LOCUS NZ_ACVX01000049 15065 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00052, whole genome shotgun sequence. ACCESSION NZ_ACVX01000049 NZ_ACVX00000000 VERSION NZ_ACVX01000049.1 GI:256005924 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000049. FEATURES Location/Qualifiers source 1..15065 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene complement(115..321) /locus_tag="ClothDRAFT_2730" CDS complement(115..321) /locus_tag="ClothDRAFT_2730" /inference="protein motif:PFAM:PF08984" /note="PFAM: Domain of unknown function DUF1858; KEGG: gbm:Gbem_1902 hypothetical protein" /codon_start=1 /transl_table=11 /product="protein of unknown function DUF1858" /protein_id="ZP_05430869.1" /db_xref="GI:256005925" /db_xref="InterPro:IPR015077" /translation="MKITKDMIIADVLQMDRGTAPIFINNGMHCLGCPSSMGESIEDA CAVHGIDADKLVKELNEYFEKKEV" misc_feature complement(154..315) /locus_tag="ClothDRAFT_2730" /note="Domain of unknown function (DUF1858); Region: DUF1858; pfam08984" /db_xref="CDD:149904" gene complement(456..1013) /locus_tag="ClothDRAFT_2731" CDS complement(456..1013) /locus_tag="ClothDRAFT_2731" /inference="protein motif:TFAM:TIGR00723" /note="TIGRFAM: hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit; PFAM: Fe-S type hydro-lyase tartrate/fumarate beta region; KEGG: sun:SUN_0574 fumarate/tartrate hydratase, beta subunit" /codon_start=1 /transl_table=11 /product="hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit" /protein_id="ZP_05430870.1" /db_xref="GI:256005926" /db_xref="InterPro:IPR004647" /translation="MKYIIEAPLTGEKARELRAGDVVSINGTIYTARDAAHKKMVNLI QEGRPLPFDIRDQIIYYVGPCPAKPGEVIGSAGPTTSSRMDAYAPLLIKLGLKGMIGK GLRSEAVVDAMKSYGAVYFGAIGGAGALIAKSIVAEELIAFPELGTEAIRKLTVKDFP AVVIIDSYGNDLYKIGREKYRIVSP" misc_feature complement(474..1013) /locus_tag="ClothDRAFT_2731" /note="Fumarase C-terminus; Region: Fumerase_C; cl00795" /db_xref="CDD:186192" gene complement(1044..1886) /locus_tag="ClothDRAFT_2732" CDS complement(1044..1886) /locus_tag="ClothDRAFT_2732" /EC_number="4.2.1.2" /inference="protein motif:TFAM:TIGR00722" /note="KEGG: cha:CHAB381_0023 fumarate hydratase; TIGRFAM: hydro-lyase, Fe-S type, tartrate/fumarate subfamily, alpha subunit; PFAM: Fe-S type hydro-lyase tartrate/fumarate alpha region" /codon_start=1 /transl_table=11 /product="hydro-lyase, Fe-S type, tartrate/fumarate subfamily, alpha subunit" /protein_id="ZP_05430871.1" /db_xref="GI:256005927" /db_xref="InterPro:IPR004646" /translation="MRTIHVDSITEAVEKLCMDSNYYLNDDIINGLEKGLEKEESDNG KEILSKIIENAQIAREKAVAICQDTGMAVVFMDIGQDVHITGGNLTDAINEGVRRGYE KGYLRKSVVNDPIERINTKDNTPAVIHYNIVDGDKIKITVAPKGFGSENMSALKMLTP SQGIEGVKNFIIETVEKAGPNPCPPIVVGVGIGGTMEKAAFLAKKALLRPIDKRNDIP YLKELEEEMLERINRLGIGPSGLGGRITALGVNIEVFPTHIAGLPVAVNINCHATRHA EIII" misc_feature complement(1047..1886) /locus_tag="ClothDRAFT_2732" /note="Fumarate hydratase (Fumerase); Region: Fumerase; cl00851" /db_xref="CDD:186222" gene 2198..2608 /locus_tag="ClothDRAFT_2733" CDS 2198..2608 /locus_tag="ClothDRAFT_2733" /inference="protein motif:PFAM:PF00582" /note="PFAM: UspA domain protein; KEGG: har:HEAR1623 sensory histidine kinase in two-component regulatory system wtih KdpE, regulation of potassium translocation" /codon_start=1 /transl_table=11 /product="UspA domain protein" /protein_id="ZP_05430872.1" /db_xref="GI:256005928" /db_xref="InterPro:IPR006016" /translation="MKGVFILSSPQNILVCVTQQITCERLILKAAELRNELKGELFVI HVAKNEWNFLDNIKEGEALEYLFKISKSVGANLSVLKSDDIVQTIVNFAKENKITHIV MGESPNDHKENNFYNELKRLLSNVEILVIPQAGI" misc_feature 2234..2593 /locus_tag="ClothDRAFT_2733" /note="USP domain is located between the N-terminal sensor domain and C-terminal catalytic domain of this Osmosensitive K+ channel histidine kinase family. The family of KdpD sensor kinase proteins regulates the kdpFABC operon responsible for potassium...; Region: USP_OKCHK; cd01987" /db_xref="CDD:30174" misc_feature order(2243..2251,2333..2335,2504..2509,2513..2518) /locus_tag="ClothDRAFT_2733" /note="Ligand Binding Site; other site" /db_xref="CDD:30174" gene 2800..3075 /locus_tag="ClothDRAFT_2734" CDS 2800..3075 /locus_tag="ClothDRAFT_2734" /inference="protein motif:PFAM:PF00216" /note="PFAM: histone family protein DNA-binding protein; SMART: histone family protein DNA-binding protein; KEGG: DNA-binding protein HU" /codon_start=1 /transl_table=11 /product="histone family protein DNA-binding protein" /protein_id="ZP_05430873.1" /db_xref="GI:256005929" /db_xref="InterPro:IPR000119" /translation="MNKTELINSIASKSGLSKKNSEAALNAFIASVEEALKKGDKVVL VGFGTFEVKNRAARKGRNPQTKEEITIPASKAPVFKAGKGLKDIVNK" misc_feature 2803..3060 /locus_tag="ClothDRAFT_2734" /note="Integration host factor (IHF) and HU are small heterodimeric members of the DNABII protein family that bind and