttatttata 1741 atgttcaaaa taaaatatat attgaaatat gtcctattta caaatggaat ggtagttgcc 1801 cggaggatga agatgaaaat aaagacgaat atgtaccttt tgaggttttt ctcagtaact 1861 ataaaccgat tgatattatt gtcattgaca aaagtgttgc tgaaaagtgg tttaaacttt 1921 gctgtgagat gatagaggtg tttgaagcaa acgataagag atatttaaat gaaagaaaaa 1981 caaattaact cgtttgtggg gaggatatgt aaaattttgt gtaaataaga atgaaaagtc 2041 tccttcttgg caagaattac aaaataaaaa tcaaggagga gattttttta tgtcaacatt 2101 atcaaaagaa caagtaaaag aaataattaa gggtaataat ttccaaagtg ttgctgatgt 2161 aagcgcatat ctaaaagata tctttaagga tattatccaa gagcttctcg aagcagaact 2221 tgaggctaaa ttaggatatg caaaagatga tgtggaaaac aaaaatacag ataatagtcg 2281 aaatggctat tccacaaaga ctataaaaag tgaatttgga gaagttgaaa tccaggtacc 2341 aggagaccgc aaaggggaat ttaaacccca aattataccc aagtatcagc gtgatgtttc 2401 cggaattgaa gaaaaagtta ttgcattgta tgccagagga atctctaccc gggatataag 2461 tgagcaaatt gaagaacttt acggttttag tttgtcacct gaaatggtga gcaggattac 2521 ggatagaata gcaccttgag attagggaat ggcaacaaag accactggag cctgtataca 2581 cgtttgtatt catggatgca attcactata aagtgagaga tgaaggaaga atagtgaacc 2641 gagcggcata tgttgttatt ggtatcacag tagaaggaag taaagatatt ttaggaattt 2701 ggataggtga taatgaatca tcaaaatttt ggcttggagt attaaatgat cttaagaaca 2761 gaggagtgga agatgtactg atattctgcg ttgacgggct gacagggata aaggaagcaa 2821 taaatgcagc atatccaaag gcggaagtac agcggtgcat aatacatcaa ctcagaaatt 2881 cgtttaaata tgtgtcatat aaagatataa aagctttcag caaggacttt aaagaagtat 2941 accgtgcaat taacgaagaa gtagcaatgg agaagttttg tgaaatcaag gacaaatggg 3001 ggaaggaata cccatatgca atacgaagct gggaaaacaa ctgggatgtg ataagtccat 3061 tctataaatt tccagaagaa ataagaaaaa taatttacac caccaatatc attgaagggt 3121 tacatcgaca gtttcgaaaa gtaacgaaaa caaaaacaat gtttccaaat gacagttcac 3181 tggaaaagat gctttattta gggtcaatga atgtggtaaa gaaatggaca caaaggtata 3241 agaattggga taaggtacta agtcaactga tgattgagta tccaggcagg ttggagaagt 3301 atatttaaaa gcattcatcc ccaccgtcct caagggctcg ccctcattgc cggacgagct 3361 aaaccttaca aaataaaaaa attgggcgaa aaaaggtaaa gcaccccatt ataaacaatt 3421 ataaccaaat taaatcaaac ttatgtgtaa aacttaacaa aactggcaac aatatataat 3481 atcaaaagtg atttaggatt tacattctgt aaactttgaa aactaaaaat tgcttgccag 3541 gaaggattgc aaaatttctg atacacagaa ttatttatac tcccaatttt tgcacggcat 3601 ttaatattgc aatttataaa agaatgtaat tatggaaaac ttattgacat gcaattttgt 3661 taaatatata atttattata agattatcca tgtaatgttt tttacggttt tgaaatttct 3721 tctgcttttg cgcaaatatt tatcgacaca aattggatcc aacaaacaag ctgttgacct 3781 tgaagtccta agttccaggt tagtgatgag tttttattga agtgtttttt gttgttttgt 3841 ttgtgatttg tttgtgggag acaagttttt taagagaaaa atatatttaa acttattttt 3901 cttgaaatga ataagttttc aatttagggg agaatacata aaataaagat aatataaagt 3961 gaccggaaag ttcttttaaa tttaggggag aggatttcgt tttccaagag aacataaaga 4021 gaacttagga cctaaaggtt gacagctttt tataatttca tcggagttta tttttcaaat 4081 ataatttgca ggaaaaattt ctattgacac tcacaaaact catatataaa atataatcgt 4141 gtgttttata aacagaataa aaatttttca gcaataacca tatcagccgt gaagtgcttt 4201 cccggctgaa gttgttttta