LOCUS NZ_ACVX01000077 2289 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00185, whole genome shotgun sequence. ACCESSION NZ_ACVX01000077 NZ_ACVX00000000 VERSION NZ_ACVX01000077.1 GI:256006164 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000077. FEATURES Location/Qualifiers source 1..2289 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene complement(<1..98) /locus_tag="ClothDRAFT_2944" CDS complement(<1..98) /locus_tag="ClothDRAFT_2944" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05431081.1" /db_xref="GI:256006165" /translation="MYKSKNLAAKVLSTLLIFITVISLINMEAPAAS" gene 350..1027 /locus_tag="ClothDRAFT_2945" CDS 350..1027 /locus_tag="ClothDRAFT_2945" /inference="protein motif:PFAM:PF00072" /note="PFAM: response regulator receiver; transcriptional regulator domain protein; SMART: response regulator receiver; KEGG: pca:Pcar_3081 two component signal transduction response regulator" /codon_start=1 /transl_table=11 /product="two component transcriptional regulator, winged helix family" /protein_id="ZP_05431082.1" /db_xref="GI:256006166" /db_xref="InterPro:IPR001789" /db_xref="InterPro:IPR001867" /translation="MKILLVEDEVRLSEALVHTLKKNNYIVDVAFDGITGQQMAETNM YNVIILDRMLPKKDGLDVLRDLRKQGVTTPVLILTAKDSVKNRVEGLDSGADDYLTKP FSKSELLARIRALGRRQSEIFVNEEINIGNMSFNCMKGEIKVNGQTIKLTSKESQILE ILIKNKNIVVSKEQLLEKVWGFQTDIELNNIEVYLSYLRKKLSKLDCGIVIETIRARG YCLKEAL" misc_feature 350..1018 /locus_tag="ClothDRAFT_2945" /note="Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Signal transduction mechanisms / Transcription]; Region: OmpR; COG0745" /db_xref="CDD:31088" misc_feature 359..694 /locus_tag="ClothDRAFT_2945" /note="Signal receiver domain; originally thought to be unique to bacteria (CheY, OmpR, NtrC, and PhoB), now recently identified in eukaroytes ETR1 Arabidopsis thaliana; this domain receives the signal from the sensor partner in a two-component systems...; Region: REC; cd00156" /db_xref="CDD:29071" misc_feature order(368..373,500..502,524..526,584..586,641..643, 650..655) /locus_tag="ClothDRAFT_2945" /note="active site" /db_xref="CDD:29071" misc_feature 500..502 /locus_tag="ClothDRAFT_2945" /note="phosphorylation site" /db_xref="CDD:29071" misc_feature order(509..514,518..526) /locus_tag="ClothDRAFT_2945" /note="intermolecular recognition site; other site" /db_xref="CDD:29071" misc_feature 650..658 /locus_tag="ClothDRAFT_2945" /note="dimerization interface; other site" /db_xref="CDD:29071" misc_feature 731..1012 /locus_tag="ClothDRAFT_2945" /note="Effector domain of response regulator. Bacteria and certain eukaryotes like protozoa and higher plants use two-component signal transduction systems to detect and respond to changes in the environment. The system consists of a sensor histidine kinase...; Region: trans_reg_C; cd00383" /db_xref="CDD:29475" misc_feature order(803..805,860..865,917..919,926..928,947..952, 986..988,1001..1003) /locus_tag="ClothDRAFT_2945" /note="DNA binding site" /db_xref="CDD:29475" gene 1029..2240 /locus_tag="ClothDRAFT_2946" CDS 1029..2240 /locus_tag="ClothDRAFT_2946" /inference="protein motif:PFAM:PF02518" /note="PFAM: ATP-binding region ATPase domain protein; histidine kinase A domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase A domain protein; KEGG: sensory histidine protein kinase" /codon_start=1 /transl_table=11 /product="histidine kinase" /protein_id="ZP_05431083.1" /db_xref="GI:256006167" /db_xref="InterPro:IPR003594" /db_xref="InterPro:IPR003661" /db_xref="InterPro:IPR004358" /db_xref="InterPro:IPR005467" /translation="MFKKLKIKFVMTNIVSITTILVIIFFGIYLSVKAFLKLQADIIL YTIANEEKLNSNFDSGFVRFFSIKIDTSGKIIGYLMNINISSEEMETLKEKVIEKGET RGKISNDKFKFKFLKIPKEYGYIIVFLDYTVEEKMYKPLIIISIYIVLLSIVLVFTVS FFLANRSIKPIKTSWEKQTAFIADASHELRTPLAVINSNLEIVMENENETVGSQMKWL GNIQSELERMKKLVDDLLFLARADAEDEMPKEYFDLSRLVHKIYDEFTPLCQKKSLEF LLDAKDNIVFYGNEFRIKQLITILLDNAIKFTGEGGKIILKLKVHANSIQLSVSDTGE GIAKEHIDKIFDRFYRVDKSRSRNHGGSGLGLAIAKCIVNEHKGTIDVFSEVSRGTEF TVSLPYKASQC" sig_peptide 1029..1133 /locus_tag="ClothDRAFT_2946" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.893) with cleavage site probability 0.460 at residue 35" misc_feature 1554..1748 /locus_tag="ClothDRAFT_2946" /note="Histidine