bend DNA, functioning as architectural factors in many cellular processes including transcription, site-specific recombination, and higher-...; Region: HU_IHF; cd00591" /db_xref="CDD:29683" misc_feature order(2803..2811,2875..2877,2920..2922,2926..2928, 2932..2937,2944..2946,2956..2958,2962..2967,2971..2973, 2980..2991,3019..3021,3031..3033,3037..3039,3046..3048) /locus_tag="ClothDRAFT_2734" /note="IHF - DNA interface; other site" /db_xref="CDD:29683" misc_feature order(2803..2808,2815..2817,2824..2826,2836..2838, 2878..2880,2887..2892,2899..2904,2914..2928,2935..2940, 2953..2955,3019..3024,3034..3036,3040..3042) /locus_tag="ClothDRAFT_2734" /note="IHF dimer interface; other site" /db_xref="CDD:29683" gene complement(3135..3944) /locus_tag="ClothDRAFT_2735" CDS complement(3135..3944) /locus_tag="ClothDRAFT_2735" /inference="protein motif:TFAM:TIGR02875" /note="KEGG: sse:Ssed_1032 response regulator; TIGRFAM: sporulation transcription factor Spo0A; PFAM: Sporulation initiation factor Spo0A ; response regulator receiver; SMART: response regulator receiver" /codon_start=1 /transl_table=11 /product="sporulation transcriptional activator Spo0A" /protein_id="ZP_05430874.1" /db_xref="GI:256005930" /db_xref="InterPro:IPR001789" /db_xref="InterPro:IPR012052" /db_xref="InterPro:IPR014879" /translation="MKGEISVLIADDNVEFAKLLKEYMNQYEDIEVLELAKDGLQAIE MIISKKPDVVVLDIIMPNLDGLGVLERLSTMQLEYRPIFIMLSAIGQDVFVQRAVNLG AEYYIIKPFDVEVLVTRIRQLYREKTTSVFSNKPIIKNSELLSSNVDKNYDLEVEVTN LMHEVGIPPHMAGYQYLREAVIQTVKNSKVFTSVTKNLYPAVAEKYNTTPQKVERAIR NAIESAWARGNPDTVDALFGYTINYSKGKPTNSECIAMMADKIRITMGLRP" misc_feature complement(3150..3932) /locus_tag="ClothDRAFT_2735" /note="sporulation transcription factor Spo0A; Region: spore_0_A; TIGR02875" /db_xref="CDD:131922" misc_feature complement(3576..3923) /locus_tag="ClothDRAFT_2735" /note="Signal receiver domain; originally thought to be unique to bacteria (CheY, OmpR, NtrC, and PhoB), now recently identified in eukaroytes ETR1 Arabidopsis thaliana; this domain receives the signal from the sensor partner in a two-component systems...; Region: REC; cd00156" /db_xref="CDD:29071" misc_feature complement(order(3615..3620,3627..3629,3684..3686, 3750..3752,3774..3776,3909..3914)) /locus_tag="ClothDRAFT_2735" /note="active site" /db_xref="CDD:29071" misc_feature complement(3774..3776) /locus_tag="ClothDRAFT_2735" /note="phosphorylation site" /db_xref="CDD:29071" misc_feature complement(order(3750..3758,3762..3767)) /locus_tag="ClothDRAFT_2735" /note="intermolecular recognition site; other site" /db_xref="CDD:29071" misc_feature complement(3612..3620) /locus_tag="ClothDRAFT_2735" /note="dimerization interface; other site" /db_xref="CDD:29071" misc_feature complement(3159..3476) /locus_tag="ClothDRAFT_2735" /note="Sporulation initiation factor Spo0A C terminal; Region: Spo0A_C; pfam08769" /db_xref="CDD:149736" gene complement(4092..5363) /locus_tag="ClothDRAFT_2736" CDS complement(4092..5363) /locus_tag="ClothDRAFT_2736" /inference="protein motif:TFAM:TIGR01356" /note="TIGRFAM: 3-phosphoshikimate 1-carboxyvinyltransferase; PFAM: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase); KEGG: hiq:CGSHiGG_10170 3-phosphoshikimate 1-carboxyvinyltransferase" /codon_start=1 /transl_table=11 /product="3-phosphoshikimate 1-carboxyvinyltransferase" /protein_id="ZP_05430875.1" /db_xref="GI:256005931" /db_xref="InterPro:IPR001986" /db_xref="InterPro:IPR006264" /translation="MLLKVRKSKASGNVRIPGSKSHTIRALFFASLAEGKSEIQSPLI SDDALSAVEVCRALGAKIEKEDDKYVVEGFGGNPEVPEDVINVGNSGTTLRFGIMTAA LGDGCSVFTGDRQIRQRPLGPLLCAINNLGAEAFSTRNNGRAPVVVKGKLKGGRTEID SVTSQYLSSILINSPLIPLDTEVIVTRLNEVPYVDMTLWWLDKLGIKYENHDYKTFYI KGGQRYRPLNVTIPGDFSSATFFAVQAAISGEEFVLDNLDMTDPQGDKMVFSILEDMG AKVKVEGKSVRIKGCELVGREIDMNAIPDALPAMAVAGCFAKGETKLLNVPQARIKET DRIHVMCEQLKKMGADITELEDGLVIRESRLKGCKLEGYGDHRVVMSLAIAGLNAEGE TVIDTAEAVNVTFPDFVNFLSRCGADISTCE" misc_feature complement(4104..5363) /locus_tag="ClothDRAFT_2736" /note="3-phosphoshikimate 1-carboxyvinyltransferase; Provisional; Region: PRK02427" /db_xref="CDD:179423" misc_feature complement(4122..5333) /locus_tag="ClothDRAFT_2736" /note="EPSP synthase domain. 