atggaggatt gaaatcatgt ctgtcaagta catttttgta 4261 accggcggtg ttgtttcggg gcttggaaag ggaataactg ccgcctcact gggaagactt 4321 ttgaaagcca gaggtcttcg agtcactatt caaaaatttg acccgtatat taatgtcgac 4381 ccgggtacta tgagcccgta tcagcacgga gaagtgtttg taacagatga cggggctgag 4441 acggatttgg accttgggca ttatgaaaga tttatagatg aaaacttgag caaaaacaat 4501 aatgtcacca ccggtaaaat atactggtcc attatcagca aggaaagaaa aggagatttt 4561 ctcggtggta cggtgcaagt tataccacat gtaaccaacg agattaaaaa tagaatttat 4621 ttagtgggca aagaaggaaa aaccgatgtg gtgattaccg aaatcggcgg tactgttggc 4681 gatattgaaa gcttgccttt tttagaggcc attcgtcagg tggcaagtga cgtaggcaga 4741 gagaatgtgg tttacataca tgttaccctt gtcccttacc tcagcaagtc gggagagctt 4801 aagacaaagc ccacccagca cagtgtcaaa gagttaagaa gcctcggtat acagccggat 4861 ataattgtat gccgtacgga aaagagactt tcaaaggaat tgaaggacaa gataggactg 4921 ttttgcaata tacccggaga atgggtaatt cagaatcttg acgccgagtc cctttatgag 4981 attcctttga tgctggagga agaaggactg gccaacattg tttgtgagcg tttgaatctt 5041 ggatgcgtta agccggatat gacagaatgg tgtgagttgg taaacaggca gaaaaactta 5101 agtaagtcct tgaccatagc tcttgtgggg aaatacgttg aacttcacga tgcgtatctt 5161 agtgttgtag agtctttaaa tcacggagga atatataatg acgccgaggt aaagataaag 5221 tgggtgaatt ccgaggaact tactgaagac aatcttgagg aaactctctg tgatgtggac 5281 ggaatactgg ttcccggcgg ttttggagac aggggtatag agggaaagat tcttgcagcc 5341 aaatatgccc gtgaaaacaa agttccgtac tttggtatct gccttggaat gcagatggcg 5401 gttgtggagt ttgcaaggaa tgtagccggg cttaagatgg ccaacagctc ggaatttgac 5461 gcaaacagtc cgcatcctgt aattgatctc atgccggaac agaaggatat agatgaaaag 5521 ggaggtacaa tgaggcttgg actgtacccc tgcaagatta taaaagattc ttctgcttat 5581 agaatatatg aaagcgagct tatatatgaa aggcacagac acagatatga gttcaacaat 5641 gaatacaggg agcttttgac ctcaaaggga ctgattctgg caggtttgtc tccaagtgag 5701 aagcttgtgg aaattattga actgaaagat catccatggt ttatcggagt tcagttccat 5761 ccggagttta aatcaaggcc gaacagaccc cacccgttgt ttaaggactt tataagggcc 5821 gcggtggaaa gacgttacaa acagcaggaa aataaaaacg attaatgata agaatgatat 5881 aaaaatcggc tgttccgtca atcggacagc cgattttaat gtatttgacg caatttaaag 5941 caatttctcc gttaaattat ttagctaaaa ttatctggct gaaattattt atctgtctta 6001 attgctttat agaatgcggc aaagcctccg aagagtattc cggttatcgg aaatacaatc 6061 cagcatatgt tccaaagctt gaaaacaaag ccaccgatta aaaagagggc ggttgcaatt 6121 ggccagatga ctccggcaac ggctccaatg actttattct gtcttaccct ttccggactg 6181 tactcgtcaa ttttcatcag ttttttataa gcttcagcgg tactggtgct tgttataaaa 6241 ataaatactg cggcggctat cattaaaagc atgatagata ctccgaaagc ggcgaaagac 6301 tcaaagattc caaaaacaag caaaacgacg acggagagta tgcaaaggct gacacccgtg 6361 atgatgggaa tattttgttt ttgagtaaca gatggcagtt ctgagtttaa aatatttttt 6421 aaacccggtg agatttcaaa ttttccctct tcaatgaact tgaacttttg aagtttgttt 6481 tccgtgtaca tgaagaggat taccgccgga attataaata aaaacagcac tataacagga 6541 agagcttctc ttgcattctg gggaagaaat ttaaaaatca gattgcgttc caccagcgtt 6601 gccaaaaata tcaatagtga aactcccaaa agaatcaaac cgacacctgt tgcaatcatg 6661 taagaagttt tttctttcag cgagataaat tccattgctt cttcattttc