IDENTIFY BY DATABASE SEARCH

Raw sequences are compared with DNA and protein sequence databases to identify their closest homologue. ESTs whose best match has a probability of occurrence by chance of  P > 0.01 are classified as  unknown .

raw sequence 
>pMB56-1G
CGGATCCGGAACCCTAACCGCCGCATCGGCGTCTACTACGACCAGATCGA
GGTCAGAGGATACTACGGAGATGAAAGATTCGGTAGCAGCAACGTGGCGC
CGTTCTACCAGGGGCACAAGAACACGACGGAGGTGGGGAGTAAGCTCGAG
GGACAGGGCTTGGTGGTGCTTAACGGCGGAGATCGGAAGGATCTGGACGA
GGATTTGAAGTCGGGGATATACAGGATCGACGCGAAGCTGAGGCTTAGGG
TGAGGTTTAAGTTTGGGCTGATCAAGTCGTGGAGGTTTAAGCCTAAGGTT  
AGGTGTGATCTCAAGGTTCCTCTCGGTTCCTCTAATATAACCAGCGGGTT  
TCAGTTTCAGCGGACCAAGTGTGACATTGACTTTTGATGCTAGTCAAAGT  
CAAATGCTATTGTGTTCGGATTCTATTTTTCATTTATATGCACTTGTTTA  
TCTTATCTTTAGATTGTTTTTCGTACATGGACTTTATGTGATATTGTCGG  
AGAGATATATGTTTTGTGTAATAATCTTTTAGCGTATGGTTCCATGTAAA  
TAAAATAGTTTTTCCTTGC

FASTA, TFASTA,  FASTY
FASTA (3.14 April, 1998) function (optimized, DNA matrix) ktup: 4
 join: 62, opt: 47, gap-pen: -16/ -4, width:  16 reg.-scaled
   Scan time: 234.260
   The best scores are:                             initn init1 opt z-sc E(52168)
AB006700 - Arabidopsis thaliana genomic   (79419) 1093  783 1589 1195.5 1.2e-61 
NTHIN1 - N.tabacum mRNA for hin1 gene.    ( 979)  379  192  420 324.5 3.1e-11 
SC9934 - S.cerevisiae chromosome IV cosm  (34586)  157  157  185 122.8 0.15 
SPAC1F7 - S.pombe chromosome I cosmid c1  (34397)  231   81  176 115.9 0.37 
enter into ACeDB database
submit to GenBank
LOCUS       AI352735      569 bp    mRNA            EST       30-DEC-1998
DEFINITION  MB56-1G PZ204.BNlib Brassica napus cDNA clone pMB56-1G 3' similar
            to hin1, hypersensitive response gene|Nicotiana tabacum|Y07563,
            mRNA sequence.
ACCESSION   AI352735
NID         g4089941
KEYWORDS    EST.
SOURCE      rape.
  ORGANISM  Brassica napus
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons;
            Rosidae; Capparales; Brassicaceae; Brassica.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Fristensky,B. and Balcerzak,M.
  TITLE     Expressed sequence tags from the defense response of Brassica napus
            to Leptosphaeria maculans
  JOURNAL   Unpublished (1998)
COMMENT
            Contact: Dr. Brian Fristensky
            Department of Plant Science
            University of Manitoba
            Winnipeg, Canada, R3T 2N2
            Tel: 204-474-6085
            Fax: 204-474-7528
            Email: frist@cc.umanitoba.ca
            Seq primer: T7.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Brassica napus"
                     /cultivar="Glacier"
                     /note="Organ: leaf; Vector: Uni-ZAPXR (Stratagene);
                     Site_1: EcoRI; Site_2: XhoI; mRNA from B. napus cv.
                     Glacier leaves infiltrated with Leptosphaeria maculans PG2
                     pycnidiospores for 48 hr. (incompatible interaction)."
                     /db_xref="taxon:3708"
                     /clone="pMB56-1G"
                     /clone_lib="PZ204.BNlib"
BASE COUNT      139 a    100 c    161 g    169 t
ORIGIN
        1 cggatccgga accctaaccg ccgcatcggc gtctactacg accagatcga ggtcagagga
       61 tactacggag atgaaagatt cggtagcagc aacgtggcgc cgttctacca ggggcacaag
      121 aacacgacgg aggtggggag taagctcgag ggacagggct tggtggtgct taacggcgga
      181 gatcggaagg atctggacga ggatttgaag tcggggatat acaggatcga cgcgaagctg
      241 aggcttaggg tgaggtttaa gtttgggctg atcaagtcgt ggaggtttaa gcctaaggtt
      301 aggtgtgatc tcaaggttcc tctcggttcc tctaatataa ccagcgggtt tcagtttcag
      361 cggaccaagt gtgacattga cttttgatgc tagtcaaagt caaatgctat tgtgttcgga
      421 ttctattttt catttatatg cacttgttta tcttatcttt agattgtttt tcgtacatgg
      481 actttatgtg atattgtcgg agagatatat gttttgtgta ataatctttt agcgtatggt
      541 tccatgtaaa taaaatagtt tttccttgc
//