PLNT2530 PLANT BIOTECHNOLOGY

2019 COURSE OUTLINE

 Jan. 7, 9
2
 Overview of Course, Introduction

 
1.
Plant regeneration through tissue culture
Jan. 11, 14, 16

3
 2. Review of nucleic acids
Jan. 18
1
 Bioinformatics I - Introduction to Linux (Agriculture 137)
Jan. 21, 23
2
 3. Methods of DNA manipulation
Jan. 25,  28
2
 4. Nuclear Gene Structure and Expression
Jan. 30, Feb. 1
2
5. Genomes: Organization and Comparisons
Feb. 4, 6, 8
6
 6. Gene Cloning, Mapping and Sequencing
A. Vectors
B. Libraries (cDNA, Genomic)
C. Gene isolation based on a known gene product
Feb. 11
1
Bioinformatics II: Searching for and retrieving sequences from NCBI (Agriculture 137)
Feb. 13

  6. Gene Cloning, Mapping and Sequencing (cond.)
D. Gene isolation when the gene product is unknown
Feb. 18 - 22

Reading Week
Feb. 25

D. Gene isolation when the gene product is unknown (cond.)
Feb. 27

Mid Term Exam - in class
March 1

6. Gene Cloning, Mapping and Sequencing (cond.)
E. DNA Sequencing
Mar.  4, 6
2
 7. Genomics, Transcriptomics, Proteomics, Metabolomics
March 8, 11, 13
3
  8. Genetic Engineering - Plant Transformation
A. Agrobacterium
B. Direct gene transfer (biolistic, electroporation)
C. Factors influencing expression of transgenes
March 15
1
 Bioinformatics III - Simulated Cloning (Agriculture 137)
March 18
1
 9. Genome Editing
March 20, 22, 25
3
 10. Applications of Genetic Engineering in Plants
A.Herbicide tolerance genes in weed control
B. Insecticidal proteins
C. Novel proteins
March 27
1
 Bioinformatics IV - In-silico cloning of genes by PCR (Agriculture 137)
March 29, April 1
2
 10. Applications of Genetic Engineering in Plants (cond.)
D. Novel traits
E. Elimination of allergens
April 3,5
2
 11. Substantial Equivalence


Final Exam - Friday April 12, 09:00 - 11:00

Hints for printing lectures: When printing the PDF, print in portrait orientation, 2 pages per sheet.