tattacggga 6721 tggttttcac tgtttgatga cggggaaatc cccatttcct ccagcagttc atcaatattg 6781 ccgaattcgg aaataacaat gccgattgcc tcattttcag gctttccgct ttttttaagt 6841 tcattgtact tttcttccat gttgtagtaa atctcgcttt ttagccgttc catttccttt 6901 gtttttggca aattggcaaa aatgttgtca agatagtttt taattgtctc cattgctttc 6961 caactccttt acgaatttat taattacctt tttggtcagt tcccattctt cgcacttttg 7021 tttgtagtat ttgattccct cgggagttat ccggtagtaa gttcttcttt tgccctgagt 7081 ttcttcccca tagtaggatt tgatatatcc gtttttttcg agacgtgcga aggcggaata 7141 aagcgttgtt tcttttataa cgtacagttc gtctgttttt atgcggatgt tctttgaaat 7201 ttcgtaccca taggaatcac cttctataag gaggcttagg ataatggtat cgacataacc 7261 ccgtattaca tcactgctaa tcaccgtaac acctccttta atattattac gtcagacgta 7321 ctttgtctgt cgtagtaata ttttatcaca attactacga caggtaaagt actttttgaa 7381 aaatgccaaa tttaaattct tttattacaa aattatgttc acccgtccta tatatagatt 7441 ttgatgaaaa taatttaatg tatagtataa ttcgttttga atttgtaaag aattatacta 7501 aacaataaaa gtttatattg taaaaaaaat tagggaatgg aaccattttg gaattgcaaa 7561 ttaatctcga aacggacaga ttatcgaata cggggggaat aatatcatgc tgaattcgtc 7621 aaataacacc ggcaagaaaa acatatttgt aaaagtactt acggcaatca ttctttttgt 7681 gtcagtcctg tcatgcgtcg ggttaatatc ctactcggag aatgtaagca gcgggcttgc 7741 agataatatt attcgtctgc atgttctggc aaacagtgat tctccggaag accagcagtt 7801 aaaaagggat gtcagggatg ttgtaataaa tttcatgaag gacaagctta aaaatgtaaa 7861 agatgtggaa accacaaaaa gaataataga agaaaactca gaagctataa aacaggtggc 7921 tctcagtgaa atcagaaggc agaataaaga ttatgacgta acggttatgt ttggaaaata 7981 tccttttcca accaaggttt atggggacat aagccttcct accggaaatt atcaggcttt 8041 gagagttgtt ataggtaacg gtgaaggtca aaattggtgg tgcgtgcttt ttccacccct 8101 ctgctttgtg gatgccactc acgggactgt gccggattcg gtaaaggatg atttgaaaga 8161 ggttttaagc gaagaggagt attatcttat tacagcgcag gatgaaggcg acgatattga 8221 aatcgagata aaatttaaaa ttgtggagat gtttcagaag tcaaaaataa aaattgcgag 8281 cgcaatgaat aaaatattcc aatgacgggt atgagtgtaa aattcatgca caggcgtgat 8341 tcgggctgac aaaatatatt cttgcaaaat attttgctgc ggactccggc atgtattgta 8401 acttccttca gcggatttta cggttggtgg caggttacga tactatgccg gggttttttg 8461 cttgtattta tctttataaa aaataaggat atatgtatat tgaagatata catgcatttg 8521 atatatactt ttaatatata ctttttattg taagacattt cttccttctg caaagcggaa 8581 cttttatatc cccaaaaacg aggtgagctg tatgataaat atgataagga ctttttttga 8641 aaacgggggc ttttatttaa ttgttattat gaggctcctt cttgcatgca tcctgggtgg 8701 gttgataggt tatgaaagag agagcacgaa caggcccgcc ggttttagaa ctcatatttt 8761 ggtatgtgtt ggctcagctc ttgtcatgat tacttcccag tatatttttg agagttataa 8821 aggaattact aaccttgatc ctgcccgtct cggagcccag gtaatcagcg gtatcggctt 8881 tttgggagcg ggaaccataa tccgtgaggg tgcaaatgtc aggggcctta ctaccgcggc 8941 aagcctgtgg gcagtttcct gtgtcgggat tgcagcaggt atcggttttt atgaaggagc 9001 ggttatatcc acaatagtta tttatattac acttattttg cttaaaagaa cggaaaaaca 9061 tattgccaga aaaaacaatc tgagcatatt ctatatacag actgaagatt tgcctggaca 9121 aattggtaca ataggctgta tatttggtaa gcataatata acaataagaa atatagagtt 9181 tatcaatgag aaaaaagatg