3-phosphoshikimate 1-carboxyvinyltransferase (5-enolpyruvylshikimate-3-phosphate synthase) (EC 2.5.1.19) catalyses the reaction between shikimate-3-phosphate (S3P) and phosphoenolpyruvate (PEP) to form 5-enolpyruvylshkimate-3-...; Region: EPSP_synthase; cd01556" /db_xref="CDD:30129" misc_feature complement(order(4662..4673,5307..5318)) /locus_tag="ClothDRAFT_2736" /note="hinge; other site" /db_xref="CDD:30129" misc_feature complement(order(4155..4157,4233..4238,4356..4358, 4365..4370,4380..4382,4449..4451,4785..4787,4794..4796, 4869..4877,5007..5009,5088..5093,5097..5099,5289..5291, 5301..5306)) /locus_tag="ClothDRAFT_2736" /note="active site" /db_xref="CDD:30129" gene 5577..6503 /locus_tag="ClothDRAFT_2737" CDS 5577..6503 /locus_tag="ClothDRAFT_2737" /inference="protein motif:PFAM:PF00072" /note="PFAM: response regulator receiver; SMART: response regulator receiver; KEGG: pat:Patl_2089 diguanylate cyclase" /codon_start=1 /transl_table=11 /product="response regulator receiver protein" /protein_id="ZP_05430876.1" /db_xref="GI:256005932" /db_xref="InterPro:IPR001789" /translation="MDGTVLLIDYFEYEREKTKIIFDNIGEYDFIEVENLKKFYSIFK DLDSITLIIMDIAFPVEKEGLEVLSAIRNNSRTANTPVIIATKSDNPGYRHAALKFKV SDYILKPYPTKRLENSVRSVLKICQRFRYEFDSAHVISMSIEDYISKEFKVASRAGQS LSVILMAPVGLEKEPSEQSTQISSELQDQIQNLAIEKVKLSLRSTDTAILNANRDILV ILPFTNAAGAQKVLQKIQDNVREGLKSLNINYDDYYYAVSVTFPNDGKTFQSLMEKAV KKVEDKIMLEKITSIGVNILDNARNSYKKFNK" misc_feature <5580..6413 /locus_tag="ClothDRAFT_2737" /note="Response regulator containing a CheY-like receiver domain and a GGDEF domain [Signal transduction mechanisms]; Region: PleD; COG3706" /db_xref="CDD:33501" misc_feature 5592..5945 /locus_tag="ClothDRAFT_2737" /note="Signal receiver domain; originally thought to be unique to bacteria (CheY, OmpR, NtrC, and PhoB), now recently identified in eukaroytes ETR1 Arabidopsis thaliana; this domain receives the signal from the sensor partner in a two-component systems...; Region: REC; cd00156" /db_xref="CDD:29071" misc_feature order(5601..5606,5739..5741,5766..5768,5832..5834, 5889..5891,5898..5903) /locus_tag="ClothDRAFT_2737" /note="active site" /db_xref="CDD:29071" misc_feature 5739..5741 /locus_tag="ClothDRAFT_2737" /note="phosphorylation site" /db_xref="CDD:29071" misc_feature order(5748..5753,5760..5768) /locus_tag="ClothDRAFT_2737" /note="intermolecular recognition site; other site" /db_xref="CDD:29071" misc_feature 5898..5906 /locus_tag="ClothDRAFT_2737" /note="dimerization interface; other site" /db_xref="CDD:29071" gene 6622..7758 /locus_tag="ClothDRAFT_2738" CDS 6622..7758 /locus_tag="ClothDRAFT_2738" /inference="protein motif:PFAM:PF01170" /note="PFAM: putative RNA methylase; THUMP domain protein; KEGG: gur:Gura_3994 putative RNA methylase" /codon_start=1 /transl_table=11 /product="putative RNA methylase" /protein_id="ZP_05430877.1" /db_xref="GI:256005933" /db_xref="InterPro:IPR000241" /db_xref="InterPro:IPR002052" /db_xref="InterPro:IPR004114" /translation="MKEIELIATAAAGVESVVKHEVKKLGFKDITVDNGKIMFKGDIS SIPRANLWLRSADRVLLKMGEFEALTFEELFDKTYALPWDQWITSDGKFTVLGKSVKS KLFSISDCQAIVKKAVVEKLKSKYHVEWFEESGPEYTIQVALHKDIATLTIDTSGTAL HKRGYRAKNVEAPIKETLASAMILISYWNKRKPLWDCFCGSGTIPIEAALIGRNIAPG LNRTFASEEWPAIGKDIWKQERALALRAIDHDIKLKIYGSDINPDAIELAKENACLAG VDDCIEFFVSDFRNVNIKEDYGVIICNPPYGERIGEQQEVETINKDMGKTFSKYDTWS KYIITPFENFERLYGKKADKKRKFYNGNIKVDYYQYFGPKPTNT" misc_feature 6634..7743 /locus_tag="ClothDRAFT_2738" /note="Predicted N6-adenine-specific DNA methylase [DNA replication, recombination, and repair]; Region: COG0116" /db_xref="CDD:30465" misc_feature 7108..7728 /locus_tag="ClothDRAFT_2738" /note="S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy)...; Region: AdoMet_MTases; cl12011" /db_xref="CDD:187159" gene complement(7755..8036) /locus_tag="ClothDRAFT_2739" CDS complement(7755..8036) /locus_tag="ClothDRAFT_2739" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430878.1" /db_xref="GI:256005934" /translation="MCNIGDIFTEILKDEINANDQQKLYNGVKRLIKKYSQEKRGLET LDEFVRVLTGGASLEELLQIAREESQNPTITTEIIVDDSCHKNGEIPAT" gene complement(8195..8398) /locus_tag="ClothDRAFT_2740" CDS complement(8195..8398) /locus_tag="ClothDRAFT_2740" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430879.1" /db_xref="GI:256005935" /translation="MDGYNFTEIGMLVGAAIGGILSVIGFMLWHSTITFSFAFAGIAL GILGGSIMDKKLKKSKRENKEFL" gene 9015..9857 /locus_tag="ClothDRAFT_2741" CDS 9015..9857 /locus_tag="ClothDRAFT_2741" /inference="protein motif:PFAM:PF01841" /note="PFAM: transglutaminase domain protein; Protein of unknown function DUF553; SMART: transglutaminase domain protein; KEGG: sat:SYN_00833 transglutaminase-like superfamily protein" /codon_start=1 /transl_table=11 /product="transglutaminase domain protein" /protein_id="ZP_05430880.1" /db_xref="GI:256005936" /db_xref="InterPro:IPR002931" /db_xref="InterPro:IPR007562" /translation="MKKIVSSITMLILTIAIFTNPGFAQTTTVTEYIMSNGVSLQKIP STVEGKITLAGETVNSLIKILVIKDDIQRWYEVKLVDGKFNEEIWLIDGTGKYQVSVL VHKKDRIYTFGPTVFVENAVEVNRFAVPTLHVESNNEKIIALAKELTKDAKTDREKAQ KIYNWVVKNIKYDYDKYLRQLNKNYDNAYGALNTLETGKGVCYDFSALVAALGRASGL QVKMIKGNFIGTYSNELHAWNEIYISEEGRWINVDSTFGSTLGRNFFDNSDFFKDHQK LEEY" sig_peptide 9015..9089 /locus_tag="ClothDRAFT_2741" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.995 at residue 25" misc_feature 9438..9773 /locus_tag="ClothDRAFT_2741" /note="Transglutaminase-like