We offer standard paraffination preparation
To set up a consultation to discuss your specific needs or ask any additonal questions please contact
the histology lab. Please make arrangements with histology staff PRIOR to sample drop off so we can
be available to accept your samples. Carefully read the guidlines below and ensure you are bringing
properly prepared samples, and all required paperwork and approvals.
Paraffin Sample Preparation Guidelines:
For best reults tissues must be properly prepared.
Size - Samples must be grossed to a reasonable size BEFORE fixation. Tissues should be no thicker
than 5mm and a maximum of 1.5cm length and width. Smaller pieces have better fixation and are
easier to section.
Provide only tissue significant to your research, having grossed out any other attached tissue or organs
that are not necessary. If you deal with larger specimens, please provide only a biopsy.
Fixation - please fix all tissues in appropriate fixative based on what the samples are to be used for.
Perfusion is optimal if using animal tissues, immersion also sufficient if the tissue is of an appropriate
size. Some fixatives may interfere with staining or immunohistochemistry conditions. Common fixatives
used are 10% Buffered Formalin or 4% Formaldehyde (prepared from a 37% stock liquid containing
methanol commercially purchased or prepared from a powder).
Minimum fixation time should be 24 hours and can last as long as 5 days. Your tissue sample should
be free floating in fixative of at least 10X the tissue volume.
Once your samples are in fixative, they may be delivered to the Histology Lab at a pre-arranged
appointment time with the completed Work Request, Sample Submission and Waiver Forms. Samples
may also be stored in PBS at 4°C or 70% ethanol at room temperature for short periods. Please bring a
slide box of sufficient size for the requested slides at the time of sample drop off or one will be added to
Chemicals Cost For Service
Placing tissue that arrives in fixative (already grossed)
into cassettes and labeling for the tissue processor
Processor runs overnight
using formalin, dehydrating
alcohol, Xylene and Paraffin.
1-24 cassettes = $39.00
25-50 cassettes = $78.00
51-75 cassettes = $117.00
76-100 cassettes = $156
Taking the cassettes from the tissue processor and
setting them in paraffin in the correct orientation,
then filling the mold with paraffin to create the block
Paraffin from embedding
Included in tisue processing
If separate $5.00 each
Sectioning the desired number for sections/slide from
each block as requested
knife blade included
$1.50/slide Superfrost Plus
Staining and coverslipping slides as requested
Varies depending on the
$3.00/slide H & E
for special stains
IHC, IF and
IHC or IF on the prepared slides with
antibodies as well as other requested assays (kit)
Custom pricing for
plus all required supplies
Image the slides using bright field or fluorescent microscope
taking pictures, performing analysis where required (counting,
$75.00/hour U of M
Imaging Done by Staff
Digital image transfer via online platorm
Saving image to USB for client
cost of USB
cost of box
Paraffin Service Pricing
ELECTRON MICROSCOPY PLATFORM
Please contact the histology lab for customized industry pricing