LOCUS PBI101TD 5349 bp DNA linear SYN 06-MAY-2008 DEFINITION GUS gene fusion vector pBI101 T-DNA region. ACCESSION U12639 VERSION U12639.1 GI:2088506 KEYWORDS pBI101; T-DNA; GUS gene fusion vector; neomycin phosphotransferase; beta-glucuronidase. SOURCE Cloning vector pBI101 ORGANISM Cloning vector pBI101 other sequences; artificial sequences; vectors. REFERENCE 1 (sites) AUTHORS Jefferson,R.A., Burgess,S.M. and Hirsh,D. TITLE beta-Glucuronidase from Escherichia coli as a gene-fusion marker JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83 (22), 8447-8451 (1986) PUBMED 3534890 REFERENCE 2 (bases 2497 to 2556) AUTHORS Jefferson,R. TITLE Assaying chimeric genes in plants: the GUS gene fusion system JOURNAL Plant Mol. Biol. Rep. 5, 387-405 (1987) REFERENCE 3 (bases 1 to 5349) AUTHORS Jefferson,R.A., Kavanagh,T.A. and Bevan,M.W. TITLE GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants JOURNAL EMBO J. 6 (13), 3901-3907 (1987) PUBMED 3327686 REFERENCE 4 (bases 1 to 5349) AUTHORS Wei,W. and Lindsey,K. TITLE T-DNA sequence of the GUS gene fusion vector pBI101 JOURNAL Unpublished REFERENCE 5 (bases 1 to 5349) AUTHORS Wei,W. TITLE Direct Submission JOURNAL Submitted (22-JUL-1994) Wenbin Wei, Botany, University of Leicester, University Road, Leicester, LE1 7RH, UK COMMENT On May 14, 1997 this sequence version replaced gi:529328. FEATURES Location/Qualifiers source 1..5349 /organism="Cloning vector pBI101" /mol_type="other DNA" /db_xref="taxon:36566" /note="sequence from the right border to the left border" misc_feature 1..2497 /note="pBin19 sequence, containing a neomycin phosphotransferase gene driven by nos promoter" repeat_region 1..25 /note="the right border repeat" CDS 385..1179 /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AAC53707.1" /db_xref="GI:2102680" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_feature 2497..2537 /note="polylinker" CDS 2551..4359 /note="modified beta-glucuronidase gene coding region from pRAJ260" /codon_start=1 /transl_table=11 /product="beta-glucuronidase" /protein_id="AAC53706.1" /db_xref="GI:529330" /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI KELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDA HTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ" 3'UTR 4430..4682 /note="3'UTR of the nopaline synthase gene" misc_feature 4686..5349 /note="pBin19 sequence (see also GenBank Accession Number U09365)" repeat_region 5325..5349 /note="the left border repeat" ORIGIN 1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac 61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg 121 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc 181 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa 241 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt 301 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc 361 tgcaccggat ctggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg 601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 901 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc 961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 1021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 1081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 1141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa 1201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 1261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 1321 gggatctcat gctggagttc ttcgcccacg ggatctctgc ggaacaggcg gtcgaaggtg 1381 ccgatatcat tacgacagca acggccgaca agcacaacgc cacgatcctg agcgacaata 1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga 1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga 1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg 1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca 1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca 1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg 1801 tgtcatctat gttactagat cgggcctcct gtcaatgctg gcggcggctc tggtggtggt 1861 tctggtggcg gctctgaggg tggtggctct gagggtggcg gttctgaggg tggcggctct 1921 gagggaggcg gttccggtgg tggctctggt tccggtgatt ttgattatga aaagatggca 1981 aacgctaata agggggctat gaccgaaaat gccgatgaaa acgcgctaca gtctgacgct 2041 aaaggcaaac ttgattctgt cgctactgat tacggtgctg ctatcgatgg tttcattggt 2101 gacgtttccg gccttgctaa tggtaatggt gctactggtg attttgctgg ctctaattcc 2161 caaatggctc aagtcggtga cggtgataat tcacctttaa tgaataattt ccgtcaatat 2221 ttaccttccc tccctcaatc ggttgaatgt cgcccttttg tctttggccc aatacgcaaa 2281 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 2341 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 2401 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 2461 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac 2521 tctagaggat ccccgggtgg tcagtccctt atgttacgtc ctgtagaaac cccaacccgt 2581 gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt 2641 gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt 2701 tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag 2761 cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg 2821 gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc 2881 tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc 2941 accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc 3001 gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccggaatc 3061 catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg 3121 acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt 3181 gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact 3241 agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat 3301 gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc 3361 atccggtcag tggcagtgaa gggccaacag ttcctgatta accacaaacc gttctacttt 3421 actggctttg gtcgtcatga agatgcggac ttacgtggca aaggattcga taacgtgctg 3481 atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat 3541 tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat 3601 gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag 3661 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag 3721 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt 3781 gccaacgaac cggatacccg tccgcaagtg cacgggaata tttcgccact ggcggaagca 3841 acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct 3901 cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg 3961 tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga acttctggcc 4021 tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga tacgttagcc 4081 gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat 4141 atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc 4201 gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa agggatcttc 4261 actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg gactggcatg 4321 aacttcggtg aaaaaccgca gcagggaggc aaacaatgaa tcaacaactc tcctggcgca 4381 ccatcgtcgg ctacagcctc gggaattgct accgagctcg aatttccccg atcgttcaaa 4441 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat 4501 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt 4561 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa 4621 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga 4681 tcgggaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 4741 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 4801 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc 4861 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 4921 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt 4981 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 5041 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 5101 ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt 5161 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg 5221 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt 5281 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat 5341 atcctgcca //