superfamily; Region: Transglut_core; cl10469" /db_xref="CDD:176834" gene complement(9954..11291) /locus_tag="ClothDRAFT_2742" CDS complement(9954..11291) /locus_tag="ClothDRAFT_2742" /inference="protein motif:PFAM:PF00963" /note="PFAM: cellulosome anchoring protein cohesin region; S-layer domain protein; KEGG: S-layer homology domain containing protein" /codon_start=1 /transl_table=11 /product="cellulosome anchoring protein cohesin region" /protein_id="ZP_05430881.1" /db_xref="GI:256005937" /db_xref="InterPro:IPR001119" /db_xref="InterPro:IPR002102" /translation="MKRIKRILAVLTIFALLATINAFTFVSLAQTNTIEIIIGNVKAR PGDRIEVPVSLKNVPDKGIVSSDFVIEYDSKLFKVIELKAGDIVENPSESFSYNVVEK DEIIAVLYLEETGLGIEAIRTDGVFFTIVMEVSKDVKPGISPIKFESFGATADNDMNE MTPKLVEGKVEIIEASAPEATPTPGSTAGSGAGGGTGSSGSGQPSATPTATEKPSTTP KTTEQPHEDIPQSGGTGEHAPFLKGYPGGLFKPENNITRAEAAVIFAKLLGADENSAG KNSSITFKDLKDSHWAAWAIKYVTEQNLFGGYPDGTFMPDKSITRAEFATVTYKFLEK LGKIEQGTDVKTQLKDIEGHWAQKYIETLVAKGYIKGYPDETFRPQASIKRAESVALI NRSLERGPLNGAVLEFTDVPVNYWAYKDIAEGVIYHSYKIDENGQEVMVEKLD" sig_peptide complement(11202..11291) /locus_tag="ClothDRAFT_2742" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.991 at residue 30" misc_feature complement(10782..11192) /locus_tag="ClothDRAFT_2742" /note="Type I cohesin domain, interaction partner of dockerin; Region: Type_I_cohesin_like; cd08548" /db_xref="CDD:176100" misc_feature complement(order(10812..10814,10818..10820,10830..10832, 10953..10961,10971..10973,10998..11000,11004..11006, 11091..11096)) /locus_tag="ClothDRAFT_2742" /note="dockerin binding interface; other site" /db_xref="CDD:176100" misc_feature complement(10509..>10574) /locus_tag="ClothDRAFT_2742" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" misc_feature complement(10317..10448) /locus_tag="ClothDRAFT_2742" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" misc_feature complement(10131..10253) /locus_tag="ClothDRAFT_2742" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" gene complement(11344..13410) /locus_tag="ClothDRAFT_2743" CDS complement(11344..13410) /locus_tag="ClothDRAFT_2743" /inference="protein motif:PFAM:PF00963" /note="PFAM: cellulosome anchoring protein cohesin region; S-layer domain protein; KEGG: S-layer homology domain containing protein" /codon_start=1 /transl_table=11 /product="cellulosome anchoring protein cohesin region" /protein_id="ZP_05430882.1" /db_xref="GI:256005938" /db_xref="InterPro:IPR001119" /db_xref="InterPro:IPR002102" /translation="MKKNNVLTIAAMIALLLTSLLTSITFGETSSIPSRISMELDKTK ANIGDIIIATIRIDNINNFSGYQLNIKYDPSYLQAVNPLTGEPIKKRTMPAVNGTVLL KGDQYSITEVVENNVDEGILNFGKGYANLTEYRKSGKPETTGIIGKIGFKALKLGKTE IKFENTPVMPGAKEGTLLFDWDAETITEYNVIQPKELAITLPDDAHIALELDKTKVKV GDVIVATVKAKNMTSMAGIQVNIKYDPEVLQAIDPATGKPFTKETLLVDPELLSNREY NPLLTAVNDINSGIINYASCYVYWDSYRESGVSESTGIIGKVGFKVLKAANTTVKLEE TRFTPNSIDGTLVIDWYGQQIVGYKVIQPDKITVISEPEVPTQTPTQTPPTTTAPSQT PTQTPPTTTAPSQTPTQTPAVTPTQSATPSDPGGGGGGLPGGGGGAVNPSASPTPTPT SKPTPTATKKPEPTEIEEPEPEIPGTVGIHYSYLTGYPDKMFRPEKSITRAEAAVIFA KLLGANENTKINYNVSYTDVDSSHWASWAIKFVSYKKLFTGYPDGSFKPNQNITRAEF STVVFKLLVSEKGLKEEKIEKSKFGDTKGHWAQQFIEQLSDLGYINGYPDGTFKPNNN IKRSESVALINRAMGRGPLHGAPQVFEDVPQTHWAFKDIAEGVLNHRYKLDNEGKEQL LEIIDN" sig_peptide complement(13327..13410) /locus_tag="ClothDRAFT_2743" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.972 at residue 28" misc_feature complement(12829..13311) /locus_tag="ClothDRAFT_2743" /note="Type II cohesin domain, interaction partner of dockerin; Region: Type_II_cohesin; cd08547" /db_xref="CDD:176099" misc_feature complement(order(12856..12858,12862..12864,12874..12876, 13024..13032,13042..13044,13069..13071,13075..13077, 13210..13215)) /locus_tag="ClothDRAFT_2743" /note="dockerin binding interface; other site" /db_xref="CDD:176099" misc_feature complement(12319..12798) /locus_tag="ClothDRAFT_2743" /note="Type II cohesin domain, interaction partner of dockerin; Region: Type_II_cohesin; cd08547" /db_xref="CDD:176099" misc_feature complement(order(12349..12351,12355..12357,12367..12369, 12517..12525,12535..12537,12592..12594,12598..12600, 12697..12702)) /locus_tag="ClothDRAFT_2743" /note="dockerin binding interface; other site" /db_xref="CDD:176099" misc_feature complement(11899..>11964) /locus_tag="ClothDRAFT_2743" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" misc_feature complement(11713..11841) /locus_tag="ClothDRAFT_2743" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" misc_feature complement(11524..11646) /locus_tag="ClothDRAFT_2743" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" gene complement(14083..>15065) /locus_tag="ClothDRAFT_2744" CDS complement(14083..