Transmission Electron Microscopy
We provide TEM services for tissues, cells and nano-particles. Tissue and cell samples are typically
embedded in Embed 812 and sectioned with a diamond. Thick sections are 0.5-1um and stained with
toluidine blue for orientation, quality control and documentation Thin sections are 90-100nm collected
on grids. Staining is done using Uranyl Acetate or Uranyless and Lead Citrate. Imaging is done at 800 -
To set up a consultation to discuss your specific request please contact the histology lab. Please make
arrangements with histology staff PRIOR to sample drop off so we can be available during this time.
Carefully read the guidlines below and ensure you are bringing properly prepared samples, required
forms, and billing information.
EM Sample Preparation Guidelines:
For ideal sample processing tissues must be properly prepared. Please prepare your tissue as follows
(contact Histology lab staff for any questions):
Size - Grossed tissues into 1mm cubes before fixation.
- Provide only tissue significant to your research having grossed out any other attached tissue or
organs that are not necessary
- If you deal with larger specimens, please provide only a biopsy
- Cells may be delivered to the histology lab in culture dishes or pellets depending on the request,
at a pre-arranged time.
Please fix all tissue or cells in 3% Glutaraldehyde in 0.1 M Sorensen’s buffer (This can be provided by
the Histology lab, please make arrangements for fixative pick-up in advance of cell harvest or animal
sacrifice so we can ensure the solutions are fresh). If processing brain or nerve tissue, a combination of
parafomaldehyde and gluteraldehyde may be provided. If using animals, perfusion fixation is
imperative for preservation of fine structures.
- Minimum fixation time should be 2-3 hours for cells and 3-4 hours for tissue.
- Your tissue sample should be free floating in fixative of at least 10X the volume of the tissue.
Once your samples are in fixative or sucrose solution (also may be provided) they may be delivered to
the histology lab at a pre-arranged appointment time with the completed paperwork; Work Request,
Sample Submission, and Electron Microscopy Questionaire - Waiver Form.
Electron Microscopy Service Pricing
Fixing tissue or cells that arrive (already grossed) in fixative,
sucrose buffer through to embedding in plastic
Fixative, sucrose, buffer,
osmium tetroxide, alcohol
dehydration, propylene oxide,
1-10 blocks = $195.00
5 hours per tissue set
6 hours per cell set
Processing and embedding
tissue for EM
Thick sectioning and staining with toluidine blue,
thin sectioning 90-100nm and placing on desired number of grids
(200 or 300 mesh copper)
Nickel or carbon/formvar available upon special request
Uranyl acetate, lead citrate
Uranyless, lead citrate
Staining grids for imaging
Image the grids using Phillips CM10 microscope
Taking the requested number of images at each magnification
Digital image transfer via online platorm
Saving image to USB for client
cost of USB
diamon knife, slides,
Chemicals Cost For Service
ELECTRON MICROSCOPY PLATFORM
Please contact the histology lab for customized industry pricing
$75.00/hour U of M
TEM imaging done by histology staff
800, 1100, 1450, 1950, 2600, 3400, 4600, 5800, 7900
10500, 13500, 19000, 25000, 34000, 46000, 64000, 92000
Advantage HR-B Digital CCD - Black and White
Room 14, Anatomy Department