Segregating Bin Scoring Function

Scores a bin by comparing peaks to cutoffs. Let a region by the range of intensity values between two cutoffs (or a cutoff and the baseline or a cutoff and the maximum height). The segregating score function contains a label for every region. A lane is scored by assigning it a label for the highest region that contains a peak. To find out how a peak is determined, see the "Peak Locating Algorithms" heading in Program Features.

The segregating score function will also provide overall information about the bin it scored. This information includes:

• The mean of the peaks that were used to score the bin.
• The standard deviation of those peaks
• The number of times each label appears in the bin
• The number of peaks used
• The maximum number of peaks that could have been used.

The last two may differ because of manual scoring. The user can change the scoring, so if a peak would normally cause the lane to be scored in a manner contrary to the users desires, it is not included in the mean and standard deviation.

Sometimes when the program tries to score a bin, it will find that the number of cutoffs does not match the number needed by the segregating score function. In this case, the program sets the segregating score function to the correct number, but lets the user assign the labels. It is not neccessary to abjust the number of labels or cutoffs when this happens since it is already set to a value that matches the required number, but one should enter the labels.

The method has the following options:

• The number of fields.
• The labels.