>15065) /locus_tag="ClothDRAFT_2744" /inference="protein motif:PFAM:PF00395" /note="PFAM: S-layer domain protein; KEGG: bgl:bglu_2g01500 rhs element Vgr protein" /codon_start=3 /transl_table=11 /product="S-layer domain protein" /protein_id="ZP_05430883.1" /db_xref="GI:256005939" /db_xref="InterPro:IPR001119" /translation="PSDEPTPSDEPTPSETPEEPTPSDEPTPSDEPTPSETPEEPTPT TTPTPTPSTTPTSGSGGSGGSGGGGGGGGGTVPTSPTPTPTSKPTSTPAPTEIEEPTP SDVPGAIGGEHRAYLRGYPDGSFRPERNITRAEAAVIFAKLLGADESYGAQSASPYSD LADTHWAAWAIKFATSQGLFKGYPDGTFKPDQNITRAEFATVVLHFLTKVKGQEIMSK LATIDISNPKFDDCVGHWAQEFIEKLTSLGYISGYPDGTFKPQNYIKRSESVALINRA LERGPLNGAPKLFPDVNESYWAFGDIMDGALDHSYIIEDEKEKFVKLLED" misc_feature complement(14653..>14718) /locus_tag="ClothDRAFT_2744" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" misc_feature complement(14464..14595) /locus_tag="ClothDRAFT_2744" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" misc_feature complement(14260..14382) /locus_tag="ClothDRAFT_2744" /note="S-layer homology domain; Region: SLH; pfam00395" /db_xref="CDD:144111" ORIGIN 1 tctccgactc agaaaaaaat aagacattaa atgtcttatt tttttgccgg attgctttca 61 tccatgtatc acgctgatat caacggttta tggtgcttta gaaccaattt gttttcagac 121 ttctttcttt tcaaaatact cgttcagctc tttaacaagc ttatcagcgt ctatcccgtg 181 aaccgcacag gcatcttcaa tgctttctcc cattgaagac ggacatccaa ggcaatgcat 241 tccgttgttg atgaatatgg gagcagtacc tctgtccatt tgcagtacat cggctataat 301 catatccttt gtaatcttca cttccatccc tccaaatctt ttaaatgtta aacatcgtaa 361 aaatttaacc taaattttat tatacactat tgtcaaataa aaatataccc tgttttttcc 421 ccgttttatt taatattttt ataggctaac attttttatg ggctaacaat ccggtatttt 481 tctcttccga ttttataaag atcgtttcca tagctgtcga ttatcacaac tgccggaaaa 541 tctttgacag taagctttct tatcgcctct gtgccaagtt cgggaaaagc tatcaattcc 601 tccgcaacaa ttgactttgc aatcagggct cccgcgcctc cgattgctcc gaaatatacc 661 gcaccataac ttttcattgc atccacaacc gcttcacttc tcagtccctt gcctatcatt 721 cccttaagtc cgagttttat cagcaaagga gcataagcat ccatcctgct gctggttgtc 781 ggacctgcgg aaccaatgac ttcaccgggt tttgcagggc aaggcccaac ataatatatt 841 atctggtccc ttatatcaaa aggcaacggc ctaccctctt gtataagatt taccattttt 901 ttgtgtgccg catctctggc cgtgtatatt gttccattga tacttacaac atctcctgcc 961 ctaagttctc tggccttttc cccggtcaaa ggcgcttcaa taatgtactt catatttacc 1021 acccttcaca taataactaa catttaaata attatttctg catgtcttgt tgcatgacag 1081 tttatattca cggccaccgg aagacctgca atatgggttg gaaaaacttc aatatttaca 1141 cctaatgccg taattcttcc tcccagtccc gaaggaccta tgcccagcct gtttatacgc 1201 tcaagcattt cctcttccag ctcctttaaa tacggtatgt cattcctttt gtctatcggc 1261 ctcaaaagcg cttttttggc caaaaaagcc gccttttcca tggttcctcc gatacctacg 1321 ccgacgacaa taggaggaca gggattcggc cccgcttttt ccaccgtctc aattataaaa 1381 ttcttaactc cttctattcc ctgggaaggg gtaagcattt tcagggcact catattttca 1441 cttccaaaac ctttaggagc tacagttatt tttattttgt ctccgtcaac aatgttgtaa 1501 tgaatcaccg ccggagtatt atcctttgtg tttatccttt ctatagggtc atttacaacc 1561 gactttctga gatagccctt ctcatagccc cttctaaccc cttcattgat tgcatcagtg 1621 aggttacctc ccgtgatatg tacatcctgg cctatgtcca taaaaacaac ggccattccc 1681 gtatcctgac agattgccac ggccttttcc cttgctatct gcgcattctc aataatcttg 1741 cttaaaatct ctttgccatt gtccgattct tctttttcaa gccctttttc caagccattt 1801 attatatcat cgttaagata ataatttgag tccatgcaaa gcttttccac agcttcagta 1861 atactatcaa catgaattgt tctcatgaca tcccccgacc tcttgtttct taaacttgcc 1921 gcctaaaact tttaaagttt aattctagta tacaaaaacc tgttttgact gtaaacagga 1981 ataaagataa atcaaacatt ttttgataat taaataggac tttttttaat atctctgata 2041 taataaaagt agagggcgat tgaaaaattg cctgccaaat cttttgggga ttaagcattg 2101 ctaattttta agcatatatt gcaatataaa tgttttactg acaataggag gggttataag 2161 ccttaattct ttgcatccgt ttgccgataa gcatcttatg aaaggggttt ttatcttgag 2221 ttcacctcag aacattttag tttgtgtaac tcagcagatt acttgcgaaa gacttatact 2281 aaaagccgca gaattaagaa acgaattaaa gggcgaactc tttgtcatac atgttgcaaa 2341 aaatgaatgg aacttcctgg acaacataaa ggagggagag gctttagaat acttgtttaa 2401 aatatcaaaa tcggtcggtg caaacctttc agtgttaaaa tccgatgata ttgttcaaac 2461 catagtgaat tttgcaaagg aaaataaaat tacccatatt gtaatgggag agtcccctaa 2521 tgaccacaag gaaaacaatt tttacaatga actgaagaga cttttaagta atgttgagat 2581 tcttgtcata ccccaggccg gaatctgatt acaagctttc cggcatgttc tcagcaaatt 2641 tagtgatata ttatacacca ttatttataa ttatgcaatt aataattgat tttttacaaa 2701 aaaatttgat ttatgtcatt aatttattcg gaattgcttg actttccgcg attttcatta 2761 taaaatattg tcgaattcca aatttaggag gttgtgtaca tgaacaaaac tgaattaata 2821 aactcaattg cttcgaaatc cggtctgagc aaaaaaaaca gtgaagctgc tctgaacgct 2881 tttatcgcat ctgttgaaga agcacttaag aaaggtgaca aagtagtact cgttggtttc 2941 ggcactttcg aagttaagaa cagagctgca agaaaaggaa gaaatcctca aacaaaagaa 3001 gaaatcacaa tccccgcatc caaagctcct gtatttaaag caggaaaagg attaaaggac 3061 atagttaaca aataattaaa ttatatcatt aaaagacctg tcgtacgaca ggtcttttag 3121 attaatcagg caatttaagg cctaagcccc atggttattc ttattttatc agccatcatt 3181 gctatacact cggaattggt