aagaagtgtt gataaaattt gcagtaaaga tgccctctga 9241 tatgtctaaa gaaaaaataa tggatgagtt gcagagagta aacggagtga aaaaggttac 9301 aggccaataa aaccaatata caacaaactt ttttcaaaaa ctaaaaaata tttaacaaag 9361 tgtttgactt ttttcatgct ttcccctata attcatttgt ttacaatttt tttatgtttg 9421 cgaagctaga aagaggtgat taatggtgct aagaatgaac cagcttaaga caccggtttt 9481 tgatgctgtg aaaagatacg ttgaaagcaa tgtgatacag tttcatgtac cgggtcacaa 9541 gcagggagct ggtcttgagg agcttagaga gtatataggc gaaacagcgc ttaaaatgga 9601 tgcaaacggt atggaggatt tggattttgc caacaacccc acgggggtta tttatgagtc 9661 ggagatgctg gcagctcagg catttggggc tcagcatgct tattttttgg taaacgggac 9721 tacgtccggt gttcaggcaa tgattatgag tgcttgtgaa ccgggagaca aaataatact 9781 tccgagaaat gcgcacaagt ccacgatagg tggaattata ttaagcggtg ccgtgccggt 9841 gtatgtgcag cccgaaataa atgaaaaact gggcattgcc atgggaatta cggtggaaag 9901 cctgaaaaag gcaataaagg aaaaccccca tgcaaaagct gtgttcatta tcaatccgac 9961 ttattatgga atagcctcgg atttaaaatc cctcgtaaga attgcccaca ggcacgaaat 10021 ggctgttttg gtggatgagg ctcacggtgc gcatatgtct tttcatgatg attttcctct 10081 gacggcaatg gaagtcggtg ccgatatgag tgccgtaagt actcacaaaa cgggaggttc 10141 actgacacaa agttctttgc tgcttttaag gggaaacatg atcagccctg aaagagtaaa 10201 acaggtttta aaccttacat atacttccag cgcatcatat cttttgatgt gttcccttga 10261 cattgcaaga aaacagcttg ccacaaaagg aagcgacatg ttggaggaaa ctttgaggct 10321 tgccagaatg gcaagagagg agataaacaa aattgaaggt ttgtatgcct tcgggaaaga 10381 acttatagga acaccgggat gtcatgattt tgatgaaaca aaactgggta tatgtgtatc 10441 aggcttggga tataccggat atgagatgga agcgaaactc agaaaagaat acaatataca 10501 gattgaaatg tcggatcttt caaatatact ggcaatagta agcataggtg acagggaaga 10561 aaacttggtg gcccttataa acgcactgaa agatattgcg gccaaaacag agaaaaagga 10621 atatcccaaa ccgccgatta ttcctccgac tcccaaaatg atagtttcac ccagggacgc 10681 tttttacagc cccaagaaaa tagtcccgtt ggacaaatct gtgggagaga tatcgggaga 10741 aatggttatg gcttaccctc cgggcatacc ggttgtatgc atgggtgaga gaattactca 10801 ggacatagtg gattacataa agatactgaa ggagcagaaa acccagctgc aaggcacggc 10861 ggatccatac attgatcata taatggttct tggtgttgat tgacggatta caatcattgt 10921 ttactccgat tttaattgca aaaccaatca cttttttggt ctaattgtcc ttattcctga 10981 atagaaatag gattatgaca ggaaaatgta catggcataa gcacatgggg acaaggcaaa 11041 aaggagtgat tggttttgaa tattttggag tcggctctgc aaaacagcaa taaggataac 11101 aaggtaaaat acgggttaag tgagaaagag gcggagaaaa ggtataaaaa gtatgggcca 11161 aataccttgg ctgaaaagaa aaagatatct ccttttaaga tccttattgc gcaattcagt 11221 gactttatgg tgttgattct tctggcatcc accttgattt cagccttcat gggagaattg 11281 acagaagcaa ttactattgt gtccattgtt attcttaacg cacttatggg atttgttcag 11341 gagtatcgga ccgaaaaaac cctggaggcg ttaaaaagcc tggcggcgcc ggtggcaaag 11401 gtggtaaggg acggaaaaac cgtggaaata cctgcggaaa aagttgtgcc gggagatctt 11461 gtggtgcttg agaccggcga caggattccc gcggatgccg ttctgataga atcccatggg 11521 ctgtgtgtgg atgagtcttt gctgaccggt gaatccgtgc ctgtggaaaa acaagttgaa 11581 gccggcggca gaaaaaatgt gaacgccggt gaaagaaacg gcacggtata tatgggaacg 11641 gttgtaacca gcggaagagg gaaagctgtt