EXPERIMENT: Semi-Conservative Replication 5-bromo-deoxyuridine
(BUdr, BrdU) can incorporate into DNA in place of Thymidine. BuDR
can be detected by red flourescence when irradiated with blue
light. Cells are grown in culture for one generation with BrdU,
and then transferred to fresh culture without dye. They then are
allowed to go for one more cell division. The protocol is
illustrated below:
Red solid lines
indicate how BUdr is distributed in newly-synthesized DNA strands.
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Unless otherwise cited or
referenced, all content on this page is licensed under
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