PLNT2530 PLANT BIOTECHNOLOGY

2024 COURSE OUTLINE

 Jan. 8, 10
2
 Overview of Course, Introduction

 
1.
Plant regeneration through tissue culture
Jan. 12

3
 2. Review of nucleic acids
Jan. 15 (Web)

Bioinformatics I - Introduction to Linux
Jan. 17, 19

2. Review of nucleic acids (cond.)
Jan. 22, 24, 26
3
 3. Methods of DNA manipulation
Jan. 29, 31
2
 4. Nuclear Gene Structure and Expression
Feb. 2, 5
2
5. Genomes: Organization and Comparisons
Feb. 7, 9, 12

3
 6. Gene Cloning, Mapping and Sequencing
A. Vectors
B. Libraries (cDNA, Genomic)
C. Gene isolation based on a known gene product
Feb. 14
1
6. Gene Cloning, Mapping and Sequencing
D. Gene isolation when the gene product is unknown
Feb. 16 (Web)

Bioinformatics II: Searching for and retrieving sequences from NCBI
Feb. 19 -23

 Winter Term Break
Feb. 26
1
D. Gene isolation when the gene product is unknown (cond.)
Feb. 28

Mid Term Exam
Mar. 1

6. Gene Cloning, Mapping and Sequencing (cond.)
E. DNA Sequencing
Mar.  4, 6
2
 7. Genomics, Transcriptomics, Proteomics, Metabolomics
March 8
1
  8. Genetic Engineering - Plant Transformation
A. Agrobacterium
March 11 (Web)
1
 Bioinformatics III - Simulated Cloning
March 13, 15
2
8. Genetic Engineering - Plant Transformation (cond.)
B. Direct gene transfer (biolistic, electroporation)
C. Factors influencing expression of transgenes
Mar. 18
1
 9. Genome Editing
Mar. 20, 22, 25
3
 10. Applications of Genetic Engineering in Plants
A.Herbicide tolerance genes in weed control
B. Insecticidal proteins
Mar. 27

10. Applications of Genetic Engineering in Plants (cond.)
C. Novel proteins
Mar. 29
Good Friday (University closed)
Apr. 1 (Web)
1
 Bioinformatics IV - In-silico cloning of genes by PCR
Apr. 3, 5
2
 10. Applications of Genetic Engineering in Plants (cond.)
D. Novel traits
April 8, 10
2
 11. Substantial Equivalence


FINAL EXAM - Saturday April 20, 13:30 - 15:30
Location: TBA


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