Jan. 6, 8
|
2
|
Overview of Course,
Introduction
1. Plant
regeneration through tissue culture
|
Jan. 10,
13, 15
|
3
|
2. Review of nucleic
acids |
Jan. 17 (Web)
|
|
Bioinformatics I - Introduction
to Linux |
Jan. 20, 22, 24
|
3
|
3. Methods
of DNA manipulation
|
Jan.
27, 29
|
2
|
4.
Nuclear Gene Structure and Expression |
Jan. 31
Feb. 3
|
2
|
5.
Genomes: Organization and Comparisons |
Feb.
5, 7, 10
|
3
|
6.
Gene Cloning, Mapping and Sequencing
A. Vectors
B. Libraries (cDNA, Genomic)
C. Gene
isolation based on a known gene product
|
Feb. 12
|
1
|
6. Gene Cloning, Mapping and Sequencing
D. Gene isolation when the
gene product is unknown
|
Feb. 14 (Web)
|
|
Bioinformatics
II: Searching
for and retrieving sequences from NCBI |
Feb. 17 -21
|
|
Winter
Term Break
|
Feb. 24
|
1
|
D. Gene
isolation when the gene product is unknown (cond.)
|
Feb. 26
|
|
Mid Term Exam
|
Feb.
28
|
|
6. Gene Cloning, Mapping and Sequencing
(cond.)
E. DNA Sequencing
|
Mar. 3, 5
|
2
|
7. Genomics,
Transcriptomics, Proteomics, Metabolomics
|
March 7
|
1
|
8. Genetic Engineering - Plant
Transformation
A. Agrobacterium
|
March
10 (Web)
|
1
|
Bioinformatics
III - Simulated
Cloning |
March
12, 14
|
2
|
8. Genetic Engineering - Plant
Transformation (cond.)
B. Direct gene transfer
(biolistic, electroporation)
C. Factors influencing expression
of transgenes
|
Mar.
17
|
1
|
9. Genome Editing
|
Mar.
19, 21, 24
|
3
|
10. Applications of Genetic
Engineering in Plants
A.Herbicide
tolerance genes in weed control
B.
Insecticidal proteins
|
Mar. 26
|
|
10. Applications of Genetic Engineering
in Plants (cond.)
C. Novel
proteins
|
Mar.
28 (Web)
|
1
|
Bioinformatics IV -
In-silico cloning of genes by PCR
|
Mar.
31
Apr. 2
|
2
|
10. Applications of Genetic
Engineering in Plants (cond.)
D. Novel
traits
|
April 4, 7
|
2
|
11. Substantial
Equivalence
|
|
|
FINAL EXAM - TBA
Location: TBA |