© Histology Services, Imaging Facility & Electron Microscopy Platform, University of Manitoba, Canada,  2022

Structured Illumination Microscopy

High Resolution Imaging

Superresolution structured illumination microscopy (SR-SIM) is an advanced imaging technique used to image fine structural details with minimal changes to standard immunofluorescence protocols using conventional dyes. The resulting image is of high quality with up to 120 nm lateral resolution (X,Y) and 300 nm axial resolution (Z). This technique uses five different grating frequencies to allow for optimal matching of illumination pattern to laser wavelength and objective lens used. For more information about SR-SIM in our facility please contact: Dr. Thomas Klonisch Director of Histomorpological and Ultrastructural Imaging Platform Office: 789-3893 Email: thomas.klonisch@umanitoba.ca 114A-745 Bannatyne Avenue Winnipeg, MB R3E 0J9 Basic Medical Services Building Bannatyne Campus
HISTOLOGY SERVICES IMAGING FACILITY ELECTRON MICROSCOPY PLATFORM