LOCUS NZ_ACVX01000074 2827 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00078, whole genome shotgun sequence. ACCESSION NZ_ACVX01000074 NZ_ACVX00000000 VERSION NZ_ACVX01000074.1 GI:256006153 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000074. FEATURES Location/Qualifiers source 1..2827 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene <1..292 /locus_tag="ClothDRAFT_2936" CDS <1..292 /locus_tag="ClothDRAFT_2936" /inference="similar to AA sequence:KEGG:Pcar_1355" /note="KEGG: pca:Pcar_1355 putative methyl-accepting chemotaxis protein (MCP)" /codon_start=2 /transl_table=11 /product="putative methyl-accepting chemotaxis protein (MCP)" /protein_id="ZP_05431073.1" /db_xref="GI:256006154" /translation="EESAAASEELSSQAEILRESVAKFKLKNMGKVSYSKYKELSPEI MRMLEDYTERKQPKSLNRQEDIKYTDDEERTENDVGNLKQKILLSDSEFGKY" gene 1064..1528 /locus_tag="ClothDRAFT_2937" CDS 1064..1528 /locus_tag="ClothDRAFT_2937" /inference="protein motif:PFAM:PF01584" /note="PFAM: CheW domain protein; SMART: CheW domain protein; KEGG: pca:Pcar_0995 chemotaxis signal transduction protein, purine binding protein, CheW-like" /codon_start=1 /transl_table=11 /product="CheW protein" /protein_id="ZP_05431074.1" /db_xref="GI:256006155" /db_xref="InterPro:IPR002545" /translation="MTETLVNELELQQEETQRNKYLTFVVEKEYYGIEIRYVTEIIGI QSITEVPDLPEYIRGIINLRGKIIPVMDVRLRFGKKPREYDDRTCIIVIDINDMFVGL IVDSVCEVMSIPDGEIVVPPDFDQNENRFIKGIGKTGNEVRLILDCEKLLKD" misc_feature 1121..1525 /locus_tag="ClothDRAFT_2937" /note="CheW, a small regulator protein, unique to the chemotaxis signalling in prokaryotes and archea. CheW interacts with the histidine kinase CheA, most likely with the related regulatory domain of CheA. CheW is proposed to form signalling arrays together...; Region: CheW; cd00732" /db_xref="CDD:29682" misc_feature order(1247..1264,1346..1348,1352..1354,1508..1525) /locus_tag="ClothDRAFT_2937" /note="putative CheA interaction surface; other site" /db_xref="CDD:29682" gene 1581..>2827 /locus_tag="ClothDRAFT_2938" CDS 1581..>2827 /locus_tag="ClothDRAFT_2938" /inference="protein motif:PFAM:PF00672" /note="PFAM: histidine kinase HAMP region domain protein; SMART: histidine kinase HAMP region domain protein; KEGG: acp:A2cp1_2573 methyl-accepting chemotaxis sensory transducer" /codon_start=1 /transl_table=11 /product="methyl-accepting chemotaxis sensory transducer" /protein_id="ZP_05431075.1" /db_xref="GI:256006156" /db_xref="InterPro:IPR003660" /db_xref="InterPro:IPR004089" /db_xref="InterPro:IPR004090" /translation="MRWYLNLKISAKLIIAFLLLAAIAALVGGVALVNINNMSREDVF LYENNTLGISYAADAALQFQRMRFNGARMLLFDEVEKQNESIGKIEEHIVNVEKYLSL YEAAITNETDRQLFEDLKPKWEKYKSVITKETEYVKAGQLEEAKTLLLGDMADAGDTL RDAFEDLMEYNTTAAKEKVDENKQVASTASTIMITVIFAGILIAIALGVFISRIISRP IAQMVEAADRLALGDVEVDVKAETRDEIGKLAESFKRMIENIREQAYVVERIAAGDMT VDVRVRSDKDLLGKKLKEMVDTNNEVLSNINEVAAQVAAGAKQVSDSSMQLSQGATEQ ASSIEELTASLEQVANQTQLSAKNANQANELAEVAKNNAEQGNKQMAEMLNAMEEINN SSSNISRIIKVIDEIAFQTNILAL" sig_peptide 1581..1682 /locus_tag="ClothDRAFT_2938" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.800 at residue 34" misc_feature 2223..2366 /locus_tag="ClothDRAFT_2938" /note="Methyl-accepting protein, and Phosphatase (HAMP) domain. HAMP is a signaling domain which occurs in a wide variety of signaling proteins, many of which are bacterial. The HAMP domain consists of two alpha helices connected by an extended linker. The...; Region: HAMP; cd06225" /db_xref="CDD:100122" misc_feature order(2223..2228,2235..2240,2244..2249,2256..2261, 2265..2267,2313..2318,2322..2327,2334..2339,2343..2348, 2355..2360) /locus_tag="ClothDRAFT_2938" /note="dimerization interface; other site" /db_xref="CDD:100122" misc_feature <2343..>2827 /locus_tag="ClothDRAFT_2938" /note="methyl-accepting chemotaxis protein II; Provisional; Region: PRK15048" /db_xref="CDD:185008" misc_feature 2658..