PLNT3140 Introductory Cytogenetics

Teaching Assistant Schedule - 2021

September

Sun
Mon
Tue
Wed
Thur
Fri
Sat




2


3

1:00
meet with AP, YL in lab

Plant 150 faba beans (Yong)

4
5

6

LABOR DAY

7
This week: clean and organize lab
8

FIRST DAY OF CLASSES
9

PLNT3140 begins online


10

if faba roots ready, harvest, fix and store in fridge.
11
12

13


14

Faba (first batch) - test staining for mitosis
15

Work up procedures for lab setup and take down
in Rm. 339 and 343
16

17

harvest 2nd batch of roots
18
19
20

Intro. to Lab (YL)
21

Intro. to Lab (AP)
22

Intro. to Lab (YL)

Harvest 3rd batch of roots
23

trannsfer roots to Farmer's (BF)
24

Transfer roots to EtOH (BF)
test of staining for Faba roots

afternoon: Work up Mitosis lab (BF,YL,AP)

Karyotype lab: begin barley germination in dark (YL)
25
26

27



Karyotype - harvest roots and place in ice water? (YL,AP)
Make Fuelgen stain; 45% HOAc (BF
)

28



Karyotype - transfer to Farmer's fixative
29



Karyotpye - transfer to EtOH
test Fuelgen staining (BF)

30
1
2

October

Sun
Mon
Tue
Wed
Thur
Fri
Sat
3
4

5

6

7


8



9
10

11



12


13


14




15


16
17

18
Mitosis (YL)

19
Mitosis (YL)
20
Mitosis (AP)

21

22
23
24
25

Karyotype (YL)
26

Karyotype (AP)
27

Karyotype (AP)
28
28
30
26

27

Meiosis ()
28

Meiosis ()
29

Meiosis ()
30
1
2