BioLegato - Bugs and Workarounds
Oct. 21, 2014
1. Circular sequences
Problem: Aside from GenBank format, virtually none of the common file formats has a mechanism to designate whether a DNA/RNA sequence is circular or linear.2. Save As .... GenBank
- Reading files: Read the sequence in using File --> Open, NOT Import Foreign Format.
- Changing topology: Edit --> GetInfo. Set topology to Circular or Linear. Click on "Update".
- Use File --> Export Foreign format to export to a format that explicitly allows for specification of a circular topology. Only GenBank or EMBL formats have the ability to specify topology.
Problem: A pseudo-GenBank file is generated, omitting most of the data normally in a GenBank file.
Workaround: If you need to create a true GenBank file, use SEQUIN.
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