A LARGE ARRAY OF DEFENSE GENES IS ACTIVATED BY PATHOGENIC ATTACK

2D-protein gels allow us to visualize genes proteins induced in pea during non-host resistance to Fusarium solani f. sp. phaseoli.

Wagoner et al. (1982) Phys. Plant Pathol. 20:99-107.

Pea cDNA clones representing fungal-induced mRNAs were identified by differential hybridization using labeled probes derived from mRNAs from inoculated or uninoculated plants.
Riggleman et al. (1985) Plant Mol. Biol. 4:81-86.

Defense genes identified included

PR10 - 17 kDa ribonuclease-like protein
defensin - 5 kDa Cys-rich antifungal protein
DRR206 - 20 kDa protein, unknown function

Timecourses reveal that expression patterns differ when pea pods are inoculated with compatible or incompatible races of Fusarium solani, chitosan, or water.

Fristensky et al. (1985) Phys. Plant Pathol. 27: 15-28.