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QUASI Alogrithm

• Developed by Dr. Jeffrey J Stewart, 2001 (BioGenetic Ventures, Seattle, WA, US)
• Published in BMC Bioinformatics (2001) 2:1
• Applied for RNA viruses
  

Principle

1. Building Consensus
   
2. Calculating the observed and expected R:S ratio (R: replace mutation; S: Silent mutation) on each aa
   
3. Conducting "two-side test of binomial distribution"