Kinase A (dimerization/phosphoacceptor) domain; Histidine Kinase A dimers are formed through parallel association of 2 domains creating 4-helix bundles; usually these domains contain a conserved His residue and are activated via trans-...; Region: HisKA; cd00082" /db_xref="CDD:119399" misc_feature order(1569..1571,1581..1583,1593..1595,1602..1604, 1614..1616,1623..1625,1677..1679,1689..1691,1698..1700, 1710..1712,1719..1721,1731..1733) /locus_tag="ClothDRAFT_2946" /note="dimer interface; other site" /db_xref="CDD:119399" misc_feature 1587..1589 /locus_tag="ClothDRAFT_2946" /note="phosphorylation site" /db_xref="CDD:119399" misc_feature 1905..2216 /locus_tag="ClothDRAFT_2946" /note="Histidine kinase-like ATPases; This family includes several ATP-binding proteins for example: histidine kinase, DNA gyrase B, topoisomerases, heat shock protein HSP90, phytochrome-like ATPases and DNA mismatch repair proteins; Region: HATPase_c; cd00075" /db_xref="CDD:28956" misc_feature order(1923..1925,1935..1937,1944..1946,2013..2015, 2019..2021,2025..2027,2031..2036,2115..2126,2172..2174, 2178..2180,2193..2198,2202..2204) /locus_tag="ClothDRAFT_2946" /note="ATP binding site; other site" /db_xref="CDD:28956" misc_feature 1935..1937 /locus_tag="ClothDRAFT_2946" /note="Mg2+ binding site; other site" /db_xref="CDD:28956" misc_feature order(2025..2027,2031..2033,2115..2117,2121..2123) /locus_tag="ClothDRAFT_2946" /note="G-X-G motif; other site" /db_xref="CDD:28956" ORIGIN 1 gacgcagccg gtgcttccat atttattaac gatataaccg taatgaagat taatagagtt 61 gataatactt ttgctgctaa atttttcgat ttgtacatct tatctcctcc ttatcaaaaa 121 tatatatttt tatgcaacat cattttaaaa aatcaatctt aatcttttct taaaaaaagc 181 ctaaaaaata aaaaagcttt gaaaccaaat ttctctcagt cattgagata aattattgag 241 ataatataaa aaatacggta ttttatgata aaatagtaca tgtaaagaca acgcttcatc 301 aaaaaataca aaagagagcc ttttgatttt gggaaaggat gaatttaaaa tgaaaatact 361 gcttgtcgaa gatgaagtac gcctttccga agcacttgta catactttaa aaaagaacaa 421 ttacatagtt gacgtcgctt ttgacgggat aacaggccag cagatggctg agacaaatat 481 gtacaacgtc attatccttg acagaatgtt gcccaaaaaa gacgggcttg acgttttaag 541 agatctcaga aaacagggtg tcacaactcc tgttctgatt ctaaccgcca aagattcggt 601 aaaaaacaga gttgaaggtc ttgacagcgg cgccgacgat tatctgacaa aacctttttc 661 caaaagcgaa ttacttgcaa gaatacgagc tttgggaaga agacaaagcg aaatatttgt 721 aaatgaagaa ataaatatag gaaacatgag ctttaactgc atgaagggag aaataaaagt 781 aaacgggcaa accataaaac ttacgtcaaa agaatcgcaa attttggaaa tactcattaa 841 aaacaaaaac atagtggttt caaaagagca actccttgag aaagtatggg gctttcaaac 901 agatattgag cttaacaata ttgaagtata tctctcatat cttagaaaaa aactttcaaa 961 actggactgc ggaattgtga ttgagacaat aagagcaagg ggctattgcc ttaaggaggc 1021 actgtaatat gtttaaaaag cttaaaataa aatttgtaat gaccaatatt gtcagcataa 1081 caaccattct ggttattatt ttttttggta tatatttgtc agtaaaggcg tttttgaaac 1141 ttcaggccga tataatactg tacaccattg caaatgaaga aaaactaaat tcaaattttg 1201 attccggatt tgtaagattc ttttctataa aaatagacac atcaggaaaa attatcgggt 1261 atctgatgaa tatcaacatt tccagtgaag aaatggaaac actcaaagaa aaagtaatag 1321 aaaaaggaga aacaagagga aaaatttcaa atgacaagtt caaatttaaa tttttgaaaa 1381 ttcccaagga atatggatat ataattgtat ttcttgatta cactgtagaa gaaaaaatgt 1441 acaaaccact cattatcata agtatctata ttgttctatt gtccatagta ctggttttta 1501 cagtaagttt tttccttgca aacagatcca taaaaccaat aaaaacctcc tgggaaaagc 1561 agactgcttt tattgctgac gcatcccatg aactcaggac acctctcgca gtaataaatt 1621 ccaacctgga aatagtgatg gaaaacgaaa atgaaactgt cggaagtcaa atgaagtggc 1681 ttggtaacat ccaaagcgaa ttggagcgca tgaaaaaact tgttgacgat ttattgtttc 1741 tggcaagagc ggatgctgaa gatgaaatgc ctaaggaata ttttgattta agcaggcttg 1801 tacacaaaat ttatgacgag tttacacccc tttgccaaaa gaaaagcttg gaatttttat 1861 tggacgctaa agacaatatt gtgttttacg gcaacgaatt tcgcataaaa cagctcataa 1921 caatattatt ggacaatgca ataaagttca cgggtgaagg aggaaaaatc atacttaagt 1981 taaaagtgca tgcaaacagt attcaattgt ctgtcagcga tacaggagaa ggcattgcca 2041 aagaacatat tgacaaaatt tttgacagat tttacagggt ggacaaatcc cgttcacgaa 2101 accacggagg ctcgggattg ggcttggcca ttgccaaatg catagtaaat gaacataaag 2161 gcaccatcga tgttttcagt gaagtgtcca gaggaacgga atttacagta tctttgccat 2221 ataaagcatc ccagtgttaa ataataaaat atccttttac aaactcaaag catttgcatg 2281 ccgcaaaat //