1. A restriction map of the Shrunken locus in maize,
and its immediate vicinity, looks like this:
The location of the Shrunken gene, and its direction
of transcription are indicated by the arrow.
The wild type (S) is dominant to shrunken (s). SS
plants were crossed with ss plants to produce an F1
generation. DNA was extracted from both parents and from five F1
plants, digested with the restriction enzyme Bgl1, electrophoresed
and the DNA transferred to a nylon membrane by Southern blotting.
The blot was hybridized a labeled probe taken from the location
shown above. Draw the pattern of bands that would be seen in
the resultant autoradiogram.
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