© Histology Services, Imaging Facility & Electron Microscopy Platform, University of Manitoba, Canada,  2022

Transmission Electron Microscopy

We provide TEM services for tissues, cells and nano-particles. Tissue and cell samples are typically embedded in Embed 812 and sectioned with a diamond. Thick sections are 0.5-1um and stained with toluidine blue for orientation, quality control and documentation Thin sections are 90-100nm collected on grids. Staining is done using Uranyl Acetate or Uranyless and Lead Citrate. Imaging is done at 800 - 64,000X magnification. To set up a consultation to discuss your specific request please contact the histology lab. Please make arrangements with histology staff PRIOR to sample drop off so we can be available during this time. Carefully read the guidlines below and ensure you are bringing properly prepared samples, required forms, and billing information.

EM Sample Preparation Guidelines:

For ideal sample processing tissues must be properly prepared. Please prepare your tissue as follows (contact Histology lab staff for any questions): Size - Grossed tissues into 1mm cubes before fixation. - Provide only tissue significant to your research having grossed out any other attached tissue or organs that are not necessary - If you deal with larger specimens, please provide only a biopsy - Cells may be delivered to the histology lab in culture dishes or pellets depending on the request, at a pre-arranged time. Please fix all tissue or cells in 3% Glutaraldehyde in 0.1 M Sorensen’s buffer (This can be provided by the Histology lab, please make arrangements for fixative pick-up in advance of cell harvest or animal sacrifice so we can ensure the solutions are fresh). If processing brain or nerve tissue, a combination of parafomaldehyde and gluteraldehyde may be provided. If using animals, perfusion fixation is imperative for preservation of fine structures. - Minimum fixation time should be 2-3 hours for cells and 3-4 hours for tissue. - Your tissue sample should be free floating in fixative of at least 10X the volume of the tissue. Once your samples are in fixative or sucrose solution (also may be provided) they may be delivered to the histology lab at a pre-arranged appointment time with the completed paperwork; Work Request, Sample Submission, and Electron Microscopy Questionaire - Waiver Form.

Electron Microscopy Service Pricing

Fixing tissue or cells that arrive (already grossed) in fixative, sucrose buffer through to embedding in plastic
Fixative, sucrose, buffer, osmium tetroxide, alcohol dehydration, propylene oxide, EmBed 812
1-10 blocks = $195.00 $40.00/hour labour 5 hours per tissue set 6 hours per cell set
Processing and embedding tissue for EM
Sectioning (Diamond knife)
Thick sectioning and staining with toluidine blue, thin sectioning 90-100nm and placing on desired number of grids (200 or 300 mesh copper) Nickel or carbon/formvar available upon special request
Uranyl acetate, lead citrate or Uranyless, lead citrate
$50.00/hour labour
Staining grids
Staining grids for imaging
Imaging
Image the grids using Phillips CM10 microscope Taking the requested number of images at each magnification per sample
Digital Transfer USB
Digital image transfer via online platorm Saving image to USB for client
cost of USB
no charge $8.00/USB
diamon knife, slides, toluidine blue, grids
$50.00/hour labour

Task

Description

Chemicals Cost For Service

$50.00/hour labour
HISTOLOGY SERVICES IMAGING FACILITY ELECTRON MICROSCOPY PLATFORM
Please contact the histology lab for customized industry pricing
Electron Microscope Phillips CM10
$75.00/hour U of M $120.00/hour External
TEM imaging done by histology staff

Phillips CM10

Description Software Objective Control Stage Control Capabilities Magnification Light Source Camera Location
Electron Microscope AMT 600 Manual Manual Electron Microscopy 800, 1100, 1450, 1950, 2600, 3400, 4600, 5800, 7900 10500, 13500, 19000, 25000, 34000, 46000, 64000, 92000 Tungsten Advantage HR-B Digital CCD - Black and White Room 14, Anatomy Department