gtctgttcca ccggaatgat gaccgagatg 11701 ggaaaaattg ctgacatgat acaaaatata gagacggaac agacacctct tcagaagaga 11761 ctggatactt tgggtaaata catagtttac ggatgcctta ccatatgtgc aattgttact 11821 gtaaccggca tattaagggg agaaaaactc tttacaatgt ttctttcagg cataagcctt 11881 gcggttgccg cggtgccgga agggcttccg gccatagtaa caattgccct ggctttgggt 11941 gtccagagaa tgtataaaag aaacgctttt ataagaaagc ttccggccgt ggaaactttg 12001 gggtgtgcca gcgttatatg ctcagacaag acaggaactt taacggagaa caagatgacg 12061 gtaaggaaaa tttataccaa cggaggcttt gtggaggtta agggaagccc attaagcagc 12121 gagggcgaat ttatctcgct gaaaagaaaa atagatccca aaaacaacaa ctctctgaga 12181 cttactcttg aaatcggggt tctgtgcaac aatgcatcaa tggtaagaat aaaagaaaac 12241 aggtctttcg gcaaaatagc atctctggtt ccaaggacgg aaaaatggga acttagcgga 12301 gatccgaccg aagccgctct tcttgtggcc ggagcaaaag ggggatgtac ccaggagctg 12361 ctcaatgaaa gttatacgag aattgatgaa ataccttttg attcagacag aaagtgtatg 12421 tcggtaatat gcgaaaatga aagaggagag gtttttgttt tcaccaaggg tgctgcggat 12481 gtaataattg aaaaatgcag caagatatac acctcaagag gaattgtgga tttggatgag 12541 gccggagtca aaagggtgct caaagcaaac gatgatatgg caaaggaagc tctgagagta 12601 ttgggagtgg cgtttagaag gcttgactcc aaaaactaca ggctcgatga tgtggaaaag 12661 gatcttgtgt ttgcaggatt gataggaatg atagacccgc cgagaaaaga ggcgctggat 12721 gcggtacgga aatgcaagct tgcgggaatc aagcctgtga tgattaccgg agaccataaa 12781 ataactgcgg cggctattgc aagggagctc aatatagctt ctgaaggtga cagggtactg 12841 acgggagccc aattggagca gatggatgac aagaggcttg aagaactggt ggatgaagtg 12901 tcggtgtatg ccagggtttc acccaggcat aagctgagga tagttcgggc gctcaaaaaa 12961 cgggggcacg ttgttgccat gaccggagac ggtgtaaatg atgctcctgc aatcaaggaa 13021 gcggatattg gagtggccat gggaataaca ggtacggatg ttacaaagga agcatcttca 13081 atgattctcc ttgatgataa ttttgcaaca attgttgcgg ctgtggagga aggaagggtt 13141 atatacaaca acattcgcaa atttatccgg tatatgcttt cctgcaacat cggagaagtg 13201 ctcactatgt ttgtgggaac cctcataggc cttccgctgc ctcttcttcc gattcagata 13261 ttgtgggtta atctggttac ggacggactt ccggcaatag cactgggact tgaacctggt 13321 gacaaagata ttatgatgag gcctccgaga ggagcaaagg aaaacatatt ctccaatgga 13381 ctgtggcagc ttataatatt ccggggagct cttatagggc ttagcaccct ggcggtattt 13441 gcaagcatac tgaactttac gtccaatgtt gatgttgcga gaaccgcagc ctttgtaact 13501 ctggttataa cccagcttat acatgtgttt gagtgtaaat cggaaagaaa aaatatattt 13561 gaaataccgc ttttcaacaa tatacctctt gtgctcgcag ttttgtgttc gcttgtaatg 13621 atactggggg ttgtgtatat accgttattc cagggaatat tcaagaccgt acctttgtct 13681 ttaaatgact ggattctgat aggaggattt tcggcattgg gtcctgtaat ttcaagtttt 13741 ttcaaagtta actacaaata cattagaaga tagaaaaaag caaaaaaaaa tttaaaattt 13801 taaccttttt gtaaccattt tatattagct ttaatatata ataaactcga agtacttcaa 13861 tagaaattaa ataaagcaat gctgttcttg tatacaggaa acggcggaaa tagtatttta 13921 agaaagttga ccgaatgaag gcgatgtagt ttcggaaacg gagtttgtac tgccgttgaa 13981 atattgacat tgaaagctac ataacagtat tggtatcatc ataattaata atcacgtctt 14041 gctgtggatg gggcattgtt gtaagcagat tttgtactta aggggtacta aaaaatacag 14101 tattgaatac ttcattactt ttacacttat