gggttttcct ttgctgtaat taatcgtgta tccaaacaac 3241 gcgtccacag tatcgggatt tccccttgcc caggcgctct ctatggcatt tctgatcgcc 3301 cgttcaactt tttgaggtgt ggtattgtac ttttcagcaa cggcagggta taaattcttt 3361 gtgacggacg taaagacttt tgaattcttt actgtttgta tgacggcctc acgaagatac 3421 tgataaccgg ccatatgggg aggtataccc acctcatgca tcaaattggt tacttcgact 3481 tcaagatcat agtttttgtc aacgttgctg cttaaaagct cggaattctt gataatcggc 3541 ttgtttgaga aaacggacgt tgtcttttcc ctgtacagct gtcttattct cgtgaccagc 3601 acttccacat caaaaggctt gattatatag tattccgcac cgagattaac agctctttgc 3661 acaaacacat cctgtccgat tgctgaaagc attatgaaga tgggcctgta ttcaagctgc 3721 atggttgaca gcctttccag aacccccaaa ccgtccaggt tgggcataat aatatcgagc 3781 acaactacat cgggcttttt ggaaataatc atttctattg cctgtaaacc gtcctttgca 3841 agttccaaaa cttctatgtc ttcgtattga ttcatgtatt ccttcaacag ttttgcaaac 3901 tcaacattat catcggcaat taaaactgaa atttctccct tcattccatt accccacttt 3961 aaatgaaata attctaaata caaaatgttc taaaatgtgt catataattt attattaatc 4021 gctgaaacaa ttccttctat tttacattat aatataaaac tttataaaat aatactatat 4081 ttttgaagtt gtcattcaca agtggatata tccgcaccgc accgggacaa aaaatttaca 4141 aaatccggga aagtaacatt gaccgcctcc gcagtgtcaa taacggtctc accttctgca 4201 ttcaagcctg ctatagcgag cgacataacc acacgatggt ctccgtaacc ttccagttta 4261 catcctttca atctgctttc ccttatcaca agtccatctt ccagctccgt tatatccgca 4321 cccatcttct ttaactgctc gcacataaca tgaatcctgt cggtttcctt aatccttgcc 4381 tgaggcacat ttaaaagttt agtctcacct tttgcaaaac atcctgcaac agccatggcc 4441 ggaagagcat caggtatcgc attcatgtct atctcccttc ccacaagttc acaacctttt 4501 attctgacac tctttccttc cacttttacc ttggctccca tatcttcaag tatggaaaac 4561 accatcttgt ctccttgggg atccgtcata tccaggttgt ccagcacgaa ctcctctccc 4621 gatattgcag cctggacggc aaagaaggtt gccgaagaaa aatcccctgg tatcgtaacg 4681 tttaaaggcc tgtaacgctg gccgcccttt atgtaaaaag ttttgtaatc gtgattttca 4741 tactttattc ccagtttatc aagccaccac aacgtcatgt ccacataagg aacttcgttg 4801 agccttgtaa caatcacctc ggtatccaat ggaataagag gtgaattaat cagtattgac 4861 gaaagatact gggatgtaac cgaatcaatc tctgtccttc cgcctttaag ctttcccttc 4921 accaccacag gcgctcttcc gttgtttctg gtggaaaaag cctccgcccc aaggttgttt 4981 atggcgcaaa gcaaaggtcc aagcggtctt tgccttatct gccggtctcc ggtaaatacc 5041 gaacatccgt cacccaaagc agccgtcata attccaaatc gaagtgtggt acccgaattg 5101 ccgacgttta ttacatcctc gggaacttcc ggatttcctc caaatccttc gacaacatat 5161 ttatcgtcct ctttttcaat tttagccccc aaggctctgc aaacctccac cgccgacaac 5221 gcatcatccg atatcagagg gctttggatt tcggattttc cctcggcaag gcttgcgaaa 5281 aacaatgccc ttatggtatg ggattttgaa cctggaatcc tcacatttcc actggctttt 5341 gattttctaa cctttaacag catattgttt accctttcta ccgtactgca agttttttta 5401 aaacagtgta ttacaattat attttaatat aaaataaaaa tataataaac atcattcatc 5461 aagtttttcg ttaaaaacaa aaattcgtca aaacaataaa ttgaactatc tgttatttgt 5521 tgaaaaattg tgttaaaatt aatttgaaaa ctaaacgaaa gcaagggtat aagataatgg 5581 acggtactgt attgcttatt gattatttcg aatatgagag ggaaaaaacc aaaatcatat 5641 tcgacaatat cggagaatat gattttatag aagttgaaaa cctgaaaaaa ttttacagta 5701 tttttaaaga ccttgattcc ataaccctga taattatgga tatagctttc cctgtcgaaa 5761 aagaaggtct tgaggttctg tcggcaatca gaaacaattc caggactgcc aacacgccgg 5821 ttattattgc caccaaatcc gacaatccgg gctatcgtca tgcggcgctc aagttcaaag 5881 taagtgatta catcctaaag ccgtacccga ccaaacgcct tgagaattca gtaagaagcg 5941 tgttaaaaat atgccagcgt ttcagatatg aatttgacag cgcccatgtt atctccatgt 6001 ctattgagga ctacatatcc aaggaattta aagtagcttc aagagcaggc cagagcctgt 6061 ccgtaattct tatggctccg gtcggcctgg aaaaagaacc gtcggaacag tccactcaaa 6121 tttcttctga gcttcaggac caaattcaaa acctggcaat cgaaaaagta aagctttcat 6181 tgcgctccac agacacggca attctcaatg ccaacaggga cattcttgtc atattgcctt 6241 tcaccaacgc cgccggtgcg caaaaagtcc ttcagaaaat ccaggacaac gtaagagaag 6301 gccttaaaag cctgaatata aattatgacg attattacta cgccgtctcc gttacctttc 6361 ccaatgacgg caaaaccttt cagagtctta tggaaaaggc ggtaaagaag gttgaggaca 6421 agattatgct ggaaaaaata acttccatag gcgtaaatat tcttgacaac gccagaaatt 6481 catacaaaaa atttaacaag taagtatgca ttctttcatt tgttatcggt aatcctaagc 6541 ttggttcagg ttttgttttg tgctttttat tatatagaat ttattgtaat ttttgtaaat 6601 ttatatatca atggaggaaa tatgaaagaa attgaactta tagcaacagc ggcagccggt 6661 gtggaatctg tggtaaaaca tgaagtaaaa aaactgggtt ttaaagatat aaccgtagat 6721 aacggcaaaa taatgtttaa aggggatata tcaagtattc cccgggcaaa tttatggtta 6781 agatccgccg acagggtatt gcttaagatg ggtgaatttg aagctttaac ttttgaagag 6841 cttttcgata aaacctacgc cctcccctgg gatcagtgga taaccagcga cggtaaattc 6901 accgtattgg gtaaatcggt aaagtcaaaa cttttcagca tctccgattg tcaggcaatt 6961 gtgaaaaaag cagtggtaga aaagctaaag tccaaatatc atgtggaatg gtttgaagaa 7021 agcggccccg agtataccat acaagtggcc cttcacaagg acattgcaac tttgaccatt 7081 gatacaagcg gtacagcact gcacaaaaga ggctacaggg ccaaaaatgt tgaggccccc 7141 ataaaggaaa ctcttgcatc cgcaatgatt ttgattagct