THE TOOLS OF BIOTECHNOLOGY
How does a gene work?
Transformation occurs in nature
Hitching a ride on the T-DNA
In transgenic plants, it is possible to
express a novel gene from any species
overexpress a gene
inhibit expression of a gene
(ie. "gene knockout")
express a gene in all tissues or organs
limit expression to a specific tissue, organ, or stimulus
The Problem: Keeping the genes we want and getting rid of those we don't want.
Wide cross to obtain a desireable gene
Back cross to restore the original parental genome
The Solution: Molecular Markers
Detecting specific DNA sequences: Polymerase chain Reaction
Polymorphism - difference in DNA sequences
Use of maps in marker-assisted selection
Applications of Molecular Markers
BREEDING VS. TRANSGENICS - Solutions, choices, benefits and drawbacks
For more information on biotechnology:
Biotech. Info at Agriculture and Agrifoods Canada
Ag-West Biotech Inc.
USDA Biotechnology Information Center