tctcatatat tcgaaggcaa ttttttataa 14161 agtttatcgc gcgcgcattt aatatataat gtatgaacat ctgtatatgt gttataaaaa 14221 gcaccagggg gaaaagtttt gagaatcatt ggttgtgtaa attttcagat gtaaagtccg 14281 cacataacat gaaatataat caatcagctg cttgcagact gatatgagag agaaataggt 14341 tctgcttgtg gtgcgaggca tgcgtgggtc ggaaaagcag ctttgggtaa gcacgatgag 14401 cagtatctgc caaaaagtat gatatgattt atgtttaagt gggagtttaa tgttgtacaa 14461 tattaaaaac gaagcaaaaa gtaaagaata aagaaaagca aaagaaaaat aaagaaaaaa 14521 gaacggcgtt aagagaatgg gaaaacggtg acatgagttt taagcaaatt gggtgatcac 14581 ccccggcaat atatactgaa tgtgttaatg atgttaaaga agttgtttta cagcagcaat 14641 cctcctataa aacttacatt tgcccctatt attgggactc acattttttc atataatttc 14701 ctccgataaa tttaattgga caacgttaaa cgttgcactt ttaaagcacg aattgcgttg 14761 tctttatttt attgtcttta tttttttata ttccacactt ttctttgttt tttattattt 14821 ttgagactgc aatattatgg taaaaaatgt atttaaaaaa tttgaaatta tatttgtagc 14881 aattggatta attttttatg taaaatctgg attatacata ccttttctgg tattataatg 14941 atagtattaa atttttcaat aaatgacttt ttaaattgtc cggtattgct gaattcgcct 15001 tggcatcact aactttggaa tgtgtttact gtttgaaagt aaaataaaac agttgggaaa 15061 aatgaaaata taggactctg cttttttatt atgttaaggt ataataattt aataaaagat 15121 tcaatgtatt gtaataaatg tatttggatt atttgggacg tataataact gttaaaaata 15181 aaaaacttga ttgtattatt ttctctgaaa aggaatgatt tctttatggg ggctaaaata 15241 ttaattgtag aagacgagaa cgatattgga agctttatgg taagaaccct gaacagaaag 15301 ggctttaacg caatccgggc agaaacggga aaagaagcgt taagacttgt ggagttggag 15361 aagcctgaag ttatattgct ggatataatg cttcctgata ttgacggatt tgagatatgc 15421 aaaagagttt ccagacaaca tcctgaaata tcaataataa tggttacggc aaggggagaa 15481 gatgtagaca agataagagg cctggagctt ggagctgatg attatataat caagcctttc 15541 aatcccatgg agctgatagc gcgaataaaa gcggtaatca ggaggacaaa caaaccggat 15601 aattcgaatg aagttgtaat aggtccttta agaattgact acaactgccg gcaagtgtac 15661 aagaatggag tggtcctgga cctgacaccg agagaatttt gtttgctgaa agtctttgta 15721 aataacttga acaaggcttt gaccagagat gaaattttaa actgggtgtg gggcgaagac 15781 tatattggag ataccaagac cgtcgatgtt cacgttcggc gacttaggga gaaaatcgag 15841 gatgacccgt cacatcccaa atatatagag acagtttggg ggaaagggta tttatttaga 15901 aagggagatt gatttgcggg gaatcagggg cagagttgtc ggaacttatc tgattattac 15961 ttttctgtcg gtggtgatat ttgaaattat tctcatttac gggcttgtag agttttatta 16021 ttcaagtata gaggctgaac tgagtcgttc cgcacagtca ataatccata attacagcca 16081 atatttcaat aattatgatt tacacaggga tgcaaaagta ttgcttgaat ccatgcccgg 16141 caataccgtg gcccaagtcc agataattga tgacaaaggg gttttgattg ctgattcgat 16201 agagccttcc atggaaggaa aaaagcttga taattacgat gtcaatatgg ccttgaatgg 16261 gaaagaggct gcgtggaaag gaaaaatacc catgacgggt gaaccggttt tttcggtttc 16321 ttttcccata atccgtcctg acagcgaaga agtgattgga atagtaagag taattacgac 16381 tctttccgat gtaaatgaaa tattgaaaaa tcacataatc attctcattt ctttgggact 16441 ttgcatagtt tttctgattt ttcttaccgg actggctttg accaatacca ttataaaacc 16501 ggtcaaggaa ataacatctg ctgcgaaagc catggcccag ggcagattcg acgtacgggt 16561 atccaaaagg tatgatgacg aaattggaga