- Morphological Techniques 3 cr hr.
Graduate students will have the opportunity to learn basic fundamental principles of
histological processing of research tissues for paraffin including embedding, sectioning and
Hematoxylin and Eosin staining. Colorimetric immunodetection of parafin scections will
highlight differences in IHC and routine histological stains.
Students will receive their own samples for a more hands on experience. Students will learn
how to design experimental protocols, make modifications, troubleshoot, and quantify their
results. Basic bright field microscopy, followed by analysis of results using software will help
the students see the full workflow.
Students will have a combination of lectures and laboratory time where they will apply the
lecture material in a practical setting. The couse will be divided into two blocks each with a
lecture exam and laboratory assessment grade.
- HEAL 4640
- Histology Module - Basic Histology, IHC/IF and Microscopy
Undergraduate students will learn basic fundamental principles of paraffin processing for
research tissues, embedding and sectioning. Students will preform routine Hematoxylin and
Eosin staining, see examples of other common histology stains, and have an opportunity to
view a slide library. Students will preform immunofloursecence or immunohistochemistry on
cells or tissue prepared for them. They will learn how to design experimental protocols, make
modifications, troubleshoot, and quantify their results. Students will have opportunities for both
bright field and flourescent imaging.
There will be theory evaluations to ensure their understanding of the concepts taught.
ELECTRON MICROSCOPY PLATFORM
- Medical Laboratory Assistant
Students from Robertson College’s accreddited MLA program attend an Introduction
to Histological techniques course twice annually.
Here students learn the basics of cytology, buccal swabs and staining with methyl
green and/or Papanicolaou, and blood smear staining with Wright-Giemsa. Students
proceed to basic paraffin and cryo sample processing, embedding, sectioning, staining
and imaging. Students learn standard Hematoxyling and Eosin staining on their own
Students are instructed by our facility technicians and supervised on all
equipment. Students learn theory about the techniques as well as
practice hands on skills. Students also prepare their own samples and slides as well
as taking their own images on microscopes from our imaging facility.