attggaacaa aagaaagcct 7201 ctgtgggatt gcttttgcgg ttcaggaaca attccaattg aagctgctct catcggacgc 7261 aacattgctc cgggtcttaa cagaactttc gcctctgaag aatggccggc tataggcaag 7321 gatatatgga aacaggaaag agctttggcc ttaagggcaa tagaccacga tattaaactt 7381 aaaatctacg gttcggatat aaatcccgac gctattgaac tggcaaaaga aaacgcctgt 7441 ctcgcaggag tcgatgattg tattgaattc ttcgtcagtg atttcagaaa tgttaacata 7501 aaggaggatt acggcgtaat catatgcaat cctccctatg gcgaaagaat aggtgaacaa 7561 caagaagttg aaacaataaa caaggatatg gggaaaacat tctccaaata tgatacctgg 7621 tcaaaataca taattacccc gtttgaaaac tttgaacgtc tttacggtaa aaaagccgat 7681 aaaaagcgca aattttacaa tggaaacata aaagtggatt attaccaata cttcggtcca 7741 aaaccaacca acacctaagt ggctggaatt tctccgtttt tgtggcagct gtcatccaca 7801 ataatttctg tagtaatagt tggattttgt gattcctctc tggcaatttg cagcagttcc 7861 tccaacgaag ctcctccggt taaaaccctt acaaactcat cgagggtttc caaccctctt 7921 ttctcctggg aatacttttt tataagcctt tttactccat tataaagttt ttgctggtca 7981 ttggcattga tttcatcttt caggatttcc gtaaatatat ctcctatgtt gcacatttct 8041 ttttcaagac tttctatctc ccctaaaagc cttaaaattt tattgccgtt ttgactgctt 8101 atggtatgcc actcctttat ataaaattat atatttatag tatcttcgat aaagaaagat 8161 ttattccgcc aatgacattc aattgagttt cctgctataa aaattcctta ttttcccgtt 8221 tactcttttt taatttttta tccatgatgc ttccgcccaa aatacccaaa gctatccctg 8281 caaatgcaaa agaaaaggta atcgtgctgt gccaaagcat aaaacctatt accgaaagta 8341 tgcctcctat agccgctcct acaagcatac cgatttcggt aaagttatat ccgtccatcc 8401 ctgtattccc tccgaatact ccgtacagtc ttccatacaa ttttcttgat aaatacttcg 8461 atattccaat tttttggctt tatatttatg taaattagac gatatattaa ttagacgatg 8521 ttcttaaaga aaagttctga gttttcataa aaaagccaaa aaaatctcca taattttata 8581 aaaagtgatt gaaattccac tagttttgag gtataatata attgtagaca agtaaatatt 8641 gtacttttga aaagggcaaa tctatcgaaa gatagagacg caaagccaac gagtctaagg 8701 tggaaacact atgacggtcg ggttgccaaa agtaaaagtt gtaagatcat tttgatctta 8761 aaggtcactt tggtcttaat agctctgttt ggtataccca gacagagctt taatttttct 8821 taacacctgc tttcgtgtcg acttaaattt actcgcttca ttgttacatt ttcacaactg 8881 aataccaaga taacatcctt agccctttcg acaaaggcaa actcaatgaa aattgaggac 8941 gcaaagctcc aggtcttcgg tttggtattc caaaccatga cggctgagtt accgaaaaag 9001 ggaggaattg atttatgaaa aaaatagtaa gcagtattac tatgctcatc ttaactattg 9061 ccatttttac aaatccggga tttgcacaaa ctaccaccgt cacagagtac ataatgtcca 9121 atggagtctc tctccaaaaa atcccgtcaa ctgttgaagg aaaaattaca ttagccggtg 9181 aaactgttaa cagcttaatc aaaatacttg tcatcaagga tgatattcag cgctggtatg 9241 aggttaagct tgttgacggc aaatttaatg aggaaatttg gcttattgac ggaacaggca 9301 aataccaggt ttccgttttg gttcacaaga aagatagaat atataccttc ggaccgacgg 9361 tgtttgttga aaatgccgtg gaggtaaaca ggtttgcagt acccacactt catgttgaaa 9421 gcaataatga aaaaataatt gctttggcaa aggaattaac caaagacgcc aaaactgaca 9481 gggaaaaagc ccaaaaaatc tataattggg ttgtcaaaaa tattaaatat gattacgata 9541 aatatttaag acagcttaat aagaactatg acaatgcata tggtgctctt aacactttgg 9601 aaaccggaaa aggcgtatgc tacgatttct ctgcactcgt tgctgccttg ggcagggcat 9661 caggattgca ggtaaaaatg attaaaggaa atttcattgg gacatacagc aacgaattgc 9721 acgcctggaa tgaaatatat atctccgaag aaggcagatg gattaatgtt gactccacct 9781 ttggttcaac tcttggaaga aacttctttg acaacagcga ttttttcaag gatcatcaga 9841 aacttgaaga atattaagca tacctccata taaaacaaac ccaacaagtt ttaaggctca 9901 gaaagccaac gctttctgag cctctttttt atctacggcg catttacaaa ctgttaatca 9961 agcttttcaa ccattacttc ctgtccgttt tcatcaattt tataactgtg ataaattaca 10021 ccctcagcta tatccttgta tgcccaatag tttacaggaa catccgtaaa ctcaagaact 10081 gcaccgttca gcggacctct ttcaagggat ctgttaatga gagctacaga ttccgccctc 10141 ttaatacttg cctgaggtct gaaagtttca tcaggatagc cttttatata tccttttgca 10201 accaaagtct caatatactt ttgagcccag tgtccttcta tgtcttttaa ctgagtcttg 10261 acatcggttc cctgttcgat ttttccaagt ttctcaagga atttgtaagt aacggttgca 10321 aattcagccc ttgttatgct cttgtccggc ataaaagttc cgtcgggata gccgccaaag 10381 agattttgct ccgtaacata ttttatagcc catgccgccc agtggctgtc ttttaaatcc 10441 ttaaaagtga tggatgaatt tttgcctgcg ctgttttcat ccgcacctaa aagtttggca 10501 aagataactg ccgcttccgc ccttgtaata ttgttctcag gcttgaacag tccccccgga 10561 tatcctttaa ggaacggtgc atgctcgcct gtaccaccgc tctgaggtat gtcttcatgc 10621 ggctgctcag ttgtctttgg agtagttgac ggtttttccg ttgccgttgg cgttgctgac 10681 ggctgtccgg aaccggaaga acccgtaccg ccacctgcac ccgatccggc cgttgaaccc 10741 ggtgtcggag ttgcctccgg agcggatgct tcaataattt ccactttacc ttccacaagt 10801 tttggggtca tttcgttcat atcattatct gcagtagccc caaagctttc aaattttatc 10861 gggctaatcc ccggctttac atctttgctt acttccatca ctattgtaaa gaatactccg 10921 tcggttctta tggcctcgat acccaaaccg gtttcttcca aatacaaaac ggcaataatt 10981 tcgtccttct ccactacatt gtaactaaag ctttctgaag gattttccac aatgtctccg 11041 gcctttaatt ctattacttt aaagagtttt gagtcatatt caattacgaa gtctgaactg 11101 actattcctt tgtcaggaac atttttcagg cttaccggca cctcaattct