acttggagat accttgaatt acatggcaca 16621 ggaagtggcc aaccaccaaa aaatgaaaaa tgactttatt gcgtcaattt cccatgaact 16681 tcgcactcct ttgacatcaa taatggggtg gataattaca atcaattccg gagacattga 16741 cagcaaagaa gaactgaaag aaggattgga tataattgaa agagaaagca aaagactggc 16801 ggagcttgtg gatgaacttt tggatttttc aaaatttgat gccggaatca ttacgttaag 16861 gaagagtgtt gtaaatttgg gtgagctttt gaaatacata aaaaggcaaa tggagcctcg 16921 ggctgagcga aaaggcataa ccatgacgat agacgtggat gagcatctgc cgttgattga 16981 agctgacgaa aacaggctta aacaagtgtt tataaatatt attgataatt catttaaatt 17041 tacgcaaaaa ggcgggtata ttgatattat aggcagaaaa aatgaaaacg gtgttttgat 17101 aagaatagag gacagcggat gtggtatacc ggaggaagat ttgccgagag ttaagcaaag 17161 gtttttcaag ggaagcaatg ttgtttcggg aagtggtttg ggacttgcca tctgtgatga 17221 gattgtaaga cttcacaatg ggaaaataga catagaaagc actgtcggaa aaggcaccag 17281 agtagatgtg atacttccgg tttgagttta aatttaacca ttttgtaatc tttttttttg 17341 cgttataatg taagattata atatgcacaa tcgaaatttt acacgttgtt atagagaggt 17401 taaagatggg cgaaatacgt aattacataa aattaatatt aataggcgtt gcaattttag 17461 ttacggttac aatgataatt tcggcgacag gttgtgacat tctgccgcct cctgccagtg 17521 ccataaaacc tccgcagtat ataaatgctt caagcggaga agttgaagat ccaaaggcaa 17581 tagcacggcg ttttttgact ccggggacgt atttttcata tcctacccat cccaggaatg 17641 ctgatgccgt ttgggaggtt gacattgacg gagacggtcg ggcggagttg ttgacgctgt 17701 acaggggaag ggatcttaaa aacaacggag ttgaagtatt tggcgtattt atattaaagc 17761 aggatgaaaa tcatgaatgg caaatttttt gggagcaaca atataccgca aatattgtgg 17821 ctttggattg ggcggatgtc agagatatta ccggagacaa ctatcctgaa ttgcttattg 17881 gctggaatat tggatatagg ataggcagta atcttgatat atattctctg aaggaggagg 17941 acaagcccag gctgatttcc agcataaagt attctgaaat tgtaggcatt gaagacagaa 18001 gaggtattaa caacgaaatc aagcctgttc tgatgatgta tctgtttgat gaggaaatgt 18061 acgagtcgga gaatgagggc agaaaagatg aatatttcca catattgcaa tgggattaca 18121 aggagtggat gggaggcgga ctggtagaag ctgatgattt ctacgcatcc tattatgtga 18181 atgttattga ggatttaaaa gagagcctgg agagatatag caacaattac tatcaatggt 18241 atatccttgc gttggctcag ctgagatcag ggaagcccat ggatgcactc gaatctgtcg 18301 gcaaagtttt ggaaattacc caaagatata ttaacaggca cggagaaagg accgtcaaag 18361 aggtttttaa atatcagaaa aaagcaagaa tactcaaagc ggcagcatat atcaaaatgg 18421 gaaaatatca tgagagcata attgaatcca actatgtgct gggcaattat gatattgatg 18481 acaaggaact tgcccagatc tatctgaatt tgggatatgc atatgcaggc cttaagcagt 18541 atgaaatggc cggcacgtat tttaacaagt catgtgaaac ggctgaaact gtttataaag 18601 agggtacggc attgtatttg ttgaattcgt acaaggcaaa aaaacagttg gaatacataa 18661 agccctttct gacatcctcc cactaaaaac agccttggat ttattcgctt ttcctgataa 18721 aataattaag atggtcaatt acatcaggat ggattatctt ctttcaagat ttaataaaag 18781 gagtgaattt atgaatcttc ttgacaggct ggaaagaaaa tttgggaagt ttgccataaa 18841 gaggcttccg atgtacattg tcatactgaa tttgttcgta tatatgttga acttacttga 18901 cagcagatac ataaattatt tgtatcttat tccgtcccgc gttcttaaag gcgagatttg 18961 gaggttggta acatatattt tcattccgcc tatgacttca atggaagatg ttttgtggct 19021 gttttttgct atatattttt