© Histology Services, Imaging Facility & Electron Microscopy Platform, University of Manitoba, Canada,  2022

Paraffin

We offer standard paraffination preparation

To set up a consultation to discuss your specific needs or ask any additonal questions please contact the histology lab. Please make arrangements with histology staff PRIOR to sample drop off so we can be available to accept your samples. Carefully read the guidlines below and ensure you are bringing properly prepared samples, and all required paperwork and approvals.

Paraffin Sample Preparation Guidelines:

For best reults tissues must be properly prepared. Size - Samples must be grossed to a reasonable size BEFORE fixation. Tissues should be no thicker than 5mm and a maximum of 1.5cm length and width. Smaller pieces have better fixation and are easier to section. Provide only tissue significant to your research, having grossed out any other attached tissue or organs that are not necessary. If you deal with larger specimens, please provide only a biopsy. Fixation - please fix all tissues in appropriate fixative based on what the samples are to be used for. Perfusion is optimal if using animal tissues, immersion also sufficient if the tissue is of an appropriate size. Some fixatives may interfere with staining or immunohistochemistry conditions. Common fixatives used are 10% Buffered Formalin or 4% Formaldehyde (prepared from a 37% stock liquid containing methanol commercially purchased or prepared from a powder). Minimum fixation time should be 24 hours and can last as long as 5 days. Your tissue sample should be free floating in fixative of at least 10X the tissue volume. Once your samples are in fixative, they may be delivered to the Histology Lab at a pre-arranged appointment time with the completed Work Request, Sample Submission and Waiver Forms. Samples may also be stored in PBS at 4°C or 70% ethanol at room temperature for short periods. Please bring a slide box of sufficient size for the requested slides at the time of sample drop off or one will be added to your invoice.

Task

Description

Chemicals Cost For Service

Placing tissue that arrives in fixative (already grossed) into cassettes and labeling for the tissue processor
Fixative, Cassettes Processor runs overnight using formalin, dehydrating alcohol, Xylene and Paraffin.
1-24 cassettes = $39.00 25-50 cassettes = $78.00 51-75 cassettes = $117.00 76-100 cassettes = $156 $40.00/hour labour
Processing Tissue for Paraffin
Embedding Tissue in Paraffin
Taking the cassettes from the tissue processor and setting them in paraffin in the correct orientation, then filling the mold with paraffin to create the block
Paraffin from embedding machine
Included in tisue processing charge If separate $5.00 each
Cutting paraffin sections
Sectioning the desired number for sections/slide from each block as requested
knife blade included
$40.00/hour labour $1.00/slide APTES $1.50/slide Superfrost Plus
Staining paraffin sections
Staining and coverslipping slides as requested
Varies depending on the requested stain
$40.00/hour labour $3.00/slide H & E SeeStaining page for special stains
IHC, IF and other Assays
IHC or IF on the prepared slides with antibodies as well as other requested assays (kit)
Custom pricing for individual requests
$100.00/run $40.00/hour labour plus all required supplies and chemicals
Imaging/analysis
Image the slides using bright field or fluorescent microscope taking pictures, performing analysis where required (counting, measuring, graphing)
$75.00/hour U of M $100.00/hour External Imaging Done by Staff
Digital Transfer USB
Digital image transfer via online platorm Saving image to USB for client
cost of USB
No charge $8.00/USB
Slide Box
Slide storage/transport
cost of box
$10.00/Box

Paraffin Service Pricing

HISTOLOGY SERVICES IMAGING FACILITY ELECTRON MICROSCOPY PLATFORM
Please contact the histology lab for customized industry pricing