PLNT2530 PLANT BIOTECHNOLOGY

2018 COURSE OUTLINE
>>> Tentative <<<

 Jan. 3 - 5
2
 Overview of Course, Introduction

 
1.
Plant regeneration through tissue culture
Jan. 8 - 12

3
 2. Review of nucleic acids
Jan. 15
1
 Bioinformatics I - Introduction to Linux (Machray 113)
Jan. 17 - 19
2
 3. Methods of DNA manipulation
Jan. 22 - 24
2
 4. Nuclear Gene Structure and Expression
Jan. 26 - 29
2
5. Genomes: Organization and Comparisons
Jan. 31, Feb. 2,5
3
 6. Gene Cloning, Mapping and Sequencing
A. Vectors
B. Libraries (cDNA, Genomic)
Feb. 7
1
Bioinformatics II: Searching for and retrieving sequences from NCBI (Machray 113)
Feb. 9, 12, 14
3
  6. Gene Cloning, Mapping and Sequencing (cond.)
C. Gene isolation based on a known gene product
D. Gene isolation when the gene product is unknown
E. DNA Sequencing
Feb. 19 - 23

Reading Week
Feb. 26

Mid Term Exam - in class
Feb.  28, Mar. 2
2
 7. Genomics, Transcriptomics, Proteomics, Metabolomics
March 5
1
 Bioinformatics III - Simulated Cloning (Machray 113)
March 7, 9, 12
3
  8. Genetic Engineering - Plant Transformation
A. Agrobacterium
B. Direct gene transfer (biolistic, electroporation)
C. Factors influencing expression of transgenes
March 14
1
9. Genome Editing
March 16
1
 Bioinformatics IV - In-silico cloning of genes by PCR (Machray 113)
March 19 - 28
5
10. Applications of Genetic Engineering in Plants
A.Herbicide tolerance genes in weed control
B. Insecticidal proteins
C. Novel proteins
D. Novel traits
E. Elimination of allergens
March 30

 Good Friday - University Closed
April 2, 4
2
11. Substantial Equivalence


Final Exam -  TBA

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