actatatcat ggcaaccgga ctggagaatg aatggggcag 19081 ctttaaattc aacctgtatt atttgcttgg catgattggc acaaccattg cggcgttttt 19141 tattccgctg cctgctacgg cgaactttat aaatttatct atttttcttg catttgcata 19201 cttgtatccc gattatcaga tctatttgtt tttcattatt ccgatcaaaa tcaagtattt 19261 gggttatatt tcgtggattt tgattatcta tgagctttta acagtgccgt tgattatgaa 19321 attttatata atcataccaa tactgaattt cttcatattc tttggaaagg atatttttgt 19381 aagatataaa accaggggaa aatcggtata cagaagaagc aaatatatgg gtaccttcag 19441 ggaaaagtcg tattttcaca agtgtacggt gtgcggtgtc acggaaaagg ataatccgga 19501 catggagttc agatactgca tgacttgtga ggggcattat gagtattgca tggagcatct 19561 taaaaaccat gaacatataa aaaagtagaa aagctttcaa caactttaaa attgggaatg 19621 caatcaaagg tatttgttga tgaaaaaaga agtaacggat atatatagaa tatttttggg 19681 atgaaaataa taaattttat catgacggat ttttggtata tatgctataa tattttatat 19741 cgtgatattt ttgtctagtg gacaaatgtt aaaaaattaa caaaacaaac aggaggtaag 19801 agtgaatgaa aaagattgca agaaaaatat caatgttact ggtcgttgca cttttggcgg 19861 tatcgatggt ggcctgtacc agtgatgaaa ttgcattaat tgaagccatg tccaagacat 19921 cagaaatttc atcctatgaa ggaaattcaa aaattcagtt aagttttaaa ggccagggat 19981 tttcggaaaa aacgcagaaa gttttcgatt ttctggcttc atatgttgac ggattcactt 20041 ttgaggcaaa tcagaagtat tcgtccaacg atgaaaagac aaaagcaacg cttgccatgg 20101 acggaaatgt ggatatgcaa ggtttgagcg ttaaatataa atattggacc gatatggact 20161 ttacaaccga gaatcctagc ttaatacaga ttgttgaact tccgccggca attacccagc 20221 cgatgtttac ctttgccaac acaggaacaa aaaaatatat tactattgat tacggtagtg 20281 tattgtctgc ggaaaataac gggggcatat ctcttaaccc ggagaatttg gcaaaaaaca 20341 gtgttgagct gcaggaaatg ttgctggact ttgtaaaaac aacagccaag gactttgacc 20401 ccggtatggt ggcagtaacc aaaaaaggtt ccgctgttac tgacaaggga gaaaaagtta 20461 cggaatacga attgaaattg gatgatgctg cagctaaaaa attattgcat gcttttataa 20521 atgatgtcat attgcaggaa gatactatag agtttggcaa aaagtatatg gaagctgcca 20581 taaatatgta tgatttcccg gaagaggaaa aacaagaggc attggacgaa atcaacaaag 20641 gtctggatga atttgcatcc cagcttcctg catacaggga cagtgttaca caagtttttg 20701 agtcgataaa agacgtcaaa ttctttggtg acaaaggttt ggtagcaaaa tactacataa 20761 acaatgacgg cttccttgta ggaggaaaat catccattga cattaagata aaaatggcag 20821 attttgcggc attattgggt gacaattttg acgaaaaaga taaaaatgga gtgctttatt 20881 taacaataga cgctgaaagt tctgtttaca acataaataa agaagtatcc atagaacttc 20941 cggaaataac tgaagaaaat tcctttgatg ttttgaaagg atttatgccg atactgtctg 21001 gtattggttc tgctccgggt atcggtgaag aggattatac atatgatatt cctgctttgt 21061 cagacggaat taatgttgtt atgaatggaa aagtagtata tttccctgat gtaaagcctg 21121 aaaacgtcaa tggaagagtg ttggttccaa taagaaccat atccgaggaa atgggagcgg 21181 aagtaaccta taatgatgca acaaagcagg ttcttattgc caaggatgat acggaaattc 21241 ttcttacaat tggttcccag gaagcttacg ttaacggcga gaagataatg cttgatgtac 21301 cggcaatgat tattgaagga cgtacaatgg ttccgttaag attcatatct gagagtatga 21361 atgcaacggt tgaatgggac ggagaagctc agatagtata catattttat taatacttaa 21421 ttgatgcgta aaataaagtt ttaagaagct tttttatccg aagagaaagg ataaccgttt 21481 atagggcgga tatcctttc //