Defense Gene | Non-Defense Gene | ||||||||||
PR10 | Non-PR10 | Total | Pea | Non-Pea | Total | ||||||
No.a | Sizeb | No. | Size | No. | Size | No. | Size | No. | Size | No. | Size |
11 | 8 kb | 38 | 43 kb | 49 | 51 kb | 29 | 26 kb | 137 | 116 kb | 166 | 142 kb |
Note: "a" number of genes in dataset; "b" size of dataset
in kilobase (kb).
For a simple example, the target sequence is (AGCT) and
the source sequence (dataset) is (AGCTAGAG). XLAND will generate a result
like this:
possible pattern | A | G | C | T | AG | GC | CT | AGC | GCT | AGCT |
frequency | 3 | 3 | 1 | 1 | 3 | 1 | 1 | 1 | 1 | 1 |
These frequencies are used to plot the sequence landscape. A peak in the landscape means a conserved DNA sequence in the dataset. The higher the peak, the longer the conserved sequence.
Step 1: Use each of the three DNA binding domains, PDA1, PDA2 and PDC1, as target sequences to scan each of 49 defense genes for shared motifs.
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