EXERCISE 1 - Segregation in the F1 generation

1. A restriction map of the Shrunken locus in maize, and its immediate vicinity, looks like this:


The location of the Shrunken gene, and its direction of transcription are indicated by the arrow.
The wild type (S) is dominant to shrunken (s). SS plants were crossed with ss plants to produce an F1 generation. DNA was extracted from both parents and from five F1 plants, digested with the restriction enzyme Bgl1, electrophoresed and the DNA transferred to nitrocellulose by Southern blotting. The blot was hybridized a labeled probe taken from the location shown above.  Draw the pattern of bands that would be seen in the resultant autoradiogram.

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