>2827 /locus_tag="ClothDRAFT_2938" /note="Taxis toward Aspartate and Related amino acids and Homologs (TarH). The Tar chemoreceptor of Escherichia coli mediates attractant responses to aspartate, maltose, and phenol, repellent responses to Ni2+ and Co2+, and thermoresponses. These...; Region: TarH; cl00144" /db_xref="CDD:153541" ORIGIN 1 agaagaaagt gctgctgcaa gtgaagagct gtcaagtcag gctgagattt tgagagaatc 61 cgttgcaaag tttaaattga aaaatatggg aaaggtatca tacagtaagt ataaggaact 121 tagtcctgaa attatgagga tgcttgagga ttatacggaa aggaaacaac ctaaaagtct 181 caacaggcaa gaagacataa agtacactga tgatgaggag agaacggaaa atgatgttgg 241 taatttaaaa caaaaaatat tgttgtctga cagtgaattt gggaaatatt aaggatgtat 301 aaagttaata cataaagcag agtggacttt agtgttcact ctgttttttt atattgatta 361 tttttaaatt agttttctat gctatagctg ggataagttg gtattgcttt gcagatataa 421 aatttttatt ggacaaagcc gatataaaaa agtgttgaat agtcgcaata tttgtctgta 481 taagaattat atgaaaattt tatgagaatt ttaaaaataa gaaggaagtt tattatttat 541 gtagaataaa tataaatcat ccgataatta taatgtgttt atgtaatagg aaagcctgac 601 aaacatatgt agattgttta tagcactgaa aggagcgata tcgtggcaat aagatatttg 661 cacagtcttt caataaaatc atttccggtt gtggaaatta ttcaggctgt caaaaaatca 721 tgtttttcac aatcattaag aagcaagtct ttacttcctt attatcaaaa acctgtcttg 781 attttatgtg aaagaaaatc ctggtgagtt aggtttgttc atccactctg acaagttaat 841 atttaattaa ttcgtcttat gaatatttgt gcctgaattt ttctggcaaa atttatgggg 901 gaaattttgc ttaaaatggc catagtaacc tttcgcgacc tatttacagt atatttttta 961 caatattgta attatatgaa aatacctata ttaattgggt cgagcagtgg cgaaagtcac 1021 tgttaataat agatggataa accatacaag gaggtgagcg ggaatgactg aaacactggt 1081 aaacgaattg gagctgcagc aagaggaaac ccaaagaaac aaatatctta ccttcgttgt 1141 tgaaaaggag tactatggga ttgaaatcag gtatgttaca gaaatcatag gaatccagtc 1201 cattacagaa gtgccggatc tgcctgaata tattcgcggg ataataaatc tcaggggaaa 1261 gataattccg gtgatggatg taagactgag atttggcaaa aagccaagag agtatgatga 1321 cagaacatgc ataatcgtca ttgacattaa cgacatgttc gttggactga ttgtagacag 1381 cgtgtgtgag gtaatgtcca ttcctgacgg ggagattgtt gtcccgcccg attttgacca 1441 aaacgagaac agatttatca aaggcatagg caaaaccgga aatgaagtca ggctgatact 1501 ggactgcgaa aaacttctca aagattgaat aagtgcaata ctcagggaat gaaatataaa 1561 aaatcaagga ggattgtata atgcggtggt atttaaattt aaaaatatca gcaaaattga 1621 ttattgcctt tttattgctt gcggcaattg cagcattagt cggtggcgtt gctcttgtaa 1681 acatcaataa catgagcaga gaggatgtgt ttttgtatga aaataacaca ctgggaatta 1741 gttacgccgc cgatgctgcc ctgcagtttc aaaggatgag gtttaacggc gcaaggatgc 1801 tgttgtttga tgaggtggaa aagcaaaacg aatctatcgg gaaaattgaa gaacatattg 1861 taaatgtgga gaaatactta agtttgtatg aggcggcaat tacaaatgag actgaccgtc 1921 agctgtttga ggatttgaag cctaaatggg aaaagtataa gtctgtgatt acaaaagaaa 1981 ctgagtatgt taaagcagga caattggaag aggcaaaaac gttgctcctt ggagatatgg 2041 ctgatgccgg tgacacattg agggatgctt ttgaagattt gatggaatat aatacaactg 2101 cagcgaagga aaaagtggat gaaaacaagc aagttgcgtc aactgcttca actataatga 2161 taaccgtgat atttgcaggc atattaatag ctattgcttt gggagtgttt atatccagga 2221 ttatcagcag acctatcgca cagatggtgg aagctgccga caggcttgcc cttggagacg 2281 tggaagtgga tgtcaaggct gaaaccaggg atgaaatagg aaaactggcc gaatctttca 2341 aaagaatgat agaaaatatc cgtgaacagg cgtatgtagt agaaagaatt gctgcgggag 2401 acatgactgt cgatgtaaga gtgagatccg acaaagactt gctgggtaaa aaacttaagg 2461 aaatggttga tacaaataat gaagtgcttt caaatatcaa tgaagttgct gcacaggtgg 2521 cagcaggagc aaaacaggta tccgactcaa gcatgcagct ttcgcaagga gcaactgagc 2581 aggcaagctc gatagaagag ctgacagctt cccttgaaca ggtggcgaat cagacacagc 2641 ttagtgccaa gaatgcgaat caggccaatg aactggctga agttgcaaaa aacaatgcag 2701 agcaaggaaa caagcaaatg gctgaaatgc tcaatgccat ggaggaaatc aataattctt 2761 catcaaatat ctccagaatt atcaaagtaa tagacgaaat tgcgttccag accaatattc 2821 ttgcact //