gtcacccggt 11161 cgtgctttga cattacctat aattatttca atggtgtttg tttgtgccag tgaaacaaac 11221 gtgaatgcat taatagttgc aagcaaagcg aaaattgtaa gcactgctaa aattcttttt 11281 attctcttca tacttatcct cccaaaataa tttaattaat atgatatgaa cacatcaatt 11341 tttttagtta tcaattatct ccagcaattg ttctttgccc tcattgtcca gtttgtatct 11401 gtgattgagc acgccctctg caatatcttt gaaagcccag tgtgtctgag gaacatcctc 11461 gaatacctgc ggtgcgccat gcaaaggccc tcttcccata gctctgttta tcagggcaac 11521 actttctgat cgtttgatat tgttgttggg cttgaatgta ccatcaggat atccgttgat 11581 gtatccaagg tctgacagct gttcaataaa ctgttgtgcc cagtggccct ttgtatcacc 11641 aaacttggac ttttcaatct tttcttcttt tagacctttc tcagatacaa gaagcttaaa 11701 cacaaccgtt gaaaattcgg ctctcgttat attctgatta ggcttgaacg agccatcagg 11761 atatccggta aacagtttct tgtatgatac aaatttgatt gcccaacttg cccaatggga 11821 gctgtcaaca tcggtgtatg aaacattata gtttatcttt gtattttcgt ttgctcccaa 11881 aagttttgca aaaatcacgg ctgcttcagc tcttgtaata ctcttttcag gtctgaacat 11941 tttgtccgga taacctgtca ggtatgaata atgtattcca acagtgcccg gtatttcagg 12001 ttcgggttct tctatttccg ttggctccgg ttttttagtg gcagtaggag taggtttgga 12061 tgtcggtgtt ggtgtcggtg aagctgaagg attaacagcg ccgcctccac cacccgggag 12121 gcctcctcca cctccgccag gatccgacgg agttgcactt tgcgtcggcg ttactgccgg 12181 tgtctgtgta ggtgtctgtg atggtgctgt tgttgttggc ggtgtctgcg taggtgtttg 12241 cgatggtgct gttgttgttg gcggtgtctg tgtaggtgtt tgtgttggta cctcaggctc 12301 tgaaatcaca gtaattttgt cgggctgtat tactttataa ccaactatct gttggccata 12361 ccaatcaatt accaaagtac cgtctatcga atttggtgta aatcttgttt cttccagttt 12421 tactgtggtg ttggcagctt tcagcacttt aaagccaacc tttccaatta ttccggtgct 12481 ttcagatact cctgattctc tgtaggaatc ccaatataca taacaagatg cataatttat 12541 aatgccggaa tttatgtcat taactgctgt taacaacgga ttatattctc tgtttgataa 12601 cagttccggg tccacaagta atgtttcttt tgtaaacggt tttcccgtcg caggatcaat 12661 cgcctgcaat acttcagggt catatttaat atttacctga attcccgcca tactagtcat 12721 attctttgct tttactgtcg caacaattac atctcccact ttcacttttg tcttgtcaag 12781 ttccaaagca atgtgtgcat cgtccggtaa cgttattgca agttctttag gctgaattac 12841 attatattcc gttatagttt ctgcatccca gtcaaacagc agtgttcctt cttttgcccc 12901 aggcatgacg ggtgtgttct caaatttgat ctccgtcttg ccaagcttta aggctttaaa 12961 tcctatcttg ccaataattc cggttgtttc aggttttccg cttttcctgt attcagttaa 13021 atttgcatat cccttgccaa aatttaaaat cccttcatcg acgttatttt ctacaacctc 13081 agtaatactg tactgatctc cctttaacaa caccgtgccg ttcactgccg gcattgttct 13141 cttttttatc ggttctcctg tcaaaggatt aactgcctgg aggtatgacg gatcatactt 13201 tatgtttaat tgatatccgc taaagttatt gatattgtca attcttattg tggctataat 13261 tatgtcgcct atgtttgctt ttgtcttgtc aagctccata gatattcttg aaggtatact 13321 cgaagtctcc ccaaaagtta tacttgtaag taagctggtt agaagaagcg ctatcatagc 13381 tgctattgtt aatacattgt tttttttcat aaaacccccc caaatatatc atataatagt 13441 tttatttgtt gtccatttat ttttatgttt atgtatccat ttaacacccc caatattaat 13501 tttttatgtt atttattgtt ttttataaaa aacagcaaac tttattgtta tcacgctcaa 13561 aaacagttgc taacactatt gtcgtattta atctttaaaa cagcaaaact accttagggt 13621 tatgttatcc caattacacc ttttaattaa tctaccgtta tacttacagc gaacgttttg 13681 aatttttcaa ttggagtgaa tagactgtgg caatagcggg gataagagct ggtacattac 13741 aaacgtctct gaatgatttt tcaagcgtta tctttttaaa caagaaagga tatgattttt 13801 aaaaatgaca caccacaata attcctcccc agaaaaataa atcgattaca gtaaccactg 13861 gaacatattt tcagtctcta taagcacttg aattggatat ccgttatatt tttattataa 13921 attagttttt tcaatgtttc aagtcatttt gcgaataatc tgttgttttt caggaaatct 13981 gaatcttgtt tgctttttac tttcaaatat aaaaaagctg ctgcgtcaaa gaactaaacg 14041 caacagcttt tgacatatca tttcaattta tcttacacaa cattaatctt cgagcaattt 14101 aacgaatttt tctttctcat cttcgataat gtaactgtgg tcgagagcac cgtccataat 14161 gtcgccaaat gcccagtatg attcgttaac atccgggaag agcttcggcg ctccattaag 14221 cggacctctc tccagagctc tgttaatcag tgccacactt tcggaacgtt taatatagtt 14281 ttgcggcttg aacgttccgt caggatagcc actaatataa cccaagcttg tcaatttctc 14341 aataaactct tgtgcccaat gtccgacaca atcgtcaaac ttcggattac ttatatctat 14401 tgttgcaagc ttgctcatta tttcctgacc cttaactttt gtcaggaagt ggagtaccac 14461 agttgcgaat tccgctctcg ttatgttctg atcaggttta aacgtaccgt ccggatatcc 14521 tttgaacaag ccctggcttg ttgcaaattt gattgcccat gcagcccagt gagtatcagc 14581 caaatcacta tatggacttg cagactgagc tccatagctt tcatcggctc caagcaactt 14641 agcaaagatt accgccgctt cagctcttgt tatatttctt tcaggcctga agcttccatc 14701 cggatatcct cttaagtatg ctctatgttc tccaccgatt gcaccaggca catcagatgg 14761 tgtaggctct tcgatttctg tcggtgcagg tgtagacgtc ggtttagatg tcggtgtcgg 14821 tgttggagat gtaggtacag ttcctccacc acctccgccg ccaccaccgc ttccaccact 14881 gcctccgctg ccacttgtag gcgttgtcga cggtgttggt gtcggtgtag tagtcggtgt 14941 cggctcctca ggtgtctctg atggagtcgg ttcatctgac ggtgttggct catcagacgg 15001 tgtcggctcc tcaggtgtct ctgacggagt cggttcatct gacggtgttg gctcatcaga 15061 cggtg //